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Registros recuperados: 18
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Active transcription and ultrastructural changes during Trypanosoma cruzi metacyclogenesis Anais da ABC (AABC)
Ferreira,Ludmila R.P.; Dossin,Fernando de M.; Ramos,Thiago C.; Freymüller,Edna; Schenkman,Sergio.
The differentiation of proliferating epimastigote forms of Trypanosoma cruzi , the protozoan parasite that causes Chagas’ disease, into the infective and non-proliferating metacyclic forms can be reproduced in the laboratory by incubating the cells in a chemically-defined medium that mimics the urine of the insect vector. Epimastigotes have a spherical nucleus, a flagellum protruding from the middle of the protozoan cell, and a disk-shaped kinetoplast - an organelle that corresponds to the mitochondrial DNA. Metacyclic trypomastigotes have an elongated shape with the flagellum protruding from the posterior portion of the cell and associated with a spherical kinetoplast. Here we describe the morphological events of this transformation and characterize a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trypanosoma cruzi; Metacyclogenesis; Transcription; RNA polymerase II.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652008000100011
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Alternative cytoplasmic male sterility systems in sorghum and their utilization Open Agri
A.A. ( A. Ashok Kumar ).
Palavras-chave: Cytoplasm; Genes; Sorghum; Hybrids; Heterosis; Transcription; DNA; Genetic structures; Segregation; Genomes.
Ano: 2008 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3149
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An effective method for cloning of partial MADS-box genes related to flower development in groundnut Open Agri
Yuan, M..
Palavras-chave: Genes; PCR; DNA; Cloning; Transcription factors; Genomes; Transcription; Genetic structures; Nucleotides; Molecular cloning.
Ano: 2005 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3213
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Diverse coactivator recruitment through differential PPARγ nuclear receptor agonism Genet. Mol. Biol.
Lizcano,Fernando; Vargas,Diana.
The PPARγ nuclear receptor regulates the expression of genes involved in lipid and carbohydrate metabolism, and it has protective effects in some patients with type 2 diabetes. Nevertheless, the therapeutic value of the PPARγ nuclear receptor protein is limited due to the secondary effects of some PPARγ ligands. Because the downstream effects of PPARγ are determined by the binding of specific cofactors that are mediated by ligand-induced conformational changes, we evaluated the differential effects of various ligands on the binding of certain cofactors associated with PPARγ. The ligands used were rosiglitazone for treating type 2 diabetes and telmisartan for treating arterial hypertension. Functional, phenotypic, and molecular studies were conducted on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA binding proteins co-activator; Gene expression; Transcription; Telmisartan.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100020
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DREB1A allows for more water uptake in groundnut by a large modification in the root/shoot ratio under water deficit Open Agri
Vadez, V..
Palavras-chave: Transgenics; Evapotranspiration; Drought stress; Genotypes; Nutrient uptake; Transgenic plants; Transpiration (animals); Harvest index; Transcription; Transcription factors.
Ano: 2007 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3355
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Expressão de genes da subfamília HD-Zip I em soja submetida à seca. Repositório Alice
PEREIRA, A.A.; MORALES, A.M.A.P.; BORÉM, A.; LOREIRO, M.E..
O objetivo deste trabalho foi identificar genes candidatos da subfamília de fatores transcricionais HD-Zip I que contribuem para a tolerância à seca em soja. Foram avaliados trifólios de soja de cultivar tolerante (Embrapa 48) e suscetível à seca (BR 16), sob três níveis de deficit hídrico: ausência, moderado (-1,5 MPa) e severo (-3,0 MPa). Pela análise dos promotores, foi identificada a presença de possíveis elementos cis-regulatórios relacionados à resposta à seca, nos três genes avaliados (GmHB6, GmHB13 e GmHB21). No entanto, não houve padrão de distribuição específico associado à maior tolerância do genótipo à seca. Com a análise comparativa, foram identificados seis elementos cis-regulatórios potencialmente envolvidos na indução da expressão gênica...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Elementos cis-regulatórios; Fatores transcricionais; Genômica; PCR em tempo real. cis-regulatory elements; Transcription.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/908417
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Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements Genet. Mol. Biol.
Liang,Qiaoyi; Ding,Jiayi; Zheng,Shu.
Up-regulation of human endogenous retroviruses (HERVs) is associated with many diseases, including cancer. In this study, an H family HERV (HERV-H)-related gene was identified and characterized. Its spliced transcript lacks protein-coding capacity and may belong to the emerging class of noncoding RNAs (ncRNAs). The 1.3-kb RNA consisting of four exons is transcribed from an Alu element upstream of a 5.0-kb structurally incomplete HERV-H element. RT-PCR and quantitative RT-PCR results indicated that expression of this HERV-related transcript was negatively associated with colon, stomach, and kidney cancers. Its expression was induced upon treatment with DNA methylation and histone deacetylation inhibitors. A BLAT search using long terminal repeats (LTRs)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endogenous retrovirus; Noncoding RNA; RT-PCR; Quantitative real-time PCR; Transcription.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400005
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Identification of drought tolerant groundnut genotypes employing proteomics approach Open Agri
Ramesh Katam.
Palavras-chave: Drought stress; Genotypes; Weather hazards; Mycotoxins; Transcription; Tolerance; Proteins; Cytochromes; Photosystems; Gene expression.
Ano: 2007 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3361
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In silico analysis of ESTs from roots of Rangpur lime (Citrus limonia Osbeck) under water stress Genet. Mol. Biol.
Boscariol-Camargo,Raquel L.; Berger,Irving J.; Souza,Alessandra A.; Amaral,Alexandre M. do; Carlos,Eduardo F.; Freitas-Astúa,Juliana; Takita,Marco A.; Targon,Maria Luisa P.N.; Medina,Camilo L.; Reis,Marcelo S.; Machado,Marcos A..
CitEST project resulted in the construction of cDNA libraries from different Citrus sp. tissues under various physiological conditions. Among them, plantlets of Rangpur lime were exposed to hydroponic conditions with and without water stress using PEG6000. RNA from roots was obtained and generated a total of 4,130 valid cDNA reads, with 2,020 from the non-stressed condition and 2,110 from the stressed set. Bioinformatic analyses measured the frequency of each read in the libraries and yielded an in silico transcriptional profile for each condition. A total of 40 contigs were differentially expressed and allowed to detect up-regulated homologue sequences to well known genes involved in stress response, such as aquaporins, dehydrin, sucrose synthase, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Expressed sequence tags; Transcription; Rootstock; Drought; Molecular response.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500019
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Modulation of thyroid hormone receptor transactivation by the early region 1A (E1A)-like inhibitor of differentiation 1 (EID1) Genet. Mol. Biol.
Vargas,Diana; Celis,Luis; Romero,Carolina; Lizcano,Fernando.
Transcriptional activation (TA) mediated by the effect of thyroid hormones on target genes requires co-activator proteins such as the early region 1A (E1A) associated 300 kDa binding protein (p300) and the cAMP response element binding protein (CREB) binding protein (CBP), known as the p300/CBP complex, which acetylate histones 3 and 4 to allow transcriptional machinery access to the target gene promoter. Little is known on the role of p300 in thyroid hormone receptor (TR) mediated TA but the E1A-like inhibitor of differentiation 1 (EID1), an inhibitor of p300 histone acetyltransferase (HAT), is a functional homolog of E1A and may inhibit myogenic differentiation factor D (MyoD) transcriptional activity and reduces muscle cell differentiation. We evaluated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Co-activator; EID1; P300; Thyroid receptors; Transcription.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300003
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Perfil transcricional em escala genômica de genes associados a respostas à temperatura em videira. Repositório Alice
2015
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Hormone; Transcription; Climate; Electron microscopy.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1036600
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Polymorphisms in promoter sequences of MDM2, p53, and p16INK4a genes in normal Japanese individuals Genet. Mol. Biol.
Ohsaka,Yasuhito; Nishino,Hoyoku.
Research has been conducted to identify sequence polymorphisms of gene promoter regions in patients and control subjects, including normal individuals, and to determine the influence of these polymorphisms on transcriptional regulation in cells that express wild-type or mutant p53. In this study we isolated genomic DNA from whole blood of healthy Japanese individuals and sequenced the promoter regions of the MDM2, p53, and p16INK4a genes. We identified polymorphisms comprising 3 nucleotide substitutions at exon 1 and intron 1 regions of the MDM2 gene and 1 nucleotide insertion at a poly(C) nucleotide position in the p53 gene. The Japanese individuals also exhibited p16INK4a polymorphisms at several positions, including position -191. Reporter gene analysis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Murine double minute 2; Polymorphism; P16INK4a; P53; Transcription.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400004
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Regulation of gene expression in Mycoplasmas: contribution from Mycoplasma hyopneumoniae and Mycoplasma synoviae genome sequences Genet. Mol. Biol.
Madeira,Humberto Maciel França; Gabriel,Jane Eyre.
This report describes the transcription apparatus of Mycoplasma hyopneumoniae (strains J and 7448) and Mycoplasma synoviae, using a comparative genomics approach to summarize the main features related to transcription and control of gene expression in mycoplasmas. Most of the transcription-related genes present in the three strains are well conserved among mycoplasmas. Some unique aspects of transcription in mycoplasmas and the scarcity of regulatory proteins in mycoplasma genomes are discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycoplasma hyopneumoniae; Mycoplasma synoviae; Transcription; Comparative genomics.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200016
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Regulation of transgene expression in genetic immunization BJMBR
Harms,J.S.; Oliveira,S.C.; Splitter,G.A..
The use of mammalian gene expression vectors has become increasingly important for genetic immunization and gene therapy as well as basic research. Essential for the success of these vectors in genetic immunization is the proper choice of a promoter linked to the antigen of interest. Many genetic immunization vectors use promoter elements from pathogenic viruses including SV40 and CMV. Lymphokines produced by the immune response to proteins expressed by these vectors could inhibit further transcription initiation by viral promoters. Our objective was to determine the effect of IFN-<FONT FACE="Symbol">g</FONT> on transgene expression driven by viral SV40 or CMV promoter/enhancer and the mammalian promoter/enhancer for the major...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA vaccine; Gene therapy; Regulation; Transcription; Transgene.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200003
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Signal transduction and gene expression regulated by calcium release from internal stores in excitable cells Biol. Res.
CARRASCO,MARÍA ANGÉLICA; JAIMOVICH,ENRIQUE; KEMMERLING,ULRIKE; HIDALGO,CECILIA.
Calcium regulation of several transcription factors involves different calcium-dependent signaling cascades and engages cytoplasmic as well as nuclear calcium signals. The study of the specific sources of calcium signals involved in regulation of gene expression in skeletal muscle has been addressed only recently. In this tissue, most cytoplasmic and nuclear calcium signals originate from calcium release from internal stores, mediated either by ryanodine receptor (RyR) or IP3 receptor (IP3R) channels. The latter are located both in the sarcoplasmic reticulum (SR) and in the nuclear membrane, and their activation results in long-lasting nuclear calcium increase. The calcium signals mediated by RyR and IP3R are very different in kinetics, amplitude and...
Tipo: Journal article Palavras-chave: Calcium signals; Intracellular stores; Transcription; Oxidation; Neurons; Skeletal muscle.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400028
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The nucleolus: a paradigm for cell proliferation and aging BJMBR
Comai,L..
The nucleolus is the cellular site of ribosome biosynthesis. At this site, active ribosomal DNA (rDNA) genes are rapidly transcribed by RNA polymerase I (pol I) molecules. Recent advances in our understanding of the pol I transcription system have indicated that regulation of ribosomal RNA (rRNA) synthesis is a critical factor in cell growth. Importantly, the same signaling networks that control cell growth and proliferation and are deregulated in cancer appear to control pol I transcription. Therefore, the study of the biochemical basis for growth regulation of pol I transcription can provide basic information about the nuclear signaling network. Hopefully, this information may facilitate the search for drugs that can inhibit the growth of tumor cells by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nucleolus; RNA pol I; Aging; Transcription; Werner syndrome.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200004
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Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell lines Genet. Mol. Biol.
Yamada,Hiroya; Takano,Toru; Matsuzuka,Fumio; Watanabe,Mikio; Miyauchi,Akira; Iwatani,Yoshinori.
Thyroid transcription factor-1 (TTF-1, NKX2-1) is a homeodomain-containing transcriptional factor that binds to and activates the promoters of thyroid and lung-specific genes, such as thyroglobulin, thyroid peroxidase, and thyroid stimulating hormone receptor. TTF-1 is known to play a key role in the development of the thyroid. However, the precise mechanism of TTF-1 gene transcription in human thyroid cells has not been studied. The expression of transcriptional activity in various lengths of the 5'-flanking region of the human TTF -1 gene was studied in TTF-1 positive and negative human thyroid cell lines. Increased transcriptional activity was observed in thyroid cell lines containing plasmids that coded for a sequence proximal to the transcription...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thyroid; TTF-1; Transcription; Human cell line; Stem cell.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100002
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การวิเคราะห์ข้อมูลการถอดรหัส ที่ส่งผลต่อการแสดงออกของยีนที่แตกต่างกันในช่อดอกจากจีโนมข้าวลูกผสมและพันธุ์พ่อแม่ Thai Agricultural
Kulchana Ketsuwan.
Next Generation Sequencing (NGS): SOLiD System Sequencing Platform was applied to investigate the whole transcriptome of two hybrids (PTT06001H; H1 and PTT06008H: H2), two paternal lines (CK81; P1 and CK120; P2), and maternal line (IR79156; M). The data sets were aligned against the reference, precisely, the total numbers of the individual rice panicles sample aligned with Nipponbare ranking from the highest were as follows, P2 (9,747,077 reads), H1 (7,386,061 reads) , P1 (5,361,077 reads) , H2 (5,196,982 reads), and M (2,244,306 reads), respectively. The differentially expressed genes (DEGs) were identified and found that 2902, 1604, 3168, 3068, 1171, and 3031 genes were up-regulated (> 2 fold) between M vs. H1, P1 vs. H1, M vs.P1, M vs. H2, P2 vs. H2,...
Tipo: PhysicalObject Palavras-chave: Hybrid rice; Heterosis; Differentially expressed genes; DEGs; Genetic transformation; Gene expression; Transcription; Nucleotide analysis; Plant breeding; Rice productivity; ข้าว; พันธุ์ลูกผสม; ช่อดอก; การถ่ายทอดทางพันธุกรรม; การแสดงออกของยีน; การถอดรหัสพันธุกรรม; การวิเคราะห์นิวคลีโอไทด์; การปรับปรุงพันธุ์; ศักยภาพความดีเด่นเหนือพ่อแม่; การเพิ่มผลผลิต.
Ano: 2014 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5659
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