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A phage display combined with DNA affinity magnetic system can be applied to a screening of DNA binding proteins, such as transcription factors Electron. J. Biotechnol.
Yulita,Kusumadewi Sri; Kouno,Takafumi; Ezaki,Bunichi.
Here we introduce a new approach for the screening of DNA binding proteins, using a phage library based on a phage display technique. In principal, a complementary DNA (cDNA) library based on the recombinant bacteriophage T7 expressing target proteins on its capsid (phage display) is constructed. These phage particles are hybridized with a biotinylated target DNA fragment which is immobilized on the surface of streptavidin paramagnetic particle (SA-PMP). The phage particles are released from the target DNA fragment by a nuclease treatment and the recovered phages are used to the next round of hybridization. These processes are repeated three times to amplify the target phages in the population. This simple method is faster, and more systemic than other...
Tipo: Journal article Palavras-chave: AtGST11 gene; Biopanning; DNA binding proteins; T7 phage differential display; Transcription factors.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100014
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Agronomic evaluation of genetically modified soybean genotypes in response to water deficit. Repositório Alice
LIMA, L. F. S. de; FERREIRA, L. C.; FUGANTI-PAGLIARINI, R.; MARIN, S. R. R.; OLIVEIRA, M. C. N. de; MERTZ-HENNING, L. M.; FARIAS, J. R. B.; NEUMAIER, N.; NAKASHIMA, K.; NUNES, L. M.; NEPOMUCENO, A. L..
bitstream/item/188954/1/Lima-77.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Resistência a Seca; Soja; Fator de Crescimento; Soybeans; Drought tolerance; Transcription factors; Plant growth.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102092
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An effective method for cloning of partial MADS-box genes related to flower development in groundnut Open Agri
Yuan, M..
Palavras-chave: Genes; PCR; DNA; Cloning; Transcription factors; Genomes; Transcription; Genetic structures; Nucleotides; Molecular cloning.
Ano: 2005 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3213
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Analysis of the SHP2 enhancer for the use of tissue specific activation tagging in Arabidopsis thaliana Genet. Mol. Biol.
Chalfun-Junior,Antonio; Mes,Jurriaan J.; Busscher,Marco; Angenent,Gerco C..
Activation tagging is a powerful tool to identify new mutants and to obtain information about possible biological functions of the overexpressed genes. The quadruple cauliflower mosaic virus (CaMV) 35S enhancer fragment is a strong enhancer, which is most commonly used for this purpose. However, the constitutive nature of this enhancer may generate lethal mutations or aberrations in different plant organs by the same overexpressed gene. A tissue-specific activation tagging approach may overcome these drawbacks and may also lead more efficiently to the desired phenotype. For this reason the SHATTERPROOF2 (SHP2) promoter fragment was analysed for enhancer activity. The SHP2 gene is involved in dehiscence zone development and expressed during silique...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arabidopsis; Development; Pod shattering; Transcription factors; Mutants.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200032
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Analyzing gene expression profiles in dilated cardiomyopathy via bioinformatics methods BJMBR
Wang,Liming; Zhu,L.; Luan,R.; Wang,L.; Fu,J.; Wang,X.; Sui,L..
Dilated cardiomyopathy (DCM) is characterized by ventricular dilatation, and it is a common cause of heart failure and cardiac transplantation. This study aimed to explore potential DCM-related genes and their underlying regulatory mechanism using methods of bioinformatics. The gene expression profiles of GSE3586 were downloaded from Gene Expression Omnibus database, including 15 normal samples and 13 DCM samples. The differentially expressed genes (DEGs) were identified between normal and DCM samples using Limma package in R language. Pathway enrichment analysis of DEGs was then performed. Meanwhile, the potential transcription factors (TFs) and microRNAs (miRNAs) of these DEGs were predicted based on their binding sequences. In addition, DEGs were mapped...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dilated cardiomyopathy; Differentially expressed genes; Pathway enrichment analysis; Transcription factors; MicroRNAs; Small molecules.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000706
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Apoptosis induction by E2F decoy DNA of the prostate cancer cell line BABT
Wang,Tao; Jiang,An -li; Zhang,Jian-ye; Jing,Fei.
To observe effect of E2F decoy DNA on proliferation and apoptosis of androgen-independent prostate cancer cell line PC-3M the binding specificity of the E2F decoy DNA to the PC-3M nuclear protein was detected by electrophoretic mobility shift assay (EMSA). E2F decoy DNA, ARE decoy DNA and Control decoy DNA were respectively transfected into PC-3M cells with lipofectamine.Their influence on cell proliferative activity was detected by MTT assay.The cell apoptotic rate was determined by flow cytometric(FCM) analysis and chromosome DNA ladder was detected by DNA gel electrophoresis.The change of mRNA expression of C-myc and CyclinD1 were detected by RT-PCR.The change of mRNA expression of C-myc and CyclinD1 were detected by Western-blot. EMSA demonstrated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Decoy DNA; Transcription factors; PC-3M cells; Apoptosis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200011
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AtDREB2A-CA influences root architecture and increases drought tolerance in transgenic cotton. Repositório Alice
LISEI-DE-SÁ, M. E.; ARRAES, F. B. N.; BRITO, G. G.; BENEVENTI, M. A.; LOURENCO-TESSUTTI, I.; BASSO, A. M. M.; AMORIM, R. M. S.; SILVA, M. C. M.; FAHEEM, M.; OLIVEIRA, N. G.; MIZOI, J.; YAMAGUCHI-SHINOZAKI, K.; GROSSI-DE-SA, M. F..
bitstream/item/167271/1/AS-2017103015574695.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Dehydration Responsive Element Binding Factors; Water Deficit Tolerance; Physiological Phenotyping; Stress-Inducible Promoter; Gossypium hirsutum; Transcription factors.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080442
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Candidate genes and pathogenesis investigation for sepsis-related acute respiratory distress syndrome based on gene expression profile Biol. Res.
Wang,Min; Yan,Jingjun; He,Xingxing; Zhong,Qiang; Zhan,Chengye; Li,Shusheng.
BACKGROUND: Acute respiratory distress syndrome (ARDS) is a potentially devastating form of acute inflammatory lung injury as well as a major cause of acute respiratory failure. Although researchers have made significant progresses in elucidating the pathophysiology of this complex syndrome over the years, the absence of a universal detail disease mechanism up until now has led to a series of practical problems for a definitive treatment. This study aimed to predict some genes or pathways associated with sepsis-related ARDS based on a public microarray dataset and to further explore the molecular mechanism of ARDS RESULTS: A total of 122 up-regulated DEGs and 91 down-regulated differentially expressed genes (DEGs) were obtained. The up- and down-regulated...
Tipo: Journal article Palavras-chave: Acute respiratory distress syndrome; Sepsis; Differentially expressed mRNAs; Functional enrichment analysis; Pathway analysis; Transcription factors.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100025
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Cloning and Overexpression of GmDREB2 Gene from a Vietnamese Drought-resistant Soybean Variety BABT
Tan,Dao Xuan; Tuong,Ho Manh; Thuy,Vu Thi Thu; Son,Le Van; Mau,Chu Hoang.
ABSTRACTThis work studied the amplification, cloning and determination of the GmDREB2 gene from the soybean cultivar DT2008 and five Vietnamese local soybean cultivars (DT26, DT51, DVN5, CB, CBD) and designed the vector carrying the structure containing GmDREB2 gene from cultivar DT2008 (best drought tolerant). The coding region of GmDREB2 gene isolated from six soybean cultivars was 480 nucleotides in length, encoding 159 amino acids. The recombinant structure was designed as 35S- GmDREB2- c-myc and its expression was analysed in transgenic tobacco plants. Recombinant DREB2 protein was expressed in five transgenic tobacco lines with molecular weights close to 20 kDa. During the drought conditions, the proline accumulation of the transgenic tobacco lines...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drought-resistant; GmDREB2 gene; Gene transfer; Soybean; Transcription factors.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500651
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Differential impact of acclimation and acute water deprivation in the expression of key transcription factors in soybean roots. Repositório Alice
GUIMARÃES-DIAS, F.; NEVES-BORGES, A. C.; CONFORTE, A. J.; GIOVANELLA-KAMPMANN, L.; FERREIRA, A. V. J.; AMORIM, R. M. S.; BENEVENT, M. A.; SÁ, M. E. L. de; MESQUITA, R. O.; RODRIGUES, F. A.; NEPOMUCENO, A. L.; ROMANO, E.; LOUREIRO, M. E.; GROSSI-DE-SA, M. F.; ALVES-FERREIRA, M..
bitstream/item/180696/1/Guimaraes-Dias2016-Article-DifferentialImpactOfAcclimatio.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Drought adaptation; Marker assistance selection; Water deficit; Glycine Max; Transcription factors.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1056209
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DREB1A allows for more water uptake in groundnut by a large modification in the root/shoot ratio under water deficit Open Agri
Vadez, V..
Palavras-chave: Transgenics; Evapotranspiration; Drought stress; Genotypes; Nutrient uptake; Transgenic plants; Transpiration (animals); Harvest index; Transcription; Transcription factors.
Ano: 2007 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3355
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Overall picture of expressed Heat Shock Factors in Glycine max, Lotus japonicus and Medicago truncatula. Repositório Alice
SOARES-CAVALCANTI, N. M.; BELARMINO, L. C.; KIDO, E. A.; PANDOLFI, V.; MARCELINO-GUIMARÃES, F. C.; RODRIGUES, F. A.; PEREIRA, G. A. G.; BENKO-ISEPPON, A. M..
Heat shock (HS) leads to the activation of molecular mechanisms, known as HS-response, that prevent damage and enhance survival under stress. Plants have a flexible and specialized network of Heat Shock Factors (HSFs), which are transcription factors that induce the expression of heat shock proteins. The present work aimed to identify and characterize the Glycine max HSF repertory in the Soybean Genome Project (GENOSOJA platform), comparing them with other legumes (Medicago truncatula and Lotus japonicus) in view of current knowledge of Arabidopsis thaliana. The HSF characterization in leguminous plants led to the identification of 25, 19 and 21 candidate ESTs in soybean, Lotus and Medicago, respectively. A search in the SuperSAGE libraries revealed 68...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bioinformática; Soja; Gene; Genoma; Bioinformatics; Soybeans; Genome; Genes; Abiotic stress; Transcription factors.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/926601
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Possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cells Biol. Res.
JAIMOVICH,ENRIQUE; ESPINOSA,ALEJANDRA.
We studied the effect of IGF-I, insulin and testosterone on intracellular Ca2+ in cultured muscle cells. Insulin produced a fast (<1 s) and transient [Ca2+] increase lasting less than 10 s. IGF-I induced a transient [Ca2+] increase, reaching a fluorescence peak 6 s after stimulus, to return to basal values after 60 s. Testosterone induced delayed (35 s) and long lasting (100-200 s) signals, frequently associated with oscillations. IGF-I, testosterone and electrical stimulation-induced Ca2+ signals were shown to be dependent on IP3 production. All of these Ca2+ signals were blocked by inhibitors of the IP3 pathway. On the other hand, insulin-induced Ca2+ increase was dependent on ryanodine receptors and blocked by either nifedipine or ryanodine. The...
Tipo: Journal article Palavras-chave: Skeletal; Transcription factors; Insulin; IGF-I; Testosterone.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400018
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Single-cell qPCR facilitates the optimization of hematopoietic differentiation in hPSCs/OP9 coculture system BJMBR
Chen,Haide; Jiang,Mengmeng; Xiao,Lei; Huang,He.
Human pluripotent stem cells (hPSCs)/OP9 coculture system is a widely used hematopoietic differentiation approach. The limited understanding of this process leads to its low efficiency. Thus, we used single-cell qPCR to reveal the gene expression profiles of individual CD34+ cells from different stages of differentiation. According to the dynamic gene expression of hematopoietic transcription factors, we overexpressed specific hematopoietic transcription factors (Gata2, Lmo2, Etv2, ERG, and SCL) at an early stage of hematopoietic differentiation. After overexpression, we generated more CD34+ cells with normal expression level of CD43 and CD31, which are used to define various hematopoietic progenitors. Furthermore, these CD34+ cells possessed normal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Single-cell qPCR; Human pluripotent stem cells; Hematopoietic differentiation; OP9-GFP; Transcription factors.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500608
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The use of sunflower transcription factors as biotechnological tools to improve yield and stress tolerance in crops Phyton
Chan,R.L.
Transcription factors (TFs) are proteins able to specifically recognize DNA sequences in the regulatory regions of their target genes. They bind these specific sequences, an event that leads to the activation or repression of whole signal transduction pathways. In plants about 1500 TFs were informatically identified; identification was mainly based in the presence of DNA-binding domains in the translated sequences. They were classified in families and subfamilies according to several features, including the conservation of the DNA binding domain, the genes structures and the functions they exert. Among transcription factors, several seem to be potential powerful biotechnological tools to improve crops via obtaining transgenic plants. Assigned purposes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sunflower; Transcription factors; HD-Zip; Homeodomain; Leucine zipper; Abiotic stress; Biotic stress.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572009000100002
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Towards the identification of flower-specific genes in Citrus spp Genet. Mol. Biol.
Dornelas,Marcelo Carnier; Camargo,Raquel Luciana Boscariol; Berger,Irving Joseph; Takita,Marco Aurélio.
Citrus sinensis is a perennial woody species, for which genetic approaches to the study of reproductive development are not readily amenable. Here, the usefulness of the CitEST Expressed Sequence Tag (EST) database is demonstrated as a reliable new resource for identifying novel genes exclusively related to Citrus reproductive biology. We performed the analysis of an EST dataset of the CitEST Project containing 4,330 flower-derived cDNA sequences. Relying on bioinformatics tools, sequences exclusively present in this flower-derived sequence collection were selected and used for the identification of Citrus putative flower-specific genes. Our analysis revealed several Citrus sequences showing significant similarity to conserved genes known to have...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Development; Flower-specific genes; Reproduction; Transcription factors.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500005
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Transcription factors in microalgae: genome-wide prediction and comparative analysis ArchiMer
Thiriet-rupert, Stanislas; Carrier, Gregory; Chenais, Benoit; Trottier, Camille; Bougaran, Gael; Cadoret, Jean-paul; Schoefs, Benoit; Saint-jean, Bruno.
Background: Studying transcription factors, which are some of the key players in gene expression, is of outstanding interest for the investigation of the evolutionary history of organisms through lineage-specific features. In this study we performed the first genome-wide TF identification and comparison between haptophytes and other algal lineages. Results: For TF identification and classification, we created a comprehensive pipeline using a combination of BLAST, HMMER and InterProScan software. The accuracy evaluation of the pipeline shows its applicability for every alga, plant and cyanobacterium, with very good PPV and sensitivity. This pipeline allowed us to identify and classified the transcription factor complement of the three haptophytes...
Tipo: Text Palavras-chave: Algae; Endosymbiotic gene transfer; Haptophytes; Prediction pipeline; Stramenopiles; Tisochrysis lutea; Transcription factors.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00333/44403/44072.pdf
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Transcriptional regulators and regulatory pathways involved in prostate gland adaptation to a hypoandrogen environment Genet. Mol. Biol.
Nishan,Umar; da Rosa-Ribeiro,Rafaela; Damas-Souza,Danilo Marchete; Barbosa,Guilherme Oliveira; Carvalho,Hernandes F..
Abstract Anti-androgen therapies, including orchiectomy, are effective at promoting prostate cancer remission, but are followed by progression to the more aggressive castration-resistant prostate cancer (CRPC). Castration promotes gland and tumor shrinkage. However, prostate adaptation to androgen deprivation involves striking parallel events, all requiring changes in gene expression. We hypothesized that transcription factors (TF) and other transcription-related genes are needed to orchestrate those changes. In this work, downstream analysis using bioinformatic tools and published microarray data allowed us to identify sixty transcriptional regulators (including 10 TF) and to integrate their function in physiologically relevant networks. Functional...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Androgens; Arnt; Castration; Prostate; Transcription factors.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500802
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Transcritos de genes de defensa acumulados en chile CM334 infectado por Nacobbus aberrans o por Meloidogyne enterolobii e inoculado con Phytophthora capsici. Colegio de Postgraduados
Villar Luna, Edgar.
El chile CM334 es resistente al oomiceto Phytophthora capsici (Pc) y a los nematodos Meloidogyne incognita, M. arenaria y M. javanica, pero susceptible a M. enterolobii (Me) y Nacobbus aberrans (Na). La resistencia de Capsicum annuum a Pc se asocia con la acumulación de la fitoalexina sesquiterpenica capsidiol y con la sobre-expresión de varios genes de defensa. Los objetivos de este trabajo fueron: 1) conocer las modificaciones que Na y Me inducen en la expresión de algunos genes de la ruta mevalónica (HMG2, EAS, HMG3, y SS) en plantas CM334 infectadas por Na (NaPc) o Me (MPc) e inoculadas con Pc (Pc); 2) determinar la relación entre los niveles de HMG2 y EAS en raíces de CM334 inoculadas sólo con Na (N), con Pc (Pc) y con ambos patógenos (NPc); y 3)...
Palavras-chave: Capsicum annuum; Nematodos agalladores; QRT-PCR; Factores de transcripción; Ruta mevalónica; Root-knot nematodes; Transcription factors; Mevalonate pathway; Fitopatología; Doctorado.
Ano: 2013 URL: http://hdl.handle.net/10521/2113
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Variantes da região promotora do gene KCNJ11 em bovinos da raça Nelore. Repositório Alice
OLIVEIRA, K. S. de; LIMA, A. O. de; AFONSO, J.; MELO, A. L. de; REGITANO, L. C. de A..
A eficiência alimentar é uma característica moderadamente herdável, que por possuir uma mensuração onerosa e tardia, tem sua incorporação em programas de melhoramento animal dificultada. Estudos prévios de mapeamento identificaram o gene KCNJ7 7, que possui importante função sobre vias metabólicas relacionadas a eficiência alimentar, tais como a via de insulina, em uma região de QTL (quantitative trait loci) para maciez da carne.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Sítios-alvo de fator de transcrição; Gado Nelore; Melhoramento Genético Animal; Bos Indicus; Animal breeding; Transcription factors.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1095987
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