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| Feng,H.J.; Ouyang,W.; Liu,J.H.; Sun,Y.G.; Hu,R.; Huang,L.H.; Xian,J.L.; Jing,C.F.; Zhou,M.J.. |
| Hypertrophy is a major predictor of progressive heart disease and has an adverse prognosis. MicroRNAs (miRNAs) that accumulate during the course of cardiac hypertrophy may participate in the process. However, the nature of any interaction between a hypertrophy-specific signaling pathway and aberrant expression of miRNAs remains unclear. In this study, Spague Dawley male rats were treated with transverse aortic constriction (TAC) surgery to mimic pathological hypertrophy. Hearts were isolated from TAC and sham operated rats (n=5 for each group at 5, 10, 15, and 20 days after surgery) for miRNA microarray assay. The miRNAs dysexpressed during hypertrophy were further analyzed using a combination of bioinformatics algorithms in order to predict possible... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cardiac hypertrophy; Signaling pathway; Biomarker. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500361 |
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| CHEN,SONGLIN; YANG,LEI; PAN,AIPING; DUAN,SILIANG; LI,MINGFEN; LI,PING; HUANG,JINGJING; GAO,XINGXIN; HUANG,XIAOQI; LIN,YINGHUI. |
| Abstract: The aim of this study was to investigate the inhibitory effect of regulation of miR-122-MAP3K2 signal pathway on the hepatitis B cells. We detected the content of MAP3K2 from patients with HBV blood serum samples and analyzed the correlation between content of MAP3K2 and copies of HBV-DNA. Wound healing and Transwell assays were used to detect the function of cells from control group (wild type) and observer group (overexpresses miR-122). Secretion levels of HBsAg and MAP3K2 in the supernatant and level of MAP3K2 in cells were detected by ELISA and western blot, respectively. The results showed that there was a positive correlation between the copies of HBV-DNA and MAP3K2 in serum. In the assays involving detection of the number of HBV-DNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis B cells; MiR-122; Inhibit; Signaling pathway. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300703 |
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| Lopes,G.J.D.; Góis,C.C.; Lima,L.H.R.G.; Castrucci,A.M.L.. |
| Endothelins (ETs) and sarafotoxins (SRTXs) belong to a family of vasoconstrictor peptides, which regulate pigment migration and/or production in vertebrate pigment cells. The teleost Carassius auratus erythrophoroma cell line, GEM-81, and Mus musculus B16 melanocytes express rhodopsin, as well as the ET receptors, ETB and ETA, respectively. Both cell lines are photoresponsive, and respond to light with a decreased proliferation rate. For B16, the doubling time of cells kept in 14-h light (14L):10-h darkness (10D) was higher compared to 10L:14D, or to DD. The doubling time of cells kept in 10L:14D was also higher compared to DD. Using real-time PCR, we demonstrated that SRTX S6c (12-h treatment, 100 pM and 1 nM; 24-h treatment, 1 nM) and ET-1 (12-h... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rhodopsin; Sarafotoxin; Endothelin; Pigment cells; Gene expression; Signaling pathway. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900004 |
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