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| Registros recuperados: 14 | |
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| OLIVEIRA, K. C. de; GUIMARÃES, P. de S.; BAZIOLI, J. M.; MARTINATI, J. C.; SANTOS, M. M. dos; PADILHA, L.; GUERREIRO-FILHO, O.; MALUF, M. P.. |
| Cloning of superior coffee plants by somatic embryogenesis can assist breeding programs on reducing the cost and time for launch of new cultivars. This study aimed to evaluate the efficiency of this methodology for cloning coffee trees with high heterozygosity, and to gather evidence that clonal progenies are faithful copies of mother plants. Selected plants IAC1 and IAC 2 from Coffea arabica breeding populations, resistant to leaf rust and leaf miner, respectively, were cloned via indirect somatic embryogenesis. Expression of selected genes involved in biological processes potentially affected by in vitro cultivation was evaluated by quantitative analysis. Genes encoding proteins associated with maintenance of DNA integrity and control of cell cycle... |
| Tipo: Artigo de periódico |
Palavras-chave: In vitro cultivation; Differential expression; Coffea Arábica; Somaclonal variation. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113261 |
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| Baránek,Miroslav; Raddová,Jana; Krizan,Bretislav; Pidra,Miroslav. |
| The Amplification Fragment Length Polymorphism (AFLP) technique was employed to study genetic variations which can be induced in vines by the stress occurring during different aspects of viticulture (in vitro cultivation, in vitro thermotherapy and virus infection). Analysis of AFLP banding patterns, generated by using 15 primer combinations, pointed to negligible genetic variation among plants exposed to individual stress. The average of similarity coefficients between differently stressed plants of the cultivars Müller Thurgau and Riesling were 0.984 and 0.991, respectively, as revealed by AFLP analysis. The low incidence of observed polymorphism demonstrates the high level of genome uniformity in plants reproduced by in vitro micropropagation via nodes,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Grapevine; Stress; Somaclonal variation; Virus infection; AFLP. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400026 |
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| Zaffari,Gilmar Roberto; Peres,Lázaro Eustáquio Pereira; Tcacenco,Fernando Adami; Kerbauy,Gilberto Barbante. |
| Nanism is one of the most frequent type of mutant in micropropagated banana plants from the Cavendish subgroup. The present study aimed at studying some of the hormone factors involved in this type of mutation. Rhizomes from normal and dwarf plants from the cultivar Grand Naine were incubated for 5 d in the presence of [³H]-L-tryptophan, [³H]-indole-3-acetic acid and gibberellin, to quantify the endogenous levels of indole-3-acetic acid-ester, indole-3-acetic acid-amide, free indole-3-acetic acid, and cytokinins. The endogenous levels of indole-3-acetic acid and its ester- and amide-conjugated forms were measured in normal and dwarf plants incubated for 30 d with gibberellin, indole-3-acetic acid, and L- and D-tryptophan. In normal plants, the use of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Indole-3-acetic acid metabolism; Growth regulators; Somaclonal variation. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000300005 |
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| Lakshmanan,Venkatachalam; Reddampalli Venkataramareddy,Sreedhar; Neelwarne,Bhagyalakshmi. |
| A large number of micropropagated plantlets of banana, Musa acuminata var. Nanjanagudu Rasabale (NR), that were developed from axillary shoot bud explants over 10 years ago were screened for genetic variation, if any, using RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeats) markers. Of the 4000 in vitro plantlets, 11 were used for screening that involved shoot cultures with distinct variation in morphological characteristics (morphotypes). Similarly, the mother maintained in the field was also subjected for genetic analysis. Out of the 50 RAPD and 25 ISSR primers screened, 30 RAPD and 5 ISSR primers produced totally 424 clear, distinct and reproducible band classes resulting in a total of 5088 bands where the banding patterns... |
| Tipo: Journal article |
Palavras-chave: Growth regulators; Musa; Protocol; Shoot cultures; Somaclonal variation. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100010 |
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| Maraschin,M.; Sugui,J.A.; Wood,K.V.; Bonham,C.; Buchi,D.F.; Cantao,M.P.; Carobrez,S.G.; Araujo,P.S.; Peixoto,M.L.; Verpoorte,R.; Fontana,J.D.. |
| Cell cultures of Mandevilla velutina have proved to be an interesting production system for biomass and secondary metabolites able to inhibit the hypotensive activity of bradykinin, a nonapeptide generated in plasma during tissue trauma. The crude ethyl acetate extract of cultured cells contains about 31- to 79-fold more potent anti-bradykinin compounds (e.g., velutinol A) than that obtained with equivalent extracts of tubers. Somaclonal variation may be an explanation for the wide range of inhibitor activity found in the cell cultures. The heterogeneity concerning morphology, differentiation, carbon dissimilation, and velutinol A production in M. velutina cell cultures is reported. Cell cultures showed an asynchronous growth and cells in distinct... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mandevilla velutina; Secondary metabolism; Plant cell cultures; Somaclonal variation; Phytosteroid; Anti-bradykinin compounds. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600002 |
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| Registros recuperados: 14 | |
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