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A novel chloroplastic isopentenyl diphosphate isomerase gene from Jatropha curcas: Cloning, characterization and subcellular localization Electron. J. Biotechnol.
Wei,Lei; Yin,Li; Hu,Xiaole; Xu,Ying; Chen,Fang.
Background Jatropha curcas is a rich reservoir of pharmaceutically active terpenoids. More than 25 terpenoids have been isolated from this plant, and their activities are anti-bacterial, anti-fungal, anti-cancer, insecticidal, rodenticidal, cytotoxic and molluscicidal. But not much is known about the pathway involved in the biosynthesis of terpenoids. The present investigation describes the cloning, characterization and subcellular localization of isopentenyl diphosphate isomerase (IPI) gene from J. curcas. IPI is one of the rate limiting enzymes in the biosynthesis of terpenoids, catalyzing the crucial interconversion of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Results A full-length JcIPI cDNA consisting of 1355 bp was cloned....
Tipo: Journal article Palavras-chave: Heterologous expression; Southern blot; Terpenoids; Transient expression.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600007
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Ação fumigante de terpenos sobre o Sitophilus zea mays. Repositório Alice
SANTOS, J.P.; PRATES, H.T.; WAQUIL, J.M.; OLIVEIRA, A.B..
1994
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Milho; Graos; Armazenamento; Controle; Inseticida natural; Terpenos; Sitophilus zeamais; Zea mays; Maize; Grain; Storage; Pest; Control; Insecticide; Terpenoids.
Ano: 1994 URL: http://www.alice.cnptia.embrapa.br/handle/doc/474570
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Alpha amylase and Alpha glucosidase inhibitory effects of aqueous stem extract of Salacia oblonga and its GC-MS analysis BJPS
Chelladurai,Gladis Raja Malar; Chinnachamy,Chellaram.
ABSTRACT Our present investigation deals with the phytochemical screening, estimation of total flavonoids, terpenoids and tannin contents to evaluate the anti-diabetic activities of Salacia oblonga stem followed by GC-MS analysis. It explores the natural compounds and the potential α-amylase and α-glucosidase inhibitory actions of stem extracts. The aqueous stem extract was selected from other extracts (ethanol, acetone, petroleum ether and chloroform) for the in vitro study of anti-diabetic activity by alpha amylase and alpha glucosidase inhibitory assays. The stem extract was also analyzed by gas chromatography mass spectrometry to identify the natural chemical components. Phytochemical analysis of aqueous stem extract showed major classes of secondary...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salacia oblonga; Antidiabetic; Α-Amylase; Α-Glucosidase; Terpenoids.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100606
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Anesthesia and transport of fat snook Centropomus parallelus with the essential oil of Nectandra megapotamica(Spreng.) Mez Neotropical Ichthyology
Tondolo,Juliana Simoni Moraes; Amaral,Lúcio de Paula; Simões,Larissa Novaes; Garlet,Quelen Iane; Schindler,Bianca; Oliveira,Teodoro Martins; Silva,Bruno Ferreira da; Gomes,Levy de Carvalho; Baldisserotto,Bernardo; Mallmann,Carlos Augusto; Heinzmann,Berta Maria.
This study analyzed the chemical composition and anesthetic potential of essential oil (EO) of Nectandra megapotamica in fat snook (Centropomus parallelus). For the extraction of EO by hydrodistillation, leaves were separated in young (EO-Y) or old (EO-O), and the chemical composition of the EOs was determined by CG-MS. The anesthetic potential was assessed by the evaluation of induction and recovery time of anesthesia and stress response from anesthesia and transport. Three experiments were carried out: i) four different concentrations of each EO were tested to evaluate anesthesia induction and recovery time; ii) two concentrations of EO-O were tested for the evaluation of its effects on stress parameters (glucose, lactate, and Na+ and K+ plasma levels)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Ionoregulation; Sedation; Stress; Terpenoids.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300667
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ANTIFUNGAL ACTIVITY OF NEEM (Azadirachta indica: MELIACEAE) EXTRACTS AGAINST DERMATOPHYTES Acta biol.Colomb.
OSPINA SALAZAR,Daniel Iván; HOYOS SÁNCHEZ,Rodrigo Alberto; OROZCO SÁNCHEZ,Fernando; ARANGO ARTEAGA,Myrtha; GÓMEZ LONDOÑO,Luisa Fernanda.
In order to assess the antifungal activity of methanolic extracts from neem tree (Azadirachta indica A. Juss.), several bioassays were conducted following M38-A2 broth microdilution method on 14 isolates of the dermatophytes Trichophytonmentagrophytes, Trichophyton rubrum, Microsporum canis and Epidermophyton floccosum. Neem extracts were obtained through methanol-hexane partitioning of mature green leaves and seed oil. Furthermore, high performance liquid chromatography (HPLC) analyses were carried out to relate the chemical profile with their content of terpenoids, ofwidely known antifungal activity. The antimycotic Terbinafine served as a positive control. Results showed that there was total growth inhibition of the dermatophytes isolates at minimal...
Tipo: Journal article Palavras-chave: HPLC; Neem; Microbial sensitivity tests; Minimum inhibitory concentration; Terpenoids.
Ano: 2015 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000300017
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Attractiveness of host plant volatile extracts to the asian citrus psyllid, Diaphorina citri, is reduced by terpenoids from the non-host cashew. Repositório Alice
FANCELLI, M.; BORGES, M.; LAUMANN, R. A.; PICKETT, J. A.; BIRKETT, M. A.; BLASSIOLI-MORAES, M. C..
bitstream/item/176806/1/10.1007-s10886-018-0937-1.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Asian citrus psyllid; Plant insect interactions; Non-host; DMNT; TMTT; Citrus; Hosts; Cashew fruit; Terpenoids.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1091431
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Attractiveness of host plant volatile extracts to the asian citrus psyllid, Diaphorina citri, is reduced by terpenoids from the non-host cashew. Repositório Alice
FANCELLI, M.; BORGES, M.; LAUMANN, R. A.; PICKETT, J. A.; BIRKETT, M. A.; BLASSIOLI-MORAES, M. C..
Diaphorina citri is a vector of the bacterial causative agent of Huanglongbing (HLB?=?Citrus greening), a severe disease affecting citrus crops. As there is no known control for HLB, manipulating insect behaviour through deployment of semiochemicals offers a promising opportunity for protecting citrus crops. The behavioural responses of D. citri to plant volatiles, and the identity of these plant volatiles were investigated. Volatiles were collected from host plants Murraya paniculata, Citrus sinensis, C. reshni, C. limettioides, Poncirus trifoliata, and from non-host plants Psidium guajava, Mangifera indica, Anacardium occidentale. In behavioural assays, female D. citri spent more time in the arms containing volatiles from either M. paniculata or C....
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Asian citrus psyllid; Plant insect interactions; Non-host; DMNT; TMTT; Citrus; Hosts; Cashew fruit; Terpenoids.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1096420
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Chemical, Antioxidant, and Antimicrobial Evaluation of Essential Oils and an Anatomical Study of the Aerial Parts from Baccharis Species (Asteraceae) Repositório Alice
ZUCCOLOTTO, T.; BRESSAN, J.; LOURENÇO, A. V. F.; BRUGINSKI, E.; VEIGA, A.; MARINHO, J. V. N.; RAESKI, P. A.; HEIDEN, G.; SALVADOR, M. J.; MURAKAMI, F. S.; BUDEL, J. M.; CAMPOS, F. R..
bitstream/item/205789/1/Gustavo-Heiden-Zuccolotto-et-al-2019-Chemistry-Biodiversity.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Anatomical study; Antimicrobial activity; Baccharis; Essential oils; Terpenoids; Antioxidant activity; Biological activity in soil.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115459
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Chemical composition and antibacterial activity of Brazilian propolis essential oil J. Venom. Anim. Toxins incl. Trop. Dis.
Oliveira,AP; França,HS; Kuster,RM; Teixeira,LA; Rocha,LM.
The present study aimed at investigating the chemical composition of essential oil extracted from Brazilian propolis and the susceptibility of Staphylococcus aureus, Staphylococcus epidermides, Streptococcus pyogenes and Escherichia coli to this substance. The essential oil was obtained by steam distillation of propolis and examined by gas chromatography/mass spectrometry (GC/MS). In addition, the agar diffusion method using filter paper disks was employed. Antibacterial activity was measured as equivalent diameters of inhibition zones (in millimeters) after incubation at 37º C for 24 hours. From the 26 identified constituents, β-caryophyllene (12.7%), acetophenone (12.3%) and β-farnesene (9.2%) were found to be major components. New components, namely...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apis mellifera; Propolis essential oil; Terpenoids; Antibacterial activity.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100012
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Chemical constituents of Piptadenia gonoacantha (Mart.) J.F. Macbr (pau jacaré) Anais da ABC (AABC)
Carvalho,Mário G. de; Cardozo,Maritza A.R.; Catunda Junior,Francisco E.A.; Carvalho,Acácio G. de.
The phytochemical investigation of Piptadenia gonoacantha (Mart.) J.F. Macbr. (Leguminosae-Mimosoideae), commonly known as "pau jacaré" (alligator stick), afforded sitosterol, campesterol, stigmasterol, the N-benzoylphenylalanine-2-benzoylamide-3-phenylpropyl ester, known as asperphenamate, sitosterol-3-O-β-D-glucopyranoside, besides three flavonoids, apigenin, 5-O-methylapigenin and 7,4'-dihydroxy-3',5-dimethoxyflavone from its branches. From its leaves, the methyl gallate and two flavonoids, vitexin and isovitexin, were isolated. From its bark, a mixture of sitosterol, campesterol, and stigmasterol, besides a mixture of cycloartenone, cycloartan-25-en-3-one, and 24-methylene-cycloartenone, and the pure triterpenes 24-methylenecycloartanol, friedelin,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leguminoseae; Piptadenia gonoacantha; Terpenoids; Asperphenamate; Flavonoids; "pau jacaré"; Piptadenia gonoacantha.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000300003
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Eficácia sinérgica de diferentes frações do óleo essencial de Piper aduncum L. para inseticidas utilizados no controle da lagarta-do-cartucho-do-milho. Repositório Alice
LIMA, R. P. Q de; MONTEIRO, A. F. M.; FAZOLIN, M..
Sinergistas como o óleo essencial de Piper aduncum L. (OEPA) são utilizados no manejo da resistência. O OEPA apresenta o dilapiol como componente majoritário, e estes compostos geralmente determinam as propriedades biológicas, sendo possível a modulação adicional de outros compostos minoritários. O objetivo desse trabalho foi o de avaliar a eficácia sinérgica de 6 frações do OEPA, para os inseticidas deltametrina e bifentrina.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Dilapiol; Dilapidol; Bifentrina; Deltametrina; Terpeno; Laboratório de Entomologia; Embrapa Acre; Rio Branco (AC); Acre; Amazônia Ocidental; Western Amazon; Amazonia Occidental; Pimenta de macaco; Óleo essencial; Sinergismo; Inseticida piretróide; Piper aduncum; Essential oils; Terpenoids; Synergism; Pyrethroid insecticides; Deltamethrin; Bifenthrin; Aceites esenciales; Terpenoidos; Insecticidas con piretroides; Deltametrín; Bifentrin.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1089845
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Foliar micromorphology and anatomy of Ugni molinae Turcz. (Myrtaceae), with particular reference to schizogenous secretory cavities RChHN
Retamales,Hernán A; Scherson,Rosa; Scharaschkin,Tanya.
BACKGROUND: Ugni molinae Turcz. is one of the most studied species of South American Myrtaceae due to its edible fruits and foliar medicinal compounds. However, there is no anatomical study of the leaves or secretory cavities. This paper seeks to describe the leaf micromorphology and anatomy of the species using standard protocols for light and scanning electron microscopy. Secretory cavities were anatomically characterized in young and mature leaves. Histochemical staining of the cavities was performed. RESULTS: The leaves of U. molinae are hypostomatic, have a wavy surface and possess scattered hairs. Leaf anatomical features include dorsiventral mesophyll, two to three layers of palisade parenchyma with abundant chloroplasts, calcium oxalate crystals...
Tipo: Journal article Palavras-chave: Anatomy; Myrtaceae; Secretory cavities; SEM; Terpenoids.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2014000100024
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Growth regulators and essential oil production Braz. J. Plant Physiol.
Prins,Cláudia L; Vieira,Ivo J. C; Freitas,Silvério P.
The aroma and fragrance industry is a billion-dollar world market which grows annually. Essential oils comprise the majority of compounds used by these industries. These sets of metabolites are formed mainly by monoterpenes, which are products of the plants' secondary metabolism. Biosynthesized from mevalonate and methylerythitol phosphate, the essential oil production depends not only on genetic factors and the developmental stage of plants, but also on environmental factors which could result in biochemical and physiological alterations in plants modifying the quantity and quality of the essential oil. These modifications impair aromatic plant production aimed at essential oil by reducing their quality. It is desirable to develop techniques of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Auxins; Cytokinins; Gibberellins; Ethylene; Jasmonate; Terpenoids.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000200003
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Monoterpenoids (thymol, carvacrol and S-(+)-linalool) with anesthetic activity in silver catfish (Rhamdia quelen): evaluation of acetylcholinesterase and GABAergic activity BJMBR
Bianchini,A.E.; Garlet,Q.I.; da Cunha,J.A.; Bandeira Junior,G.; Brusque,I.C.M.; Salbego,J.; Heinzmann,B.M.; Baldisserotto,B..
This study evaluated the anesthetic potential of thymol and carvacrol, and their influence on acetylcholinesterase (AChE) activity in the muscle and brain of silver catfish (Rhamdia quelen). The AChE activity of S-(+)-linalool was also evaluated. We subsequently assessed the effects of thymol and S-(+)-linalool on the GABAergic system. Fish were exposed to thymol and carvacrol (25, 50, 75, and 100 mg/L) to evaluate time for anesthesia and recovery. Both compounds induced sedation at 25 mg/L and anesthesia with 50–100 mg/L. However, fish exposed to carvacrol presented strong muscle contractions and mortality. AChE activity was increased in the brain of fish at 50 mg/L carvacrol and 100 mg/L thymol, and decreased in the muscle at 100 mg/L carvacrol....
Tipo: Info:eu-repo/semantics/article Palavras-chave: AChE; Anesthesia; Benzodiazepine; Fish; GABAA; Terpenoids.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001200610
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Secondary metabolites from marine microorganisms Anais da ABC (AABC)
KELECOM,ALPHONSE.
After 40 years of intensive research, chemistry of marine natural products has become a mature field. Since 1995, there are signals of decreased interest in the search of new metabolites from traditional sources such as macroalgae and octocorals, and the number of annual reports on marine sponges stabilized. On the contrary, metabolites from microorganisms is a rapidly growing field, due, at least in part, to the suspicion that a number of metabolites obtained from algae and invertebrates may be produced by associated microorganisms. Studies are concerned with bacteria and fungi, isolated from seawater, sediments, algae, fish and mainly from marine invertebrates such as sponges, mollusks, tunicates, coelenterates and crustaceans. Although it is still to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteria; Fungi; Alkaloids; Despsipeptides; Terpenoids; Acetogenins; Biological activities.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000100012
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Tempo de evaporação de terpenos visando testes de fumigação. Repositório Alice
PRATES, H.T.; OLIVEIRA, A.B.; SANTOS, J.P.; WAQUIL, J.M..
1994
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Milho; Praga; Inseticida natural; Produtos naturais; Terpenos; Zea mays; Maize; Insecticide; Fumigation; Terpenoids.
Ano: 1993 URL: http://www.alice.cnptia.embrapa.br/handle/doc/472594
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Terpene production in the peel of sweet orange fruits Genet. Mol. Biol.
Takita,Marco A.; Berger,Irving J.; Basílio-Palmieri,Ana Carolina; Borges,Kleber M.; Souza,Juliana M. de; Targon,Maria L.N.P..
Terpenoids constitute the largest and most diverse class of natural products. They are important factors for aroma and flavor, and their synthesis is basically done from two compounds: isopentenyl diphosphate and dimethylallyl diphosphate. Isopentenyl diphosphate is synthesized through two different pathways, one that occurs in the cytoplasm and one in the plastid. With the sequencing of ESTs from citrus, we were able to perform in silico analyses on the pathways that lead to the synthesis of terpenes as well as on the terpene synthases present in sweet orange. Moreover, expression analysis using real-time qPCR was performed to verify the expression pattern of a terpene synthase in plants. The results show that all the pathways for isopentenyl diphosphate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: EST; Fruit; Terpenoids; Orange; Essential oil.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500012
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