|
|
|
| Registros recuperados: 76 | |
|
|
| Sussarellu, Rossana; Huvet, Arnaud; Lapegue, Sylvie; Quillen, Virgile; Lelong, Christophe; Cornette, Florence; Jensen, Lasse Fast; Bierne, Nicolas; Boudry, Pierre. |
| Background Originating from Northeast Asia, the Pacific oyster Crassostrea gigas has been introduced into a large number of countries for aquaculture purpose. Following introduction, the Pacific oyster has turned into an invasive species in an increasing number of coastal areas, notably recently in Northern Europe. Methods To explore potential adaptation of reproductive traits in populations with different histories, we set up a common garden experiment based on the comparison of progenies from two populations of Pacific oyster sampled in France and Denmark and their hybrids. Sex ratio, condition index and microarray gene expression in gonads, were analyzed in each progeny (n = 60). Results A female-biased sex-ratio and a higher condition index were... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Adaptation; Invasiveness; Fertility; Sex-ratio; Transcriptome; Microarray. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00287/39829/38343.pdf |
| |
|
| |
|
|
| PASSOS, M. A. N.; CRUZ, V. O. de; EMEDIATO, F. L.; TEIXEIRA, C. C. de; AZEVEDO, V. C. R.; BRASILEIRO, A. C. M.; AMORIM, E. P.; FERREIRA, C. F.; MARTINS, N. F.; TOGAWA, R. C.; PAPPAS JÚNIOR, G. J.; SILVA JUNIOR, O. B. da; MILLER, R. T N. G.. |
| Background: Although banana (Musa sp.) is an important edible crop, contributing towards poverty alleviation and food security, limited transcriptome datasets are available for use in accelerated molecular-based breeding in this genus. 454 GS-FLX Titanium technology was employed to determine the sequence of gene transcripts in genotypes of Musa acuminata ssp. burmannicoides Calcutta 4 and M. acuminata subgroup Cavendish cv. Grande Naine, contrasting in resistance to the fungal pathogen Mycosphaerella musicola, causal organism of Sigatoka leaf spot disease. To enrich for transcripts under biotic stress responses, full length-enriched cDNA libraries were prepared from whole plant leaf materials, both uninfected and artificially challenged with pathogen... |
| Tipo: Separatas |
Palavras-chave: Banana; Fungo; Musa Acuminata; Mycosphaerella Musicola; Transcriptome; Microsatellite repeats. |
| Ano: 2013 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/964212 |
| |
|
| |
|
| |
|
|
| Vieira,Luiz Gonzaga Esteves; Andrade,Alan Carvalho; Colombo,Carlos Augusto; Moraes,Ana Heloneida de Araújo; Metha,Ângela; Oliveira,Angélica Carvalho de; Labate,Carlos Alberto; Marino,Celso Luis; Monteiro-Vitorello,Claúdia de Barros; Monte,Damares de Castro; Giglioti,Éder; Kimura,Edna Teruko; Romano,Eduardo; Kuramae,Eiko Eurya; Lemos,Eliana Gertrudes Macedo; Almeida,Elionor Rita Pereira de; Jorge,Érika C.; Albuquerque,Érika V. S.; Silva,Felipe Rodrigues da; Vinecky,Felipe; Sawazaki,Haiko Enok; Dorry,Hamza Fahmi A.; Carrer,Helaine; Abreu,Ilka Nacif; Batista,João A. N.; Teixeira,João Batista; Kitajima,João Paulo; Xavier,Karem Guimarães; Lima,Liziane Maria de; Camargo,Luis Eduardo Aranha de; Pereira,Luiz Filipe Protasio; Coutinho,Luiz Lehmann; Lemos,Manoel Victor Franco; Romano,Marcelo Ribeiro; Machado,Marcos Antonio; Costa,Marcos Mota do Carmo; Sá,Maria Fátima Grossi de; Goldman,Maria Helena S.; Ferro,Maria Inês T.; Tinoco,Maria Laine Penha; Oliveira,Mariana C.; Van Sluys,Marie-Anne; Shimizu,Milton Massao; Maluf,Mirian Perez; Eira,Mirian Therezinha Souza da; Guerreiro Filho,Oliveiro; Arruda,Paulo; Mazzafera,Paulo; Mariani,Pilar Drummond Sampaio Correa; Oliveira,Regina L.B.C. de; Harakava,Ricardo; Balbao,Silvia Filippi; Tsai,Siu Mui; Mauro,Sonia Marli Zingaretti di; Santos,Suzana Neiva; Siqueira,Walter José; Costa,Gustavo Gilson Lacerda; Formighieri,Eduardo Fernandes; Carazzolle,Marcelo Falsarella; Pereira,Gonçalo Amarante Guimarães. |
| Coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. The genus Coffea belongs to the Rubiaceae family which includes other important plants. The genus contains about 100 species but commercial production is based only on two species, Coffea arabica and Coffea canephora that represent about 70 % and 30 % of the total coffee market, respectively. The Brazilian Coffee Genome Project was designed with the objective of making modern genomics resources available to the coffee scientific community, working on different aspects of the coffee production chain. We have single-pass sequenced a total of 214,964 randomly picked clones from 37 cDNA libraries of C. arabica, C. canephora and C. racemosa,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea; CDNA; EST; Transcriptome. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100008 |
| |
|
| |
|
|
| OLIVEIRA, J. C. de; SILVA, L. M. da; FORMIGHIERI, E. F.; SILVA, C. C. da; SOUZA, A. P. de; CAMPOS, T. de. |
| O amendoim forrageiro tem ganhado cada vez mais importância devido às vantagens associadas ao seu uso. Entretanto, a quantidade de microssatélites disponíveis para a espécie ainda é restrita, o que tem sido um gargalo no avanço do programa de melhoramento. Nesse sentido, o objetivo deste trabalho foi desenvolver e caracterizar novos microssatélites a partir do transcriptoma de folhas de Arachis pintoi. Foram testados 186 locos em 19 acessos. Os locos com os melhores perfis de amplificação (64) foram selecionados para avaliação de polimorfismo, dos quais 63 (98,4%) apresentaram perfis polimórficos, com média de 7,37 alelos por loco. Os valores médios de heterozigosidade esperada (HE) e observada (HO) foram 0,72 e 0,31, respectivamente. Os marcadores... |
| Tipo: Parte de livro |
Palavras-chave: Amendoim forrageiro; Forage peanut; Cacahuetes forrajeros; Transcriptoma da folha; Fitomejoramiento; Leguminosas forrajeras; Repeticiones de microsatélite; Hojas; Variación genética; Conservación del germoplasma; Embrapa Acre; Rio Branco (AC); Acre; Amazônia Ocidental; Western Amazon; Amazonia Occidental; Melhoramento Genético Vegetal; Leguminosa Forrageira; Marcador Genético; Variação Genética; Banco de Germoplasma; Plant breeding; Forage legumes; Arachis pintoi; Genetic markers; Microsatellite repeats; Transcriptome; Leaves; Genetic variation; Germplasm conservation. |
| Ano: 2022 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1145988 |
| |
|
|
| OLIVEIRA, J. C. de; FORMIGHIERI, E. F.; GARCIA, A. L. B.; MARGARIDO, G. R. A.; CAMPOS, T. de. |
| O uso do amendoim forrageiro em consórcios com gramíneas nas pastagens e como cobertura verde, consorciado com culturas comerciais, tem crescido nos últimos anos. A análise do genoma funcional permite a identificação de genes de interesse agronômico. Assim, o objetivo deste trabalho foi realizar a anotação funcional de genes do transcriptoma de folhas de Arachis pintoi. Dos 98.432 transcritos analisados, 69% apresentaram correspondências com o banco de dados de proteínas do National Center of Biotechnology Information. As classes função molecular (36%) e processo biológico (35,8%) representaram a maioria dos termos de Gene Ontology atribuídos, enquanto o componente celular (28,2%) apresentou menor número. A análise de expressão diferencial identificou... |
| Tipo: Artigo em anais e proceedings |
Palavras-chave: Amendoim forrageiro; Forage peanut; Cacahuetes forrajeros; Anotação funcional; RNA-seq; Transcriptoma; Hojas; Amarillo; Belomonte; Genoma; Folha; Método de Análise; Arachis pintoi; Genome; Transcriptome; Leaves. |
| Ano: 2023 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1157482 |
| |
|
|
| FAJARDO, T. V. M.; QUECINI, V.. |
| Key message The transcriptomes of wild and cultivated grapes consists of similar expressed genes but distinct wiring of co-expressed modules associated with environmental conditions. Abstract Grapevine is an important fruit crop worldwide, with high economic value and widespread distribution. Commercial production is based on Vitis vinifera, and, to a lesser extent, on hybrids with American grapes, such as V. labrusca. Wild grape relatives are important sources of resistance against biotic and abiotic factors; however, their global gene expression patterns remain poorly characterized. We associated genome-wide transcript profling to phenotypic analyses to investigate the responses of cultivated and wild vines to vineyard conditions. The expressed genes in... |
| Tipo: Artigo de periódico |
Palavras-chave: Environmental conditions; Grapevine; Network analysis; Parthenocissus; Phenology; Transcriptome; Vitaceae. |
| Ano: 2021 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1129823 |
| |
|
| |
|
|
| PADILHA, L.; CAIXETA, E. T.; SILVA, F. R. da. |
| New sequencing technologies brought deep probing of transcriptomes (so-called RNAseq) to the reach of individual researches. Analysis of RNAseq sequences, however, depend on the alignment of reads to a reference genome. Some approaches have been proposed to allow de novo assembly of the transcripts, therefore allowing RNAseq to be used on organisms lacking a reference genome. The efficiency of those approaches, however, were tested only with diploid species. Here we propose a methodology to evaluate the results of de novo assembly of allotetraploid Coffea arabica RNAseq reads obtained from libraries of coffee leaves infected by Hemileia vastatrix. Trinity was able to assemble longer transcripts when compared to ABySS, SOAPdenovo and Oases. Moreover, the... |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: Coffea Arábica; Genoma; Transcriptome. |
| Ano: 2012 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126751 |
| |
|
|
| Benzekri, Hicham; Armesto, Paula; Cousin, Xavier; Rovira, Mireia; Crespo, Diego; Alejandro Merlo, Manuel; Mazurais, David; Bautista, Rocio; Guerrero-fernandez, Dario; Fernandez-pozo, Noe; Ponce, Marian; Infante, Carlos; Zambonino, Jose Luis; Nidelet, Sabine; Gut, Marta; Rebordinos, Laureana; Planas, Josep V.; Begout, Marie-laure; Gonzalo Claros, M.; Manchado, Manuel. |
| Background Senegalese sole (Solea senegalensis) and common sole (S. solea) are two economically and evolutionary important flatfish species both in fisheries and aquaculture. Although some genomic resources and tools were recently described in these species, further sequencing efforts are required to establish a complete transcriptome, and to identify new molecular markers. Moreover, the comparative analysis of transcriptomes will be useful to understand flatfish evolution. Results A comprehensive characterization of the transcriptome for each species was carried out using a large set of Illumina data (more than 1,800 millions reads for each sole species) and 454 reads (more than 5 millions reads only in S. senegalensis), providing coverages ranging from... |
| Tipo: Text |
Palavras-chave: Soles; Transcriptome; Microarray; Orthology; Molecular markers; SoleaDB. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00238/34918/33626.pdf |
| |
|
|
| Fu,Suhong; Lei,Ming; Zhang,Yongqun; Deng,Zhaomin; Shi,Jing; Hao,Doudou. |
| Abstract Dysphania schraderiana is widely distributed in Lhasa (Tibet, China) and used as a traditional medicine. However, the lack of genetic information hinders the understanding of its physiological processes, such as the biosynthesis of secondary metabolites. Herein, we used Illumina Hiseq4000 platform to sequence the transcriptome of flower and leaf tissues from D. schraderiana for the first time. Totally, 40,142 unigenes were assembled from approximately 5.2 million clean reads. All unigenes underwent gene prediction and were subsequently annotated in a NR (NCBI non-redundant protein) database, COG (Clusters of Orthologous Groups of proteins) database, and KEGG (Kyoto Encyclopedia of Genes and Genomes) database. Among the 40,142 unigenes, 2,579 genes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dysphania schraderiana; De novo assembly; Transcriptome; Annotation; Terpenoid biosynthesis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300480 |
| |
|
| |
|
|
| Dubrulle, Guillaume; Picot, Adeline; Madec, Stephanie; Corre, Erwan; Pawtowski, Audrey; Baroncelli, Riccardo; Zivy, Michel; Balliau, Thierry; Le Floch, Gaétan; Pensec, Flora. |
| The fungal phytopathogen Colletotrichum lupini is responsible for lupin anthracnose, resulting in significant yield losses worldwide. The molecular mechanisms underlying this infectious process are yet to be elucidated. This study proposes to evaluate C. lupini gene expression and protein synthesis during lupin infection, using, respectively, an RNAseq-based transcriptomic approach and a mass spectrometry-based proteomic approach. Patterns of differentially-expressed genes in planta were evaluated from 24 to 84 hours post-inoculation, and compared to in vitro cultures. A total of 897 differentially-expressed genes were identified from C. lupini during interaction with white lupin, of which 520 genes were predicted to have a putative function, including... |
| Tipo: Text |
Palavras-chave: Anthracnose disease; Transcriptome; Proteome; Pathogenicity factors. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00656/76795/77961.pdf |
| |
|
|
| Shibao,Priscila Yumi Tanaka; Cologna,Camila Takeno; Morandi-Filho,Romualdo; Wiezel,Gisele Adriano; Fujimura,Patricia Tiemi; Ueira-Vieira,Carlos; Arantes,Eliane Candiani. |
| Abstract Background: Animal poisons and venoms are sources of biomolecules naturally selected. Rhinella schneideri toads are widespread in the whole Brazilian territory and they have poison glands and mucous gland. Recently, protein from toads’ secretion has gaining attention. Frog skin is widely known to present great number of host defense peptides and we hypothesize toads present them as well. In this study, we used a RNA-seq analysis from R. schneideri skin and biochemical tests with the gland secretion to unravel its protein molecules. Methods: Total RNA from the toad skin was extracted using TRizol reagent, sequenced in duplicate using Illumina Hiseq2500 in paired end analysis. The raw reads were trimmed and de novo assembled using Trinity. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RNA-seq; Rhinella schneideri; Toad secretion; Transcriptome; Illumina; Cutaneous secretion; Skin secretion; Toad protein. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100326 |
| |
|
| |
|
|
| Marie, Benjamin; Joubert, Caroline; Tayale, Alexandre; Zanella-cleon, Isabelle; Belliard, Corinne; Piquemal, David; Cochennec-laureau, Nathalie; Marin, Frederic; Gueguen, Yannick; Montagnani, Caroline. |
| Mollusca evolutionary success can be attributed partly to their efficiency to sustain and protect their soft body with an external biomineralized structure, the shell. Current knowledge of the protein set responsible for the formation of the shell microstructural polymorphism and unique properties remains largely patchy. In Pinctada margaritifera and Pinctada maxima, we identified 80 shell matrix proteins, among which 66 are entirely unique. This is the only description of the whole "biomineralization toolkit" of the matrices that, at least in part, is thought to regulate the formation of the prismatic and nacreous shell layers in the pearl oysters. We unambiguously demonstrate that prisms and nacre are assembled from very different protein repertoires.... |
| Tipo: Text |
Palavras-chave: Mantle; Mollusk shell matrix proteins; Proteome; Transcriptome; Evolution. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00118/22959/20922.pdf |
| |
|
|
| Panserat, S.; Hortopan, G. A.; Plagnes-juan, E.; Kolditz, C.; Lansard, M.; Skiba-cassy, S.; Esquerre, D.; Geurden, I.; Medale, Francoise; Kaushik, Sadasivam; Corraze, Genevieve. |
| Our objective was to analyse the hepatic transcriptomes of juvenile rainbow trout fed with a plant-based diet. We focused our analysis on the total replacement of fish meal (FM) and fish oil (FO) by a 100% plant-based diet (0% FM, 0% FO). We analysed the postprandial hepatic transcriptomes of rainbow trout fed the two diets 8 h after feeding. Six total hepatic RNAs from each dietary group were hybridised against a trout cDNA microarray (9K). After treatment of the data respecting the standard MIAME (Minimum Information About a Microarray Experiment) protocol, we found that 176 hepatic genes were differentially expressed between fish fed the two diets: 96 and 80 were over-expressed and under-expressed, respectively, in trout fed the plant-based diet. A... |
| Tipo: Text |
Palavras-chave: Rainbow trout; Transcriptome; Metabolism; Liver; Vegetable products; Marine ingredients. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6684.pdf |
| |
| Registros recuperados: 76 | |
|
|
|
