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Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide BJMBR
Goldstein,J.; Silberstein,C.; Ibarra,C..
Adenylyl cyclase (AC) isoforms catalyze the synthesis of 3',5'-cyclic AMP from ATP. These isoforms are critically involved in the regulation of gene transcription, metabolism, and ion channel activity among others. Nitric oxide (NO) is a gaseous product whose synthesis from L-arginine is catalyzed by the enzyme NO synthase. It has been well established that NO activates the enzyme guanylyl cyclase, but little has been reported on the effects of NO on other important second messengers, such as AC. In the present study, the effects of sodium nitroprusside (SNP), a nitric oxide-releasing compound, on COS-7 cells transfected with plasmids containing AC types I, II, V and VI were evaluated. Total inhibition (~98.5%) of cAMP production was observed in COS-7...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adenylyl cyclase; Nitric oxide; Sodium nitroprusside; Cyclic AMP; COS-7 cells; Signal transduction.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200002
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Comparison of different methods of goat sperm selection and capacitation for optimization of assisted reproductive technologies. Repositório Alice
OLIVARES, C. C. S.; FONSECA, J. F. da; CAMARGO, L. S. de A.; FABJAN, J. M. G. de S.; RODRIGUES, A. L. R.; BRANDÃO, F. Z..
This study evaluated different methods of frozen-thawed sperm selection and capacita-tion in goats for further use in reproductive biotechnologies. In Experiment 1, semen wasprocessed by the following techniques: mini-Percoll, swim-up, or washing by centrifuga-tion. In Experiment 2, mini-Percoll selected-sperm was subjected to capacitation inductionby incubation with: 50 ug/mL heparin, 10 uM, 50 uM, or 100 uM of sodium nitroprusside(SNP). Motility, vigor, acrosome, and plasma membrane (PM) integrity were evaluated afterthawing and after each treatment of sperm selection or capacitation. In Experiment 1, wash-ing by centrifugation presented greater (43%; P < 0.05) spermatozoa recovery rate than theother treatments. The swim-up technique showed the lowest...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Capacitation; Sodium nitroprusside; Sperm preparation; Sperm selection; Goats; Reproduction; Spermatozoa; Caprino; Espermatozóide; Reprodução animal; Seleção; Sêmen.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1035586
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Electrolyte Leakage and the Protective Effect of Nitric Oxide on Leaves of Flooded Rice Exposed to Herbicides Planta Daninha
SILVA,K.S.; URBAN,L.J. K.; BALBINOT,A.; GNOCATO,F.S.; KRUSE,N.D.; MARCHESAN,E.; MACHADO,S.L.O..
ABSTRACT The nitric oxide acts on the antioxidant system of plants and can discontinue the damage of herbicides elicitors of oxidative stress that cause the disruption of membranes and leakage of cellular contents. In order to evaluate the protective effect of nitric oxide in electrolytes leakage, leaf segments of the Puita INTA CL rice cultivar were incubated with 0, 5, 50, 500 and 5,000 μM clomazone (360 g a.i. L-1), oxadiazon (250 g a.i. L-1), oxyfluorfen (240 g a.i. L-1) and the formulated mixture of paraquat (200 g a.i. L-1) + diuron (100 g a.i. L-1) to obtain the maximum potential conductivity of 50% (MPC50). Subsequently, leaf segments were pre-treated with 0, 200 and 2,000 μM of sodium nitroprusside (SNP) for four hours and further incubated for 48...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oryza sativa; Sodium nitroprusside; Clomazone; Oxadiazon; Oxyfluorfen; Paraquat + diuron.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000400777
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Nitric oxide induced alleviation of toxic effects of short term and long term Cd stress on growth, oxidative metabolism and Cd accumulation in Chickpea Braz. J. Plant Physiol.
Kumari,Anita; Sheokand,Sunita; Swaraj,Kumari.
The present study investigates the effect of long and short term Cd stress in chickpea plants and evaluates the protective effect of exogenous nitric oxide (NO) supplementation using sodium nitroprusside (SNP). Cadmium treatments were given before sowing (long term stress) and thirty days after germination (short term stress). Sodium nitroprusside was given as foliar spray 30 days after germination to both long and short term Cd treated plants. Cadmium adversely affected the membranes as was evident from increased electrolyte leakage and lipid peroxidation levels. Sodium nitroprusside treatments decreased ion leakage and lipid peroxidation levels significantly. Short term Cd stress resulted in a higher induction of the catalase, peroxidase, ascorbate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant enzymes; Lipid peroxidation; Membrane injury; Sodium nitroprusside.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000400007
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