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Electron paramagnetic resonance study of lipid and protein membrane components of erythrocytes oxidized with hydrogen peroxide BJMBR
Mendanha,S.A.; Anjos,J.L.V.; Silva,A.H.M.; Alonso,A..
Electron paramagnetic resonance (EPR) spectroscopy of spin labels was used to monitor membrane dynamic changes in erythrocytes subjected to oxidative stress with hydrogen peroxide (H2O2). The lipid spin label, 5-doxyl stearic acid, responded to dramatic reductions in membrane fluidity, which was correlated with increases in the protein content of the membrane. Membrane rigidity, associated with the binding of hemoglobin (Hb) to the erythrocyte membrane, was also indicated by a spin-labeled maleimide, 5-MSL, covalently bound to the sulfhydryl groups of membrane proteins. At 2% hematocrit, these alterations in membrane occurred at very low concentrations of H2O2 (50 µM) after only 5 min of incubation at 37°C in azide phosphate buffer, pH 7.4. Lipid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Electron paramagnetic resonance; Spin label; Erythrocyte; Membrane fluidity; Membrane oxidation; Protein oxidation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600001
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Structural properties of lipid reconstructs and lipid composition of normotensive and hypertensive rat vascular smooth muscle cell membranes BJMBR
Oliveira,T.R.; Lamy,M.T.; De Paula,U.M.; Guimarães,L.L.; Toledo,M.S.; Takahashi,H.K.; Straus,A.H.; Lindsey,C.J.; Paiva,T.B..
Multiple cell membrane alterations have been reported to be the cause of various forms of hypertension. The present study focuses on the lipid portion of the membranes, characterizing the microviscosity of membranes reconstituted with lipids extracted from the aorta and mesenteric arteries of spontaneously hypertensive (SHR) and normotensive control rat strains (WKY and NWR). Membrane-incorporated phospholipid spin labels were used to monitor the bilayer structure at different depths. The packing of lipids extracted from both aorta and mesenteric arteries of normotensive and hypertensive rats was similar. Lipid extract analysis showed similar phospholipid composition for all membranes. However, cholesterol content was lower in SHR arteries than in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Smooth muscle cell lipids; Membrane microviscosity; Spontaneously hypertensive rats; Lipid composition; Spin label; Electron spin resonance.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900012
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