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Visualization of avian influenza virus infected cells using self-assembling fragments of green fluorescent protein Electron. J. Biotechnol.
Kanehira,Katsushi; Uchida,Yuko; Saito,Takehiko.
Background: Avian influenza viruses (AIVs) are influenza A viruses which are isolated from domestic and wild birds. AIVs that include highly pathogenic avian influenza viruses (HPAIVs) are a major concern to the poultry industry because they cause outbreaks in poultry with extraordinarily high lethality. In addition, AIVs threaten human health by occasional zoonotic infection of humans from birds. Tools to visualize AIV-infected cells would facilitate the development of diagnostic tests and preventative methods to reduce the spread of AIVs. In this study, a self-assembling split-green fluorescent protein (split-GFP) system, combined with influenza virus reverse genetics was used to construct a visualization method for influenza virus-infected cells....
Tipo: Journal article Palavras-chave: Avian influenza virus; Reverse genetics; Split-GFP.
Ano: 2016 URL:
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