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Antibacterial activity, chemical composition, and cytotoxicity of leaf's essential oil from Brazilian pepper tree (Schinus terebinthifolius, Raddi) BJM
Silva,A.B.; Silva,T.; Franco,E.S.; Rabelo,S.A.; Lima,E.R.; Mota,R.A.; Câmara,C.A.G. da; Pontes-Filho,N.T.; Lima-Filho,J.V..
The antibacterial potential of leaf's essential oil (EO) from Brazilian pepper tree (Schinus terebinthifolius Raddi) against staphylococcal isolates from dogs with otitis externa was evaluated. The minimum inhibitory concentration of EO ranged from 78.1 to 1,250 fg/mL. The oil was analyzed by GC and GC/MS and cytotoxicity tests were carried out with laboratory animals.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Essential oil; Brazilian pepper tree; Schinus terebinthifolius; Otitis externa; Staphylococcus.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100023
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Antibacterial activity of extracts of Acacia aroma against methicillin-resistant and methicillin-sensitive Staphylococcus BJM
Mattana,C.M.; Satorres,S.E.; Sosa,A.; Fusco,M.; Alcaráz,L.E..
Antibacterial activity of organic and aqueous extracts of Acacia aroma was evaluated against methicillin-resistant Staphylococcus aureus (MRSA), methicillin sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus epidermidis. Inhibition of bacterial growth was determined using agar diffusion and bioautographic methods. Among all assayed organic extracts only ethanolic and ethyl acetate extracts presented highest activities against all tested Staphylococcus strains with minimal inhibitory concentration (MIC) values ranging from 2.5 to 10 mg/ml and from 2.5 to 5 mg/ml respectively. The aqueous extracts show little antibacterial activity against Staphylococcus strains. The bioautography assay demonstrated well-defined growth inhibition...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acacia aroma; Antibacterial activity; Staphylococcus; Tusca.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300007
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Anti-staphylococcal effectiveness of nisaplin in refrigerated pizza doughs BABT
Freitas,Wilma Carla de; Souza,Evandro Leite de; Sousa,Cristina Paiva de; Travassos,Antônio Eustáquio Resende.
This study evaluated the effectiveness of nisaplin, commercial product having nisin as active component, in decreasing the staphylococcal population in refrigerated pizza doughs. The refrigerated pizza dough pieces randomly chosen were dipped in the solutions with nisaplin concentrations of 1.0 x 10-3 g and 1.0 x 10-2 g nisaplin/mL named for the treatment A and B and kept under refrigeration (7 °C ± 1 °C). On times 0, 15 and 30 days post treatment the Staphylococcus spp. count was carried out. The results showed that both nisaplin treatments were able to reduce the Staphylococcus spp. count (CFU/g) in the refrigerated pizza doughs. However, only treatment B showed statistically significant reducer effect (p < 0.05) on the count providing a decrease of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nisaplin; Nisin; Dough; Staphylococcus.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300021
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Atividade in vitro do extrato de própolis contra agentes bacterianos da mastite bovina PAB
Loguercio,Andrea Pinto; Groff,Ana Cláudia Mello; Pedrozzo,Andréia Folleto; Witt,Niura Mazzini; Silva,Mariana Sá e; Vargas,Agueda Castagna de.
Este trabalho foi desenvolvido com o objetivo de avaliar a atividade in vitro do extrato alcoólico de própolis, contra agentes da mastite bovina, comparando-o aos principais antimicrobianos utilizados no tratamento convencional. Foram utilizados 36 isolados coagulase-positivos de Staphylococcus sp. e 27 isolados de Streptococcus sp.; 94,4% dos Staphylococcus sp. e 85,2% dos Streptococcus sp. foram susceptíveis ao extrato de própolis.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Staphylococcus; Streptococcus; Susceptibilidade; Antimicrobianos.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000200021
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Bacteria associated with Amblyomma cajennense tick eggs Genet. Mol. Biol.
Machado-Ferreira,Erik; Vizzoni,Vinicius Figueiredo; Piesman,Joseph; Gazeta,Gilberto Salles; Soares,Carlos Augusto Gomes.
Abstract Ticks represent a large group of pathogen vectors that blood feed on a diversity of hosts. In the Americas, the Ixodidae ticks Amblyomma cajennense are responsible for severe impact on livestock and public health. In the present work, we present the isolation and molecular identification of a group of culturable bacteria associated with A. cajennense eggs from females sampled in distinct geographical sites in southeastern Brazil. Additional comparative analysis of the culturable bacteria from Anocentor nitens, Rhipicephalus sanguineus and Ixodes scapularis tick eggs were also performed. 16S rRNA gene sequence analyses identified 17 different bacterial types identified as Serratia marcescens, Stenotrophomonas maltophilia, Pseudomonas fluorescens,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amblyomma cajennense; Tick eggs bacteria; Staphylococcus; Ixodidae.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400477
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Characterization of Staphylococcus spp. strains in milk from buffaloes with mastitis in Brazil: the need to identify to species level to avoid misidentification Arq. Bras. Med. Vet. Zootec.
Coimbra-e-Souza,V.; Brito,M.A.V.P.; Chamon,R.C.; Laport,M.S.; Giambiagi-deMarval,M..
ABSTRACT Mastitis is an inflammation of the mammary gland that affects dairy cattle worldwide causing economic losses. Coagulase-negative staphylococci (CNS) are the predominant cause of this type of infection. We have recently showed that coagulase-positive staphylococci could be misidentified. So, the aim of this study was to characterize the Staphylococcus spp. strains initially classified as coagulase-negative Staphylococci, isolated from buffalo with subclinical mastitis. Milk of buffaloes with mastitis in herds was collected and 9 strains were identified as CNS by phenotypic tests. Molecular methodologies latter identified the strains as coagulase-negative Staphylococcus chromogenes (5), coagulase-positive Staphylococcus hyicus (2) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Buffalo; Mastitis; Staphylococcus; Milk; Coagulase test.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000601669
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Contagem de células somáticas e california mastitis test no diagnóstico da mastite caprina subclínica. Infoteca-e
SILVA, E. R. da; SAUKAS, T. N.; ALVES, F. S. F.; PINHEIRO, R. R..
A mastite e uma doenca altamente prevalente em rebalnhos leiteiros de todo o mundo. Os agentes causais das mastites em caprinos sao semelhantes aqueles da doenca em bovinos, sendo os Staphylococcus coagulase positiva os mais frequentes patogenos associados a forma clinica e os Staphylococcus coagulase negativa a forma subclinica da mastite caprina. O diagnostico da mastite clinica e facil, baseado primariamente nos sinais clinicos; entretanto, o diagnostico da forma subclinica e um problema, tendo-se como metodos auxiliares os testes fisico-quimicos do leite e os baseados no aumento do conteudo celular da glandula mamaria. Por serem rapidos e de facil execucao, os testes baseados no aumento celular do leite sao muito utilizados, sendo o California Mastitis...
Tipo: Folhetos Palavras-chave: Caprino; Mamite; Diagnóstico; Goats; Mastitis; California mastitis test; Staphylococcus; Disease diagnosis.
Ano: 1996 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/514270
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Contagem de Staphylococcus sp. e detecção de enterotoxinas estafilocócicas e toxina da síndrome do choque tóxico em amostras de leite cru refrigerado Arq. Bras. Med. Vet. Zootec.
Lamaita,H.C.; Cerqueira,M.M.O.P.; Carmo,L.S.; Santos,D.A.; Penna,C.F.A.M.; Souza,M.R..
Analisaram-se 80 amostras de leite cru refrigerado a 4°C e estocado por 48 horas em tanques refrigeradores de propriedades rurais do estado de Minas Gerais quanto à contagem e identificação de Staphylococcus sp. e detecção de enterotoxinas estafilocócicas (SE) e da toxina da síndrome do choque tóxico (TSST-1). Staphylococcus sp. foi detectado em 100% das amostras de leite de tanque refrigerador em contagens que variaram de 1,0 × 10(5) a 2,5 × 10(7) UFC/ml (média = 5,60 log UFC/ml; s = 0,53 e CV = 9,5%). Isolaram-se e identificaram-se 436 estirpes como: S. aureus, S hyicus, S. epidermidis, S. intermedius, S. cohnii, S. sciuri, S. schleirferi e S. delphini. As estirpes de mesmo perfil bioquímico, oriundas da mesma amostra, foram agrupadas (pools) e induzidas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leite cru; Staphylococcus; Enterotoxina; TSST-1.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000500017
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Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates BJID
Coutinho,Vivian de Lima Spode; Paiva,Rodrigo Minuto; Reiter,Keli Cristine; de-Paris,Fernanda; Barth,Afonso Luis; Machado,Alice Beatriz Mombach Pinheiro.
INTRODUCTION: Resistance to macrolides, lincosamides and streptogramins B (MLS B antibiotics) in staphylococci may be due to modification in ribosomal target methylase encoded by erm genes. The expression of MLS B resistance lead to three phenotypes, namely constitutive resistance (cMLS B), inducible resistance (iMLS B), and resistance only to macrolides and streptogramins B (MS B). The iMLS B resistance is the most difficult to detect in the clinical laboratory. OBJECTIVE: This study investigated the expression of MLS B resistance and the prevalence of the erm genes among 152 clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus (CNS) from Hospital de Clínicas de Porto Alegre. METHODS: Primary MLS B resistance was detected by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus; Resistance; Erm genes; Macrolides.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600004
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Effect of chitosan coating on contamination of fresh bovine meat sold in the open market Rev. Ciênc. Agron.
Silva,Alessandra Santana; Sampaio,Adriana Pereira; Santos,Marly Silveira; Souza,Bartolomeu Warlene Silva de; Evangelista-Barreto,Norma Suely.
ABSTRACT This study aimed to evaluate the sanitary status of fresh bovine meat sold at the open market in Jiquiriça municipality, Brazil and validate the use of chitosan as an antimicrobial coating to control the bioburden. In total, we evaluated 30 samples of fresh beef for the presence of coagulase-positive staphylococci and Salmonella spp. Furthermore, stores selling fresh meat products were evaluated for compliance with sanitary regulations set by the Ministry of Health in Brazil. To evaluate the antimicrobial activity of chitosan two sets of meat samples were tested, the test group consisted of contaminated meat coated with 2% chitosan solution whereas the control group consisted of untreated contaminated meat. It was found that fresh bovine meat at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Staphylococcus; Internal meat temperature; Antimicrobial activity.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902019000100038
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Enterotoxigenicidade de Staphylococcus spp. isolados de leite in natura Ciênc. Tecnol. Aliment.
Stamford,Tânia Lúcia Montenegro; Silva,Celiane Gomes Maia da; Mota,Rinaldo Aparecido; Cunha Neto,Adelino da.
Os alimentos são passíveis de contaminação por diferentes agentes etiológicos, podendo levar a doenças manifestadas por ação de microorganismos patogênicos ou suas toxinas. Pesquisou-se a ocorrência de cepas de Staphylococcus, assim como a sua capacidade para produção de enterotoxinas em leite produzido e/ou comercializado no Estado de Pernambuco, Brasil. Foram isoladas e selecionadas 109 cepas de Staphylococcus coagulase positiva e negativa de leite in natura. A identificação das cepas isoladas foi realizada por meio de testes morfológicos e bioquímicos, como: testes de catalase, coagulase, hemólise, DNAse, termonuclease, produção de acetoína (VP) e metabolismo de carboidratos (glicose, maltose e manitol). Das 77 cepas coagulase positivas foram...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterotoxinas; Staphylococcus; Alimentos; Leite.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612006000100007
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Enteroxigenic Staphylococcus spp. and other microbial contaminants during production of Canastra cheese, Brazil BJM
Borelli,Beatriz M.; Ferreira,Elaine G.; Lacerda,Inayara C.A.; Santos,Deise A.; Carmo,Luiz S.; Dias,Ricardo S.; Silva,Maria Crisolita C.; Rosa,Carlos A..
Canastra cheese is produced from raw cow's milk, and it is made at the farmhouse level using artisanal procedures and natural starters. The aim of this work was to determine the main hygienic-sanitary indicators and enterotoxigenic staphylococcal strains present during the manufacturing of traditional cheese of Serra da Canastra region, Minas Gerais state, Brazil. Samples from 10 farms were studied, and they included: water employed in the process, raw milk, natural starters, cheese curd before salting and cheese after five days of ripening. All water samples exhibited faecal coliform contamination above the maximum acceptable value recommended by Brazilian standards. Pseudomonas aeruginosa and sulfite-reducing clostridia were also isolated from the water...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artisanal cheese; Coliforms; Staphylococcus; Enterotoxin.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400026
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Habituation of enterotoxigenic Staphylococcus aureus to Origanum vulgare L. essential oil does not induce direct-tolerance and cross-tolerance to salts and organic acids BJM
Tavares,Adassa Gama; Monte,Daniel Farias Marinho do; Albuquerque,Allan dos Reis; Sampaio,Fábio Correia; Magnani,Marciane; Siqueira Júnior,José Pinto de; Souza,Evandro Leite de.
Enterotoxigenic Staphylococcus aureus strains that were isolated from foods were investigated for their ability to develop direct-tolerance and cross-tolerance to sodium chloride (NaCl), potassium chloride (KCl), lactic acid (LA) and acetic acid (AA) after habituation in sublethal amounts (1/2 of the minimum inhibitory concentration - 1/2 MIC and 1/4 of the minimum inhibitory concentration - 1/4 MIC) of Origanum vulgare L. essential oil (OVEO). The habituation of S. aureus to 1/2 MIC and 1/4 MIC of OVEO did not induce direct-tolerance or cross-tolerance in the tested strains, as assessed by modulation of MIC values. Otherwise, exposing the strains to OVEO at sublethal concentrations maintained or increased the sensitivity of the cells to the tested...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus; Adaptation; Essential oil; Oregano.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300835
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Identification and antimicrobial susceptibility of Staphylococcus from home-treated peritoneal dialysis patients J. Venom. Anim. Toxins incl. Trop. Dis.
Batalha,JEN; Cunha,MLRS; Montelli,AC; Barreti,P; Caramori,JCT.
Staphylococcus aureus is the main agent of infections during peritoneal dialysis (PD). The presence of S. aureus in the nasal cavity has been extensively studied and suggested as a risk factor of dialysis-related infections, whereas coagulase-negative Staphylococcus (CNS) species are frequently considered part of the normal human microbiota. The aim of this study was to identify Staphylococcus in the nasal cavity, pericatheter skin and peritoneal effluent from PD patients, as well as to evaluate the antimicrobial activity evolution in vitro. Thirty-two chronic PD patients were observed during 12 months and had nasal and pericatheter skin samples collected for culture. When peritonitis was detected, samples were also collected from the peritoneal effluent...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peritoneal dialysis (PD); Peritonitis; Risk factor; Staphylococcus; Coagulase-negative Staphylococcus (CNS).
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200005
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Identification of 129 Micrococcaceae strains isolated from food of animal origin ArchiMer
Delarras, C; Guichaoua, C.; Caprais, Marie-paule.
129 strains of Micrococcaceae were isolated from food of animal origin (minced meat, cakes with confectioner's custard) on Baird-Parker medium. 120 were Staphylococcus and 9 Micrococcus, according to the ID 32 STAPH-System (1989). The results were analyzed using a computer program. 60% of these 120 Staphylococcus strains of animal origin were coagulase positive S. aureus (aurease+, Rapidec Staph technique). 46% were resistant to novobiocin (5 microg/ml), 13 to 44% were identified with negative discordant tests using this micromethod. 44% produced no acetoin, 35% had no urease and 15% no arginine dihydrolase. 48 Staphylococcus strains (40%) were identified as human coagulase negative Staphylococcus species (strains: 39; species: 9) or as animal species...
Tipo: Text Palavras-chave: Staphylococcus; Nitrofurantoin; Aurease; ID 32 STAPH; Numerical identification.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00071/18273/15838.pdf
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Identification of Staphylococcus spp. isolated during the ripening process of a traditional minas cheese Arq. Bras. Med. Vet. Zootec.
Borelli,B.M.; Lacerda,I.C.A.; Brandão,L.R.; Vianna,C.R.; Ferreira,M.C.; Gomes,F.C.O.; Carmo,L.S.; Heneine,L.G.D.; Rosa,C.A..
The population dynamics of Staphylococcus spp. was studied during the ripening of Canastra Minas cheese at three farms located in the State of Minas Gerais, Brazil. The presence of coagulase (coa), thermonuclease (nuc), and enterotoxin (sea, seb, sec, and sed) genes was investigated in Staphylococcus strains isolated during the 60-day cheese-ripening period. The presence of the staphylococcal enterotoxins A, C, and D was also investigated in the cheese samples. Cheese samples that were matured for 0, 7, 15, 30, and 45 days presented staphylococci counts from 10³ to 10(8)cfu/g. All isolates considered coagulase-positive by physiological tests had the coa gene. However, no association was observed between the results obtained with biochemical tests and those...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canastra Minas cheese; Staphylococcus; Enterotoxin.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000200028
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In vitro susceptibility to methicillin, vancomycin and linezolid of staphylococci isolated from bloodstream infections in eastern Turkey BJM
Tekin,Alicem; Dal,Tuba; Deveci,Özcan; Tekin,Recep; Özcan,Nida; Atmaca,Selahattin; Dayan,Saim.
Staphylococcus species are one of the major causes of bacterial bloodstream infections. Multi-resistant staphylococci infections are major therapeutic problems. This study was aimed to detect methicillin, linezolid and vancomycin susceptibilities of Staphylococcus isolates. A total of 870 Staphylococcus strains isolated from blood cultures of hospitalized patients with BSI. Antimicrobial susceptibilities of methicillin, linezolid and vancomycin were detected according to the Clinical and Laboratory Standards Institute (CLSI). A total of 771 (88.6%) isolates were coagulase-negative staphylococci (CoNS). 700 (80.5%) isolates were methicillin-resistant (MR) and 170 (19.5%) were methicillin-susceptible (MS). All the MS isolates were also susceptible to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus; Methicillin; Linezolid; Vancomycin.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300010
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Limitações da técnica de isolamento e enumeração de Staphylococcus aureus. Infoteca-e
NASCIMENTO, M. da G. F. do; CORBIA, A. C. G.; NASCIMENTO, E. R. do.
Tipo: Folhetos Palavras-chave: Alimentos; Minas frescal; Contaminação; Queijo; Staphylococcus.
Ano: 2001 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/415514
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Marine bacteria from the Gulf of California with antimicrofouling activity against colonizing bacteria and microalgae ArchiMer
Sanchez-rodriguez, Diana Elizabeth; Ortiz-aguirre, Ismael; Aguila-ramirez, Ruth Noemi; Guadalupe Rico-virgen, Erika; Gonzalez-acosta, Barbara; Hellio, Claire.
One way of reducing the input of pollutants into the marine environment is to enforce the use of non-toxic antifouling paints in marine protected areas. Thus, the purpose of this study was to detect marine microorganisms that secrete inhibitory substances against bacteria and microalgae to avoid biofouling on manmade structures in La Paz bay, B.C.S., Mexico. The inhibitory potential of 125 bacteria was evaluated against biofilm-forming bacteria. Crude extracts were obtained with methanol and ethyl acetate from 16 bacterial strains that exhibited antagonistic and antibacterial activity in a preliminary screening. Antibacterial and antimicroalgal assays were performed using crude extracts, the minimum inhibitory concentration (MIC) was determined. The...
Tipo: Text Palavras-chave: Epibionts; Extracts; Shewanella; Staphylococcus; Biofouling; Antifouling.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00616/72762/72271.pdf
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Mastite subclínica por Staphylococcus coagulase negativa em ovinos de corte Arq. Bras. Med. Vet. Zootec.
Tejada,T.S.; Silva,D.T.; Dias,P.A.; Conceição,R.C.S.; Müller Neto,H.; Timm,C.D..
Microorganisms that cause mastitis were isolated in a herd of sheep and their sensitivity to antimicrobials was tested. Twenty-one sheep had their mammary glands monitored during lactation. Eighty-eight milk sample from CMT-positive sheep were analyzed in order to isolate the etiologic agent of mastitis. Coagulase negative Staphylococcus were isolated from 29.5% of samples. Ampicillin and penicillin were the antimicrobials with higher number of resistant strains. The importance of coagulase negative staphylococci has been demonstrated, especially S. lentus and S. sciuri as a cause of ovine mastitis.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Coagulase negativa; Mastite; Ovinos; Staphylococcus.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400039
Registros recuperados: 37
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