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| LUSTRINO,DANILO; SILVA,ALBA C.M.; ARAUJO,IRACEMA G.; TUNHOLI,VICTOR M.; TUNHOLI-ALVES,VINÍCIUS M.; CASTRO,ROSANE N.; CARVALHO,DENISE P.; PINHEIRO,JAIRO; MARASSI,MICHELLE P.. |
| ABSTRACT The objective of this study was to identify thyroid hormones and to examine their putative site of synthesis in Achatina fulica snails. For this purpose, radioimmunoassays were performed for T3 and T4 before and after long starvation with or without hemolymph deproteinization. Sodium/iodide symporter activity in vivo was analyzed through 125I administration with and without KClO4 pretreatment. Only T4 was detected, and its concentration decreased due to starvation or deproteinization. However, high-performance liquid chromatography analysis also showed the presence of T2 and T3 apart from T4, but rT3 was not detected in the A. fulica hemolymph. The sodium/iodide symporter activity was greater in cerebral ganglia than digestive gland, but KClO4... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Achatina fulica; Sodium/iodine symport; Thyroid hormones; Thyroglobulin. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502181 |
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| Boechat,L.H.B.; Zollner,R.L.. |
| Studies concerning the antigenicity of thyroglobulin fragments allow the characterization of the epitopes but do not consider the role of heavier antigenic fragments that could result in vivo from the action of endoproteases. Here we assess the relative importance of the fragments obtained from thyroglobulin by limited proteolysis with trypsin and compare by immunoblotting their reactivity to serum from patients with autoimmune (Graves' disease and Hashimoto's thyroiditis) and non-autoimmune (subacute thyroiditis) disease. The results showed no difference in frequency of recognition of any peptide by sera from patients with autoimmune thyroiditis. In contrast, sera from patients with subacute thyroiditis reacted more frequently with a peptide of 80 kDa.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Autoantibodies; Autoimmunity; Thyroglobulin; Trypsin; Thyroiditis; Proteases. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400012 |
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