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Comparison of different methods of goat sperm selection and capacitation for optimization of assisted reproductive technologies. Repositório Alice
OLIVARES, C. C. S.; FONSECA, J. F. da; CAMARGO, L. S. de A.; FABJAN, J. M. G. de S.; RODRIGUES, A. L. R.; BRANDÃO, F. Z..
This study evaluated different methods of frozen-thawed sperm selection and capacita-tion in goats for further use in reproductive biotechnologies. In Experiment 1, semen wasprocessed by the following techniques: mini-Percoll, swim-up, or washing by centrifuga-tion. In Experiment 2, mini-Percoll selected-sperm was subjected to capacitation inductionby incubation with: 50 ug/mL heparin, 10 uM, 50 uM, or 100 uM of sodium nitroprusside(SNP). Motility, vigor, acrosome, and plasma membrane (PM) integrity were evaluated afterthawing and after each treatment of sperm selection or capacitation. In Experiment 1, wash-ing by centrifugation presented greater (43%; P < 0.05) spermatozoa recovery rate than theother treatments. The swim-up technique showed the lowest...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Capacitation; Sodium nitroprusside; Sperm preparation; Sperm selection; Goats; Reproduction; Spermatozoa; Caprino; Espermatozóide; Reprodução animal; Seleção; Sêmen.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1035586
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Comparison of different sperm selection techniques in ram frozen-thawed sperm. Repositório Alice
OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BALARO, M. F. A.; FREITAS, V. J. de F.; OLIVEIRA, R. V. de; BRANDÃO, F. Z..
Resumo: Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evaluating the influence of different sperm selection techniques on ram sperm parameters in semen preparation. Materials, Methods & Results: Frozen-thawed commercial semen from 10 Santa Inês rams was subjected to either: swim-up, Percoll, mini-Percoll, sperm washing by centrifugation or a control group. After each technique, samples were incubated at 37°C for...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Sperm preparation; Sperm capacitation; CASA; CTC; Plasma membrane integrity; Protocolo; Ovino; Espermatozóide; Sêmen; Reprodução animal; Sheep; Reproduction; Reproductive techniques; Spermatozoa; Semen; Treaties.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1070178
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Influence of mini-Percoll techniques in sperm capacitation and plasma membrane integrity of ram frozen-thawed sperm. Repositório Alice
OLIVARES, C. C. S.; ALFRADIQUE, V. A. P.; FABJAN, J. M. G. S.; FONSECA, J. F. da; SARAIVA, H. F. R. A.; CÔRTES, L. R.; COSTA, L. C. da; BRANDÃO, F. Z..
2016
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Sperm selection; Sperm preparation; Mini-Percoll; Ovino; Ovelha; Reprodução animal; Espermatozóide; Sêmen; Sheep; Spermatozoa.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1052416
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