SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Silva,A.B.; Silva,T.; Franco,E.S.; Rabelo,S.A.; Lima,E.R.; Mota,R.A.; Câmara,C.A.G. da; Pontes-Filho,N.T.; Lima-Filho,J.V..

The antibacterial potential of leaf's essential oil (EO) from Brazilian pepper tree (Schinus terebinthifolius Raddi) against staphylococcal isolates from dogs with otitis externa was evaluated. The minimum inhibitory concentration of EO ranged from 78.1 to 1,250 fg/mL. The oil was analyzed by GC and GC/MS and cytotoxicity tests were carried out with laboratory animals.

Antibacterial activity of extracts of Acacia aroma against methicillin-resistant and methicillin-sensitive Staphylococcus

Palavras-chave: Essential oil; Brazilian pepper tree; Schinus terebinthifolius; Otitis externa; Staphylococcus.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100023

Mattana,C.M.; Satorres,S.E.; Sosa,A.; Fusco,M.; Alcaráz,L.E..

Antibacterial activity of organic and aqueous extracts of Acacia aroma was evaluated against methicillin-resistant Staphylococcus aureus (MRSA), methicillin sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus epidermidis. Inhibition of bacterial growth was determined using agar diffusion and bioautographic methods. Among all assayed organic extracts only ethanolic and ethyl acetate extracts presented highest activities against all tested Staphylococcus strains with minimal inhibitory concentration (MIC) values ranging from 2.5 to 10 mg/ml and from 2.5 to 5 mg/ml respectively. The aqueous extracts show little antibacterial activity against Staphylococcus strains. The bioautography assay demonstrated well-defined growth inhibition...

Anti-staphylococcal effectiveness of nisaplin in refrigerated pizza doughs

Palavras-chave: Acacia aroma; Antibacterial activity; Staphylococcus; Tusca.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300007

BABT

Freitas,Wilma Carla de; Souza,Evandro Leite de; Sousa,Cristina Paiva de; Travassos,Antônio Eustáquio Resende.

This study evaluated the effectiveness of nisaplin, commercial product having nisin as active component, in decreasing the staphylococcal population in refrigerated pizza doughs. The refrigerated pizza dough pieces randomly chosen were dipped in the solutions with nisaplin concentrations of 1.0 x 10-3 g and 1.0 x 10-2 g nisaplin/mL named for the treatment A and B and kept under refrigeration (7 °C ± 1 °C). On times 0, 15 and 30 days post treatment the Staphylococcus spp. count was carried out. The results showed that both nisaplin treatments were able to reduce the Staphylococcus spp. count (CFU/g) in the refrigerated pizza doughs. However, only treatment B showed statistically significant reducer effect (p < 0.05) on the count providing a decrease of...

Atividade in vitro do extrato de própolis contra agentes bacterianos da mastite bovina

Palavras-chave: Nisaplin; Nisin; Dough; Staphylococcus.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300021

PAB

Loguercio,Andrea Pinto; Groff,Ana Cláudia Mello; Pedrozzo,Andréia Folleto; Witt,Niura Mazzini; Silva,Mariana Sá e; Vargas,Agueda Castagna de.

Este trabalho foi desenvolvido com o objetivo de avaliar a atividade in vitro do extrato alcoólico de própolis, contra agentes da mastite bovina, comparando-o aos principais antimicrobianos utilizados no tratamento convencional. Foram utilizados 36 isolados coagulase-positivos de Staphylococcus sp. e 27 isolados de Streptococcus sp.; 94,4% dos Staphylococcus sp. e 85,2% dos Streptococcus sp. foram susceptíveis ao extrato de própolis.

Tipo: Info:eu-repo/semantics/report

Palavras-chave: Staphylococcus; Streptococcus; Susceptibilidade; Antimicrobianos.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000200021

Avaliação da ocorrência de patógenos causadores de mastite subclínica, parâmetros físico-químicos e CCS de amostras de leite caprino.

PINTO, D. dos S.; SOUSA, F. R. de; NOBRE, C. O. R.; MARTINS, P. Y. F.; SOUZA, V. de.

Resumo: Na caprinocultura leiteira a mastite é considerada uma das mais importantes enfermidades, pois afeta a qualidade do leite, diminui a produtividade e eleva os custos de produção. O objetivo desse estudo foi analisar a ocorrência de patógenos causadores de mastite subclínica e avaliar os parâmetros físico-químicos bem como a Contagem de Células Somáticas (CCS) de amostras de leite caprino. Foram coletadas 142 amostras de leite durante o período de junho e julho de 2016, de 20 cabras em lactação, das raças Saanen e Anglo-nubiana. Os resultados médios obtidos para a composição do leite foram 2,4% de gordura; 3,0% de proteína; 4,0% de lactose; 10,2% de sólidos totais; 7,8% de sólidos não gordurosos e 193.000 CS/mL. Das 142 amostras avaliadas...

Bacteria associated with Amblyomma cajennense tick eggs

Palavras-chave: Mamite; Caprino; Leite de cabra; Estafilococo; Patogenicidade; Goat milk; Somatic cell count; Mastitis; Pathogenicity; Streptococcus; Staphylococcus; Brazil.

Ano: 2017

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1075615

Genet. Mol. Biol.

Machado-Ferreira,Erik; Vizzoni,Vinicius Figueiredo; Piesman,Joseph; Gazeta,Gilberto Salles; Soares,Carlos Augusto Gomes.

Abstract Ticks represent a large group of pathogen vectors that blood feed on a diversity of hosts. In the Americas, the Ixodidae ticks Amblyomma cajennense are responsible for severe impact on livestock and public health. In the present work, we present the isolation and molecular identification of a group of culturable bacteria associated with A. cajennense eggs from females sampled in distinct geographical sites in southeastern Brazil. Additional comparative analysis of the culturable bacteria from Anocentor nitens, Rhipicephalus sanguineus and Ixodes scapularis tick eggs were also performed. 16S rRNA gene sequence analyses identified 17 different bacterial types identified as Serratia marcescens, Stenotrophomonas maltophilia, Pseudomonas fluorescens,...

Características microbiológicas da água obtida em propriedades leiteiras no município de Sacramento, MG.

Palavras-chave: Amblyomma cajennense; Tick eggs bacteria; Staphylococcus; Ixodidae.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400477

PINTO, F. de R.; SOUZA, V. de; NADER FILHO, A.; AMARAL, L. A. do.

Os objetivos do trabalho foram avaliar a qualidade microbiológica da água de usos múltiplos e utilizada na produção leiteira em doze propriedades na região da Gameleira, no Município de Sacramento, MG. [Water microbiological characteristics in dairy properties in Sacramento, MG].

Characterization of Staphylococcus spp. strains in milk from buffaloes with mastitis in Brazil: the need to identify to species level to avoid misidentification

Palavras-chave: Produção do leite; Contamination.; Escherichia coli; Qualidade da água; Contaminação; Ordenha; Análise microbiológica; Bactéria; Saneamento rural; Contaminação bacteriana; Water quality; Milk; Milking; Coliform bacteria; Staphylococcus; Brazil..

Ano: 2013

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/958604

Arq. Bras. Med. Vet. Zootec.

Coimbra-e-Souza,V.; Brito,M.A.V.P.; Chamon,R.C.; Laport,M.S.; Giambiagi-deMarval,M..

ABSTRACT Mastitis is an inflammation of the mammary gland that affects dairy cattle worldwide causing economic losses. Coagulase-negative staphylococci (CNS) are the predominant cause of this type of infection. We have recently showed that coagulase-positive staphylococci could be misidentified. So, the aim of this study was to characterize the Staphylococcus spp. strains initially classified as coagulase-negative Staphylococci, isolated from buffalo with subclinical mastitis. Milk of buffaloes with mastitis in herds was collected and 9 strains were identified as CNS by phenotypic tests. Molecular methodologies latter identified the strains as coagulase-negative Staphylococcus chromogenes (5), coagulase-positive Staphylococcus hyicus (2) and...

Complete genome sequences of 11 Staphylococcus sp. Strains Isolated from buffalo milk and milkers? hands.

Palavras-chave: Buffalo; Mastitis; Staphylococcus; Milk; Coagulase test.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000601669

Contagem de Staphylococcus sp. e detecção de enterotoxinas estafilocócicas e toxina da síndrome do choque tóxico em amostras de leite cru refrigerado

PIZAURO, L. J. L.; ALMEIDA, C. C. de; GOHARI, I. M.; MACINNES, J. I.; ZAFALON, L. F.; KROPINSKI, A. M.; VARANI, A. M..

Here, we present data on the complete genome sequences of 11 Staphylococcus sp. isolates (three S. chromogenes isolates and one isolate each of S. saprophyticus, S. xylosus, S. hominis, S. agnetis, S. caprae, S. aureus, and S. warneri), obtained as part of a mastitis study of buffalo milk (from healthy animals and from those with subclinical mastitis) and milkers? hands.

Tipo: Artigo de periódico

Palavras-chave: Staphylococcus; Buffalo milk; Mastitis; Ruminants.

Ano: 2019

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1116621

Arq. Bras. Med. Vet. Zootec.

Lamaita,H.C.; Cerqueira,M.M.O.P.; Carmo,L.S.; Santos,D.A.; Penna,C.F.A.M.; Souza,M.R..

Analisaram-se 80 amostras de leite cru refrigerado a 4°C e estocado por 48 horas em tanques refrigeradores de propriedades rurais do estado de Minas Gerais quanto à contagem e identificação de Staphylococcus sp. e detecção de enterotoxinas estafilocócicas (SE) e da toxina da síndrome do choque tóxico (TSST-1). Staphylococcus sp. foi detectado em 100% das amostras de leite de tanque refrigerador em contagens que variaram de 1,0 × 10(5) a 2,5 × 10(7) UFC/ml (média = 5,60 log UFC/ml; s = 0,53 e CV = 9,5%). Isolaram-se e identificaram-se 436 estirpes como: S. aureus, S hyicus, S. epidermidis, S. intermedius, S. cohnii, S. sciuri, S. schleirferi e S. delphini. As estirpes de mesmo perfil bioquímico, oriundas da mesma amostra, foram agrupadas (pools) e induzidas...

Detecção de Staphylococcus sp em leite de cabra com e sem a utilização de boas práticas de ordenha.

Palavras-chave: Leite cru; Staphylococcus; Enterotoxina; TSST-1.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000500017

Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates

CHAPAVAL, L.; VIANA, G. A.; SOUSA, A. P. G. de; AGUIAR, V. N. P.; MIRANDA, K. P. de; MORORÓ, A. M..

Os alimentos de origem animal são reconhecidos pelo seu alto valor nutritivo, e também pelo alto potencial para provocar casos de intoxicação alimentar devido a serem veiculadores de microrganismos que podem ser patogênicos. A carga microbiana inicial do leite é ponto chave para um produto final de qualidade e, dentre as etapas de produção de leite de cabra, a ordenha pode ser considerada uma das tarefas mais importantes dentro de uma propriedade leiteira. Este trabalho demonstrou a importância do uso de Boas Práticas Agropecuárias como uma poderosa ferramenta para controle de perigos microbiológicos na produção primária.

Tipo: Parte de livro

Palavras-chave: Boas práticas; Caprino; Leite de cabra; Segurança alimentar; Ordenha; Estafilococo; Goat milk; Staphylococcus; Milking.

Ano: 2009

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/576408

BJID

Coutinho,Vivian de Lima Spode; Paiva,Rodrigo Minuto; Reiter,Keli Cristine; de-Paris,Fernanda; Barth,Afonso Luis; Machado,Alice Beatriz Mombach Pinheiro.

INTRODUCTION: Resistance to macrolides, lincosamides and streptogramins B (MLS B antibiotics) in staphylococci may be due to modification in ribosomal target methylase encoded by erm genes. The expression of MLS B resistance lead to three phenotypes, namely constitutive resistance (cMLS B), inducible resistance (iMLS B), and resistance only to macrolides and streptogramins B (MS B). The iMLS B resistance is the most difficult to detect in the clinical laboratory. OBJECTIVE: This study investigated the expression of MLS B resistance and the prevalence of the erm genes among 152 clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus (CNS) from Hospital de Clínicas de Porto Alegre. METHODS: Primary MLS B resistance was detected by...

Effect of chitosan coating on contamination of fresh bovine meat sold in the open market

Palavras-chave: Staphylococcus; Resistance; Erm genes; Macrolides.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600004

Rev. Ciênc. Agron.

Silva,Alessandra Santana; Sampaio,Adriana Pereira; Santos,Marly Silveira; Souza,Bartolomeu Warlene Silva de; Evangelista-Barreto,Norma Suely.

ABSTRACT This study aimed to evaluate the sanitary status of fresh bovine meat sold at the open market in Jiquiriça municipality, Brazil and validate the use of chitosan as an antimicrobial coating to control the bioburden. In total, we evaluated 30 samples of fresh beef for the presence of coagulase-positive staphylococci and Salmonella spp. Furthermore, stores selling fresh meat products were evaluated for compliance with sanitary regulations set by the Ministry of Health in Brazil. To evaluate the antimicrobial activity of chitosan two sets of meat samples were tested, the test group consisted of contaminated meat coated with 2% chitosan solution whereas the control group consisted of untreated contaminated meat. It was found that fresh bovine meat at...

Enterotoxigenicidade de Staphylococcus spp. isolados de leite in natura

Palavras-chave: Salmonella; Staphylococcus; Internal meat temperature; Antimicrobial activity.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902019000100038

Ciênc. Tecnol. Aliment.

Stamford,Tânia Lúcia Montenegro; Silva,Celiane Gomes Maia da; Mota,Rinaldo Aparecido; Cunha Neto,Adelino da.

Os alimentos são passíveis de contaminação por diferentes agentes etiológicos, podendo levar a doenças manifestadas por ação de microorganismos patogênicos ou suas toxinas. Pesquisou-se a ocorrência de cepas de Staphylococcus, assim como a sua capacidade para produção de enterotoxinas em leite produzido e/ou comercializado no Estado de Pernambuco, Brasil. Foram isoladas e selecionadas 109 cepas de Staphylococcus coagulase positiva e negativa de leite in natura. A identificação das cepas isoladas foi realizada por meio de testes morfológicos e bioquímicos, como: testes de catalase, coagulase, hemólise, DNAse, termonuclease, produção de acetoína (VP) e metabolismo de carboidratos (glicose, maltose e manitol). Das 77 cepas coagulase positivas foram...

Enteroxigenic Staphylococcus spp. and other microbial contaminants during production of Canastra cheese, Brazil

Palavras-chave: Enterotoxinas; Staphylococcus; Alimentos; Leite.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612006000100007

Borelli,Beatriz M.; Ferreira,Elaine G.; Lacerda,Inayara C.A.; Santos,Deise A.; Carmo,Luiz S.; Dias,Ricardo S.; Silva,Maria Crisolita C.; Rosa,Carlos A..

Canastra cheese is produced from raw cow's milk, and it is made at the farmhouse level using artisanal procedures and natural starters. The aim of this work was to determine the main hygienic-sanitary indicators and enterotoxigenic staphylococcal strains present during the manufacturing of traditional cheese of Serra da Canastra region, Minas Gerais state, Brazil. Samples from 10 farms were studied, and they included: water employed in the process, raw milk, natural starters, cheese curd before salting and cheese after five days of ripening. All water samples exhibited faecal coliform contamination above the maximum acceptable value recommended by Brazilian standards. Pseudomonas aeruginosa and sulfite-reducing clostridia were also isolated from the water...

Genomic comparisons and phylogenetic analysis of mastitis‑related staphylococci with a focus on adhesion, bioflm, and related regulatory genes.

Palavras-chave: Artisanal cheese; Coliforms; Staphylococcus; Enterotoxin.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400026

Genotypic and phenotypic characterization of antimicrobial resistance in Staphylococcus Spp. isolated from cows after alternative treatment of bovine mastitis.

PIZAURO, L. J. L.; ALMEIDA, C. C. DE; SILVA, S. R.; MACINNES, J. I.; KROPINSKI, A. M.; ZAFALON, L. F.; AVILA, F. A. DE; VARANI, A. DE M..

Mastitis is a common and costly disease on dairy farms, commonly caused by Staphylococcus spp. though the various species are associated with different clinical outcomes. In the current study, we performed genomic analyses to determine the prevalence of adhesion, biofilm, and related regulatory genes in 478 staphylococcal species isolated from clinical and subclinical mastitis cases deposited in public databases. The most prevalent adhesin genes (ebpS, atl, pls, sasH and sasF) were found in both clinical and subclinical isolates. However, the ebpS gene was absent in subclinical isolates of Staphylococcus arlettae, S. succinus, S. sciuri, S. equorun, S. galinarum, and S. saprophyticus. In contrast, the coa, eap, emp, efb, and vWbp genes were present more...

Tipo: Artigo de periódico

Palavras-chave: Biofim operon; IcaABCD; IcaR; Regulatory genes; Mastitis; Staphylococcus; Staphylococcus arlettae; Staphylococcus succinus; Staphylococcus sciuri; Staphylococcus equorum; Staphylococcus gallinarum; Staphylococcus saprophyticus; Adhesins; Vaccine development.

Ano: 2021

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137199

FERREIRA, E. M.; ROMERO, L. C.; SILVA, L. P.; PEREIRA, L. E. C.; MALAGO JUNIOR, W.; CHAPAVAL, L.; CUNHA, M. de L. R. de S. da; ZAFALON, L. F..

Staphylococcus spp. is among the major microorganisms involved in the etiology of bovine mastitis, with the ability to quickly acquire antimicrobial resistance genes.

Habituation of enterotoxigenic Staphylococcus aureus to Origanum vulgare L. essential oil does not induce direct-tolerance and cross-tolerance to salts and organic acids

Palavras-chave: Homeopathy; SCCmeC; S epidermidis; MecA; BlaZ; Staphylococcus.

Ano: 2019

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1116609

Tavares,Adassa Gama; Monte,Daniel Farias Marinho do; Albuquerque,Allan dos Reis; Sampaio,Fábio Correia; Magnani,Marciane; Siqueira Júnior,José Pinto de; Souza,Evandro Leite de.

Enterotoxigenic Staphylococcus aureus strains that were isolated from foods were investigated for their ability to develop direct-tolerance and cross-tolerance to sodium chloride (NaCl), potassium chloride (KCl), lactic acid (LA) and acetic acid (AA) after habituation in sublethal amounts (1/2 of the minimum inhibitory concentration - 1/2 MIC and 1/4 of the minimum inhibitory concentration - 1/4 MIC) of Origanum vulgare L. essential oil (OVEO). The habituation of S. aureus to 1/2 MIC and 1/4 MIC of OVEO did not induce direct-tolerance or cross-tolerance in the tested strains, as assessed by modulation of MIC values. Otherwise, exposing the strains to OVEO at sublethal concentrations maintained or increased the sensitivity of the cells to the tested...

Identificação e caracterização bioquímica de estirpes de Staphylococcus spp. isoladas de amostras de leite de cabra.

Palavras-chave: Staphylococcus; Adaptation; Essential oil; Oregano.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300835

COSTA, V. M. D.; FERNANDES, D. R.; SILVA, L. M. F. da; MELO, P. de C.; SOUZA, V. de.

Resumo: O leite de cabra apresenta-se como uma importante alternativa para diminuir a desnutrição, devido suas características nutricionais, mas para ser consumido necessita de padrões de qualidade exigidos pela legislação vigente. Uma patologia que interfere diretamente na produção do leite é a mastite, principalmente subclínica, pois além de diminuir a produção, ainda causa danos a saúde da glândula mamária. Objetivou-se nesse trabalho identificar e caracterizar bioquimicamente estirpes de Staphylococcus spp. isoladas de amostras de leite de cabra. Foram colhidas 240 amostras de leite de 30 animais que não apresentavam sinais evidentes de mastite clínica e levadas ao Laboratório de Microbiologia da Embrapa Caprinos e Ovinos para isolamento e...

Identification and antimicrobial susceptibility of Staphylococcus from home-treated peritoneal dialysis patients

Palavras-chave: Coagulase; Positive-coagulase; Bacteriological analysis.; Caprino; Leite de cabra; Mamite; Análise bacteriológica; Estafilococo; Infecção; Glandula mamária; Goat milk; Staphylococcus; Mastitis; Mammary gland diseases; Brazil..

Ano: 2015

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1022767

J. Venom. Anim. Toxins incl. Trop. Dis.

Batalha,JEN; Cunha,MLRS; Montelli,AC; Barreti,P; Caramori,JCT.

Staphylococcus aureus is the main agent of infections during peritoneal dialysis (PD). The presence of S. aureus in the nasal cavity has been extensively studied and suggested as a risk factor of dialysis-related infections, whereas coagulase-negative Staphylococcus (CNS) species are frequently considered part of the normal human microbiota. The aim of this study was to identify Staphylococcus in the nasal cavity, pericatheter skin and peritoneal effluent from PD patients, as well as to evaluate the antimicrobial activity evolution in vitro. Thirty-two chronic PD patients were observed during 12 months and had nasal and pericatheter skin samples collected for culture. When peritonitis was detected, samples were also collected from the peritoneal effluent...