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Development and characterization of microsatellite loci for Campomanesia xanthocarpa (Myrtaceae) and cross amplification in related species Biological Sciences
Góes, Bruna Delgado; Ruas, Eduardo Augusto; Benício, Lucas Milanez; Cassiano, Daniele; Souza, Felipe Pinheiro de; Ruas, Paulo Maurício.
Campomanesia xanthocarpa is a native tree, of common occurrence in almost all Brazilian Forest formations, which has its fruits and timber with high commercial value. Using an enriched genomic library we isolated and characterized microsatellite loci for C. xanthocarpa (Myrtaceae), in order to estimate genetic diversity parameters for this and related species. Twenty-eight microsatellite loci were identified and ten of them successfully amplified and showed polymorphism in a sample of 96 individuals, from four natural populations. The number of alleles per locus ranged from two to eight, and the observed and expected heterozygosities varied from 0.042 to 1.000 and from 0.294 to 0.855, respectively. These markers were tested and validated in two related...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ciências Biológicas gabiroba; Genetic diversity; Molecular marker; Native tree; Simple sequence repeat (SSR) Desenvolvimento de Marcadores Simple Sequence Repeats.
Ano: 2019 URL:
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