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| OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BALARO, M. F. A.; FREITAS, V. J. de F.; OLIVEIRA, R. V. de; BRANDÃO, F. Z.. |
| Resumo: Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evaluating the influence of different sperm selection techniques on ram sperm parameters in semen preparation. Materials, Methods & Results: Frozen-thawed commercial semen from 10 Santa Inês rams was subjected to either: swim-up, Percoll, mini-Percoll, sperm washing by centrifugation or a control group. After each technique, samples were incubated at 37°C for... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Sperm preparation; Sperm capacitation; CASA; CTC; Plasma membrane integrity; Protocolo; Ovino; Espermatozóide; Sêmen; Reprodução animal; Sheep; Reproduction; Reproductive techniques; Spermatozoa; Semen; Treaties. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1070178 |
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| Wang,Jiangtao; He,Qingliu; Yan,Xingyu; Cai,Youmei; Chen,Junyi. |
| BACKGROUND: Nitric oxide (NO) has been shown to be important in sperm function, and the concentration of NO appears to determine these effects. Studies have demonstrated both positive and negative effects of NO on sperm function, but have not been able to provide a clear link between NO concentration and the extent of exposure to NO. To study the relationship between nitric oxide and sperm capacitationin vitro, and to provide a theoretical basis for the use of NO-related preparations in improving sperm motility for in vitro fertilization, we investigated the effects of NO concentration and time duration at these concentrations on in vitro sperm capacitation in both normal and abnormal sperm groups. We manipulated NO concentrations and the time duration of... |
| Tipo: Journal article |
Palavras-chave: Nitric oxide; Sperm capacitation; Sperm motility; Time; Human. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100044 |
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| Wu,Shao-Chin; Liu,Min. |
| Cigarette smoke is known to be a serious health risk factor and considered reproductively toxic. In the current study, we investigated whether constituents of cigarette smoke, pyrazine, 2-ethylpyridine, and 3-ethylpyridine, adversely affect reproductive functioning such as oocyte maturation and sperm capacitation. Our findings indicated that three smoke components were involved in retardation of oocyte maturation in a dose-dependent manner and the lowest-observed-adverse-effect level (LOAEL) was determined to be 10-10M. However, individual smoke components administrated at the LOAEL did not attenuate oocyte maturation, demonstrating that all three toxicants were equally required for the observed growth impairment. When exposed to all three components at... |
| Tipo: Journal article |
Palavras-chave: Cigarette smoke; Oocyte maturation; Reproductive toxicity; Sperm capacitation. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200001 |
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| SANTOS, A. X.; BRITO, V. C.; GONÇALVES, A. A.; RAMOS, P. C. A.; ROLIM FILHO, S. T.; RIBEIRO, H. F. L.; MIRANDA, M. S.; SANTOS, S.S. D.; GARCIA, A. R.; OHASHI, O. M.. |
| 2015 |
| Tipo: Resumo em anais de congresso (ALICE) |
Palavras-chave: Buffalo embryo; Cyclodextrin; Sperm capacitation. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1023894 |
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| Olivares,C.C.S.; Souza-Fabjan,J.M.G.; Fonseca,J.F.; Saraiva,H.F.R.A.; Côrtes,L.R.; Alfradique,V.A.P.; Balaro,M.F.A.; Oliveira,R.V.; Brandão,F.Z.. |
| ABSTRACT This study evaluated the effect of increasing centrifugal force and reducing centrifugation time and volume in Percoll protocols on ram sperm parameters. Commercial semen of Santa Inês rams were used and five treatments were performed: traditional Percoll and mini-Percoll (MP) techniques (I- 5000 x g, 5min; II- 2500 x g, 5min; III- 1250 x g, 5min; IV- 700 x g, 10min). At post-thawing (PT) and post-selection protocols (0h), samples were assessed for spermatozoa recovery rate, motility, plasma membrane (PM) integrity, sperm capacitation and morphology and incubated at 37 C for 1, 2 and 3h. The sperm recovery rate averaged 9.1±1.4%, and most motility parameters were similar (P> 0.05) among protocols. VCL (µm/s) was higher (P< 0.05) after MP-II,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sperm capacitation; Frozen-thawed sperm; Ovine; Percoll protocols. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200455 |
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| OLIVEIRA, C. C. S.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; ALFRADIQUE, V. A. P.; CÔRTES, L. R.; BRANDÃO, F. Z.. |
| Sperm capacitation is a prerequisite for mammal successful fertilization. Although usually a capacitating substance such as heparin is used during sheep in vitro fertilization, evidences suggest that the cryopreservation process and Percoll technique could induce spontaneous capacitation. This study aimed to compare ovine, caprine and bovine frozen-thawed sperm after mini-Percoll processing on sperm parameters, receiving or not heparin supplementation. In conclusion, frozen-thawed ovine, caprine and bovine spermatozoa processed with mini-Percoll behave similarly regarding to capacitation status and does not require heparin supplementation during in vitro incubation to achieve capacitation. [Processamento pela técnica de mini-Percoll em sêmen... |
| Tipo: Resumo em anais de congresso (ALICE) |
Palavras-chave: Sperm capacitation; Capacitação espermática; Ovino; Caprino; Bovino; Reprodução animal; Sêmen; Heparina; Sheep; Goats; Cattle; Spermatozoa; Reproduction; In vitro fertilization; Heparin. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080820 |
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| ALFRADIQUE, V. A. P.; OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; CÔRTES, L. R.; BRANDÃO, F. Z.. |
| Background: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted by the female reproductive tract epithelium, heparin promotes capacitation by binding to and removing seminal plasma proteins, which are adsorbed to the sperm PM and would inhibit capacitation. There is substantial evidence that cryopreservation promotes capacitation-like changes in bull, ram and buck sperm. Our general hypotheses were: (a) cryopreserved ram sperm suffer capacitation more quickly than buck and bull sperm under the same conditions; (b) the capacitation status of ruminant cryopreserved sperm is similar whether or not heparin is present after... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Billygoats; CTC; Caprino; Ovino; Bovino; Bode; Carneiro; Reprodução Animal; Sêmen; Heparina; Espermatozóide; Sheep; Goats; Cattle; Rams; Bulls; Animal reproduction; Sperm capacitation; Assisted reproductive technologies; Heparin. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1119218 |
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