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| OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BALARO, M. F. A.; FREITAS, V. J. de F.; OLIVEIRA, R. V. de; BRANDÃO, F. Z.. |
| Resumo: Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evaluating the influence of different sperm selection techniques on ram sperm parameters in semen preparation. Materials, Methods & Results: Frozen-thawed commercial semen from 10 Santa Inês rams was subjected to either: swim-up, Percoll, mini-Percoll, sperm washing by centrifugation or a control group. After each technique, samples were incubated at 37°C for... |
| Tipo: Artigo de periódico |
Palavras-chave: Sperm preparation; CASA; CTC; Plasma membrane integrity; Protocolo; Reproductive techniques.; Ovino; Espermatozóide; Sêmen; Reprodução animal; Sheep; Reproduction; Spermatozoa; Treaties; Sperm capacitation.. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1070178 |
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| SANTOS, A. X.; BRITO, V. C.; GONÇALVES, A. A.; RAMOS, P. C. A.; ROLIM FILHO, S. T.; RIBEIRO, H. F. L.; MIRANDA, M. S.; SANTOS, S. S. D.; GARCIA, A. R.; OHASHI, O. M.. |
| Publicado: Proceedings of the 29th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Gramado, RS, Brazil, August 20th to 23rd, 2015, and 31st Meeting of the European Embryo Transfer Association (AETE); Ghent, Belgium, September 11th and 12th, 2015. Abstracts. |
| Tipo: Separatas |
Palavras-chave: Buffalo embryo; Cyclodextrin.; Sperm capacitation.. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1023894 |
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| OLIVEIRA, C. C. S.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; ALFRADIQUE, V. A. P.; CÔRTES, L. R.; BRANDÃO, F. Z.. |
| Sperm capacitation is a prerequisite for mammal successful fertilization. Although usually a capacitating substance such as heparin is used during sheep in vitro fertilization, evidences suggest that the cryopreservation process and Percoll technique could induce spontaneous capacitation. This study aimed to compare ovine, caprine and bovine frozen-thawed sperm after mini-Percoll processing on sperm parameters, receiving or not heparin supplementation. In conclusion, frozen-thawed ovine, caprine and bovine spermatozoa processed with mini-Percoll behave similarly regarding to capacitation status and does not require heparin supplementation during in vitro incubation to achieve capacitation. [Processamento pela técnica de mini-Percoll em sêmen... |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: Capacitação espermática.; Ovino; Caprino; Bovino; Reprodução animal; Sêmen; Heparina; Sheep; Goats; Cattle; Spermatozoa; Reproduction; In vitro fertilization; Heparin; Sperm capacitation.. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080820 |
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