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| Registros recuperados: 43 | |
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| Sussarellu, Rossana; Huvet, Arnaud; Lapegue, Sylvie; Quillen, Virgile; Lelong, Christophe; Cornette, Florence; Jensen, Lasse Fast; Bierne, Nicolas; Boudry, Pierre. |
| Background Originating from Northeast Asia, the Pacific oyster Crassostrea gigas has been introduced into a large number of countries for aquaculture purpose. Following introduction, the Pacific oyster has turned into an invasive species in an increasing number of coastal areas, notably recently in Northern Europe. Methods To explore potential adaptation of reproductive traits in populations with different histories, we set up a common garden experiment based on the comparison of progenies from two populations of Pacific oyster sampled in France and Denmark and their hybrids. Sex ratio, condition index and microarray gene expression in gonads, were analyzed in each progeny (n = 60). Results A female-biased sex-ratio and a higher condition index were... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Adaptation; Invasiveness; Fertility; Sex-ratio; Transcriptome; Microarray. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00287/39829/38343.pdf |
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| Vieira,Luiz Gonzaga Esteves; Andrade,Alan Carvalho; Colombo,Carlos Augusto; Moraes,Ana Heloneida de Araújo; Metha,Ângela; Oliveira,Angélica Carvalho de; Labate,Carlos Alberto; Marino,Celso Luis; Monteiro-Vitorello,Claúdia de Barros; Monte,Damares de Castro; Giglioti,Éder; Kimura,Edna Teruko; Romano,Eduardo; Kuramae,Eiko Eurya; Lemos,Eliana Gertrudes Macedo; Almeida,Elionor Rita Pereira de; Jorge,Érika C.; Albuquerque,Érika V. S.; Silva,Felipe Rodrigues da; Vinecky,Felipe; Sawazaki,Haiko Enok; Dorry,Hamza Fahmi A.; Carrer,Helaine; Abreu,Ilka Nacif; Batista,João A. N.; Teixeira,João Batista; Kitajima,João Paulo; Xavier,Karem Guimarães; Lima,Liziane Maria de; Camargo,Luis Eduardo Aranha de; Pereira,Luiz Filipe Protasio; Coutinho,Luiz Lehmann; Lemos,Manoel Victor Franco; Romano,Marcelo Ribeiro; Machado,Marcos Antonio; Costa,Marcos Mota do Carmo; Sá,Maria Fátima Grossi de; Goldman,Maria Helena S.; Ferro,Maria Inês T.; Tinoco,Maria Laine Penha; Oliveira,Mariana C.; Van Sluys,Marie-Anne; Shimizu,Milton Massao; Maluf,Mirian Perez; Eira,Mirian Therezinha Souza da; Guerreiro Filho,Oliveiro; Arruda,Paulo; Mazzafera,Paulo; Mariani,Pilar Drummond Sampaio Correa; Oliveira,Regina L.B.C. de; Harakava,Ricardo; Balbao,Silvia Filippi; Tsai,Siu Mui; Mauro,Sonia Marli Zingaretti di; Santos,Suzana Neiva; Siqueira,Walter José; Costa,Gustavo Gilson Lacerda; Formighieri,Eduardo Fernandes; Carazzolle,Marcelo Falsarella; Pereira,Gonçalo Amarante Guimarães. |
| Coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. The genus Coffea belongs to the Rubiaceae family which includes other important plants. The genus contains about 100 species but commercial production is based only on two species, Coffea arabica and Coffea canephora that represent about 70 % and 30 % of the total coffee market, respectively. The Brazilian Coffee Genome Project was designed with the objective of making modern genomics resources available to the coffee scientific community, working on different aspects of the coffee production chain. We have single-pass sequenced a total of 214,964 randomly picked clones from 37 cDNA libraries of C. arabica, C. canephora and C. racemosa,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea; CDNA; EST; Transcriptome. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100008 |
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| Benzekri, Hicham; Armesto, Paula; Cousin, Xavier; Rovira, Mireia; Crespo, Diego; Alejandro Merlo, Manuel; Mazurais, David; Bautista, Rocio; Guerrero-fernandez, Dario; Fernandez-pozo, Noe; Ponce, Marian; Infante, Carlos; Zambonino, Jose Luis; Nidelet, Sabine; Gut, Marta; Rebordinos, Laureana; Planas, Josep V.; Begout, Marie-laure; Gonzalo Claros, M.; Manchado, Manuel. |
| Background Senegalese sole (Solea senegalensis) and common sole (S. solea) are two economically and evolutionary important flatfish species both in fisheries and aquaculture. Although some genomic resources and tools were recently described in these species, further sequencing efforts are required to establish a complete transcriptome, and to identify new molecular markers. Moreover, the comparative analysis of transcriptomes will be useful to understand flatfish evolution. Results A comprehensive characterization of the transcriptome for each species was carried out using a large set of Illumina data (more than 1,800 millions reads for each sole species) and 454 reads (more than 5 millions reads only in S. senegalensis), providing coverages ranging from... |
| Tipo: Text |
Palavras-chave: Soles; Transcriptome; Microarray; Orthology; Molecular markers; SoleaDB. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00238/34918/33626.pdf |
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| Fu,Suhong; Lei,Ming; Zhang,Yongqun; Deng,Zhaomin; Shi,Jing; Hao,Doudou. |
| Abstract Dysphania schraderiana is widely distributed in Lhasa (Tibet, China) and used as a traditional medicine. However, the lack of genetic information hinders the understanding of its physiological processes, such as the biosynthesis of secondary metabolites. Herein, we used Illumina Hiseq4000 platform to sequence the transcriptome of flower and leaf tissues from D. schraderiana for the first time. Totally, 40,142 unigenes were assembled from approximately 5.2 million clean reads. All unigenes underwent gene prediction and were subsequently annotated in a NR (NCBI non-redundant protein) database, COG (Clusters of Orthologous Groups of proteins) database, and KEGG (Kyoto Encyclopedia of Genes and Genomes) database. Among the 40,142 unigenes, 2,579 genes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dysphania schraderiana; De novo assembly; Transcriptome; Annotation; Terpenoid biosynthesis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300480 |
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| Dubrulle, Guillaume; Picot, Adeline; Madec, Stephanie; Corre, Erwan; Pawtowski, Audrey; Baroncelli, Riccardo; Zivy, Michel; Balliau, Thierry; Le Floch, Gaétan; Pensec, Flora. |
| The fungal phytopathogen Colletotrichum lupini is responsible for lupin anthracnose, resulting in significant yield losses worldwide. The molecular mechanisms underlying this infectious process are yet to be elucidated. This study proposes to evaluate C. lupini gene expression and protein synthesis during lupin infection, using, respectively, an RNAseq-based transcriptomic approach and a mass spectrometry-based proteomic approach. Patterns of differentially-expressed genes in planta were evaluated from 24 to 84 hours post-inoculation, and compared to in vitro cultures. A total of 897 differentially-expressed genes were identified from C. lupini during interaction with white lupin, of which 520 genes were predicted to have a putative function, including... |
| Tipo: Text |
Palavras-chave: Anthracnose disease; Transcriptome; Proteome; Pathogenicity factors. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00656/76795/77961.pdf |
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| Shibao,Priscila Yumi Tanaka; Cologna,Camila Takeno; Morandi-Filho,Romualdo; Wiezel,Gisele Adriano; Fujimura,Patricia Tiemi; Ueira-Vieira,Carlos; Arantes,Eliane Candiani. |
| Abstract Background: Animal poisons and venoms are sources of biomolecules naturally selected. Rhinella schneideri toads are widespread in the whole Brazilian territory and they have poison glands and mucous gland. Recently, protein from toads’ secretion has gaining attention. Frog skin is widely known to present great number of host defense peptides and we hypothesize toads present them as well. In this study, we used a RNA-seq analysis from R. schneideri skin and biochemical tests with the gland secretion to unravel its protein molecules. Methods: Total RNA from the toad skin was extracted using TRizol reagent, sequenced in duplicate using Illumina Hiseq2500 in paired end analysis. The raw reads were trimmed and de novo assembled using Trinity. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RNA-seq; Rhinella schneideri; Toad secretion; Transcriptome; Illumina; Cutaneous secretion; Skin secretion; Toad protein. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100326 |
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| Marie, Benjamin; Joubert, Caroline; Tayale, Alexandre; Zanella-cleon, Isabelle; Belliard, Corinne; Piquemal, David; Cochennec-laureau, Nathalie; Marin, Frederic; Gueguen, Yannick; Montagnani, Caroline. |
| Mollusca evolutionary success can be attributed partly to their efficiency to sustain and protect their soft body with an external biomineralized structure, the shell. Current knowledge of the protein set responsible for the formation of the shell microstructural polymorphism and unique properties remains largely patchy. In Pinctada margaritifera and Pinctada maxima, we identified 80 shell matrix proteins, among which 66 are entirely unique. This is the only description of the whole "biomineralization toolkit" of the matrices that, at least in part, is thought to regulate the formation of the prismatic and nacreous shell layers in the pearl oysters. We unambiguously demonstrate that prisms and nacre are assembled from very different protein repertoires.... |
| Tipo: Text |
Palavras-chave: Mantle; Mollusk shell matrix proteins; Proteome; Transcriptome; Evolution. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00118/22959/20922.pdf |
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| Panserat, S.; Hortopan, G. A.; Plagnes-juan, E.; Kolditz, C.; Lansard, M.; Skiba-cassy, S.; Esquerre, D.; Geurden, I.; Medale, Francoise; Kaushik, Sadasivam; Corraze, Genevieve. |
| Our objective was to analyse the hepatic transcriptomes of juvenile rainbow trout fed with a plant-based diet. We focused our analysis on the total replacement of fish meal (FM) and fish oil (FO) by a 100% plant-based diet (0% FM, 0% FO). We analysed the postprandial hepatic transcriptomes of rainbow trout fed the two diets 8 h after feeding. Six total hepatic RNAs from each dietary group were hybridised against a trout cDNA microarray (9K). After treatment of the data respecting the standard MIAME (Minimum Information About a Microarray Experiment) protocol, we found that 176 hepatic genes were differentially expressed between fish fed the two diets: 96 and 80 were over-expressed and under-expressed, respectively, in trout fed the plant-based diet. A... |
| Tipo: Text |
Palavras-chave: Rainbow trout; Transcriptome; Metabolism; Liver; Vegetable products; Marine ingredients. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6684.pdf |
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| Riviere, Guillaume; Klopp, Christophe; Ibouniyamine, Nabihoudine; Huvet, Arnaud; Boudry, Pierre; Favrel, Pascal. |
| Background: The Pacific oyster, Crassostrea gigas, is one of the most important aquaculture shellfish resources worldwide. Important efforts have been undertaken towards a better knowledge of its genome and transcriptome, which makes now C. gigas becoming a model organism among lophotrochozoans, the under-described sister clade of ecdysozoans within protostomes. These massive sequencing efforts offer the opportunity to assemble gene expression data and make such resource accessible and exploitable for the scientific community. Therefore, we undertook this assembly into an up-to-date publicly available transcriptome database: the GigaTON (Gigas TranscriptOme pipeliNe) database. Description: We assembled 2204 million sequences obtained from 114 publicly... |
| Tipo: Text |
Palavras-chave: Oyster; Transcriptome; Development; Stress; Tissues; NGS. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00302/41321/40520.pdf |
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| Prieto, Daniel; Markaide, Pablo; Urrutxurtu, Iñaki; Navarro, Enrique; Artigaud, Sebastien; Fleury, Elodie; Ibarrola, Irrintzi; Urrutia, Miren Bego. |
| The molecular basis underlying the mechanisms at the origin of growth variation in bivalves is still poorly understood, although several genes have been described as upregulated in fast-growing individuals. In the present study, we reared mussel spat of the species Mytilus galloprovincialis under diets below the pseudofaeces threshold (BP) and above the pseudofaeces threshold (AP). After 3 months, F and S mussels from each condition were selected to obtain 4 experimental groups: FBP, SBP, FAP and SAP. We hypothesized that the nurturing conditions during the growing period would modify the molecular basis of their growth rate differences. To decipher the molecular mechanisms underlying the growth variation, the gill transcriptomes for the four mussel groups... |
| Tipo: Text |
Palavras-chave: Fast-growing; Mussel; Gill; Transcriptome. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00503/61490/65293.pdf |
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| Teaniniuraitemoana, Vaihiti; Huvet, Arnaud; Levy, Peva; Klopp, Christophe; Lhuillier, Emeline; Gaertner-mazouni, Nabila; Gueguen, Yannick; Le Moullac, Gilles. |
| Background Black pearl farming is based on culture of the blacklip pearl oyster Pinctada margaritifera (Mollusca, lophotrochozoa), a protandrous hermaphrodite species. At first maturation, all individuals are males. The female sex appears progressively from two years old, which represents a limitation for broodstock conditioning for aquaculture production. In marine mollusks displaying hermaphroditic features, data on sexual determinism and differentiation, including the molecular sex determining cascade, are scarce. To increase genomic resources and identify the molecular mechanisms whereby gene expression may act in the sexual dimorphism of P. margaritifera, we performed gonad transcriptome analysis. Results The gonad transcriptome of P. margaritifera... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; Gametogenesis; Transcriptome; Differential expression; Sex determinism. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00201/31194/29593.pdf |
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| Vogel,R.F.; Pavlovic,M.; Hörmann,S.; Ehrmann,M.A.. |
| Lactobacillus sanfranciscensis is a Gram-positive lactic acid bacterium used in food biotechnology. It is necessary to investigate many aspects of a model organism to elucidate mechanisms of stress response, to facilitate preparation, application and performance in food fermentation, to understand mechanisms of inactivation, and to identify novel tools for high pressure biotechnology. To investigate the mechanisms of the complex bacterial response to high pressure we have analyzed changes in the proteome and transcriptome by 2-D electrophoresis, and by microarrays and real time PCR, respectively. More than 16 proteins were found to be differentially expressed upon high pressure stress and were compared to those sensitive to other stresses. Except for one... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: High pressure; Lactobacillus sanfranciscensis; Proteome; Transcriptome; Stress response. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800013 |
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| Amaral,Alexandre Morais do; Saito,Daniel; Formighieri,Eduardo Fernandes; Rabello,Edenilson; Souza,Adriane N. de; Silva-Stenico,Maria Estela; Tsai,Siu Mui. |
| Pleiotropic drug resistance (PDR) proteins, a subfamily of the ATP-binding cassette (ABC) transporters, have been recently shown to play a role in plant defense against biotic and abiotic stresses. However, nothing is known about their expression in citrus. To investigate the occurrence of PDR homologues in citrus species, we have surveyed EST sequences from different tissues and conditions of the Citrus Expressed Sequence Tags (CitEST) database, through sequence similarity search analyses and inspections for characteristic PDR domains. Multiple sequence alignments, prediction of transmembrane topology and phylogenetic analysis of PDR-like proteins were additionally performed. This study allowed the identification of nine putative proteins showing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ABC transporters; CitEST; Expressed sequence tag; Transcriptome; Stress. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500014 |
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| Mazurais, David; Ferraresso, Serena; Gatta, Pier Paolo; Desbruyeres, Elisabeth; Severe, Armelle; Corporeau, Charlotte; Claireaux, Guy; Bargelloni, Luca; Zambonino, Jose-luis. |
| Coastal systems could be affected by hypoxic events brought about by global change. These areas are essential nursery habitats for several fish species including the common sole (Solea solea L.). Tolerance of fish to hypoxia depends on species and also on their physiological condition and nutritional status. Indeed, high dietary lipid content has been recently shown to negatively impact the resistance of sole to a severe hypoxic challenge. In order to study the molecular mechanisms involved in the early response to hypoxic stress, the present work examined the hepatic transcriptome in common sole fed diets with low and high lipid content, exposed to severe hypoxia. The activity of AMP-activated protein kinase (AMPK) was also investigated through the... |
| Tipo: Text |
Palavras-chave: Fish; Hypoxia; Nutrition; Liver; Transcriptome; AMPK. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00169/28062/26474.pdf |
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| Registros recuperados: 43 | |
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