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| Monteiro,G.A.; Guasti,P.N.; Hartwig,F.P.; Dellaqua Jr.,J.A.; Alvarenga,M.A.; Papa,F.O.. |
| After a serious injury or sudden death, epididymis cauda sperm recovery and cryopreservation may present as the last opportunity to obtain genetic material from a valuable stallion. This study evaluated the viability of cooled equine sperm collected by three different methods: sperm of ejaculated (G1), sperm recovered from the epididymal cauda immediately after orchiectomy (G2) and sperm recovered from the epididymal cauda after storage for 24 hours at 5°C (G3). To obtain G1 sperm, two ejaculates were collected. After 1 week, all stallions underwent a bilateral orchiectomy, and one of the removed epididymides was flushed to obtain G2 sperm. The contralateral epididymis was stored at 5°C for 24 hours before being flushed to obtain G3 sperm. The sperm... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Horse epididymal spermatozoa; Cryopreservation; Stallion; Sperm viability; Seminal plasma. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000300010 |
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| Celeghini,Eneiva Carla Carvalho; Andrade,André Furugen Cesar de; Raphael,Cláudia Fernandes; Nascimento,Juliana; Ticianelli,Janahi Souza; Arruda,Rubens Paes de. |
| To validate a practical technique of simultaneous evaluation of the plasma, acrosomal and mitochondrial membranes in equine spermatozoa three fluorescent probes (PI, FITC-PSA and MITO) were associated. Four ejaculates from three stallions (n=12) were diluted in TALP medium and split into 2 aliquots, 1 aliquot was flash frozen in liquid nitrogen to induce damage in cellular membranes. Three treatments were prepared with the following fixed ratios of fresh semen: flash frozen semen: 100:0 (T100), 50:50 (T50), and 0:100 (T0). A 150-µL aliquot of diluted semen of each treatment was added of 2 µL of PI, 2 µL of MITO and 80 µL of FITC-PSA; incubated at 38.5ºC/8 min, and sperm cells were evaluated by epifluorescent microscopy. Based in regression analysis, this... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stallion; Acrosome; Plasma membrane; Mitochondria; Fluorescent probes. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600004 |
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| Ferreira,C.; Costa,E.A.; França,S.A.; Melo,U.P. de; Drumond,B.P.; Bomfim,M.R.Q.; Coelho,F.M.; Resende,M.; Palhares,M.S.; Santos,R.L.. |
| Em agosto de 2008, um garanhão da raça Mangalarga Marchador, de quatro anos de idade, com histórico clínico de apatia, inapetência e edema de prepúcio e escroto, apresentou, ao exame clínico, exsudato purulento fluindo pelo óstio prepucial, prepúcio e mucosa peniana com inúmeras lesões circulares de bordos elevados e hiperêmicos, centro ulcerado recoberto por material amarelado de aspecto fibrinoso, com distribuição multifocal. Histologicamente, a mucosa peniana apresentou áreas de ulceração associadas a infiltrado inflamatório misto, com necrose multifocal e moderado acúmulo de fibrina, que se estendiam para o tecido conjuntivo adjacente. O diagnóstico morfológico foi de balanopostite ulcerativa fibrino-necrótica multifocal intensa, similar ao encontrado... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Stallion; Equine herpesvirus 3; Erosive-ulcerative skin lesion; Prepucial region. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600035 |
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| Gheller,S.M.M.; Corcini,C.D.; Santos,F.C.C.; Tavares,G.C.; Costa,V.G.G.; Curcio,B.R.; Nogueira,C.E.W.; Varela Junior,A.S.. |
| ABSTRACT This study was designed to evaluate the possible benefits of adding xanthan gum to a standard extender for equine through in vitro analyzes of sperm quality. Semen was collected four times from five different stallions (n= 20 samples) and subjected to cooled storage under different conditions: control (only standard extender) and three different concentrations of xanthan gum (0.01%, 0.12%, and 0.25%) supplemented to the extenders. Sperm parameters, such as motility, mitochondrial functionality, and membrane, acrosome, and DNA integrity were measured after 0h, 24h, 48h, and 72h of sperm storage at 5ºC. Our observations indicated that sperm motility declined with longer cooling period with the 0.25% xanthan gum supplementation group compared with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cooled; Stallion; Semen; Spermatozoa; Storage. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100028 |
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| Pugliesi,Guilherme; Carvalho,Giovanni Ribeiro de; Rates,Daniel Macêdo; Ker,Pedro Gama; Matta,Manuela Pereira da; Oliveira,Renan Reis de; Silva Filho,José Monteiro da. |
| Two semen extenders were compared for their ability to maintain viability of horse semen during 24 hours of cold preservation, and for the pregnancy rate after artificial insemination. In the experiment 1, five ejaculates from three stallions were split-diluted in either a skimmed milk-based extender (Kenney extender) or a glycine egg yolk-based extender (Foote extender) and cooled at 6-8 ºC for 24 hours. Semen samples stored in Kenney extender for 24 hours had higher motility and spermatic vigor compared with those stored in Foote extender. However, samples stored in Foote extender had higher number of reactive sperm by hypoosmotic test and greater viability by epifluorescence test compared with those in Kenney extender. In the experiment 2, 17 and 23... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Artificial insemination; Cooling; Semen extender; Stallion. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001200005 |
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