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| Xu,Xiaochun; Wei,Xuan; Yang,Yuxin; Niu,Wenzhi; Kou,Qifang; Wang,Xiaolong; Chen,Yulin. |
| Background The objective of this study was to investigate proliferator-activated receptor (PPARγ), fatty acid synthase (FAS) and hormone-sensitive lipase (HSL) mRNA and protein expression in fat tails of Tan sheep. Rams from different developmental stages (aged 3, 6, 9, 12, 15 and 18 months) were selected, and their tail measurements including length (L), width (W) and girth (G) were recorded. The mRNA and protein expressions of PPARγ, FAS and HSL were examined by quantitative real-time polymerase chain reaction (PCR) and Western blot. Results The tail measurements increased with age. We observed no significant differences (P > 0.05) of PPARγ mRNA expression between ages 9 and 15 months, and between 12 and 15 months; FAS... |
| Tipo: Journal article |
Palavras-chave: Fat-tailed sheep; MRNA transcription; Protein expression; Transcription factor and enzyme genes. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200010 |
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