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| Yuan,Mei; Gong,Limin; Meng,Ronghua; Li,Shuangling; Dang,Phat; Guo,Baozhu; He,Guohao. |
| Cultivated peanut (Arachis hypogaea L.) is an oilseed crop of economic importance. It is native to South America, and it is grown extensively in the semi-arid tropics of Asia, Africa, and Latin America. Given an extremely narrow genetic base, efforts are being made to develop simple sequence repeat (SSR) markers to provide useful genetic and genomic tools for the peanut research community. A SSR-enriched library to isolate trinucleotide (GGC)n SSRs in peanut was constructed. A total of 143 unique sequences containing (GGC)n repeats were identified. One hundred thirty eight primer pairs were successfully designed at the flanking regions of SSRs. A suitable polymerase was chosen to amplify these GC-rich sequences. Although a low level of polymorphism was... |
| Tipo: Journal article |
Palavras-chave: Cultivated peanut; Microsatellites; Polymorphism; Simple sequence repeat. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600005 |
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| Tang Wang,Chuan; Dao Yang,Xin; Xu Chen,Dian; Lin Yu,Shan; Zhen Liu,Guang; Yi Tang,Yue; Zhi Xu,Jian. |
| SSRs have proved to be the most powerful tool for variety identification in groundnut of similar origin, and have much potential in genetic and breeding studies. To facilitate SSR discovery in groundnut, we proposed a highly simplified SSR isolation protocol based on multiple enzyme digestion/ligation, mixed biotin-labeled probes and streptavidin coated magnetic beads hybridization capture strategy. Of the 272 colonies randomly picked for sequencing, 119 were found to have unique SSR inserts |
| Tipo: Journal article |
Palavras-chave: Groundnut; Isolation; Simple sequence repeat. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300015 |
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| Lim,Soohwan; Lee,Jeongyeo; Lee,Hyun Joo; Park,Kun-Hyang; Kim,Dae-Soo; Min,Sung Ran; Jang,Won Seok; Kim,Tae II; Kim,HyeRan. |
| ABSTRACT: Cultivated strawberry (Fragaria × ananassa Duch.) is a high value horticultural crop. In this study, the genetic diversity of 160 strawberry accessions was determined using five highly polymorphic simple sequence repeat (SSR) markers. Sixty different alleles were identified, with allele frequencies in the range of 0.006 to1. Similarity scores were in the range of 0.034 to 0.963 (average: 0.507). The accessions were categorized into five groups. Group 1 contained two diploid Fragaria vesca species and one unknown accession. Group 2 contained one accession (F x ananassa). Group 3 contained 20 F × ananassa accessions and six unknown accessions. Group 4 contained 48 F. × ananassa accessions, one octaploid Fragaria chiloensis species, and six unknown... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fragaria × ananassa; Simple sequence repeat; Cultivar identification. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000300226 |
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