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Aislamiento e identificación de bacterias ácido lácticas con potencial probiótico en bovinos Holstein. Colegio de Postgraduados
Caballero Cervantes, Yesenia.
El principal problema de salud en becerras de reemplazo son las diarreas provocadas por Escherichia coli enterotóxica. El uso de microorganismos pertenecientes al grupo de bacterias ácido lácticas (BAL) en la etapa de lactancia es benéfico para combatir la incidencia de diarreas e incrementar la ganancia de peso. Sin embargo, se ha reportado inconsistencia en los efectos benéficos de los productos comerciales. Por lo anterior, el objetivo de este estudio fue aislar y seleccionar BAL con potencial probiótico de mucosa oral de becerros lactantes, calostro y leche de vacas Holstein que puedan ser consideradas para el suministro de becerras en México. Para ello, se realizó el aislamiento en caldo Man Rogosa Sharp (MRS) y en agar MRS para la obtención de...
Palavras-chave: Becerras; Lactobacillus; Cepas; Resistencia; Heifers; Strains; Resistance; Ganadería; Maestría.
Ano: 2014 URL: http://hdl.handle.net/10521/2358
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Análise genética de três gerações de tilápia do Nilo (linhagem GIFT) utilizando o marcador RAPD - doi: 10.4025/actascianimsci.v33i2.9202 Animal Sciences
Oliveira, Sheila Nogueira; Universidade Estadual de Maringa; Ribeiro, Ricardo Pereira; Universidade Estadual de Maringa; Lopera, Nelson Mauricio; Universidade Estadual de Maringa; Candioto, Fernanda Braz; Universidade Estadual de Maringa; Resende, Emiko Kawakami; EMBRAPA; Legat, Angela Puchinik; EMBRAPA.
O objetivo deste trabalho foi analisar a diversidade genética de três gerações da tilápia GIFT do Programa de Melhoramento Genético da Universidade Estadual de Maringá, utilizando o marcador RAPD. Foram utilizadas 180 amostras de nadadeira. Os sete primers utilizados produziram 91 fragmentos, dos quais 98,9% foram polimórficos. Observaram-se diferenças (p < 0,05) na frequência de 64 fragmentos, dos quais dez foram excluídos na G2. Os resultados de variabilidade genética estimados pelo índice de Shannon (G0: 0,430; G1: 0,469 e G2: 0,420) e pela porcentagem de fragmentos polimórficos (G0: 90,1%; G1: 94,5% e G2: 86,8%) indicaram alta variabilidade genética intrapopulacional. Resultados esses que corroborando com os preceitos de diversidade genética de Nei...
Tipo: Analise laboratorial Palavras-chave: Produção Animal diversidade genética; Genetic Improvement of Farmed Tilapias; Linhagens; Melhoramento genético; Oreochromis niloticus Piscicultura genetic diversity; Genetic Improvement of Farmed Tilapias; Strains; Genetic improvement; Oreochromis niloticus.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/9202
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Caracterização fisiológica e comparação de leveduras Saccharomyces e não-Saccharomyces na presença de diferentes inibidores presentes no hidrolisado lignocelulósico. Repositório Alice
SOARES, C. E. V. F..
Resumo: Várias espécies de leveduras tem sido avaliadas para a produção de combustíveis e produtos químicos a partir de açúcares presentes na biomassa lignocelulósica. O hidrolisado lignocelulósico contém açúcares e também compostos que inibem o metabolismo microbiano, como ácidos orgânicos, furaldeídos e compostos fenólicos. Compreender a resposta de leveduras a esses compostos inibitórios é importante para o desenvolvimento de bioprocessos. Nesse contexto, o objetivo geral desse trabalho foi identificar novas linhagens de leveduras capazes de metabolizar xilose e caracterizar a resposta fisiológica de diferentes espécies em termos de tolerância a inibidores presentes em hidrolisados lignocelulósicos. Inicialmente, as linhagens de leveduras JA1 e JA9,...
Tipo: Teses Palavras-chave: Leveduras; Xilose; Tolerância; Não-Saccharomyces; Hidrolisado; Inibidores; Saccharomyces; Enzyme inhibitors; Lignocellulose; Hydrolysates; Strains; Xylose.
Ano: 2021 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137344
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Comparison of the Quality Adjusting of Nonlinear Models for Organs, Carcass and Body Components in Meat-Type (Coturnix Coturnix Coturnix) and Laying-Type (Coturnix Coturnix Japonica) Quail Rev. Bras. Ciênc. Avic.
Grieser,DO; Furlan,AC; Ribeiro,PM; Zancanela,V; Del Vesco,AP; Gasparino,E; Zardin,AMSO; Marcato,SM.
ABSTRACT The objective of this study was to evaluate the adjustment quality of nonlinear models to data organs growth, carcass and body components of meat-type (Coturnix coturnix coturnix) and japanese laying-type (Coturnix coturnix japonica) quail. A total of 1350 quails from one to 42 d old were distributed in a completely randomized design, with five replicates each. To determine the organs growth (gut, heart, liver and gizzard), carcass and body components (wing, thigh and drumstick, back and breast), two quails per repetition were slaughtered weekly. The data were evaluated in function of different nonlinear models (Logistical, Brody, Richards, Von Bertalanffy and Gompertz). All models studied adjusted the data, differing in adjustment quality. Brody...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gompertz equation; Growth parameters; Breast; Number of interaction; Strains.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000400701
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Crescimento micelial in vitro de cinco linhagens de Agaricus bisporus submetidas a diferentes condições de temperatura - DOI: 10.4025/actasciagron.v32i1.333 Agronomy
Andrade, Meire Cristina Nogueira de; INPA; Chavari, João Lucas; UNESP; Minhoni, Marli Teixeira de Almeida; UNESP; Zied, Diego Cunha; UNESP.
Avaliou-se o crescimento micelial in vitro das linhagens ABI-05/03, ABI-06/04, ABI-04/02, ABI-06/05 e ABI-01/01 de Agaricus bisporus em meios de cultura sólidos à base de composto. As avaliações foram realizadas por meio de medições de quatro diâmetros das colônias, a cada 48h, durante 12 dias de incubação, no escuro, a 20 e 25&ordm;C. O delineamento experimental foi o de blocos casualizados, com uso do teste de Tukey para a comparação das médias. Com base nos resultados obtidos, verificou-se que: o crescimento micelial de A. bisporus é influenciado pela temperatura de incubação; a temperatura de 25&ordm;C foi mais favorável para o crescimento micelial de todas as linhagens de A. bisporus; na temperatura de 20&ordm;C, o melhor crescimento foi...
Palavras-chave: 5.01.00.00-9 Agronomia champignon; Micélio; Cogumelo; Crescimento micelial; Linhagens champignon; Mycelium; Mushroom; Mycelium growth; Strains.
Ano: 2010 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/333
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Digital monitoring of mycelium growth kinetics and vigor of shiitake (Lentinula edodes (Berk.) Pegler) on agar medium BJM
Montini,Renato Mamede de Castro; Passos,José Raimundo de Souza; Eira,Augusto Ferreira da.
The mycelium growth kinetics and vigor of shiitake (Lentinula edodes (Berk.) Pegler) strains LE 96/17, LE 98/51, LE 98/53, and LE 98/56 were studied under different agar medium compositions. The strains were from the mycological collection of the Módulo de Cogumelos, Faculdade de Ciências Agronômicas, Unesp-Botucatu, Brazil. Mycelium fragments from stock cultures were transferred to Petri dishes with Sawdust extract-Dextrose-Agar medium. The area of growth and vigor (density) of the mycelia were daily recorded with a digital camera, during incubation, until the complete colonization of the Petri dish. The images were analyzed by the freeware UTHSCSA ImageTool, v. 2.0, developed by the University of Texas Health Science Center, San Antonio. The kinetics of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lentinula edodes; Shiitake; Substrate; Mycelium growth; Strains.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100017
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Dynamics of the chemical composition and productivity of composts for the cultivation of Agaricus bisporus strains BJM
Andrade,Meire Cristina Nogueira de; Jesus,João Paulo Furlan de; Vieira,Fabrício Rocha; Viana,Sthefany Rodrigues Fernandes; Spoto,Marta Helena Fillet; Minhoni,Marli Teixeira de Almeida.
Two compost formulations based on oat straw (Avena sativa) and brachiaria (Brachiaria sp.) were tested for the cultivation of three Agaricus bisporus strains (ABI-07/06, ABI-05/03, and PB-1). The experimental design was a 2 x 3 factorial scheme (composts x strains) with 6 treatments and 8 repetitions (boxes containing 12 kg of compost). The chemical characterization of the compost (humidity, organic matter, carbon, nitrogen, pH, raw protein, ethereal extract, fibers, ash, cellulose, hemicellulose, and lignin) before and after the cultivation of A. bisporus and the production (basidiomata mass, productivity, and biological efficiency) were evaluated. Data were submitted to variance analysis, and averages were compared by means of the Tukey's test. According...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agaricus bisporus; Productivity; Strains; Compost; Nutritional value.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400016
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Effect of gamma irradiation on the nutritional quality of Agaricus bisporus strains cultivated in different composts Anais da ABC (AABC)
ANDRADE,MEIRE C.N.; JESUS,JOÃO P.F.; VIEIRA,FABRÍCIO R.; VIANA,STHEFANY R.F.; SPOTO,MARTA H.F.; MINHONI,MARLI T.A..
The effect of irradiation doses (0, 125, 250 and 500 Gy) on the nutritional quality of A. bisporus mushrooms (strains ABI-07/06, ABI-05/03 and PB-1) cultivated in composts based on oat straw (Avena sativa) and brachiaria (Brachiaria sp.) was evaluated. The experimental design was 4 x 3 x 2 factorial scheme (irradiation doses x strains x composts), with 24 treatments, consisting of two repetitions each, totaling 48 experimental units (samples of mushrooms). The samples were irradiated in Cobalt-60 irradiator, model Gammacell 220 kGy, with dose rate of 0.740 kGy h–1, according to the treatments proposed. Subsequently, the control (unirradiated) and the other treatments were maintained at 4±1°C and 90% RH in a climatic chamber for carrying out the chemical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agaricus bisporus; Productivity; Strains; Compost; Nutritional value.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000200897
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Elite Bradyrhizobium strains boost biological nitrogen fxation and peanut yield in tropical drylands. Repositório Alice
JOVINO, R. S.; SILVA, T. R. da S.; RODRIGUES, R. T.; CARVALHO, J. R. de S.; CUNHA, J. B. de A.; LIMA, L. M. de; SANTOS, R. C. dos; SANTOS, C. E. de R. e S.; RIBEIRO, P. R. de A.; FREITAS, A. D. S. de; MARTINS, L. M. V.; FERNANDES-JÚNIOR, P. I..
Peanut (Arachis hypogaea L.) is an important crop for the family-based systems in the tropics, mainly in Brazil. In the Brazilian drylands, peanuts are cropped in low technological systems, and cheap and efcient technologies are needed to improve crop yield and sustainability. Despite this importance, few data are available on selecting efcient peanut rhizobia in experiments under diferent edaphoclimatic conditions. This work evaluated the agronomic efciency and the biological nitrogen fxation (BNF) by two elite Bradyrhizobium strains under four diferent felds in the Brazilian semiarid region. We compared a new efcient strain Bradyrhizobium sp. ESA 123 with the reference strain B. elkanii SEMIA 6144, currently used in peanut rhizobial inoculants in Brazil....
Tipo: Artigo de periódico Palavras-chave: Nitrogênio; Amendoim; Semente; Seleção; Inoculante; Solo Árido; Seed inoculation; Strains; Arachis hypogaea subsp. hypogaea; Rhizobiales; Rhizobiaceae; Semiarid soils.
Ano: 2022 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1146324
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Endophytic colonization by Brazilian strains of Bacillus thuringiensis on cabbage seedlings grown in vitro. Repositório Alice
PRACA, L. B.; GOMES, A. C. M. M.; CABRAL, G. B.; MARTINS, E. S.; SUJII, E. R.; PONTES, R. G. M. S. de.
Tipo: Separatas Palavras-chave: Insecticidal activity; Colonization; Bacillus Thuringiensis; Cabbage; Strains.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/949937
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Evaluation of the performance of two strains of Nile tilapia (Oreochromis Niloticus) in mixed raising systems BABT
Neves,Patrícia Ribeiro; Ribeiro,Ricardo Pereira; Vargas,Lauro; Natali,Maria Raquel Marçal; Maehana,Káttia Regina; Marengoni,Nilton Garcia.
The aim of this study was to evaluate the productive performance of two strains of Nile tilapia (Oreochromis niloticus) in mixed raising systems. A total of 3600 fish-larvae species was used, 1800 belonging to Bouaké lineage, and 1800 to Chitralada. The experiment was carried out in three phases; Phase I in an incubator in 18 boxes, in which two treatments (Bouaké and Chitralada) were tested by using nine repetitions; Phases II and III were performed in 18 cement tanks with the same treatments. In phase I, regarding the final weight and gain of weight, Chitralada strain showed the highest final weight values. In phase II, Chitralada showed the highest final weight value when compared with Bouaké, and, considering the gain of weight, Bouaké obtained the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Performance; Strains; Oreochromis niloticus; Reproduction; Nile tilapia.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300012
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Extended serotyping scheme for <i>Vibrio anguillarum</i> with the definition and characterization of seven provisional O-serogroups OMA
Pedersen, K.; Grisez, L.; van Houdt, R.; Tiainen, T.; Ollevier, F.P.; Larsen, J.L..
The present paper summarizes the serotyping scheme of the fish pathogenic bacterium <i>Vibrio anguillarum</i> and defines seven additional O-serogroups. Strains, collected in our laboratories that were nontypable with antisera against the previously defined 16 O-serotypes, were used for generating new antisera and were characterized further by means of LPS profiles, Western blots, and serological reactions. On the basis of the results, it is suggested that the seven new O-serogroups are to be included in the existing serotyping system as serotypes O17-O<sub>2</sub>3. However, the existence of further <i>V. anguillarum</i> strains that were not typable with any of the 23 O-antisera suggested the existence of additional...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diseases; Fish diseases; Pathogenic bacteria; Pathogens; Serological studies; Serotypes; Strains; Vibrio anguillarum.
Ano: 1999 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=9525
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Fermentative behavior of native lactobacilli in goat milk and their survival under in vitro simulated gastrointestinal conditions. Repositório Alice
GALDINO, I. K. C. P. de O.; OLIVEIRA, M. M.; OLIVEIRA, A. T.; SILVA, G. M. da; OLIVEIRA, T. A. de; SANTOS, K. M. O. dos; EGITO, A. S. do; BURITIA, F. C. A..
In this study, goat fermented milk trials were prepared with six potentially probiotic autochthonous cultures Lactobacillus rhamnosus EM1107, Lactobacillus plantarum (CNPC001, CNPC002, CNPC003 and CNPC004) and Lactobacillus mucosae CNPC007in the presence and absence of a starter culture (Streptococcus thermophilus, ST) and in the presence and absence of inulin (IN) or oligofructose (FOS), consisting of 36 trials. The fermented milk without the starter culture only achieved a lactic acid content above 0.6 g/100 g after 24 h, thus requiring the presence of ST culture for a faster acidification. After the fermentation, three trials with the best fermentative performance and visual firmness (EM1107 + ST + IN, CNPC003 + ST + IN and CNPC007 + ST + IN) were...
Tipo: Artigo de periódico Palavras-chave: Fermented goat milk; Probiotic potential; Lactobacillus spp; Prebiotic; In vitro gastrointestinal resistance; Food technology; Lactic acid bacteria; Functional properties; Prebiotics; Strains; Inulin; Coculture; Lactobacillus plantarum; Lactobacillus rhamnosus; Lactobacillus casei; Viability.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1124541
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Growth, delayed fluorescence and pigment composition of four Prorocentrum minimum strains growing at two salinities Biol. Res.
BERDEN-ZRIMEC,MAJA; FLANDER-PUTRLE,VESNA; DRINOVEC,LUKA; ZRIMEC,ALEXIS; MONTI,MARINA.
Prorocentrum minimum is a potentially harmful and widely distributed marine dinoflagellate. Several P. minimum strains have already been studied, showing phylogenetical relations of strains isolated from the same geographical regions. Similarity among the strains was further examined on the basis of their physiology. Pigment composition and concentration, as well as delayed fluorescence (DF) decay kinetics and intensity, were measured in four P. minimum strains isolated from the Baltic and Adriatic Seas. The strains were grown at two salinities characteristic of the Baltic (8 PSU) and North Adriatic Seas (32 PSU). Strain differences in DF decay kinetics and growth did not always follow their genetic relations. While two strains showed similarities to the...
Tipo: Journal article Palavras-chave: Adriatic Sea; Baltic Sea; Delayed fluorescence; HPLC; Prorocentrum minimum; Strains.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100003
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Heterogeneity among Strains of Eimeria tenella Isolated from Bangladesh OAK
Karim, M. J.; Begum, N.; Khan, M. S. R..
Palavras-chave: Eimeria; Strains; Heterogeneity; Immunity; Precocious.
Ano: 1994 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/205
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MALDI-TOF MS Supplementary database for species identification employing the yeast diversity encountered on southern Brazil grapes. Repositório Alice
AGUSTINI, B. C.; SILVA, G. A. da; BOMFIM, T. M. B. B..
The study of grape microflora is of interest when autochthonous yeasts, which are related to typical wine characteristics, are intended to be used in winemaking. The election of matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) as the first method for yeast identification was based on its accuracy and rapidity compared to alternative laboratory protocols for identification. The aims of this study are to consolidate the MALDI-TOF MS Supplementary database for environmental yeasts already constructed, to expand it through the addition of standard spectra of not included yeast species, and to discuss the grape microflora encountered in Southern Brazil. A total of 358 strains, isolated from grape berries, were...
Tipo: Artigo de periódico Palavras-chave: MALDI-TOF MS spectra; Grape microflora; Candida californica; Zygoascus meyerae; Candida akabanensis; Candida azyma; Strains; Grapes; Hanseniaspora vineae.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102899
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Molecular variants of Grapevine rupestris stem pitting-associated virus infecting grapevines (Vitis spp.) in Brazil Ciência Rural
Fajardo,Thor Vinícius Martins; Kin,Aléxis Cardama; Nickel,Osmar.
ABSTRACT: Grapevine rupestris stem pitting-associated virus (GRSPaV) is one of the most common viruses of grapevine. It is involved in the graft-transmissible disease rupestris stem pitting of the rugose wood complex. The objective of the research was to perform the molecular characterization of the coat protein (CP) gene of sixteen Brazilian GRSPaV isolates aiming to determine the occurrence of molecular variants (strains) of this virus. Nine grapevine samples were evaluated, from which dsRNA was extracted. Nucleotide sequences were generated by Next generation sequencing (NGS). Fifteen complete sequences of the GRSPaV CP gene were obtained and phylogenetically analyzed. Multiple alignments of the sequences showed identities of nucleotides ranging from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: GRSPaV; Foveavirus; Strains; Lineages; Genetic variability.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001100153
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Plant growth promotion by four species of the genus Burkhoderia. Repositório Alice
ALVES, G. C.; MACEDO, A. V. de M.; REIS JUNIOR, F. B. dos; URQUIAGA, S.; REIS, V. M..
Abstract: Background and aims: The genus Burkholderia is highly diverse and may mediate plant growth via several mechanisms. The current study evaluates the role of strains from four Burkholderia species in maize growth promotion. Methods: Twenty three strains of diazotrophic Burkholderia species were applied to two maize genotypes (SHS5050 and BRS4157) under greenhouse and field conditions. Strains were tested for growth promotion in greenhouse during 40 days. Strain 41 from B. silvatlantica was used under field conditions. Parameters evaluated: biomass accumulation, N and root measurements in pots and grain yield, N % and N in the grains under field conditions. Results: Eleven strains were evaluated under soil substrate. The inoculation of the hybrid...
Tipo: Separatas Palavras-chave: Nytrogen fixation.; Fixação de nitrogênio; Milho; Zea Mays.; Burkholderia; Strains; Corn..
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1036050
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Propuesta de una nueva metodología para diferenciar las cepas de Yersinia ruckeri de las de Hafnia alvei OceanDocs
Aguilera, Y.; Martínez, M.T.; Silveira, R..
El objetivo del estudio fue revisar la identificación de dos posibles cepas de Yersinia ruckeri aisladas de diferentes epizootias, diagnosticadas como septicemia bacteriana en 2009 en tilapia cultivada en jaulas, para esclarecer su papel en el cuadro clínico, ya que en los animales afectados no se observaron los signos característicos de la yersiniosis. Se realizó una amplia batería de pruebas bioquímicas mediante técnicas convencionales. La incubación de la motilidad, del Citrato de Simons y del Voges Proskauer, se hizo por duplicado, una réplica se incubó a 25 ºC y la otra, a 37 ºC, lo que permitió identificar a las cepas como Hafnia alvei, que no es un patógeno reconocido de los organismos acuáticos. Se propone un esquema para diferenciar las cepas de...
Tipo: Journal Contribution Palavras-chave: Strains; Biochemical analysis.
Ano: 2011 URL: http://hdl.handle.net/1834/4144
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Separation of Escherichia coli from natural samples for identification of sources and microcosm inoculation. Repositório Alice
CARNEIRO, M. T.; PEREZ, D. V.; FEITOSA, R. C.; WASSERMAN, J. C..
Obtaining uncultured Escherichia coli from natural waters is an important step in the study of microbes in the environment, which are critical for bacterial decay and microbial source tracking. The quality of the samples used can influence the assays, because high contaminant concentrations, differing cell ages, and physiologic states can impair results. The proposed separation is based on a three-step filtration method applied to replicates of seven samples from a sewage plant affluent, collected in different periods. Aliquots of the leachate were inoculated into microcosms, aiming to observe the cultivability of the cells. The assay resulted in colimetry values ranging between 104 and 105 cells. In the leachate, averages of 1.05% of total coliforms and...
Tipo: Artigo de periódico Palavras-chave: Escherichia Coli; Águas Residuais; Rastreamento; Bactéria; Wastewater; Strains.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125143
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