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An integrative view to structure, function, ontogeny and phylogenetical significance of the male genital system in Pomacea canaliculata (Caenogastropoda, Ampullariidae) Biocell
Gamarra-Luques,C.; Winik,B.C.; Vega,I.A.; Albrecht,E.A.; Catalan,N.M.; Castro-Vazquez,A..
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spermatogenesis; Steroids; Peptides; Imposex; Xenobiotics.
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200012
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Analysis of the black-chinned tilapia Sarotherodon melanotheron heudelotii reproducing under a wide range of salinities: from RNA-seq to candidate genes ArchiMer
Avarre, Jean-christophe; Dugue, R.; Alonso, Pascal; Diombokho, A.; Joffrois, C.; Faivre, N.; Cochet, C.; Durand, Jean Dominique.
The black-chinned tilapia Sarotherodon melanotheron heudelotii is an ecologically appealing model as it shows exceptional adaptive capacities, especially with regard to salinity. In spite of this, this species is devoid of genomic resources, which impedes the understanding of such remarkable features. De novo assembly of transcript sequences produced by next-generation sequencing technologies offers a rapid approach to obtain expressed gene sequences for non-model organisms. It also facilitates the development of quantitative real-time PCR (qPCR) assays for analysing gene expression under different environmental conditions. Nevertheless, obtaining accurate and reliable qPCR results from such data requires a number of validations prior to interpretation....
Tipo: Text Palavras-chave: Gene expression; Non-model organism; Reference gene; Salinity; Spermatogenesis; Tilapia.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00171/28255/26625.pdf
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Analysis of topological organization of chromatin during spermatogenesis in mouse testis Genet. Mol. Biol.
Narayan,Gopeshwar; Raman,Rajiva.
Eukaryotic chromatin is organized as radial DNA loops with periodical attachments to an underlying nucleoskeleton known as nuclear matrix. This higher order chromatin organization is revealed upon high salt extraction of cells. To understand the sequential change in the functional organization of chromatin during spermatogenesis, we have analysed the higher order organization of chromatin in different testicular cell types and the epididymal sperm of laboratory mouse. The expansion and contraction of the nucleoid DNA following 2 M NaCl extraction was measured in a fluorescence microscope using ethidium bromide (2.5-200 mg/mL) as an intercalating dye to induce DNA positive supercoils. While the halo size varied among cell types (pachytene DNA most extended,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromatin; DNase I; Nucleoid; Spermatogenesis.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100006
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Angiotensin-converting enzyme inhibitors of Bothrops jararaca snake venom affect the structure of mice seminiferous epithelium J. Venom. Anim. Toxins incl. Trop. Dis.
Alberto-Silva,Carlos; Gilio,Joyce M; Portaro,Fernanda C. V.; Querobino,Samyr M.; Camargo,Antonio C. M..
Background Considering the similarity between the testis-specific isoform of angiotensin-converting enzyme and the C-terminal catalytic domain of somatic ACE as well as the structural and functional variability of its natural inhibitors, known as bradykinin-potentiating peptides (BPPs), the effects of different synthetic peptides, BPP-10c (<ENWPHQIPP), BPP-11e (<EARPPHPPIPP), BPP-AP (<EARPPHPPIPPAP) and captopril were evaluated in the seminiferous epithelium of male mice.Methods The adult animals received either one of the synthetic peptides or captopril (120 nmol/dose per testis) via injection into the testicular parenchyma. After seven days, the mice were sacrificed, and the testes were collected for histopathological evaluation.Results BPP-10c...
Tipo: Info:eu-repo/semantics/article Palavras-chave: B. jararaca; Bradykinin-potentiating peptides; Angiotensin-converting enzyme; Seminiferous epithelium; Spermatogenesis.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100338
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Annual male reproductive activity and stages of the seminiferous epithelium cycle of the large fruit-eating Artibeus lituratus (Chiroptera: Phyllostomidae) Rev. Bras. Zool.
Notini,Alice A.; Farias,Talita O.; Talamoni,Sônia A.; Godinho,Hugo P..
The large fruit-eating phyllostomid bat, Artibeus lituratus (Olfers, 1818), forearm 69-75 mm, body mass 66-82 g, has a diversified geographic distribution in the Neotropical region. Therefore it is subjected to different climatic conditions that affect its reproduction, leading to different reproductive strategies such as continuous reproduction, seasonal monoestry or seasonal bimodal polyestry. In this study we used morphometric and histological methods to analyze the annual reproductive activity of A. lituratus males in a population living in the Atlantic Forest, Southeastern Brazil. Testis mass, epididymis mass, gonadosomatic index, seminiferous tubule diameter, and Leydig cell nucleus diameter showed no significant differences (p > 0.05) in the two...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Morphometry; Neotropical bat; Seminiferous epithelium; Spermatogenesis; Reproduction.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702015000300195
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Association of the infusion of Heteropterys aphrodisiaca and endurance training brings spermatogenetic advantages Biol. Res.
Gomes,Marcos L M; Monteiro,Juliana C; Freitas,Karine M; Sbervelheri,Mariana M; Dolder,Heidi.
The species Heteropterys aphrodisiaca is commonly used as a stimulant by popular medicine in the Cerrado, a savanna-like biome, Brazil. Recent studies have proved its protective effects on testes of animals submitted to treatment using Cyclosporine A, as well as its stimulus effect in increasing testosterone secretion. Therefore, the present study was designed to analyze whether the association of the plant infusion and endurance exercise could potentiate the stimulating effect. The animals were separated into 4 groups: two control (sedentary and trained) receiving water and two treated (sedentary and trained) receiving the plant infusion daily (104mg/day). The proportion of the seminiferous tubule compartment and interstitium was analyzed. Within the...
Tipo: Journal article Palavras-chave: Germ cells; Phytotherapy; Seminiferous epithelium; Spermatogenesis; Testosterone.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300004
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Cat, cougar, and jaguar spermatogenesis: a comparative analysis BABT
Costa,Deiler Sampaio; Paula,Tarcízio Antônio Rêgo; Matta,Sérgio Luiz Pinto da.
This work presents a comparative review about the spermatogenic process in cats (Felis domestica), jaguars (Panthera onca) and cougars (Puma concolor), with emphasis on testicular biometry, gonadossomatic index, volumetric proportion of testicular parenchyma components, tubular diameter, seminiferous epithelial height and seminiferous tubule length. It was an approach of the differences among the cell proportions that allowed conclusions about the overall yield of spermatogenic process and Sertoli cell index in three feline species.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spermatogenesis; Felines; Testis.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600006
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Chromosome complement and meiosis in three species of the Neotropical bug genus Antiteuchus (Heteroptera, Pentatomidae, Discocephalinae) Genet. Mol. Biol.
Lanzone,Cecilia; Souza,Maria José de.
Orcein staining of spermatocytes was used to study the meiotic behavior of holocentric chromosomes in three member of the genus Antiteuchus (commonly known as stink bugs). We describe and illustrate the karyotype of Antiteuchus mixtus, A. sepulcralis and A. macraspis which were cytogenetically characterized as having a diploid number of 2n = 14 and an XY sex chromosome system showing pre-reductional meiosis for autosomes and post-reductional meiosis for sex chromosomes. These species were also shown to have a long diffuse stage during meiotic prophase I and aberrant harlequin-type meiocytes. The chiasma frequency was also analyzed for two of the three species studied.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spermatogenesis; Holokinetic chromosomes; Sex chromosomes; Meiosis; Diffuse stage.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100011
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Contribution of environmental pollutants to male infertily: A working model of germ cell apoptosis induced by plasticizers Biol. Res.
Lagos-Cabré,Raúl; Moreno,Ricardo D.
Bisphenol A [2,2-bis(4-hydroxyphenyl)propane] (BPA), 4-nonylphenol (NP) and di(2-ethylhexyl)phthalate (DEHP), and its metabolite mono-2-ethylhexyl phthalate (MEHP) are chemicals found in plastics, which act as endocrine disruptors (EDs) in animals, including human. EDs act like hormones in the endocrine system, and disrupt the physiologic function of endogenous hormones. Most people are exposed to different endocrine disruptors and concern has been raised about their true effect on reproductive organs. In the testis, they seem to preferentially attack developing testis during puberty rather than adult organs. However, the lack of information about the molecular mechanism, and the apparently controversial effect observed in different models has hampered the...
Tipo: Journal article Palavras-chave: Testis; Spermatogenesis; Bisphenol A; Nonylphenol; ADAM17.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100001
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Decreased spermatogenic and androgenic testicular functions in adult rats submitted to immobilization-induced stress from prepuberty BJMBR
Almeida,S.A.; Petenusci,S.O.; Anselmo-Franci,J.A.; Rosa-e-Silva,A.A.M.; Lamano-Carvalho,T.L..
We investigated whether chronic stress applied from prepuberty to full sexual maturity interferes with spermatogenic and androgenic testicular functions. Male Wistar rats (40 days old) were immobilized 6 h a day for 60 days. Following immobilization, plasma concentrations of corticosterone and prolactin increased 135% and 48%, respectively, while plasma luteinizing hormone and testosterone presented a significant decrease of 29% and 37%, respectively. Plasma concentration of follicle-stimulating hormone was not altered in stressed rats. Chronic stress reduced the amount of mature spermatids in the testis by 16% and the spermatozoon concentration in the cauda epididymidis by 32%. A 17% reduction in weight and a 42% decrease in DNA content were observed in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Spermatogenesis; LH; FSH; Testosterone; Prolactin.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100013
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Desenvolvimento gonadal de pirarucu no primeiro ano de vida. Repositório Alice
PEREIRA, A. S.; LIMA, A. F.; KIRSCHNIK, L. N. G.; KIRSCHNIK, P. G..
O objetivo do estudo foi caracterizar o desenvolvimento gonadal do pirarucu no primeiro ano de vida, por meio de análises histológicas. Dez exemplares foram aleatoriamente selecionados para cada classe de tamanho. Os peixes foram fixados e emblocados inteiros e processados rotineiramente para histologia. Para os alevinos das classes I e II, no primórdio gonadal não foi possível observar claramente as células primordiais, apenas uma estrutura ímpar de aglomerado de células localizados na região gonadal. Para os peixes dos Grupos de III e IV, no primórdio gonadal já foi observado predominantemente células somáticas com núcleo ovalado e raras células germinativas primordiais. Em juvenis do Grupo V e VI também foi evidente no testículo os aglomerados de...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Pirarucu; Peixe; Espermatogênese; Fish; Arapaima gigas; Spermatogenesis; Spermatogonia.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115178
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Differential expression and localization of ADAM10 and ADAM17 during rat spermatogenesis suggest a role in germ cell differentiation Biol. Res.
Urriola-Muñoz,Paulina; Lizama,Carlos; Lagos-Cabré,Raúl; Reyes,Juan G; Moreno,Ricardo D.
BACKGROUND: Extracellular metolloproteases have been implied in different process such as cell death, differentiation and migration. Membrane-bound metalloproteases of the ADAM family shed the extracellular domain of many cytokines and receptor controlling auto and para/juxtacrine cell signaling in different tissues. ADAM17 and ADAM10 are two members of this family surface metalloproteases involved in germ cell apoptosis during the first wave of spermatogenesis in the rat, but they have other signaling functions in somatic tissues. RESULTS: In an attempt to further study these two enzymes, we describe the presence and localization in adult male rats. Results showed that both enzymes are detected in germ and Sertoli cells during all the stages of...
Tipo: Journal article Palavras-chave: Testis; Spermatogenesis; Apoptosis; Paracrine; Metalloproteinase.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100031
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Effect of Flutamide in Mouse Spermatogenesis and on the Function of Seminal Vesicle and Prostate International Journal of Morphology
Bustos-Obregón,Eduardo; Esponda,Pedro; Sarabia,Luis.
The spermatogenesis is regulated by the hypotalamus-hypophysis gonadal axis, and the androgens play a fundamental role in their last stages. The administration of the antiandrogen flutamide interferes with the function of androgen dependent organs (Prostate (P) and Seminal Vesicle (VS)). In this study flutamide (10mg/Kg b.w.) was injected to 10 mice and vehicle to the controls (n=6). The mice were sacrificed at 24 (n=5) and 72 (n=5) hours. In testicular sections the tubular diameter (TD) and height of seminiferous epithelium (EH) were measured. P and VS were macerated, and concentrations of fructose (VS) and zinc (P) were determined. No significant difference exists in the DT among the groups. However, the 72 hrs group showed EH higher than the control (p...
Tipo: Journal article Palavras-chave: Flutamide; Mouse; Spermatogenesis; Seminal vesicles; Prostate.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300008
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Effect of GnRH on scrotal surface temperature, testicular volume and spern parameters of bulls with poor semen quality. Repositório Alice
ROMANELLO, N.; BOTTA, D.; MOURA, A. B. B.; PANTOJA, M. H. de A.; BARRETO, A. do N.; SOUZA, M. A. de P.; VOLANTE, C. A.; GARCIA, A. R..
Spermatogenesis is coordinated by the hypothalamic-pituitary-gonadal axis, mainly by GnRH secretion.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Scrotal thermoregulation system; Testicular volume; Seminal parameters; Spermatogenesis.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1101234
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Effect of pinealectomy, adrenalectomy, pinealectomy plus adrenalectomy upon the quantification of spermatogenic cells of adult rats Arq. Bras. Med. Vet. Zootec.
Castro,A.C.S.; Queiroz,G.F.; Nogueira,J.C.; Coimbra,C.C.; Reis,A.M.; Marubayashi,U.; Yamasaki,K..
The objectives of this study were to evaluate the effects of pinealectomy, adrenalectomy and pinealectomy-adrenalectomy upon the quantification of spermatogenic cells of rats. As such, 32 adult Wistar rats with a mean body weight of 331.7± 15.5g were assigned into one of the following treatments: (a) a sham-operated control group, consisting of nine animals; (b) ten pinealectomized animals; (c) seven adrenalectomized animals and (d) six pinealectomized plus adrenalectomized animals. No significant differences were observed between groups for the following parameters: body, testes, prostate and seminal vesicle weights, seminiferous tubular diameter, number of cells per seminiferous tubular cross sections (primary spermatocytes at pachytene, round...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spermatogenesis; Pinealectomy; Adrenalectomy; Rat.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000300008
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Effect of Prolonged Oral Administration of Aqueous Ficus sycomorus Stem-bark Extract on Testicular Size of Growing Albino Rat International Journal of Morphology
Igbokwe,N. A; Igbokwe,I. O; Sandabe,U. K.
Decoction of Ficus sycomorus stem bark is used to treat infertility involving low sperm counts, but the reproductive risk associated with its use is uncertain. The aqueous stem-bark extract was orally administered at 200-600 mg/kg for 30 days to post-pubertal growing albino rats. Within the period, the live body weights, scrotal diameters and testicular size parameters were estimated. The mean live body weight, scrotal diameter of the control and treated rats significantly (p<0.05) increased as the animals grew, but the treatment did not significantly (p>0.05) affect the parameters. There were no significant (p>0.05) variations in mean testicular size parameters in treated and control rats, and no dose-dependent nor time-dependent effects...
Tipo: Journal article Palavras-chave: Ficus sycomorus extract; Testicular size; Testicular histopathology; Spermatogenesis; Male reproductive risk.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000400052
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Effects of catuaba cristal® on the testis of wistar rats BABT
Freitas,Karine Moura de; Costa,Kyvia Lugate; Campos,Pamela Kelly Araújo; Melo,Fabiana Cristina Silveira Alves de; Paula,Tarcízio Antônio Rego de; Matta,Sérgio Luis Pinto da.
The aim of this study was to evaluate the possible beneficial effects of Catuaba Cristal® (CC), an alcoholic drink made from wine and Erythroxylum catuaba Ar. Cam on testis. Wistar rats either received CC solution (n=8) or water (n=9). Results showed significant body weight reduction within the CC group, although, no weight changes were observed for liver, kidney, testis, epididymis, seminal vesicle and prostate. The volumetric proportion and volume of interstitial tissue and lymphatic space were reduced in the treated group. In the CC group, although the nuclear volume of Leydig cells (LC) decreased, the number of LC per testis increased. These results suggested that CC had no beneficial effect on spermatogenesis of Wistar rats.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aphrodisiac drink; Medicinal plants; Morphometry; Spermatogenesis; Testis.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500007
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Effects of Nandrolone Decanoate on Spermatogenesis in Wistar Rats With and Without Physical Training International Journal of Morphology
Maia,Bernardo Porto; Silveira,Jéssica de Oliveira; Borges,Mariana Garcia Lisboa; Pereira,Danilo Ales Ponzi; Pinheiro,Julyara Lima; Freitas,Jofre Jacob da Silva; Kietzer,Katia Simone.
Androgenic anabolic steroids (AAS) are artificial testosterone analogues, used as medicine in chronic diseases, because they increase protein synthesis generating muscle hypertrophy. Its effect has caught the attention of athletes and gym users, thus their consumption has become epidemic, due to easy marketing, the immediate results and the false impression that it doesn't carry health risks. Such risks may globally harm the body. This study aims to investigate the influence on spermatogenesis of using nandrolone decanoate with or without physical training. Twenty-four rats, divided into four groups were used: sedentary group (SG), sedentary on steroids group (SSG), trained group (TG) and trained on steroids group (TSG). The animals were trained on...
Tipo: Journal article Palavras-chave: Spermatogenesis; Anabolic agents; Nandrolone; Exercise; Fertility.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300046
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Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus). Repositório Alice
PINTO, T. M. F.; MOREIRA, R. F.; MATOS, M. N. C.; SOARES, V. V. M.; AGUIAR, M. V. de A.; ARAGÃO, P. de T. T. D. de; ALVES FILHO, J. G.; MORENO, F. B. M. B.; MONTEIRO-MOREIRA, A. C. de O.; COSTA, C. R. R.; LIMA FILHO, J. L. de; ELOY, A. M. X.; CUNHA, R. M. S. da.
Abstract: The Saanen goat breed has been widely explored in breeding programmes; however, there are few reports about the breed?s genetic and molecular composition. Thus, this study aimed to characterize the proteomic profile of spermatozoa from Saanen breeding goats. Five breeding animals with proven fertility were selected, the spermatozoa were collected, and the protein was extracted. Subsequently, the proteins were separated and analysed by two-dimensional electrophoresis and mass spectrometry; the proteins were then identified with the SwissProt database. A total of 31 proteins involved in reproduction were identified, including binding proteins on spermatozoa for fusion with the egg, acrosomal membrane proteins, metabolic enzymes, heat shock...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Sperm; Profiles; Proteomic profile; Brasil; Brazilian Northeast; Caprino; Goats; Saanen; Proteomics; Spermatogenesis; Semiarid zones.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1120171
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Experimental exposure to 3-monochloropropane-1,2-diol from the pre-puberty causes damage in sperm production and motility in adulthood Biological Sciences
Vieira, Kátia Cristina Melo Tavares; Favareto, Ana Paula Alves.
3-Monochloropropane-1,2-diol (3-MCPD) is a food contaminant that can be formed during the thermic processing of various foodstuffs. Studies of reproductive toxicology of 3-MCPD are mainly concentrated in the evaluation of possible insults caused by exposure of adult animals. However, the prepuberty might be a period of different susceptibility to chemicals. The aim of this study was to evaluate the effects on reproductive endpoints of the 3-MCPD-exposure prepubertal male rats. Wistar male rats were assigned to 4 groups: control and exposed to 2.5; 5 or 10 mg kg-1 day-1 of 3-MCPD for 30 days by gavage. Testis and epididymis were used for sperm counts and histology analysis. Sertoli cell number and dynamic of the spermatogenesis were evaluated. Sperm were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.06.00.00-3 monochloropropanol; Puberty; Rats; Spermatogenesis; Reproduction. Morfologia.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31323
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