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Classification of Bovine Follicles Based on the Concentrations of Steroids, Glucose and Lactate in Follicular Fluid and the Status of Accompanying Follicles OAK
NISHIMOTO, Hiromi; HAMANO, Seizo; HILL, Glen Alan; MIYAMOTO, Akio; TETSUKA, Masafumi; 宮本, 明夫; 手塚, 雅文.
A simple and clear means to identify the physiological status of follicles is essential for study of follicular biology. In the present study, we verified a novel classification procedure based on analysis of the follicular population and glucose concentration in follicular fluid (FF) as an alternative method to classify bovine follicles. Paired ovaries were collected from heifers, and the number of follicles and stage of the CL were recorded. Follicles were initially divided into the following 3 groups according to diameter and the ratio of E2 and P4 (E/P): E2 active (E-A: E/P≥1), E2 inactive (E-I: E/P<1, ≥8.5 mm) and small follicles (E/P<1, <8.5 mm). E-A follicles were easily identified as E2-rich dominant follicles and were further classified...
Palavras-chave: Bovine; Follicular fluid; Follicle classification; Glucose; Lactate; Steroid.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2828
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Effect of genistein-enriched diets on the endocrine process of gametogenesis and on reproduction efficiency of the rainbow trout Oncorhynchus mykiss ArchiMer
Bennetau Pelissero, Catherine; Breton, Bernard; Bennetau, Bernard; Corraze, Genevieve; Le Menn, Françoise; Davail Cuisset, Blandine; Helou, Chantal; Kaushik, Sadasivam.
Three practical diets were formulated to contain 0, 500, or 1000 ppm genistein. The three diets were distributed for 1 year to groups of rainbow trout undergoing their first gametogenesis and until spawning. Growth performance of rainbow trout was not affected by dietary treatments. Plasma cholesterol levels were equivalent between groups. In males, a slight but constant induction of vitellogenin (VTG) synthesis and a decrease in testosterone levels were observed. A slight decrease in plasma levels of beta FSH and beta LH was noticed at the end of spermatogenesis in the male fish fed a diet with 500 ppm (genistein) (from 2.16 +/- 0.39 to 1.47 +/- 0.23 for beta FSH and from 0.44 +/- 0.09 to 0.31 +/- 0.09 for beta LH). There was a significantly reduced 17...
Tipo: Text Palavras-chave: Diet; Reproduction; GTH; Vitellogenin; Steroid; Trout; Genistein.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-738.pdf
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Glucocorticoid susceptibility and in vivo ABCB1 activity differ in murine B cell subsets Anais da ABC (AABC)
COSTA,KELLI M. DA; VALENTE,RAPHAEL C.; SILVA,JOYLE M.C. DA; PAIVA,LUCIANA S. DE; RUMJANEK,VIVIAN M..
Abstract Glucocorticoids are produced and released by the adrenal gland and become elevated in response to stress. Although glucocorticoids are well known for their immunosuppressive effects, less is known about their effects on B cells. ABCB1 is an efflux pump expressed in both cancer and normal cells, modulating the gradient of various metabolites, including hydrocortisone. Our goal was to evaluate the effect of this glucocorticoid on murine B cell differentiation and whether sensitivity to hydrocortisone could be related to ABCB1 activity in vivo. C57BL/6 mice received one or three consecutive i.p. injections of hydrocortisone (70, 140 and 200 mg/kg/day). ABCB1 activity was evaluated via the rhodamine-123 transport and inhibited by cyclosporin A in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ABC transporters; B cells; Immunoregulation; Steroid.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000603081
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Protective effects of phosphodiesterase inhibitors on lung function and remodeling in a murine model of chronic asthma BJMBR
Campos,H.S.; Xisto,D.G.; Oliveira,M.B.G.; Teixeira,I.; Negri,E.M.; Mauad,T.; Carnielli,D.; Lima,L.M.; Barreiro,E.J.; Faffe,D.S.; Zin,W.A.; Lapa e Silva,J.R.; Rocco,P.R.M..
The aim of the present study was to compare the efficacy of a novel phosphodiesterase 4 and 5 inhibitor, LASSBio596, with that of dexamethasone in a murine model of chronic asthma. Lung mechanics (airway resistance, viscoelastic pressure, and static elastance), histology, and airway and lung parenchyma remodeling (quantitative analysis of collagen and elastic fiber) were analyzed. Thirty-three BALB/c mice were randomly assigned to four groups. In the asthma group (N = 9), mice were immunized with 10 µg ovalbumin (OVA, ip) on 7 alternate days, and after day 40 they were challenged with three intratracheal instillations of 20 µg OVA at 3-day intervals. Control mice (N = 8) received saline under the same protocol. In the dexamethasone (N = 8) and LASSBio596...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Asthma; Lung function; Steroid; Phosphodiesterase inhibitors; BALB/c mice.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200016
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