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Liquid culture production of microsclerotia and submerged conidia by Trichoderma harzianum active against damping-off disease caused by Rhizoctonia solani. Repositório Alice
KOBORI, N. N.; MASCARIN, G. M.; JACKSON, M. A.; SCHISLER, D. A..
Media and culturing protocols were identified that supported the formation of submerged conidia and microsclerotia (MS) by Trichoderma harzianum Rifai strain T-22 using liquid culture fermentation. Liquid media with a higher carbon concentration (36 g L -1) promoted MS formation at all C:N ratios tested. Hyphae aggregated to form MS after 2 d growth and after 7 d MS were fully melanized. This is the first report of MS formation by T. harzianum or any species of Trichoderma. Furthermore, submerged conidia formation was induced by liquid culture media, but yields, desiccation tolerance, and storage stability varied with C:N ratio and carbon rate. Air-dried MS granules (<4 % moisture) retained excellent shelf life under cool and unrefrigerated storage...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Rhizoctonia solani; Trichoderma harzianum; Biopesticides; Soil-borne diseases; Sclerotia; Carbon nitrogen ratio.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1011851
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Survey of fungi associated with cassava root rot from different producing regions in Brazil Scientia Agricola
Boas,Sandielle Araújo Vilas; Oliveira,Saulo Alves Santos de; Bragança,Carlos Augusto Dórea; Ramos,Juliana Barros; Oliveira,Eder Jorge de.
ABSTRACT: Although root rot is one of the major diseases affecting Brazilian cassava (Manihot esculenta Crantz.), little is known about the diversity of root rot pathogens. In this study, diseased plants exhibiting root rot symptoms were collected from cassava-producing regions in five Brazilian states: Bahia, Sergipe, Paraíba, Maranhão, Tocantins, and Paraná. Seventy isolates were obtained and assigned to species complexes based on rDNA's ITS (internal transcribed spacer of the ribosomal DNA) region (ITS1, ITS2 and 5.8S). A total of seven species complexes was found belonging to the genus Fusarium (56/74), followed by Lasiodiplodia (8/74), Neoscytalidium (6/74), and Diaporthe/Phomopsis complex (2/74), Phytophthora, and Corallomycetella (1/74 each). These...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Manihot esculenta; ITS barcode; Phylogeny; Soil-borne diseases.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000100060
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