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| Shundo,Luzia; Sabino,Myrna. |
| During 2002 and 2003, a total of 107 samples of raw, pasteurized and ultrahigh treated temperature (UHT) milk commercialized in the cities of São Paulo and Marília (SP) were analyzed for the presence of aflatoxin M1 (AFM1). AFM1 was detected in 79 (73.8%) of milk samples, ranging from <0.02 to 0.26 mug/L.The samples were analyzed using an immunoaffinity column for cleanup and a thin layer chromatography for determining AFM1. The parameters, such as recovery, repeatibility, detection and quantification limit were evaluated to optimize this method (in-house). Based on spiked samples, the recovery values ranged from 85.83 to 73.86% at levels of 0.010-0.50 mug/L, respectively, and the relative standard deviation for repeatibility ranged from 7.73 to 2.08%.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Milk; Aflatoxin M1; Thin layer chromatography; Immunoaffinity column. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200013 |
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| Sawaya,Alexandra C. H. F.; Souza,Kirllian S.; Marcucci,Maria C.; Cunha,Ildenize B.S.; Shimizu,Mario T.. |
| Brazilian propolis from São Paulo state was submitted to extraction using several solvents, resulting in extracts with different composition. These extracts were submitted to Thin Layer Chromatography (TLC). Bioauthographic analysis of the TLC plates identified fractions with inhibitory activity, which were then analysed by High Performance Liquid Chromatography (HPLC). In vitro assays, commonly used to evaluate the activity of propolis against Gram-positive bacteria, were compared to determine which rendered the most consistent results. The bactericidal activity of these extracts were analysed by Serial Dilution in Tubes and Agar Plate Diffusion. Serial Dilution in Tubes obtained the most consistent results, with the Minimal Bactericidal Concentration of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Propolis; Thin layer chromatography; Bioauthography; High performance liquid chromatography; Extraction methods; Minimal bactericidal concentration. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100017 |
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| GUERRERO,CARLA; ESPINOZA,LUIS; NIEMEYER,HERMANN M; SIMONETTI,JAVIER A. |
| The distribution and habitat use by carnivores can be assessed by studying their tracks or feces. If these methods are to be used confidently, they should not only unequivocally discriminate among species, but should also render the same patterns of spatial distributions. We assessed the fulfillment of these requirements with five carnivores inhabiting the Maulino forest of central Chile: Galictis cuja, Oncifelis guigna, Pseudalopex culpaeus, Pseudalopex griseus, and Puma concolor. Fecal bile acid thin layer chromatographic profiles were assessed, and shown to be species-specific, invariant within samples of a given individual and among individuals of a given species, but consistently different across species. The spatial distribution of feces in mixed... |
| Tipo: Journal article |
Palavras-chave: Fecal bile acids; Galictis; Habitat use; Oncifelis; Pseudalopex; Puma; Thin layer chromatography. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2006000100008 |
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