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Registros recuperados: 43 | |
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Coutinho,E.L.; Gomes,A.R.S.; França,C.N.; Salvini,T.F.. |
An alternative device for the immobilization of the hind limb of the rat was developed to study the effects of chronic disuse on the soleus and tibialis anterior muscles, maintained for 3 weeks in the shortening and the stretching positions, respectively. The proposed device is made of steel mesh and cotton materials, and has some advantages when compared to cast or plaster cast: it is cheaper, lighter (12 g or 4% of the body weight of the rat) and the same unit can be easily adjusted and used several times in the same animal or in animals of similar size. Immobilization is also useful to restrain the movements of the hip, knee, and ankle joints. Male rats (291 ± 35 g and aged 14 ± 2 weeks) were used to develop and test the model. The soleus muscle of 18... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Muscle immobilization; Muscle shortening; Muscle stretching; Soleus muscle; Tibialis anterior; Skeletal muscle. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100010 |
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Baldo,C; Ferreira,MJ; Lopes,DS; Izidoro,LFM; Gomes,AO; Ferro,EAV; Hamaguchi,A; Homsi-Brandeburgo,MI; Rodrigues,VM. |
The damaging effects of neuwiedase, a non-hemorrhagic snake venom metalloproteinase from P-I class, on gastrocnemius muscle are studied herein. Following neuwiedase injection, ultrastructural alterations were detected early showing disarrangement of skeletal muscle fibers (characterized by discontinuity of Z lines), mitochondrial swelling, and disruption of plasma membrane and basal lamina. Degradation of skeletal muscle and the appearance of an amorphous substance, primarily composed of cellular debris, were noted after 24 hours. The presence of neuwiedase at the injection site (detected by immunocytochemistry) revealed highly specific labeling of myofibril components of damaged myocytes. In addition, proteolysis of muscle proteins assayed through... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Skeletal muscle; Neuwiedase; Snake venom metalloproteinases; Bothrops neuwiedi. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300013 |
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Mesquita-Ferrari,RA; Moraes,CK de; Micocci,KC; Selistre-de-Araújo,HS. |
ALT-C, an ECD motif (glutamic acid, cysteine, aspartic acid) disintegrin from Bothrops alternatus snake venom, induces α2β1 integrin-mediated signaling and neutrophil chemotaxis. In vitro, in human umbilical vein endothelial cells (HUVEC), ALT-C induces cell proliferation, thus showing an interesting potential for tissue regeneration studies. This work aimed to evaluate the influence of ALT-C in myoblast viability and differentiation. Myoblasts were obtained from hind limb muscles of 3 to 4-day old Wistar rats. The cells were incubated with ALT-C at different concentrations and incubation periods were followed by total RNA isolation. cDNA synthesis and real time polymerase chain reaction (PCR) were performed with primers of myoD as well as of both (slow... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Viability; Myosin heavy chain; Myoblast; Disintegrin; Skeletal muscle; Bothrops alternatus. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200013 |
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OLIVEIRA, P. S. N. de; COUTINHO, L. L.; TIZIOTO, P. C.; CESAR, A. S. M.; OLIVEIRA, G. B. de; DINIZ, W, J. da S.; LIMA, A. O. de; REECY, J. M.; MOURÃO, G. B.; ZERLOTINI NETO, A.; REGITANO, L. C. de A.. |
Residual Feed Intake (RFI) is an economically relevant trait in beef cattle. Among the molecular regulatory mechanisms, microRNAs (miRNAs) are an important dimension in post-transcriptional regulation and have been associated with different biological pathways. Here, we performed differential miRNAs expression and weighted gene co-expression network analyses (WGCNA) to better understand the complex interactions between miRNAs and mRNAs expressed in bovine skeletal muscle and liver. MiRNA and mRNA expression data were obtained from Nelore steers that were genetically divergent for RFI (N = 10 [low RFI or feed efficient]; N = 10 [high RFI or feed inefficient]). Differentially expressed and hub miRNAs such as bta-miR-486, bta-miR-7, bta-miR15a, bta-miR-21,... |
Tipo: Artigo de periódico |
Palavras-chave: RFI; MiRNA; Gado Nelore; Beef cattle; Nellore; Skeletal muscle. |
Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1099755 |
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OLIVEIRA, P. S. N. de; COUTINHO, L. L.; TIZIOTO, P. C.; CESAR, A. S. M.; OLIVEIRA, G. B. de; DINIZ, W, J. da S.; LIMA, A. O. de; REECY, J. M.; MOURÃO, G. B.; ZERLOTINI NETO, A.; REGITANO, L. C. de A.. |
Residual Feed Intake (RFI) is an economically relevant trait in beef cattle. Among the molecular regulatory mechanisms, microRNAs (miRNAs) are an important dimension in post-transcriptional regulation and have been associated with different biological pathways. Here, we performed differential miRNAs expression and weighted gene co-expression network analyses (WGCNA) to better understand the complex interactions between miRNAs and mRNAs expressed in bovine skeletal muscle and liver. MiRNA and mRNA expression data were obtained from Nelore steers that were genetically divergent for RFI (N = 10 [low RFI or feed efficient]; N = 10 [high RFI or feed inefficient]). Differentially expressed and hub miRNAs such as bta-miR-486, bta-miR-7, bta-miR15a, bta-miR-21,... |
Tipo: Artigo de periódico |
Palavras-chave: MicroRNAs; Residual Feed Intake; Gado Nelore; Beef cattle; Nellore; Skeletal muscle. |
Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102149 |
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Davatz,Giovanna Castilho; Andreo,Jesus Carlos; Rodrigues,Antonio de Castro; Rosa Júnior,Geraldo Marco; Moraes,Luiz Henrique Rapucci. |
The apoptosis phenomenon happens in normal tissues and in many pathological conditions as described by several researchers. Most studied in the 70s, this phenomenon remained almost forgotten in the 90s, when, with the advent of more advanced techniques, returned to laboratories. Thus, electronic microscopy, Comet Assays and histochemistry techniques, among others, were utilized in an effort to elucidate in a thorough way, the mechanisms that lead the cell to "suicide". No one can deny the importance of the knowledge of such phenomenon, so as to control its induction or blocking in the treatment of pathologies such as cancer. In this work, the apoptosis study was aimed at denervated muscles in different periods. Furthermore, two types of skeletal striated... |
Tipo: Journal article |
Palavras-chave: Skeletal muscle; Denervation; Apoptosis; Rat. |
Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300009 |
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RAMIREZ,BEATRIZ U; RETAMAL,LEIRA; VERGARA,CECILIA. |
The well-established trophic role of CNTF upon neurons led to performing clinical trials in patients of neurodegenerative diseases. However, trials were suspended due to side effects such as severe weight loss, hyperalgesia, coughing, muscle cramps and pain. So far it is not known how CNTF triggers the problems related to skeletal muscle cramps and pain. CNTF has also been described as a myotrophic factor for denervated skeletal muscles, but the possibility that it affects innervated muscles has also been considered. Since a myotrophic factor could be a valuable tool for treatment of several muscle diseases, we studied the effects of low doses of CNTF delivered systemically by an osmotic pump, over the electrical and mechanical properties of innervated and... |
Tipo: Journal article |
Palavras-chave: CNTF; Contraction and relaxation time; Denervation; EMG; Fibrillation; Skeletal muscle. |
Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300003 |
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Fukushima,M.G.; Furlan,I.; Chiavegatti,T.; Kiyomoto,B.H.; Godinho,R.O.. |
The present study analyzes the ectopic development of the rat skeletal muscle originated from transplanted satellite cells. Satellite cells (10(6) cells) obtained from hindlimb muscles of newborn female 2BAW Wistar rats were injected subcutaneously into the dorsal area of adult male rats. After 3, 7, and 14 days, the transplanted tissues (N = 4-5) were processed for histochemical analysis of peripheral nerves, inactive X-chromosome and acetylcholinesterase. Nicotinic acetylcholine receptors (nAChRs) were also labeled with tetramethylrhodamine-labeled alpha-bungarotoxin. The development of ectopic muscles was successful in 86% of the implantation sites. By day 3, the transplanted cells were organized as multinucleated fibers containing multiple clusters of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Skeletal muscle; Satellite cells; Stem cells; Transplantation; Myogenesis; 2BAW rats. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300007 |
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Abreu,P.; Vitzel,K.F.; Monteiro,I.C.C.R.; Lima,T.I.; Queiroz,A.N.; Leal-Cardoso,J.H.; Hirabara,S.M.; Ceccatto,V.M.. |
The aim of this research was to investigate the effects of endurance training on reduction of plasma glucose during high intensity constant and incremental speed tests in Wistar rats. We hypothesized that plasma glucose might be decreased in the exercised group during heavy (more intense) exercise. Twenty-four 10-week-old male Wistar rats were randomly assigned to sedentary and exercised groups. The prescription of endurance exercise training intensity was determined as 60% of the maximum intensity reached at the incremental speed test. The animals were trained by running on a motorized treadmill, five days/week for a total period of 67 weeks. Plasma glucose during the constant speed test in the exercised group at 20 m/min was reduced at the 14th, 21st and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Exercise; Skeletal muscle; Liver; Blood glucose; Training. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001100602 |
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Feder,D.; Rugollini,M.; Santomauro Jr,A.; Oliveira,L.P.; Lioi,V.P.; dos Santos,R.; Ferreira,L.G.; Nunes,M.T.; Carvalho,M.H.; Delgado,P.O.; Carvalho,A.A.S.; Fonseca,F.L.A.. |
Erythropoietin (EPO) has been well characterized as a renal glycoprotein hormone regulating red blood cell production by inhibiting apoptosis of erythrocyte progenitors in hematopoietic tissues. EPO exerts regulatory effects in cardiac and skeletal muscles. Duchenne muscular dystrophy is a lethal degenerative disorder of skeletal and cardiac muscle. In this study, we tested the possible therapeutic beneficial effect of recombinant EPO (rhEPO) in dystrophic muscles in mdx mice. Total strength was measured using a force transducer coupled to a computer. Gene expression for myostatin, transforming growth factor-β1 (TGF-β1), and tumor necrosis factor-α (TNF-α) was determined by quantitative real time polymerase chain reaction. Myostatin expression was... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Muscular dystrophy; Erythropoietin; Myostatin; Skeletal muscle; Quadriceps. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001100966 |
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TIIDUS,PETER M; DELLER,MIRADA; BOMBARDIER,ERIC; GÜL,MUSTAFA; LIU,X. LINDA. |
This study examined the effects of estrogen supplementation on markers of neutrophil infiltration and damage in skeletal muscle of rats following ischemia. Male and female gonad-intact rats, with or without 14 days of estrogen supplementation were subjected to two hours of hind-limb ischemia and sacrificed at 24, 48 or 72 hours post-ischemia. Control animals were sacrificed without ischemia. Plantaris and red and white gastrocneimus muscles were removed and assayed for myeloperoxidase (MPO), a marker of neutrophil infiltration, and glucose-6-phosphate dehydrogenase (G6PD) and ß-glucuronidase (<img border=0 width=8 height=15 id="_x0000_i1026" src="http://fbpe/img/bres/v38n2-3/existe.gif" align=absbottom>GLU), as markers of muscle damage. Significant... |
Tipo: Journal article |
Palavras-chave: Ischemia; Estrogen; Muscle damage; Skeletal muscle; Neutrophils. |
Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200011 |
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Registros recuperados: 43 | |
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