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Registros recuperados: 9
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A novel genome-wide microsatellite resource for species of Eucalyptus with linkage-to-physical correspondence on the reference genome sequence. Repositório Alice
GRATTAPAGLIA, D.; MAMANI, E. M. C.; SILVA JUNIOR, O. B.; FARIA, D. A..
Tipo: Separatas Palavras-chave: Ancestry informative markers; Microsatellites; Simple sequence repeats; Eucalyptus Grandis; Eucalyptus globulus.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1034895
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Assessment of inter- and intra-cultivar variations in olive using SSR markers Scientia Agricola
Ipek,Ahmet; Barut,Erdogan; Gulen,Hatice; Ipek,Meryem.
Olive (Olea europaea L.) production in the world has been made by using many cultivars, and the genetic uniformity of commercial cultivars is important for standard olive oil and table olive production. The genetic variation among and within commonly cultivated olive cultivars in Turkey was analyzed using SSR markers. A total of 135 leaf samples were collected from 11 commonly cultivated olive cultivars from 11 provinces in four geographical regions of Turkey. Seven SSR primer pairs generated 46 SSR markers, and the number of SSR markers per primer pair ranged from 4 (UDO-14) to 9 (GAPU-89) with an average of 6.57. This high level of SSR polymorphism suggests that olive production in Turkey has been made using genetically diverse olive cultivars and this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Olea europaea L.; Microsatellite; Intra-varietal variation; Synonyms; Simple sequence repeats.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000500007
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Evaluation of genetic diversity and linkage disequilibrium in Korean-bred rice varieties using SSR markers Electron. J. Biotechnol.
Wang,Xiao-Qiang; Kwon,Soon-Wook; Park,Yong-Jin.
Background: In order to evaluate the variation among different rice types, the genetic diversity in a rice collection composed by 59 breeding lines, 23 landraces, 18 weedy rice lines, and 35 introduced lines that collected from countries worldwide was analyzed using 134 simple sequence repeat markers. Results: In total, 1264 alleles were identified (average, 9.43 per locus). Rare alleles made up a large portion (58.4%) of the detected alleles, and 29 unique alleles associated with rice accessions were also discovered. A model-based structural analysis revealed the presence of three subpopulations. The genetic relationships revealed by the neighbour-joining tree method were fairly consistent with the structure-based membership assignments for most of the...
Tipo: Journal article Palavras-chave: Landraces; Linkage disequilibrium; Population structure; Rice; Simple sequence repeats.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500011
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First genomic microsatellite markers developed for Platonia insignis (Clusiaceae), a Brazilian fruit tree. Repositório Alice
PARAENSE, L. C. R.; PENA, D. N.; DARNET, S. H.; RODRIGUES, S. de M.; MENEZES, I. C. de; CUNHA, E. F. M..
Platonia insignis is a fruit tree native of Brazil with allogamous and asexual reproduction. The production of fruits is mainly obtained by exploitation of natural populations and the impact of genetic structuring on plant production may be evaluated. For this purpose, codominant and multiallelic markers such as microsatellite are the most suitable, but they need to be developed for this species. Thus, the aim of this work was to develop and validate microsatellite markers for P. insignis. We used Roche 454 GS FLX sequencing platform of a single P. insignis genotype and 1702 microsatellite sequences were identified. Based on some pre-requisites, we could develop 50 primer pairs to be tested. Twenty-two primer pairs successfully amplified fragments and they...
Tipo: Artigo de periódico Palavras-chave: Diversidade genética; Genetic diversity; Simple sequence repeats; Next generation sequencing; Sequenciamento genético; Platonia Insignis; Bacuri; Clusiaceae; Oligonucleotides.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121255
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Genetic diversity assessed by microsatellite markers in sweet corn cultivars Scientia Agricola
Lopes,Ana Daniela; Scapim,Carlos Alberto; Machado,Maria de Fátima Pires da Silva; Mangolin,Claudete Aparecida; Silva,Tereza Aparecida; Cantagali,Liriana Belizário; Teixeira,Flávia França; Mora,Freddy.
Information on genetic diversity is essential to the characterization and utilization of germplasm. The genetic diversity of twenty-two sweet corn cultivars (seventeen open-pollinated varieties, OPV, and five hybrids, H) was investigated by applying simple sequence repeat markers. A total of 257 primers were tested, of which 160 were found to be usable in terms of high reproducibility for all the samples tested; 45 were polymorphic loci, of which 30 were used to assess the genetic diversity of sweet corn cultivars. We detected a total of 86 alleles using 30 microsatellite primers. The mean polymorphism was 82 %. The highest heterozygosity values (Ho = 0.20) were found in the PR030-Doce Flor da Serra and BR427 III OPVs, whereas the lowest values (0.14) were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Simple sequence repeats; Neighbor-joining; Maize germplasm.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000600513
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Identification of a minimal microsatellite marker panel for the fingerprinting of peach and nectarine cultivars Electron. J. Biotechnol.
Rojas,Gabriela; Méndez,Marco A; Muñoz,Carlos; Lemus,Gamalier; Hinrichsen,Patricio.
The genetic characterization of 117 peach and nectarine cultivars (Prunus persica (L.) Batsch) using microsatellite (SSR) markers is presented. Analyzed genotypes include the complete list of cultivars under intellectual property (IP) protection in Chile. One hundred and two out of the 117 cultivars under study could be identified using only 7 SSRs. Other 5 cultivars were differentiated using 3 additional markers, but 5 pairs of genotypes were not differentiable. The average expected heterozygosity for the set of markers was 0.55, ranging from 0.28 in BPPCT-008 to 0.81 in CPPCT-022, with an F value of 0.37. A Neighbor-Joining dendrogram showed that, with few exceptions, peaches and nectarines clustered separately. These results are the basis for the...
Tipo: Journal article Palavras-chave: Cultivar identification; Fingerprinting; Prunus persica; Simple sequence repeats; SSR.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500004
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Methodology of tetraploid induction plants and expression of microsatellite alleles in triploid watermelon National Institute of Agronomic Research
Omran, S.A.; Guerra-Sanz, J.M.; Garrido Cárdenas, J.A..
Methods to obtain tetraploid watermelon plants from germinating seeds by treatments with chromosome doubling chemicals have been assayed and some tetraploid plants obtained by such treatments were pollinated with a diploid to obtain triploid watermelons. Three concentrations each of dinitroaniline (12 to 15 mg.L-1) and colchicine (300 to 500 mg.L-1), using three exposure durations (12 to 36 h), were compared for efficiency of tetraploid induction on germinating seeds of five watermelon cultivars. In all five cultivars, the highest percentage of tetraploid plants was obtained using dinitroaniline at concentration of 12 mg.L-1 for 24 hrs. The expression of the microsatellite alleles in a triploid obtained from crossing a tetraploid plant of ‘Sugar Baby’ with...
Tipo: Poster Palavras-chave: Citrullus lanatus; Polyploidy; Dinitroaniline; Colchicine; Simple sequence repeats; Watermelon.
Ano: 2008 URL: http://hdl.handle.net/2174/235
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Otimização da utilização de marcadores moleculares microssatélites e sua aplicação em estudos com plantas daninhas Planta Daninha
Goulart,I.C.G.R; Merotto Junior,A; Nunes,A.L; Bered,F.
Apesar de existirem marcadores moleculares mais específicos, os marcadores microssatélites apresentam grande potencialidade de utilização na área de plantas daninhas devido à sua crescente disponibilização em outras espécies e à qualidade das informações proporcionadas. O uso convencional dos marcadores moleculares microssatélites demanda grande quantidade de trabalho e recursos financeiros. O objetivo deste trabalho foi descrever a técnica da cauda fluorescente como forma de otimização da utilização de marcadores moleculares microssatélites, utilizando como exemplo um estudo de identificação de híbridos entre arroz-vermelho e cultivado. Foram utilizadas como modelo plantas de arroz cultivado, arroz-vermelho e o híbrido originado do cruzamento artificial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arroz-vermelho; Cauda M13; Hibridização; Primer marcado; Simple sequence repeats; SSR.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582011000500025
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Plant microsatellite genotyping with 4-color fluorescent detection using multiple-tailed primers. Repositório Alice
MISSIAGGIA, A.; GRATTAPAGLIA, D..
Tipo: Separatas Palavras-chave: Multiplex polymerase chain reaction; Universal primers; Simple sequence repeats.
Ano: 2006 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/186742
Registros recuperados: 9
Primeira ... 1 ... Última
 

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