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A guideline to improve qualitative social science publishing in ecology and conservation journals Ecology and Society
Moon, Katie; Institute for Applied Ecology, University of Canberra; School of Business, University of New South Wales; katieamoon@gmail.com; Brewer, Tom D.; Northern Institute, Charles Darwin University, Darwin, Northern Territory, Australia; Australian Institute of Marine Science, Arafura Timor Research Facility, Brinkin, Northern Territory, Australia; tomdbrewer@gmail.com; Adams, Vanessa M.; University of Queensland, School of Biological Sciences, Brisbane, Queensland, Australia; v.adams@uq.edu.au; Blackman, Deborah A.; School of Business, University of New South Wales; d.blackman@adfa.edu.au.
A rise in qualitative social science manuscripts published in ecology and conservation journals speaks to the growing awareness of the importance of the human dimension in maintaining and improving Earth’s ecosystems. Given the rise in the quantity of qualitative social science research published in ecology and conservation journals, it is worthwhile quantifying the extent to which this research is meeting established criteria for research design, conduct, and interpretation. Through a comprehensive review of this literature, we aimed to gather and assess data on the nature and extent of information presented on research design published qualitative research articles, which could be used to judge research quality. Our review was based on 146...
Tipo: Peer-Reviewed Insight Palavras-chave: Case study; Confirmability; Credibility; Dependability; Methods; Transferability.
Ano: 2016
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Aspectos bioecológicos e genético-comportamentais envolvidos na conservação da abelha Jandaíra, Melipona subnitida Ducke (Apidae, Meliponini), e o uso de ferramentas moleculares nos estudos de diversidade. Repositório Alice
SILVA, G. R. da; PEREIRA, F. de M.; SOUZA, B. de A.; LOPES, M. T. do R.; CAMPELO, J. E. G.; DINIZ, F. M..
A abelha sem ferrão Melipona subnitida atualmente está presente em quase toda a região nordeste, em função da boa adaptabilidade ao semiárido nordestino e do potencial econômico-ecológico proporcionado pela produção de mel e pela polinização de cultivos em condições de confinamento. Apesar disso, é uma espécie ameaçada devido a processos de degradação ambiental, dentre os quais estão o desmatamento, o uso indiscriminado de agrotóxicos e o extrativismo. Tais interferências tendem a isolar as populações de Jandaíra, provocando uma queda na variabilidade genética e, consequentemente, uma redução na capacidade adaptativa da espécie. Porém, técnicas de biologia molecular estão sendo implementadas, possibilitando que populações desse tipo sejam avaliadas quanto...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Abelha nativa; Microssatélite; Transferibilidade; Diversidade genética; Conservação; Native bees; Microsatellites; Transferability; Genetic diversity; Conservation.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1017704
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Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae) Genet. Mol. Biol.
Lopes,Denilce Meneses; Campos,Lúcio Antônio de Oliveira; Salomão,Tânia Maria Fernandes; Tavares,Mara Garcia.
Due to their high degree of polymorphism, microsatellites are considered useful tools for studying population genetics. Nevertheless, studies of genetic diversity in stingless bees by means of these primers have revealed a low level of polymorphism, possibly the consequence of the heterologous primers used, since in most cases these were not specifically designed for the species under consideration. Herein we compared the number of polymorphic loci and alleles per locus, as well as observed heterozygosity in Melipona rufiventris and M. mondury populations, using specific and heterologous primers. The use of specific primers placed in evidence the greater frequency of polymorphic loci and alleles per locus, besides an expressive increase in observed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Melipona; Stingless bees; Microsatellite; Transferability.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200030
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Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae) Genet. Mol. Biol.
Bombonato,Juliana Rodrigues; Bonatelli,Isabel Aparecida Silva; Silva,Gislaine Angélica Rodrigues; Moraes,Evandro Marsola; Zappi,Daniela Cristina; Taylor,Nigel P.; Franco,Fernando Faria.
Abstract The study of transferability of simple sequence repeats (SSR) among closely related species is a well-known strategy in population genetics, however transferability among distinct genera is less common. We tested cross-genera SSR amplification in the family Cactaceae using a total of 20 heterologous primers previously developed for the genera Ariocarpus, Echinocactus, Polaskia and Pilosocereus, in four taxa of the genus Cereus: C. fernambucensis subsp. fernambucensis, C. fernambucensis subsp. sericifer, C. jamacaru and C. insularis. Nine microsatellite loci were amplified in Cereus resulting in 35.2% of success in transferability, which is higher than the average rate of 10% reported in the literature for cross-genera transferability in eudicots....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cactaceae; Cereus; Cross-genera; SSR markers; Transferability.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100087
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Cross-species amplification of microsatellite loci developed for Passiflora edulis Sims. in related Passiflora Species BABT
Oliveira,Gilmara Alvarenga Fachardo; Pádua,Juliano Gomes; Costa,Juliana Leles; Jesus,Onildo Nunes de; Carvalho,Fabiana Moraes de; Oliveira,Eder Jorge de.
The aim of this study was to evaluate the selected 41 SSR markers developed for yellow passion fruit (Passiflora edulis f. flavicarpa Sims.) for their transferability to 11 different Passiflora species. Twenty-one SSR were successfully amplified in 10 wild species of passion fruit producing 101 bands. All the markers were amplifiable for at least one species. The mean transferability was 68,8%, ranging from 15,4% (primer PE11) to 100 % (PE13, PE18, PE37, PE41 and PE88). Transferability was higher for the species from the Passiflora subgenus than for those from the Decaloba and Dysosmia subgenus. The results indicated a high level of nucleotide sequence conservation of the primer regions in the species evaluated, and consequently, they could potentially be...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transferability; SSR; Passion fruit.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500009
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Development, Validation and Characterization of Genic Microsatellite Markers in Urochloa Species. Repositório Alice
SOUZA, J. S.; CHIARI, L.; RESENDE, R. M. S.; VILELA, M. de M.; SALGADO, L. R..
Urochloa (syn. Brachiaria ) cultivars represent 85% of tropical pastures occupying 114 million hectares of cultivated grasslands in Brazil. Despite the commercial importance of the Urochloa species, low molecular information is available and is far from saturating the genome. Investigating and obtaining more markers associated to characteristics of difficult and late expression can benefit in accelerating breeding programs of more important species. Aiming to increase the number of molecular markers, genic microsatellite markers were obtained from transcriptome of U. decumbens and analyzed for their cross-amplification to U. brizantha , U. humidicola and U. ruziziensis . Genic microsatellite markers were isolated from a transcriptome obtained of U....
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Signalgrass; Simple Sequence Repeat; Transferability; Brachiaria.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1101685
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Genetic characterization of 12 heterologous microsatellite markers for the giant tropical tree Cariniana legalis (Lecythidaceae) Genet. Mol. Biol.
Guidugli,Marcela Corbo; Accoroni,Klaus Alvaro Guerrieri; Mestriner,Moacyr Antonio; Contel,Eucleia Primo Betioli; Martinez,Carlos Alberto; Alzate-Marin,Ana Lilia.
Twelve microsatellite loci previously developed in the tropical tree Cariniana estrellensis were genetically characterized in Cariniana legalis. Polymorphisms were assessed in 28 C. legalis individuals found between the Pardo and Mogi-Guaçu River basins in the state of São Paulo, Brazil. Of the 12 loci, 10 were polymorphic and exhibited Mendelian inheritance. The allelic richness at each locus ranged from 2-11, with an average of 7 alleles per locus, and the expected heterozygosity ranged from 0.07-0.88. These loci showed a high probability of paternity exclusion. The characteristics of these heterologous microsatellite markers indicate that they are suitable tools for investigating questions concerning population genetics in C. legalis.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Jequitibá rosa; Population genetics; SSR markers; Transferability.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100022
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Genetic diversity analysis among pigeonpea genotypes adapted to South American regions based on microsatellite markers Scientia Agricola
Sousa,Adna Cristina Barbosa de; Godoy,Rodolfo; Sforça,Danilo Augusto; Campos,Tatiana de; Zucchi,Maria Imaculada; Jank,Liana; Souza,Anete Pereira de.
The pigeonpea [Cajanus cajan (L) Millspaugh] is one of the most important perennial legume crops utilized in the food, fodder, soil conservation, crop-livestock integrated systems, reclaiming of degraded pastures and symbiotic nitrogen fixation. Microsatellite markers were used to estimate the genetic diversity of 77 pigeonpea genotypes selected from the germplasm collections at Embrapa Cattle-Southeast and, to evaluate their transferability to Phaseolus vulgaris and Vigna unguiculata species. The number of alleles per locus ranged from 2 to12, with an average of 5.1 alleles. The PIC values ranged from 0.11 to 0.80 (average 0.49) and the D values from 0.23 to 0.91 (average 0.58). The averages of observed and expected heterozygosity were 0.25 and 0.47,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Legumes; Transferability; Microsatellite; Germplasm.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400007
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Genetic diversity of Brazilian triticales evaluated with genomic wheat microsatellites PAB
Costa,Cibele Tesser da; Albuquerque,Ana Christina Sagebin; Nascimento Junior,Alfredo do; Marcelino,Francismar Correa; Pereira,Jorge Fernando.
The objective of this work was to determine the genetic variability available for triticale (X Triticosecale Wittmack) crop improvement in Brazil. Forty-two wheat genomic microsatellites were used to estimate the molecular diversity of 54 genotypes, which constitute the base of one of the major triticale breeding programs in the country. Average heterozygosity was 0.06 and average and effective number of alleles per locus were 2.13 and 1.61, respectively, with average allelic frequency of 0.34. The set of genomic wheat microsatellites used clustered the genotypes into seven groups, even when the germplasm was originated primarily from only two triticale breeding programs, a fact reflected on the average polymorphic information content value estimated for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: X Triticosecale; Polymorphism information content; Transferability; Heterozygosity; Number of alleles; Frequency of alleles.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007001100009
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Genetic diversity of Brazilian triticales evaluated with genomic wheat microsatellites. Repositório Alice
COSTA, C. T. da; ALBUQUERQUE, A. C. S.; NASCIMENTO JUNIOR, A. do; MARCELINO, F. C.; PEREIRA, J. F..
2007
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: X Triticosecale; Índice de polimorfismo; Transferabilidade; Heterozigosidade; Número efetivo de alelos; Freqüência alélica; Polymorphism information content; Transferability; Heterozygosity; Number of alleles; Frequency of alleles.
Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/handle/doc/822347
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Genetic diversity of Brazilian triticales evaluated with genomic wheat microsatellites Repositório Alice
COSTA, C.T. da; ALBUQUERQUE, A.C.S.; NASCIMENTO JUNIOR, A. do; MARCELINO, F.C.; PEREIRA, J.F..
The objective of this work was to determine the genetic variability available for triticale (X Triticosecale Wittmack) crop improvement in Brazil. Forty-two wheat genomic microsatellites were used to estimate the molecular diversity of 54 genotypes, which constitute the base of one of the major triticale breeding programs in the country. Average heterozygosity was 0.06 and average and effective number of alleles per locus were 2.13 and 1.61, respectively, with average allelic frequency of 0.34. The set of genomic wheat microsatellites used clustered the genotypes into seven groups, even when the germplasm was originated primarily from only two triticale breeding programs, a fact reflected on the average polymorphic information content value estimated for...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: X Triticosecale; Polymorphism information content; Transferability; Heterozygosity; Number of alleles; Frequency of alleles; Índice de polimorfismo; Transferabilidade; Heterozigosidade; Número efetivo de alelos; Freqüência alélica.
Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/handle/doc/122827
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Genetic diversity of cultivated accessions and wild species of rubber tree using EST‑SSR markers PAB
Perseguini,Juliana Morini Küpper Cardoso; Romão,Lineu Roberto de Castro; Briñez,Boris; Scaloppi Junior,Erivaldo José; Gonçalves,Paulo de Souza; Benchimol,Luciana Lasry.
The objective of this work was to evaluate the efficiency of EST‑SSR markers in the assessment of the genetic diversity of rubber tree genotypes (Hevea brasiliensis) and to verify the transferability of these markers for wild species of Hevea. Forty‑five rubber tree accessions from the Instituto Agronômico (Campinas, SP, Brazil) and six wild species were used. Information provided by modified Roger's genetic distance were used to analyze EST‑SSR data. UPGMA clustering divided the samples into two major groups with high genetic differentiation, while the software Structure distributed the 51 clones into eight groups. A parallel could be established between both clustering analyses. The 30 polymorphic EST‑SSRs showed from two to ten alleles and were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fingerprinting analysis; Genetic structure; Functional molecular markers; Polymorphism information content; Transferability.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000800008
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Microsatellite loci for Urochloa decumbens (Stapf) R. D. Webster and cross-amplification in other Urochloa species. Repositório Alice
FERREIRA, R. C. U.; CANÇADO, L. J.; VALLE, C. B. do; CHIARI, L.; SOUZA, A. P. de..
Background: Forage grasses of the African genus Urochloa (syn. Brachiaria) are the basis of Brazilian beef production, and there is a strong demand for high quality, productive and adapted forage plants. Among the approximately 100 species of the genus Urochloa, Urochloa decumbens is one of the most important tropical forage grasses used for pastures due to several of its agronomic attributes. However, the level of understanding of these attributes and the tools with which to control them at the genetic level are limited, mainly due to the apomixis and ploidy level of this species. In this context, the present study aimed to identify and characterize molecular microsatellite markers of U. decumbens and to evaluate their cross-amplification in other...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Enriched library; Transferability; Forage; Urochloa brizantha; Microsatellite repeats.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1062128
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Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis Genet. Mol. Biol.
Bravo,Juliana Pereira; Hoshino,Andrea Akemi; Angelici,Carla Maria Lara C.D.; Lopes,Catalina Romero; Gimenes,Marcos Aparecido.
The Arachis section is the most important of the nine sections of the genus Arachis because it includes the cultivated peanut, Arachis hypogaea. The genetic improvement of A. hypogaea using wild relatives is at an early stage of development in spite of their potential as sources of genes, including those for disease and pests resistance, that are not found in the A. hypogaea primary gene pool. Section Arachis species germplasm has been collected and maintained in gene banks and its use and effective conservation depends on our knowledge of the genetic variability contained in this material. Microsatellites are routinely used for the analysis of genetic variability because they are highly polymorphic and codominant. The objective of this study was to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic diversity; Germplasm; Peanut; Transferability; Wild species.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300021
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Transference of microsatellite markers from Eucalyptus spp to Acca sellowiana and the successful use of this technique in genetic characterization Genet. Mol. Biol.
Santos,Karine Louise dos; Welter,Leocir José; Dantas,Adriana Cibele de Mesquita; Guerra,Miguel Pedro; Ducroquet,Jean Pierre Henri Joseph; Nodari,Rubens Onofre.
The pineapple guava (Acca sellowiana), known in portuguese as the goiabeira-serrana or "Feijoa", is a native fruit tree from southern Brazil and northern Uruguay that has commercial potential due to the quality and unique flavor of its fruits. Knowledge of genetic variability is an important tool in various steps of a breeding program, which can be facilitated by the use of molecular markers. The conservation of repeated sequences among related species permits the transferability of microsatellite markers from Eucalyptus spp. to A. sellowiana for testing. We used primers developed for Eucalyptus to characterize A. sellowiana accessions. Out of 404 primers tested, 180 amplified visible products and 38 were polymorphic. A total of 48 alleles were detected...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Feijoa sellowiana; Genetic diversity; Goiabeira-serrana; Pineapple-guava; Transferability.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100014
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