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López Garrido, Serafín Jacobo. |
El objetivo del presente estudio fue obtener un cultivo puro de la bacteria ruminal Streptococcus bovis a partir de líquido ruminal de ovinos alimentados con una dieta alta en sorgo (70 % en base seca). Además, se analizó su actividad fermentativa (producción de AGV y lactato) y el proceso de liofilizado como método de conservación, y se realizaron ensayos para expresar su bacteriófago. Para el aislamiento se usó un medio comercial para Streptococcus spp. (Merck) preparado bajo anaerobiosis. Para la identificación se usó el sistema API 20 Strep, reacción catalasa, morfología celular y tinción de Gram. Con la prueba API 20 Strep se comprobó que la bacteria aislada pertenece a la especie S. bovis. Esta bacteria tiene forma esférica a ovoide, de 0.8... |
Tipo: Tesis |
Palavras-chave: Rumen; Streptococcus bovis; Aislamiento; Iiofilización; Bacteriófago; Doctorado; Ganadería; Isolation; Lyophilization; Bacteriophage. |
Ano: 2008 |
URL: http://hdl.handle.net/10521/1402 |
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Hörner,Rosmari; Salla,Adenilde; Oliveira,Loiva Otonelli de; Forno,Nara Lucia Frasson Dal; Righi,Roselene Alves; Domingues,Vanessa Oliveira; Rigatti,Fabiane; Mayer,Letícia Eichstaedt. |
Spontaneous bacterial peritonitis (SBP) is a frequent and severe complication that occurs in patient with cirrhosis and ascites. It occurs in 10% to 30% of patients admitted to hospital. The organisms that cause SBP are predominantly enteric. Escherichia coli is the most frequent recovered pathogen, and Gram-positive bacteria, mainly Staphylococcus spp., are being considered an emerging causative agent of SBP. Streptococcus bovis that may be found as part of the commensal bowel flora in about 10% of healthy adults constitute an uncommon cause of peritonitis that was first reported in 1994. We describe the first case of SBP at the University Hospital of Santa Maria (HUSM) caused by S. bovis, resistant to the antibiotics erythromycin and clindamycin... |
Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Peritonitis; Streptococcus bovis; Cirrhosis; Ascites. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000300017 |
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Arcuri,Pedro Braga; Odenyo,Agnes Awino; Arcuri,Edna Froeder; Ribeiro,Marlice Teixeira; Guimarães,Marta Fonseca Martins; Carneiro,Jailton da Costa. |
The objective of this work was to isolate and characterize tannin-tolerant ruminal bacteria from crossbred Holstein x Zebu cows fed a chopped mixture of elephant grass (Pennisetum purpureum), young stems of "angico-vermelho" (Parapiptadenia rigida), and banana tree (Musa sp.) leaves. A total of 117 bacteria strains were isolated from enrichment cultures of rumen microflora in medium containing tannin extracts. Of these, 11 isolates were able to tolerate up to 3 g L-1 of tannins. Classical characterization procedures indicated that different morphological and physiological groups were represented. Restriction fragments profiles using Alu1 and Taq1 of 1,450 bp PCR products from the 16S rRNA gene grouped the 11 isolates into types I to VI. Sequencing of 16S... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Butyrivibrio fibrisolvens; Streptococcus bovis; Anti-nutritional polyphenolics; Ruminant nutrition. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000300007 |
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