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| Freire,Laurineide Lopes de Carvalho; Costa,Ana Bolena Lima da; Góes,Larissa Brandão; Oliveira,Neiva Tinti de. |
| Five mutants (MaE10, MaE27, MaE24, MaE41 e MaE49) of Metarhizium anisopliae wild strain E9 were analysed for DNA profile through the RAPD technique and for changes in total protein content by spectrophotometry, polyacrylamide gel electrophoresis and densitometry. The pattern of RAPD markers showed genetic polymorphism among the strains: out of twenty primers seven were selected, producing 113 bands. Forty seven bands were present in all strains (41.6% of monomorphic bands) and 66 showed polymorphism (58.4%). The mean coefficient of similarity among all strains was 0.75 (75%). The total protein content varied, staining in the interval of 6.0-8.0 µg/µl. The electrophoresis analysis, through zymogram and protein fraction profiles by densitometry, allowed the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metarhizium anisopliae; Mutants; RAPD; Total protein. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200004 |
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| Ribeiro,A.P.; Escobar,A.; Motheo,T.F.; Sakamoto,C.A.M.; Godoy,G.S.; Costa,M.A.N.; Laus,J.L.. |
| Efficacy of carprofen, administered by different routes, was studied in experimental uveitis in dogs. Anterior chamber paracenteses was accomplished at two different moments (M0 and M1), with a five hour interval between them. At M0 and M1, 0.2mL of aqueous humor was collected and quantitation of total protein and prostaglandin E2 (PGE2) were determined. Four groups were formed (n=8), which received carprofen at the end of M0, by the following routes: subcutaneous (GIm), subconjunctival (GII), and topical (GIII). A fourth group that received no treatment was instituted (Control). Conjunctival histopathology of the GII animals was performed. In all groups, values of protein and PGE2 significantly enhanced at M1; however, they did not significantly change... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Uveitis; Carprofen; Total protein; Prostaglandin E2. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000200010 |
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| Ribeiro,Alexandre Pinto; Escobar,André; Motheo,Tathiana Fergunson; Godoy,Guilherme Selera; Laus,José Luiz. |
| Efficacy of meloxicam, administered by different routes was studied in experimental uveitis in dogs. Anterior chamber paracenteses was accomplished at two different moments (M0 and M1), with a five hour interval among them. At M0 and M1, 0.2mL of aqueous humor was collected and total protein and prostaglandin E2 (PGE2) quantitation was determined. Four groups were formed (n=5), which received meloxicam at the end of M0. by the following routes: subcutaneous (GIm), subconjunctival (GII), and topical (GIII). A fourth group that received no treatment was instituted (Control). Conjunctival histopathology of the GII was performed. Results were evaluated statistically (P≤0.05). In all groups, protein and PGE2 values enhanced significantly in M1. Protein and PGE2... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Uveitis; Meloxicam; Total protein; Prostaglandin E2; Dogs. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000700024 |
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