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Brewer's spent grain and corn steep liquor as alternative culture medium substrates for proteinase production by Streptomyces malaysiensis AMT-3 BJM
Nascimento,Rodrigo Pires do; Alves Junior,Nelson; Coelho,Rosalie Reed Rodrigues.
Brewer's spent grain and corn steep liquor or yeast extract were used as the sole organic forms for proteinase production by Streptomyces malaysiensis in submerged fermentation. The influence of the C and N concentrations, as well as the incubation periods, were assessed. Eight proteolytic bands were detected through gelatin-gel-electrophoresis in the various extracts obtained from the different media and after different incubation periods, with apparent molecular masses of 20, 35, 43, 50, 70, 100, 116 and 212 kDa. The results obtained suggest an opportunity for exploring this alternative strategy for proteinases production by actinomycetes, using BSG and CSL as economically feasible substrates.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces malaysiensis; Brewer's spent grains; Corn steep liquor; Proteinases; Submerged fermentation.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400020
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Cashew apple juice as microbial cultivation medium for non-immunogenic hyaluronic acid production BJM
Oliveira,Adriano H.; Ogrodowski,Cristiane C.; Macedo,André C. de; Santana,Maria Helena A.; Gonçalves,Luciana R.B..
In this work, natural cashew apple juice was used as cultivation medium as an alternative to substitute brain heart infusion medium. The effect of aeration and juice supplementation with yeast extract on the production of hyaluronic acid in batch fermentation was also investigated. Similar levels of cell mass were obtained in inoculum using cashew apple juice supplemented with yeast extract or the conventional brain heart infusion medium. Fermentation in Erlenmeyer flasks produced low biomass and hyaluronic acid concentrations. The hyaluronic acid concentration and viscosity increased from 0.15 g/L and 3.87 cP (no aeration or medium supplementation) to 1.76 g/L and 107 cP, when aeration (2 vvm) and 60 g/L of yeast extract were used. The results suggest the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hyaluronic acid; Cashew apple juice; Submerged fermentation.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400011
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Comparative study of a new alkaline L-methioninase production by Aspergillus ustus AUMC 10151 in submerged and solid-state fermentation BABT
Abu-Tahon,Medhat Ahmed; Isaac,George Saad.
Twenty four fungal species were screened for their ability to produce alkaline L-methioninase on methionine-glucose liquid medium. Aspergillus ustus AUMC 10151 displayed the highest yield of enzyme (10.8 U/mg protein), followed by A. ochraceus and Fusarium proliferatum. Upon optimization of the submerged fermentation (SmF)conditions, the maximum enzyme yield (18.23 U/mg protein) was obtained on a medium containing L-methionine (0.5%), sucrose (0.95%), KH2PO4 (0.1%) and 175 rpm. Seven agro-industrial by-products were screened as substrates for L-methioninase production under solid-state fermentation (SSF). Wheat bran resulted 38.1 U/mg protein, followed by rice bran (27.6 U/mg protein) and soya bean meal (26.6 U/mg protein). Maximum alkaline L-methioninase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: L-methioninase; Aspergillus ustus; Submerged fermentation; Solid state fermentation; Wheat bran.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100502
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Concentration, characterization and application of lipases from Sporidiobolus pararoseus strain BJM
Smaniotto,Alessandra; Skovronski,Aline; Rigo,Elisandra; Tsai,Siu Mui; Durrer,Ademir; Foltran,Lillian Liva; Paroul,Natália; Luccio,Marco Di; Oliveira,J. Vladimir; Oliveira,Débora de; Treichel,Helen.
Lipases produced by a newly isolated Sporidiobolus pararoseus strain have potential catalytic ability for esterification reactions. After production, the enzymatic extracts (conventional crude and precipitated, 'CC' and 'CP', and industrial crude and precipitated, 'IC' e 'IP') were partially characterized. The enzymes presented, in general, higher specificity for short chain alcohols and fatty acids. The precipitated extract showed a good thermal stability, higher than that for crude enzymatic extracts. The 'CC' and 'CP' enzymes presented high activities after exposure to pH 6.5 and 40 ºC. On the other hand, the 'IC' and 'IP' extracts kept their activities in a wide range of pH memory but presented preference for higher reaction temperatures. Preliminary...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sporidiobolus pararoseus; 'synthetic lipase'; Submerged fermentation; Enzyme characterization.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100043
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Critical process parameters optimization for hyperthermostable β amylase production by Bacillus subtilis DJ5 using response surface methodology - doi: 10.4025/actascibiolsci.v36i1.17427 Biological Sciences
Poddar, Abhijit; Bidhannagar College (govt. of West bengal); Ghara, Tushar Kanti; Bidhannagar College (govt. of West bengal); Jana, Subhas Chandra; Bidhannagar College (govt. of West bengal).
The combined effects of significant physical and chemical factors affect hyperthermostable β amylase production under submerged fermentation by Bacillus subtilis DJ5. The above was studied using the experimental design and response surface methodology. A 23 full-factorial central composite design was chosen to analyze interactions among three factors i.e. substrate concentration, medium pH and incubation temperature. The experimental data were fitted into a polynomial model for the yield of enzyme and an optimum level was arrived at with optimized conditions. Solving the coded values using Excel equation function indicated that maximum enzyme production is possible at a substrate concentration of 7.07 mg mL-1, pH 6.622 and temperature of 35.435°C. Such...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microbiology central composite design; Response surface plot; Contour plot; Submerged fermentation; Thermozymes Microbiology.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/17427
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Culture conditions for the production of a-galactosidase by Aspergillus parasiticus MTCC-2796: a novel source Electron. J. Biotechnol.
Shivam,Kumar; Tripathi,Chandra Prakash Mani; Mishra,Sarad Kumar.
Aspergillus parasiticus microbial type culture collection (MTCC)-2796, a new source of a-galactosidase is an efficient producer of enzyme in basic medium under submerged fermentation conditions. Maximum a-galactosidase production (156.25 Uml-1) was obtained when the basic medium is supplemented with galactose (0.5% w/v) and raffinose (0.5% w/v) as carbon source and yeast extract as nitrogen source. Enzyme production was also enhanced considerably in the presence of wheat bran (1.0% w/v). Enzyme secretion was strongly inhibited by the presence of Hg2+, Cu2+, and Co2+ in the medium and to some extent by Zn2+ and Ni2+, while marginal increase in the enzyme production was observed when Mg2+ and Mn2+ were added in the medium. Among amino acids checked...
Tipo: Journal article Palavras-chave: Aspergillus parasiticus; A-galactosidase; Culture condition; Submerged fermentation.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400004
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Development of an L-Lysine Enriched Bran for Animal Nutrition via Submerged Fermentation by Corynebacterium glutamicum using Agroindustrial Substrates BABT
Letti Junior,Luiz Alberto; Letti,Gilberto Vinícius Melo; Soccol,Carlos Ricardo.
L-Lysine is an essential aminoacid added as supplement for animal feed. The aim of this work was to produce an L-Lysine enriched bran using Brazilian agroindustrial byproducts. Both the raw material costs and purification steps were minimized. Firstly, medium composition for the growth of Corynebacterium glutamicum ATCC 21799 was optimized targeting enhanced L-Lysine production - salt, vitamins and nitrogen sources concentrations were tested and selected. Next, UV mutant strains were generated and the best producers were used in formulated media using sugarcane molasses. It was reached a production of 9.3 g/L of L-Lysine with the optimized formulated media. This L-Lyisne rich broth was then impregnated and cyclically reimpregnated in pre-treated solid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: L-Lysine; Corynebacterium glutamicum; Submerged fermentation; Animal nutrition; Enriched bran.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100505
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Economic Analysis of Cellulase Production by Clostridium thermocellum in Solid State and Submerged Fermentation AgEcon
Zhuang, Jun; Marchant, Mary A.; Nokes, Sue; Strobel, Herbert.
Dependence on foreign oil remains a serious issue for the U.S. economy. Additionally, automobile emissions related to petroleum-based, fossil fuel has been cited as one source of environmental problems, such as global warming and reduced air quality. Using agricultural and forest biomass as a source for the biofuel ethanol industry, provides a partial solution by displacing some fossil fuels. However, the use of high cost enzymes as an input is a significant limitation for ethanol production. Economic analyses of cellulase enzyme production costs using solid state cultivation (SSC) are performed and compared to the traditional submerged fermentation (SmF) method. Results from this study indicate that the unit costs for the cellulase enzyme production are...
Tipo: Working or Discussion Paper Palavras-chave: Biomass; Enzyme production; Ethanol; Solid state fermentation; Submerged fermentation; Resource /Energy Economics and Policy.
Ano: 2004 URL: http://purl.umn.edu/31982
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Effect of vacuum and oven drying on the radical scavenging activity and nutritional contents of submerged fermented Maitake (Grifola frondosa) mycelia Ciênc. Tecnol. Aliment.
SIM,Kheng Yuen; LIEW,Jeng Young; DING,Xin Yi; CHOONG,Wan Shin; INTAN,Seri.
Abstract Maitake (Grifola frondosa) mycelia contain high dietary and medicinal values that have gained great attentions from consumers. Proper drying can preserve their bio-availabilities prior to subsequent processing or consumption. Pure Maitake (Grifola frondosa) strain was cultivated in Mushroom Complete Medium (MCM) using submerged fermentation for 14 days. Maitake mycelia were harvested and treated respectively by vacuum, oven drying treatments while the fresh mycelia were assigned as control. All the samples were tested for Total Phenolic Content, DPPH radical scavenging assays and nutritional contents. Submerged fermentation produced mycelia biomass (1.3 ± 0.1 g/L) within 14 days of fermentation. Both the pH and reduced sugar content had decreased...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DPPH radical scavenging; Maitake mycelia; Submerged fermentation; Total soluble protein; Vacuum drying.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000500131
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Exploring fungal biodiversity in Brazil for natural colorants production. Repositório Alice
SAMPAIO, B. L.; MIDORIKAWA, G. E. O.; SILVA, C. A. L.; SANTOS, L. C. Z.; OLIVEIRA, P. A. de.
The objectives of this study were to select novel pigment-producing fungal strains, characterize the major colored metabolites in the selected crude extracts, and evaluate the extracts for their stability to light, pH, and temperature.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Fermentação submersa; Fungo; Biodiversidade; Fermentação; Fungi; Biodiversity; Anthraquinones; Submerged fermentation; Food coloring.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126349
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High cellulolytic activities in filamentous fungi isolated from an extreme oligotrophic subterranean environment (Catão cave) in Brazil Anais da ABC (AABC)
PAULA,CAIO C.P. DE; MONTOYA,QUIMI V.; MEIRELLES,LUCAS A.; FARINAS,CRISTIANE S.; RODRIGUES,ANDRÉ; SELEGHIM,MIRNA H.R..
Abstract Abstract: Isolation and screening of new fungal strains from extreme and understudied environments, such as caves, is a promising approach to find higher yields enzyme producers. Cellulolytic fungal strains isolated from a Brazilian cave were evaluated for their enzymatic production after submerged (SmF) and solid-state fermentation (SSF). After SmF, three strains were selected for their high enzymatic activities: Aspergillus ustus for endoglucanase (4.76 U/mg), Talaromyces bruneus for β-glucosidase (11.71 U/mg) and Aspergillus sp. (CBMAI 1926) for total cellulase (1.70 U/mg). After SSF, these strains, showed better yields compared to the reference strain Aspergillus niger 3T5B8. Aspergillus sp. (CBMAI 1926) stood out as a new species that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulases; Fungu’s cave; Solid state fermentation; Submerged fermentation; Subterranean environment.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500601
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Influence of initial pH of the growing medium on the activity, production and genes expression profiles of laccase of Pleurotus ostreatus in submerged fermentation Electron. J. Biotechnol.
Díaz,Rubén; Téllez-Téllez,Maura; Sánchez,Carmen; Bibbins-Martínez,Martha D; Díaz-Godínez,Gerardo; Soriano-Santos,Jorge.
Background: Enzymatic activity and laccase isoenzymes number of Pleurotus ostreatus grown in different pH values of the growing medium in submerged fermentation and incubated in buffer solutions of different initial pH values were determined. The expression profiles of five laccase genes (Lacc1, Lacc4, Lacc6, Lacc9 and Lacc10) in these cultures were also studied. Results: The highest laccases activity was obtained in cultures grown at initial pH of 4.5 and the lowest in cultures grown at initial pH of 8.5. Isoenzyme profiles were different in all the cases. Lacc1, Lacc4, Lacc6 and Lacc10 were expressed in all the cultures. Conclusions: The initial pH of the growing medium is an important factor for regulating the expression of laccase genes, having an...
Tipo: Journal article Palavras-chave: Laccases; Pleurotus ostreatus; Submerged fermentation; Zymogram.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400006
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Kinetics of high-Level of ß-glucosidase production by a 2-deoxyglucose-resistant mutant of Humicola lanuginosa in submerged fermentation BJM
Bokhari,Syed Ali Imran; Latif,Farooq; Rajoka,Muhammad Ibrahim.
A 2-deoxyglucose-resistant mutant (M7) of Humicola lanuginosa was obtained by exposing conidia to γ-rays and permitting expression in broth containing 0.6% 2-deoxyglucose (DG) and cellobiose (1%) before plating on DG esculin-ferric ammonium citrate agar medium from which colonies showing faster and bigger blackening zones were selected. Kinetic parameters for enhanced ß-glucosidase (BGL) synthesis by M7 were achieved when corncobs acted as the carbon source. The combination between corncobs and corn steep liquor was the best to support higher values of all product formation kinetic parameters. Effect of temperature on the kinetic and thermodynamic attributes of BGL production equilibrium in the wild organismand M7was studied using batch process at eight...
Tipo: Info:eu-repo/semantics/article Palavras-chave: SS-Glucosidase; Enthalpy; Entropy; Hemicellulose; Kinetics; Regulation; Submerged fermentation.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400024
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Lipase Production by Aspergillus niger C by Submerged Fermentation BABT
Lima,Laisy Garcia Ribeiro; Gonçalves,Márcia Monteiro Machado; Couri,Sonia; Melo,Verônica Ferreira; Sant’Ana,Gizele Cardoso Fontes; Costa,Antônio Carlos Augusto da.
Abstract This study aimed to evaluate the effects of variables on the process of lipases production by Aspergillus niger C by submerged fermentation (SmF). The production assays were performed in shake flasks for 72 hours at 150 rpm and 32°C. First, a fractional factorial design 25-1 (FFD) was carried out to evaluate the effect of the following process variables: sucrose, ammonium sulphate, soybean oil, yeast extract concentration and pH. After the selection of the variables that significantly influenced the lipase production, a central composite rotational design 22 (CCRD) was used, aiming to find the most favorable operational conditions. The selected assay condition (15.0 g.L-1 sucrose, 4.0 g.L-1 ammonium sulphate, 4.0 g.L-1 soybean oil and 1.0 g.L-1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipases; Submerged fermentation; Aspergillus nige; Experimental design..
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100214
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Lipid content and fatty acid composition of a marine-derived Trichoderma longibrachiatum strain cultured by agar surface and submerged fermentations ArchiMer
Ruiz, Nicolas; Dubois, Nolwenn; Wielgosz Collin, Gaetane; Du Pont, Thibaut; Berge, Jean-pascal; Pouchus, Yves-francois; Barnathan, Gilles.
A marine-related Trichoderma longibrachiatum strain isolated from mussels in a farming shellfish area was investigated for total lipid (TL) production, total lipid fatty acids (TLFA), and phospholipid fatty acids (PLFA). Fungal biomass was produced from two different marine-like culture ways, on agar surface and submerged fermentations (ASF and SmF, respectively), allowing useful comparisons. ASF produced a rather higher biomass amount than SmF with similar TL content. All fatty acids (FA), identified by gas chromatography-mass spectrometry (GC-MS), were ranged from 14 to 30 carbon atoms. Similar FA compositions were found in TL and phospholipids (PL) from biomasses obtained by both fermentation systems, including oleic (up to 15.3% of total FA mixture for...
Tipo: Text Palavras-chave: Conjugated fatty acids; Fatty acids; Lipids; Submerged fermentation; Agar surface fermentation; Trichoderma longibrachiatum.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-4199.pdf
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Localization and partial characterization of thermostable glucoamylase produced by newly isolated Thermomyces lanuginosus TO3 in submerged fermentation BABT
Gonçalves,Aline Zorzetto Lopes; Carvalho,Ana Flávia Azevedo; Silva,Roberto da; Gomes,Eleni.
Thermophilic Thermomyces lanuginosus strain TO3 was isolated from compost pile samples and was used for its ability to produce considerable glucoamylase activity when growing in liquid medium at 45ºC with starch as the sole carbon source. Enzyme productivity was high in submerged fermentation (SmF) with maximum activity of 13 U/mL after 168 h of fermentation. Higher quantities of glucose were released when the substrate for enzyme was soluble starch than maltose or maltooligosaccharides were used. The distribution of glucoamylase between the extracellular and cell-associated fractions varied according to fermentation time. Glucoamylase produced from T. lanuginosus TO3 had optimum activity at 65 ºC and good thermostability in the absence of substrate, with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucoamylase; Submerged fermentation; Thermomyces lanuginosus.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400024
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Microbial production of citric acid BABT
Vandenberghe,Luciana P. S; Soccol,Carlos R; Pandey,Ashok; Lebeault,Jean-Michel.
Citric acid is the most important organic acid produced in tonnage and is extensively used in food and pharmaceutical industries. It is produced mainly by submerged fermentation using Aspergillus niger or Candida sp. from different sources of carbohydrates, such as molasses and starch based media. However, other fermentation techniques, e.g. solid state fermentation and surface fermentation, and alternative sources of carbon such as agro-industrial residues have been intensively studied showing great perspective to its production. This paper reviews recent developments on citric acid production by presenting a brief summary of the subject, describing micro-organisms, production techniques, and substrates, etc.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citric acid; Aspergillus niger; Submerged fermentation; Solid state fermentation; Substrates.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300001
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Microbial rennet produced by Mucor miehei in solid-state and submerged fermentation BABT
Silveira,Guilherme Garcia da; Oliveira,Gustavo Monteiro de; Ribeiro,Eloizio Julio; Monti,Rubens; Contiero,Jonas.
The aim of this work was to study the effect of carbon and nitrogen sources in submerged fermentation, and the casein effect on solid-state fermentation on rennin production by Mucor miehei. Biomass peaks reached 6.7; 8.1 and 8 g/L and enzymatic production peaks of 1,066; 857 and 480 Soxhlet Units (S.U.) for glucose concentration of 18; 25 and 35 g/L respectively. Flasks with baffles showed biomass peaks of 6.7; 8.3 and 10 g/L and enzyme activity peaks of 648; 279 and 300 S.U. for the same glucose concentration. The values of 923 and 667 S.U. were obtained when corn steep liquor and Proflo (Cottonseed Nutrients from Traders ®) were used. In SSF system the enzymatic activities were 414, 264 and 167 S.U., when using HCl 0.2 N, 0.3 N and 0.4 N respectively....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mucor miehei; Rennin; Clotting activity; Casein; Solid-state fermentation; Submerged fermentation.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800009
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New yeast strains from the Brazilian Cerrado Biome for the production of carotenoids. Repositório Alice
FRANCO, P. F.; FALCAO, R.; CAMPANHA, R. B.; SANTOS, L. C. Z.; FAVARO, L. C. de L..
The objectives of this study were to select novel carot the major colored metabolites in the selected extracts.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Biodiversidade; Caroteno; Fermentação; Biodiversity; Beta-carotene; Sporobolomyces; Submerged fermentation.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126363
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Optimization of fermentation conditions for cellulases production by Bacillus licheniformis MVS1 and Bacillus sp. MVS3 isolated from Indian hot spring BABT
Acharya,Somen; Chaudhary,Anita.
The aim of this work was to study the effect of some nutritional and environmental factors on the production of cellulases, in particular endoglucanase (CMCase) and exoglucanases (FPase) from Bacillus licheniformis MVS1 and Bacillus sp. MVS3 isolated from an Indian hot spring. The characterization study indicated that the optimum pH and temperature value was 6.5 to 7.0 and 50-55°C, respectively. Maximum cellulases production by both the isolates was detected after 60 h incubation period using wheat and rice straw. The combination of inorganic and organic nitrogen source was suitable for cellulases production. Overall, FPase production was much higher than CMCase production by both of the strains. Between the two thermophiles, the cellulolytic activity was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Isolation; Thermophiles; Submerged fermentation; Parameter optimization; Cellulases; Hot spring.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400003
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