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	Provedor de dados Repositório Alice (9) Genet. Mol. Biol. (3) Open Agri (2) Biol. Res. (2) ArchiMer (2) BABT (2) BJMBR (2) Colegio de Postgraduados (1) Electron. J. Biotechnol. (1) Phyton (1) Mais... Autor NEPOMUCENO, A. L. (4) NAKASHIMA, K. (3) FARIAS, J. R. B. (2) FUGANTI-PAGLIARINI, R. (2) GROSSI-DE-SA, M. F. (2) GRYNBERG, P. (2) MARIN, S. R. R. (2) MERTZ-HENNING, L. M. (2) MOLINARI, H. B. C. (2) NEUMAIER, N. (2) RODRIGUES, F. A. (2) TOGAWA, R. C. (2) YAMAGUCHI-SHINOZAKI, K. (2) AFONSO, J. (1) ALVES-FERREIRA, M. (1) AMORIM, R. M. S. (1) ANTONINO, J. D. (1) ARRAES, F. B. M. (1) Angenent,Gerco C. (1) BARBOSA, D. de A. (1) Mais... Palavra-chave Transcription factors (25) Abiotic stress (4) Soja (3) Development (2) Genes (2) Glycine Max (2) Soybeans (2) Transcription (2) 9-cisepoxycarotenoid dioxygenase (1) ABA (1) AP2/ERF (1) AREB (1) Acute respiratory distress syndrome (1) Algae (1) Androgens (1) Animal breeding (1) Apoptosis (1) Arabidopsis (1) Arnt (1) Ascidian (1) Mais... Tipo do documento Info:eu-repo/semantics/article (8) Artigo de periódico (3) Journal article (3) Separatas (3) Parte de livro (2) Text (2) Anais e Proceedings de eventos (1) Mais... Ano 2022 (1) 2021 (1) 2020 (3) 2019 (1) 2018 (3) 2016 (5) 2015 (1) 2013 (1) 2012 (1) 2010 (2) 2009 (1) 2007 (2) 2006 (1) 2005 (1) 2004 (1) Mais... País Brazil (16) Chile (3) France (2) India (2) Argentina (1) Mexico (1) Mais... Idioma Inglês (20) Espanhol (1) Italiano (1) Português (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 25 Primeira ... 1 2 ... Última A phage display combined with DNA affinity magnetic system can be applied to a screening of DNA binding proteins, such as transcription factors Electron. J. Biotechnol. Yulita,Kusumadewi Sri; Kouno,Takafumi; Ezaki,Bunichi. Here we introduce a new approach for the screening of DNA binding proteins, using a phage library based on a phage display technique. In principal, a complementary DNA (cDNA) library based on the recombinant bacteriophage T7 expressing target proteins on its capsid (phage display) is constructed. These phage particles are hybridized with a biotinylated target DNA fragment which is immobilized on the surface of streptavidin paramagnetic particle (SA-PMP). The phage particles are released from the target DNA fragment by a nuclease treatment and the recovered phages are used to the next round of hybridization. These processes are repeated three times to amplify the target phages in the population. This simple method is faster, and more systemic than other... Tipo: Journal article Palavras-chave: AtGST11 gene; Biopanning; DNA binding proteins; T7 phage differential display; Transcription factors. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100014 Agronomic evaluation of genetically modified soybean genotypes in response to water deficit. Repositório Alice LIMA, L. F. S. de; FERREIRA, L. C.; FUGANTI-PAGLIARINI, R.; MARIN, S. R. R.; OLIVEIRA, M. C. N. de; MERTZ-HENNING, L. M.; FARIAS, J. R. B.; NEUMAIER, N.; NAKASHIMA, K.; NUNES, L. M.; NEPOMUCENO, A. L.. Título em português: Avaliações agronômicas de soja geneticamente modificada em resposta ao déficit hídrico. Tipo: Separatas Palavras-chave: Resistência a Seca; Soja; Fator de Crescimento; Soybeans; Drought tolerance; Transcription factors; Plant growth. Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102092 An effective method for cloning of partial MADS-box genes related to flower development in groundnut Open Agri Yuan, M.. Palavras-chave: Genes; PCR; DNA; Cloning; Transcription factors; Genomes; Transcription; Genetic structures; Nucleotides; Molecular cloning. Ano: 2005 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3213 Analysis of the SHP2 enhancer for the use of tissue specific activation tagging in Arabidopsis thaliana Genet. Mol. Biol. Chalfun-Junior,Antonio; Mes,Jurriaan J.; Busscher,Marco; Angenent,Gerco C.. Activation tagging is a powerful tool to identify new mutants and to obtain information about possible biological functions of the overexpressed genes. The quadruple cauliflower mosaic virus (CaMV) 35S enhancer fragment is a strong enhancer, which is most commonly used for this purpose. However, the constitutive nature of this enhancer may generate lethal mutations or aberrations in different plant organs by the same overexpressed gene. A tissue-specific activation tagging approach may overcome these drawbacks and may also lead more efficiently to the desired phenotype. For this reason the SHATTERPROOF2 (SHP2) promoter fragment was analysed for enhancer activity. The SHP2 gene is involved in dehiscence zone development and expressed during silique... Tipo: Info:eu-repo/semantics/article Palavras-chave: Arabidopsis; Development; Pod shattering; Transcription factors; Mutants. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200032 Analyzing gene expression profiles in dilated cardiomyopathy via bioinformatics methods BJMBR Wang,Liming; Zhu,L.; Luan,R.; Wang,L.; Fu,J.; Wang,X.; Sui,L.. Dilated cardiomyopathy (DCM) is characterized by ventricular dilatation, and it is a common cause of heart failure and cardiac transplantation. This study aimed to explore potential DCM-related genes and their underlying regulatory mechanism using methods of bioinformatics. The gene expression profiles of GSE3586 were downloaded from Gene Expression Omnibus database, including 15 normal samples and 13 DCM samples. The differentially expressed genes (DEGs) were identified between normal and DCM samples using Limma package in R language. Pathway enrichment analysis of DEGs was then performed. Meanwhile, the potential transcription factors (TFs) and microRNAs (miRNAs) of these DEGs were predicted based on their binding sequences. In addition, DEGs were mapped... Tipo: Info:eu-repo/semantics/article Palavras-chave: Dilated cardiomyopathy; Differentially expressed genes; Pathway enrichment analysis; Transcription factors; MicroRNAs; Small molecules. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000706 Apoptosis induction by E2F decoy DNA of the prostate cancer cell line BABT Wang,Tao; Jiang,An -li; Zhang,Jian-ye; Jing,Fei. To observe effect of E2F decoy DNA on proliferation and apoptosis of androgen-independent prostate cancer cell line PC-3M the binding specificity of the E2F decoy DNA to the PC-3M nuclear protein was detected by electrophoretic mobility shift assay (EMSA). E2F decoy DNA, ARE decoy DNA and Control decoy DNA were respectively transfected into PC-3M cells with lipofectamine.Their influence on cell proliferative activity was detected by MTT assay.The cell apoptotic rate was determined by flow cytometric(FCM) analysis and chromosome DNA ladder was detected by DNA gel electrophoresis.The change of mRNA expression of C-myc and CyclinD1 were detected by RT-PCR.The change of mRNA expression of C-myc and CyclinD1 were detected by Western-blot. EMSA demonstrated... Tipo: Info:eu-repo/semantics/article Palavras-chave: Decoy DNA; Transcription factors; PC-3M cells; Apoptosis. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200011 Candidate genes and pathogenesis investigation for sepsis-related acute respiratory distress syndrome based on gene expression profile Biol. Res. Wang,Min; Yan,Jingjun; He,Xingxing; Zhong,Qiang; Zhan,Chengye; Li,Shusheng. BACKGROUND: Acute respiratory distress syndrome (ARDS) is a potentially devastating form of acute inflammatory lung injury as well as a major cause of acute respiratory failure. Although researchers have made significant progresses in elucidating the pathophysiology of this complex syndrome over the years, the absence of a universal detail disease mechanism up until now has led to a series of practical problems for a definitive treatment. This study aimed to predict some genes or pathways associated with sepsis-related ARDS based on a public microarray dataset and to further explore the molecular mechanism of ARDS RESULTS: A total of 122 up-regulated DEGs and 91 down-regulated differentially expressed genes (DEGs) were obtained. The up- and down-regulated... Tipo: Journal article Palavras-chave: Acute respiratory distress syndrome; Sepsis; Differentially expressed mRNAs; Functional enrichment analysis; Pathway analysis; Transcription factors. Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100025 Cloning and Overexpression of GmDREB2 Gene from a Vietnamese Drought-resistant Soybean Variety BABT Tan,Dao Xuan; Tuong,Ho Manh; Thuy,Vu Thi Thu; Son,Le Van; Mau,Chu Hoang. ABSTRACTThis work studied the amplification, cloning and determination of the GmDREB2 gene from the soybean cultivar DT2008 and five Vietnamese local soybean cultivars (DT26, DT51, DVN5, CB, CBD) and designed the vector carrying the structure containing GmDREB2 gene from cultivar DT2008 (best drought tolerant). The coding region of GmDREB2 gene isolated from six soybean cultivars was 480 nucleotides in length, encoding 159 amino acids. The recombinant structure was designed as 35S- GmDREB2- c-myc and its expression was analysed in transgenic tobacco plants. Recombinant DREB2 protein was expressed in five transgenic tobacco lines with molecular weights close to 20 kDa. During the drought conditions, the proline accumulation of the transgenic tobacco lines... Tipo: Info:eu-repo/semantics/article Palavras-chave: Drought-resistant; GmDREB2 gene; Gene transfer; Soybean; Transcription factors. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500651 Comparative gut transcriptome analysis of Diatraea saccharalis in response to the dietary source. Repositório Alice NORIEGA, D. D.; ARRAES, F. B. M.; ANTONINO, J. D.; MACEDO, L. L. P.; FONSECA, F. C. A.; TOGAWA, R. C.; GRYNBERG, P.; SILVA, M. C. M.; NEGRISOLI JUNIOR, A. S.; MORGANTE, C. V.; GROSSI-DE-SA, M. F.. Tipo: Artigo de periódico Palavras-chave: Physiological Aspects; Genetic Aspects; Diatraea Saccharalis; Transcription factors. Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1124729 Development of drought-tolerant sugarcane overexpressing the AtDREB2A CA gene. Repositório Alice SOUZA, W. R. de; OLIVEIRA, N. G. de; VINECKY, F.; RIBEIRO, A. P.; BASSO, M. F.; CASARI, R. A. das C. N.; DIAS, B. B. A.; NEPOMUCENO, A. L.; KOBAYASHI, A. K.; NAKASHIMA, K.; YAMAGUCHI-SHINOZAKI, K.; MOLINARI, H. B. C.. Sugarcane is considered an important economic crop not only for sugar production, but also for ethanol generation, serving as an expandable green alternative to the usage of crude oil. To take advantage of the use of sugarcane as a renewable source for bioethanol production, it is important to increase its productivity without increasing land usage, which includes sugarcane cultivation under hostile conditions, such as waterlimited environments. In this study, we demonstrated that stress-inducible overexpression of the transcription factor AtDREB2A CA conferred drought tolerance in sugarcane subjected to water deficiency under greenhouse conditions. In addition, transgenic plants expressing AtDREB2A CA accumulated higher levels of sucrose than... Tipo: Parte de livro Palavras-chave: AtDREB2A CA; ZmRab17 promoter; Abiotic stress; Saccharum; Transcription factors. Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1130044 Differential impact of acclimation and acute water deprivation in the expression of key transcription factors in soybean roots. Repositório Alice GUIMARÃES-DIAS, F.; NEVES-BORGES, A. C.; CONFORTE, A. J.; GIOVANELLA-KAMPMANN, L.; FERREIRA, A. V. J.; AMORIM, R. M. S.; BENEVENT, M. A.; SÁ, M. E. L. de; MESQUITA, R. O.; RODRIGUES, F. A.; NEPOMUCENO, A. L.; ROMANO, E.; LOUREIRO, M. E.; GROSSI-DE-SA, M. F.; ALVES-FERREIRA, M.. Tipo: Separatas Palavras-chave: Drought adaptation; Marker assistance selection; Water deficit; Glycine Max; Transcription factors. Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1056209 DREB1A allows for more water uptake in groundnut by a large modification in the root/shoot ratio under water deficit Open Agri Vadez, V.. Palavras-chave: Transgenics; Evapotranspiration; Drought stress; Genotypes; Nutrient uptake; Transgenic plants; Transpiration (animals); Harvest index; Transcription; Transcription factors. Ano: 2007 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3355 Drought-tolerant soybean development: evaluation of GM lines under greenhouse and field conditions. Repositório Alice FUGANTI-PAGLIARINI, R.; MARIN, S. R. R.; MOLINARI, M. D. C.; BARBOSA, D. de A.; MOLINARI, H. B. C.; MERTZ-HENNING, L. M.; NEUMAIER, N.; FARIAS, J. R. B.; NAKASHIMA, K.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L.. Tipo: Parte de livro Palavras-chave: DREB; AREB; ABA; Galactinol synthase; 9-cisepoxycarotenoid dioxygenase; Soja; Glycine Max; Deficiência Hídrica; Produção; Transcription factors; Soil water deficit; Yields. Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1128419 Identification, Structure Analyses and Expression Pattern of the ERF Transcription Factor Family in Coffea arabica. Repositório Alice SOUZA, S. G. H. de; SANTOS, T. B. dos; DOMINGUES, D. S.; BERNADAC, A.; BOUZAYEN, M.; PEREIRA, L. F. P.; DEGRASSI, G.; CARPENTIERI-PIPOLO, V.. Members of the ERF Family of Transcription Factors play an important role in plant development and gene expression that regulates responses to biotic and abiotic stress. This work identified 36 ERF family genes in Coffea arabica within the AP2/ERF full domain, using the EST-based genomic resource of the Brazilian Coffee Genome Project. The ERF family genes were classified into nine of the ten existing groups through phylogenetic analysis of the deduced amino acid sequences and comparison with the sequences of the ERF family genes in Arabidopsis. In addition to the AP2 domain, other conserved domains were identified, typical of members of each group. The in silico analysis and expression profiling showed high levels of expression for libraries derived from... Tipo: Artigo de periódico Palavras-chave: AP2/ERF; Coffee; Ethylene; Transcription factors. Ano: 2021 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137126 Msxb is a core component of the genetic circuitry specifying the dorsal and ventral neurogenic midlines in the ascidian embryo ArchiMer Roure, Agnes; Darras, Sebastien. The tail ascidian larval peripheral nervous system is made up of epidermal sensory neurons distributed more or less regularly in ventral and dorsal midlines. Their formation occurs in two-steps: the ventral and dorsal midlines are induced as neurogenic territories by Fgf9/-16/20 and Admp respectively. The Delta2/Notch interaction,then controls the number of neurons that form. The genetic machinery acting between the inductive processes taking place before gastrulation and neuron specification at tailbud stages are largely unknown. The analysis of seven transcription factors expressed in the forming midlines revealed an unexpected complexity and dynamic of gene expression. Their systematic overexpression confirmed that these genes do not interact following... Tipo: Text Palavras-chave: Ascidian; Gene regulatory network; Transcription factors; Peripheral nervous system; Neurogenic. Ano: 2016 URL: https://archimer.ifremer.fr/doc/00374/48557/48984.pdf Overall picture of expressed Heat Shock Factors in Glycine max, Lotus japonicus and Medicago truncatula. Repositório Alice SOARES-CAVALCANTI, N. M.; BELARMINO, L. C.; KIDO, E. A.; PANDOLFI, V.; MARCELINO-GUIMARÃES, F. C.; RODRIGUES, F. A.; PEREIRA, G. A. G.; BENKO-ISEPPON, A. M.. Heat shock (HS) leads to the activation of molecular mechanisms, known as HS-response, that prevent damage and enhance survival under stress. Plants have a flexible and specialized network of Heat Shock Factors (HSFs), which are transcription factors that induce the expression of heat shock proteins. The present work aimed to identify and characterize the Glycine max HSF repertory in the Soybean Genome Project (GENOSOJA platform), comparing them with other legumes (Medicago truncatula and Lotus japonicus) in view of current knowledge of Arabidopsis thaliana. The HSF characterization in leguminous plants led to the identification of 25, 19 and 21 candidate ESTs in soybean, Lotus and Medicago, respectively. A search in the SuperSAGE libraries revealed 68... Tipo: Artigo de periódico Palavras-chave: Bioinformática; Soja; Gene; Genoma; Bioinformatics; Soybeans; Genome; Genes; Abiotic stress; Transcription factors. Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/926601 Possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cells Biol. Res. JAIMOVICH,ENRIQUE; ESPINOSA,ALEJANDRA. We studied the effect of IGF-I, insulin and testosterone on intracellular Ca2+ in cultured muscle cells. Insulin produced a fast (<1 s) and transient [Ca2+] increase lasting less than 10 s. IGF-I induced a transient [Ca2+] increase, reaching a fluorescence peak 6 s after stimulus, to return to basal values after 60 s. Testosterone induced delayed (35 s) and long lasting (100-200 s) signals, frequently associated with oscillations. IGF-I, testosterone and electrical stimulation-induced Ca2+ signals were shown to be dependent on IP3 production. All of these Ca2+ signals were blocked by inhibitors of the IP3 pathway. On the other hand, insulin-induced Ca2+ increase was dependent on ryanodine receptors and blocked by either nifedipine or ryanodine. The... Tipo: Journal article Palavras-chave: Skeletal; Transcription factors; Insulin; IGF-I; Testosterone. Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400018 Prediction and identification of miRNAS in Elaeis Guinensis Jacq and analysis of their expression in oil palm plants under salinity drought stress. Repositório Alice SALGADO, F. F.; VIEIRA, L. R.; SILVA, V, N. B.; LEAO, A. P.; GRYNBERG, P.; COSTA, M. M. do C.; TOGAWA, R. C.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T.. Oil palm (Elaeis guineensis Jacq.) is an oilseed crop of great economic importance. The oil palm industry has a large-scale production worldwide and uses high efficient extraction and refining processes to obtain palm oil and palm kernel oil. In Brazil, currently, there is an extensive area suitable for oil palm planting outside the Amazon rainforest; however, these areas go through long periods of water scarcity and demand artificial irrigation to be sustainable. One-quarter of the irrigated area in agriculture has a problem with salinity stress. Tipo: Anais e Proceedings de eventos Palavras-chave: Abiotic stress; Transcription factors; Non-coding RNA; Oil palm products. Ano: 2022 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1149420 Single-cell qPCR facilitates the optimization of hematopoietic differentiation in hPSCs/OP9 coculture system BJMBR Chen,Haide; Jiang,Mengmeng; Xiao,Lei; Huang,He. Human pluripotent stem cells (hPSCs)/OP9 coculture system is a widely used hematopoietic differentiation approach. The limited understanding of this process leads to its low efficiency. Thus, we used single-cell qPCR to reveal the gene expression profiles of individual CD34+ cells from different stages of differentiation. According to the dynamic gene expression of hematopoietic transcription factors, we overexpressed specific hematopoietic transcription factors (Gata2, Lmo2, Etv2, ERG, and SCL) at an early stage of hematopoietic differentiation. After overexpression, we generated more CD34+ cells with normal expression level of CD43 and CD31, which are used to define various hematopoietic progenitors. Furthermore, these CD34+ cells possessed normal... Tipo: Info:eu-repo/semantics/article Palavras-chave: Single-cell qPCR; Human pluripotent stem cells; Hematopoietic differentiation; OP9-GFP; Transcription factors. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500608 The use of sunflower transcription factors as biotechnological tools to improve yield and stress tolerance in crops Phyton Chan,R.L. Transcription factors (TFs) are proteins able to specifically recognize DNA sequences in the regulatory regions of their target genes. They bind these specific sequences, an event that leads to the activation or repression of whole signal transduction pathways. In plants about 1500 TFs were informatically identified; identification was mainly based in the presence of DNA-binding domains in the translated sequences. They were classified in families and subfamilies according to several features, including the conservation of the DNA binding domain, the genes structures and the functions they exert. Among transcription factors, several seem to be potential powerful biotechnological tools to improve crops via obtaining transgenic plants. Assigned purposes... Tipo: Info:eu-repo/semantics/article Palavras-chave: Sunflower; Transcription factors; HD-Zip; Homeodomain; Leucine zipper; Abiotic stress; Biotic stress. Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572009000100002 Registros recuperados: 25 Primeira ... 1 2 ... Última

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