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Registros recuperados: 3
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Low level of pollen-mediated gene flow from cultivated to wild grapevine: consequences for the evolution of the endangered subspecies Vitis vinifera L. subsp. silvestris Inra
Di Vecchi-Staraz, M.; Laucou, V.; Bruno, G.; Lacombe, T.; Gerber, S.; Bourse, T.; Boselli, M.; This, P..
A parentage and a paternity-based approach were tested for estimation of pollen-mediated gene flow in wild grapevine (Vitis vinifera L. subsp. silvestris), a wind-pollinated species occurring in Mediterranean Europe and southwestern Asia. For this purpose, 305 seedlings collected in 2 years at 2 locations in France from 4 wild female individuals and 417 wild individuals prospected from France and Italy were analyzed using 20 highly polymorphic microsatellite loci. Their profiles were compared with a database consisting of 3203 accessions from the Institut National de la Recherche Agronomique Vassal collection including cultivars, rootstocks, interspecific hybrids, and other wild individuals. Paternity was assigned for 202 (66.2%) of the 305 seedlings,...
Tipo: Journal Article Palavras-chave: ANALYSE DE PATERNITÉ; DISPERSION DU POLLEN; PLANTE TRANSGÉNIQUE; VIGNE SAUVAGE; MICROSATELLITE; EXTRACTION D'ADN PATERNITY ANALYSIS; POLLEN DISPERSAL; ROOTSTOCK; SEEDLING; TRANSGENIC PLANT; TRUE-TO-TYPE.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20097040a34d&uri=/notices/prodinra1/2009/03/
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NMR Evidence for benzodioxane structures resulting from incorporation of 5-hydroxyconiferyl alcohol into lignins of 0-methyltransferase-deficient poplars Inra
Ralph, J.; Lapierre, C.; Lu, F.; Marita, J. M.; Pilate, G.; Van Doorsselaere, J.; Boerjan, W.; Jouanin, L..
Benzodioxane structures are produced in lignins of transgenic poplar plants deficient in COMT, an O-methyltransferase required to produce lignin syringyl units. They result from incorporation of 5-hydroxyconiferyl alcohol into the monomer supply and confirm that phenols other than the three traditional monolignols can be integrated into plant lignins.
Tipo: Journal Article Palavras-chave: LIGNINE; LIGNANE; PLANTE TRANSGENIQUE; RMN; RESONANCE MAGNETIQUE NUCLEAIRE LIGNIN; LIGNAN; TRANSGENIC PLANT; NMR.
Ano: 2001 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007fbcdf348&uri=/notices/prodinra1/2008/11/
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Transgenic rice as a novel production system for Melanocarpus and Pycnoporus laccases Inra
de Wilde, C.; Uzan, E.; Zhou, Z.; Kruus, K.; Andberg, M.; Buchert, J.; Record, E.; Asther, M.; Lomascolo, A..
Laccases have numerous biotechnological applications, among them food processing. The widespread use of laccases has increased the demand for an inexpensive and safe source of recombinant enzyme. We explored the use of a rice-based system for the production of two fungal laccases derived from the ascomycete Melanocarpus albomyces and the basidiomycete Pycnoporus cinnabarinus. High-expression levels of active recombinant laccases were achieved by targeting expression to the endosperm of rice seeds. The laccase cDNAs were fused to a plant-derived signal sequence for targeting to the secretory pathway, and placed under the control of a constitutive seed-specific promoter fused to an intron for enhanced expression. This construct enabled the recovery of on...
Tipo: Journal Article Palavras-chave: PLANTE TRANSGENIQUE; BIOTECHNOLOGIE INDUSTRIELLE; PROPRIETE BIOCHIMIQUE; ASCOMYCETE; BASIDIOMYCETE LACCASE; RECOMBINANT FUNGAL ENZYME; RICE; HETEROLOGOUS PROTEIN; TRANSGENIC PLANT; BIOCHEMICAL PROPERTIES.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008c973f6c6&uri=/notices/prodinra1/2008/10/
Registros recuperados: 3
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