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Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus BJM
Barrientos,Leticia; Herrera,Christian L.; Montenegro,Gloria; Ortega,Ximena; Veloz,Jorge; Alvear,Marysol; Cuevas,Alejandro; Saavedra,Nicolás; Salazar,Luis A..
Propolis is a non-toxic natural substance with multiple pharmacological properties including anticancer, antioxidant, fungicidal, antibacterial, antiviral, and anti-inflammatory among others. The aim of this study was to determine the chemical and botanical characterization of Chilean propolis samples and to evaluate their biological activity against the cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. Twenty propolis samples were obtained from beekeeping producers from the central and southern regions of Chile. The botanical profile was determined by palynological analysis. Total phenolic contents were determined using colorimetric assays. Reverse phase HPLC and HPLC-MS were used to determine the chemical composition. The minimum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Propolis; Botanical characterization; Chemical characterization; Streptococcus mutans; Streptococcus sobrinus.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200038
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In vitro bacterial plaque suppression and recolonization by S. mutans and S. sobrinus BJM
Pereira,Cássio Vicente; Pereira,Luciano José; Gonçalves,Reginaldo Bruno; Höfling,José Francisco.
The in vitro study of the interactions between S. mutans and S. sobrinus is important to determine the role of these microorganisms in the formation of biofilms on dental structures and their potential to induce carious lesions. The objective of this research was to study the suppression of bacterial plaque formation and its recolonization by rifampycin-resistant S.mutans and streptomycin-resistant S. sobrinus. To study the competitive relationship between these species, previously standardized strains were incubated in media containing different fermentable carbohydrates. At determined time intervals, samples were collected from mixed cultures of S. mutans and S. sobrinus, diluted and plated on BHI-agar containing rifampycin or streptomycin to determine...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptococcus mutans; Streptococcus sobrinus; Suppression.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100004
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