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A systemic framework for context-based decision making in natural resource management: reflections on an integrative assessment of water and livelihood security outcomes following policy reform in South Africa Ecology and Society
Pollard, Sharon; The Association for Water and Rural Development; sharon@award.org.za; Biggs, Harry; SANParks; Harry.Biggs@sanparks.org; Du Toit, Derick R; The Association for Water and Rural Development; derick@award.org.za.
We aimed to contribute to the field of natural resource management (NRM) by introducing an alternative systemic context-based framework for planning, research, and decision making, which we expressed practically in the development of a decision-making “tool” or method. This holistic framework was developed in the process of studying a specific catchment area, i.e., the Sand River Catchment, but we have proposed that it can be generalized to studying the complexities of other catchment areas. Using the lens of systemic resilience to think about dynamic and complex environments differently, we have reflected on the development of a systemic framework for understanding water and livelihood security under transformation in postapartheid...
Tipo: Peer-Reviewed Reports Palavras-chave: Complexity; Decision making; Dynamic; Governance; IWRM; Livelihood security; Resilience; SES; Social-ecological systems; Transdisciplinarity; Transformation.
Ano: 2014
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Adaptacao de um protocolo para transformacao genetica de batata (Solanum tuberosum L.) cultivar Bintje visando introducao de resistencia a PVY. Repositório Alice
PASCOAL, A.V.; GAMA, M.I.C.S.; BRAVO-ALMONOACID, F.; MENTABERRY, A.; ROMANO, E.; DUSI, D.; MELLO, P.; MONTE-NESHICH, D..
1996
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Solanum tuberosum; Protocolo; Genetica; PVY; Transformacao; Protocol; Genetic; Transformation.
Ano: 1996 URL: http://www.alice.cnptia.embrapa.br/handle/doc/171514
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Adaptive Capacity and Traps Ecology and Society
Carpenter, Stephen R; University of Wisconsin-Madison; srcarpen@wisc.edu; Brock, William A.; University of Wisconsin-Madison; WBrock@ssc.wisc.edu.
Adaptive capacity is the ability of a living system, such as a social–ecological system, to adjust responses to changing internal demands and external drivers. Although adaptive capacity is a frequent topic of study in the resilience literature, there are few formal models. This paper introduces such a model and uses it to explore adaptive capacity by contrast with the opposite condition, or traps. In a social–ecological rigidity trap, strong self-reinforcing controls prevent the flexibility needed for adaptation. In the model, too much control erodes adaptive capacity and thereby increases the risk of catastrophic breakdown. In a social–ecological poverty trap, loose connections prevent the mobilization of ideas and resources...
Tipo: Peer-Reviewed Insight Palavras-chave: Adaptation; Allostasis; Model; Poverty trap; Resilience; Rigidity trap; Transformation.
Ano: 2008
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Agrobacterium-mediated transformation of Guignardia citricarpa: an efficient tool to gene transfer and random mutagenesis. Repositório Alice
RODRIGUES, M. B. C.; FAVARO, L. C. de L.; PALUU, A. P. de. S.; FERREIRA, A.; SEBASTIANES, F. de. S.; RODRIGUES, M. J. C.; SPOSITO, M. B.; ARAUJO, W. L. de.; PIZZIRANI-KLEINER, A. A..
Guignardia citricarpa is the causal agent of Citrus Black Spot (CBS), an important disease in Citriculture. Due to the expressive value of this activity worldwide, especially in Brazil, understanding more about the functioning of this fungus is of utmost relevance, making possible the elucidation of its infection mechanisms, and providing tools to control CBS. This work describes for the first time an efficient and successful methodology for genetic transformation of G. citricarpa mycelia, which generated transformants expressing the gene encoding for the gfp (green fluorescent protein) and also their interaction with citrus plant. Mycelia of G. citricarpa were transformed via Agrobacterium tumefaciens, which carried the plasmid pFAT-gfp, contains the...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Agrobacterium-mediated; Transformation; Citrus Black Spot; Genetic transformation; Guignardia citricarpa; Plant e pathogen interaction.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/970991
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Agrobacterium-mediated transformation of sorghum. Repositório Alice
CARVALHO, C.H.S.; ZEHR, U.B.; GUNARATNA, N.; ANDERSON, J.; KONONOWICZ, H.; HODGES, T.K.; AXTELL, J.D..
2000
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Sorgo; Transformacao; Genetica; Cultura de tecidos; Sorghum bicolor; Agrobacterium; Genetic; Transformation; Tissue culture.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/484026
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Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency Genet. Mol. Biol.
Carvalho,Carlos Henrique S.; Zehr,Usha B.; Gunaratna,Nilupa; Anderson,Joseph; Kononowicz,Halina H.; Hodges,Thomas K.; Axtell,John D..
The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agrobacterium tumefaciens; GUS; Transformation; Sorghum.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200022
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An improved system for competent cell preparation and high efficiency plasmid transformation using different Escherichia coli strains Electron. J. Biotechnol.
Tu,Zhiming; He,Guangyuan; Li,Kexiu X.; Chen,Mingjie J.; Chang,Junli; Chen,Ling; Yao,Qing; Liu,Dongping P.; Ye,Huan; Shi,Jiantao; Wu,Xuqian .
This paper describes an efficient bacterial transformation system that was established for the preparation of competent cells, plasmid preparation, and for the storage in bacterial stocks in our laboratory. Using this method, a number of different plasmids have been amplified for further experiments. Competent cells for bacterial transformation were prepared by the calcium chloride method with an optimum concentration of 75 mM. Three different strains of Escherichia coli that were tested are DH5α, TG1 and XL1 blue, and the most efficient strain being XL1 blue. The optimal optical density (OD600) range for competent cell preparation varied for each of the strains investigated, and for XL1 blue it was 0.15-0.45; for TG1 it was 0.2-0.5; and for...
Tipo: Journal article Palavras-chave: Competent cells; E. coli; Plasmid; Storage; Transformation.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100014
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Analysis and sequencing of h6hmRNA, last enzyme in the tropane alkaloids pathway from anthers and hairy root cultures of Brugmansia candida (Solanaceae) Electron. J. Biotechnol.
Cardillo,Alejandra B.; Giulietti,Ana M.; Marconi,Patricia L..
Brugmansia candida (Solanaceae) is a native tree distributed across South-American and produces the pharmacologically- important group of tropane alkaloids including scopolamine. This biocompound is synthesised from hyoscyamine by action of Hyoscyamine 6-β hydroxylase (H6H, EC 1.14.11.11) at the end of the tropane alkaloid pathway. Here are reported the tissue and organ-specific expression of h6hmRNA by RT-PCR analyses and the isolation, cloning and sequencing of the cDNA obtained from B. candida anthers and hairy root transformed cultures. Bioinformatic analysis of the nucleotide sequence revealed an uninterrupted ORF of 1038 bp and the predicted aminoacid sequence could be 344 aminoacid long. A database search showed that this sequence has high...
Tipo: Journal article Palavras-chave: H6H; Hairy roots; Hyoscyamine; Scopolamine; Sequence analysis; Transformation.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300004
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Avaliacoes da infeccao de explantes de feijoeiro por Agrobacterium tumefaciens por meio de microscopia. Infoteca-e
ANDRADE, S. R. M.; GLORIA, B. A.; LABATE, M. T. V.; GOMES, D. G.; LABATE, C. A..
Phaseolus vulgaris L. e considerada recalcitrante a transformacao por Agrobacterium tumefaciens. Contudo, alteracoes no meio de co-cultivo, utilizacao de linhagens hipervirulentas de Agrobacterium e de vetores binarios contendo genes vir demostraram que o feijoeiro e susceptivel a essa bacteria. O objetivo do presente trabalho foi estudar o efeito da sonificacao nos tecidos vegetais de feijoeiro, bem como a penetracao da Agrobacterium nas camadas subepidermicas do tecido vegetal, usando a metodologia SAAT ("Sonification-Assisted Agrobacterium-mediated Transformation"). A variedade de feijoeiro utilizada foi a Olathe Pinto, a linhagem de A. tumefaciens foi LBA4404:pTOK.Os embrioes de feijao foram pre-tratados po 14 dias em meio de multibrotacao e, entao...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Cerrado; Feijao; Phaseolus vulgaris; Genetica vegetal; Infeccao; Bacteria; Transformation; SAAT; Common bean; Plant genetics; Biotecnologia: transgenico.
Ano: 2001 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/567067
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Banana protoplasts: culture and its applications. Repositório Alice
MATSUMOTO, K.; MONTE, D. de C.; TEIXEIRA, J. B.; HAICOUR, R.; DAVEY, M. R..
bitstream/item/183137/1/TFSB-4SI132-38o.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Nurse culture; Transformation; Musa; Somatic hybridization.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/880183
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Biodiversity governance and social-ecological system dynamics: transformation in the Australian Alps Ecology and Society
Lockwood, Michael; Geography and Environmental Studies, School of Land & Food, University of Tasmania; Michael.Lockwood@utas.edu.au; Mitchell, Michael; Geography and Environmental Studies, School of Land & Food, University of Tasmania; Michael.Mitchell@utas.edu.au; Moore, Susan A.; School of Veterinary and Life Sciences, Murdoch University ; S.Moore@murdoch.edu.au; Clement, Sarah; School of Veterinary and Life Sciences, Murdoch University ; S.Clement@murdoch.edu.au.
Biodiversity conservation continues to be a challenging task for societies worldwide. We undertook a resilience assessment to address the following question: What are the ramifications of social-ecological system dynamics for biodiversity governance of a nationally significant landscape? Resilience assessment offers promise for guiding response strategies, potentially enabling consideration of ecological, social, economic, and governance influences on biodiversity-related activities. Most resilience assessments have, however, struggled to effectively incorporate governance influences. We applied a modified version of the Resilience Alliance workbook approach to explicitly address governance influences at each stage of an assessment of internationally...
Tipo: Peer-Reviewed Reports Palavras-chave: Adaptive cycle; Biodiversity; Climate change; Governance; Resilience assessment; Transformation.
Ano: 2014
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Building resilient pathways to transformation when “no one is in charge”: insights from Australia's Murray-Darling Basin Ecology and Society
Abel, Nick; Land and Water, Commonwealth Scientific and Industrial Research Organisation ; nick.abel@csiro.au; Wise, Russell M.; Land and Water, Commonwealth Scientific and Industrial Research Organisation; russell.wise@csiro.au; Colloff, Matthew J.; Land and Water, Commonwealth Scientific and Industrial Research Organisation; Matt.Colloff@csiro.au; Walker, Brian H.; Land and Water, Commonwealth Scientific and Industrial Research Organisation; brian.walker@csiro.au; Butler, James R. A.; Land and Water, Commonwealth Scientific and Industrial Research Organisation; james.butler@csiro.au; Ryan, Paul; Australian Resilience Centre; paulryan@internode.on.net; Norman, Chris; Goulburn Broken Catchment Management Authority; chrisn@gbcma.vic.gov.au; Langston, Art; Land and Water, Commonwealth Scientific and Industrial Research Organisation; art.langston@csiro.au; Anderies, John M.; Arizona State University; m.anderies@asu.edu; Gorddard, Russell; Land and Water, Commonwealth Scientific and Industrial Research Organisation; Russell.Gorddard@csiro.au; Dunlop, Michael; Land and Water, Commonwealth Scientific and Industrial Research Organisation; michael.dunlop@csiro.au; O'Connell, Deborah; Land and Water, Commonwealth Scientific and Industrial Research Organisation; deborah.o'connell@csiro.au.
Climate change and its interactions with complex socioeconomic dynamics dictate the need for decision makers to move from incremental adaptation toward transformation as societies try to cope with unprecedented and uncertain change. Developing pathways toward transformation is especially difficult in regions with multiple contested resource uses and rights, with diverse decision makers and rules, and where high uncertainty is generated by differences in stakeholders’ values, understanding of climate change, and ways of adapting. Such a region is the Murray-Darling Basin, Australia, from which we provide insights for developing a process to address these constraints. We present criteria for sequencing actions along adaptation pathways: feasibility...
Tipo: Peer-Reviewed Insight Palavras-chave: Adaptation pathways; Climate change; Collective action; Domain shift; Equity; Irrigation; Resilience; Social conflict; Transformation; Wetlands.
Ano: 2016
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Comparative study of wild and transformed salt tolerant bacterial strains on Triticum aestivum growth under salt stress BJM
Afrasayab,Shazia; Faisal,Muhammad; Hasnain,Shahida.
Eleven salt tolerant bacteria isolated from different sources (soil, plants) and their transformed strains were used to study their influence on Triticum aestivum var. Inqlab-91 growth under salt (100 mM NaCl) stress. Salt stress caused reduction in germination (19.4%), seedling growth (46%) and fresh weight (39%) in non-inoculated plants. In general, both wild and transformed strains stimulated germination, seedling growth and fresh weight in salt free and salt stressed conditions. At 100 mM NaCl, Staphylococcus xylosus ST-1 caused 25% increments in seedling length over respective control. Soluble protein content significantly enhanced (49%) under salt stress as compared to salt free control. At 100 mM NaCl parental strain PT-5 resulted about 32%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Plasmid; Rhizobacteria; Salinity; Auxin; Transformation.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400013
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Continuity and Change in Social-ecological Systems: the Role of Institutional Resilience Ecology and Society
Pahl-Wostl, Claudia; Institute for Environmental Systems Research, University of Osnabrueck; pahl@usf.uni-osnabrueck.de.
In recent years recurring political, economic, and environmental crises require questioning and re-evaluating dominant pathways of human development. However, political and economic frameworks seem to encompass deeply rooted resistance to fundamental changes (e.g., global financial crisis, climate change negotiations). In an effort to repair the system as fast as possible, those paradigms, mechanisms, and structures that led into the crisis are perpetuated. Instead of preserving conventional patterns and focusing on continuity, crises could be used as an opportunity for learning, adapting, and entering onto more sustainable pathways. However, there are different ways not only of arguing for sustainable pathways of development but also of conceptualizing...
Tipo: Peer-Reviewed Reports Palavras-chave: Adaptation; Institutions; Persistence; Resilience; Transformation; Water governance.
Ano: 2012
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Development of pollen mediated activation tagging system for Phalaenopsis and Doritaenopsis Electron. J. Biotechnol.
Tsay,Hsin-Sheng; Ho,Hui-Min; Gupta,Sushim K; Wang,Chang-Sheng; Chen,Pei-Tsu; Chen,Emily Chin-Fun.
In the present study, a novel plant transformation system for Doritaenopsis and Phalaenopsis has been developed. The pollen-mediated activation tagging system was established by artificial pollination. The pollens, co-cultured with Agrobacterium tumefaciens strain EHA105 harbouring an activation tagging vector (pTAG-8), were used for pollination. In order to optimize the transformation efficiency, several factors (concentration of A. tumefaciens, concentration of acetosyringone during co-cultivation and the duration of co-cultivation) known to influence Agrobacterium-mediated DNA transfer were examined. A concentration of 0.5-1 x 10(8) CFU/ml for A. tumefaciens, 0.1 mM acetosyringone, and 6 hrs of co-culture period were found to be the optimal condition...
Tipo: Journal article Palavras-chave: Activation tagging; Agrobacterium tumefaciens; Doritaenopsis cultivars; Phalaenopsis amabilis; Transformation.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400009
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Ecosystem Services Linking Social and Ecological Systems: River Brownification and the Response of Downstream Stakeholders Ecology and Society
Tuvendal, Magnus; Department of Systems Ecology, Stockholm University, Sweden; Stockholm Resilience Centre, Stockholm University, Sweden; magnus.tuvendal@ecology.su.se; Elmqvist, Thomas; Department of Systems Ecology, Stockholm University, Sweden; Stockholm Resilience Centre, Stockholm University, Sweden; thomase@ecology.su.se.
Tipo: Peer-Reviewed Reports Palavras-chave: Adaptation; Brownification; Coping; Ecosystem service; Governance; Resilience; Response strategies; Social-ecological system; Transformation.
Ano: 2011
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Emergent transformation games: exploring social innovation agency and activation through the case of the Belgian electricity blackout threat Ecology and Society
The persistence of current societal problems has given rise to a quest for transformative social innovations. As social innovation actors seek to become change makers, it has been suggested that they need to play into impactful macrodevelopments or “game-changers”. Here, we aim to deepen the understanding of the social innovation agency in these transformation games. We analyze assumptions about the game metaphor, invoking insights from actor-network theory. The very emergence of transformation games is identified as a crucial but easily overlooked issue. As explored through the recent electricity blackout threat in Belgium, some current transformation games are populated with largely passive players. This illustrative case demonstrates...
Tipo: Peer-Reviewed Synthesis Palavras-chave: Actor network theory; Electricity grid; Enrolment; Game-changers; Social innovation; Transformation.
Ano: 2016
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ENTWICKLUNGSTENDENZEN LANDWIRTSCHAFTLICHER FAMILIENBETRIEBE IN RUSSLAND SEIT 1990 AgEcon
Katja, Bruisch.
An die russische Agrarreform der 1990er Jahre knüpfte sich die Erwartung, dass entsprechend der Erfahrung in westlichen Staaten landwirtschaftliche Familienbetriebe (Fermer-Betriebe) an die Stelle der staatlichen Großbetriebe treten und einen Aufschwung in der russischen Landwirtschaft auslösen würden. Entgegen dieser Erwartungen ist der russische Fermer-Sektor bis heute verhältnismäßig schwach entwickelt, während die Landwirtschaft als eines der wichtigsten Problemfelder der russischen Innenpolitik gilt. Im vorliegenden Discussion Paper werden die für die Entstehung des Fermer-Sektors relevanten Aspekte der russischen Agrarreform nachvollzogen sowie auf der Grundlage russischer Statistiken die gegenwärtige Lage der russischen Landwirtschaft analysiert. Im...
Tipo: Working or Discussion Paper Palavras-chave: Russland; Transformation; Agrarreform; Familienbetriebe.; Agribusiness; Community/Rural/Urban Development; Farm Management; Industrial Organization; International Development; Political Economy; P 25; Q 12; Q 15.
Ano: 2007 URL: http://purl.umn.edu/90873
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Ethanol mediated enhancement in bacterial transformation Electron. J. Biotechnol.
Sharma,Arun Dev; Singh,Jaspreet; Gill,Prabhjot Kaur.
In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene libraries. The present study demonstrated a new ethanol-based method for transformation of plasmid DNA to E. coli. Ethanol at 10% concentration (v/v) showed best results. Further, as compared with traditional CaCl2 method, the transformation rate, using protocol outlined in this study, was very high, suggesting amenable for further applications.
Tipo: Journal article Palavras-chave: Bacteria; Ethanol; Transformation.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100016
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Exploring institutional adaptive capacity in practice: examining water governance adaptation in Australia Ecology and Society
Bettini, Yvette; University of Queensland, Institute for Social Science Research; Monash Water for Liveability Centre, Cooperative Research Centre for Water Sensitive Cities, Monash University; y.bettini@uq.edu.au; Brown, Rebekah R; School of Social Sciences, Monash Water for Liveability Centre, Cooperative Research Centre for Water Sensitive Cities, Monash University; Rebekah.Brown@monash.edu; de Haan, Fjalar J; School of Social Sciences, Monash Water for Liveability Centre, Cooperative Research Centre for Water Sensitive Cities, Monash University; Fjalar.dehaan@monash.edu.
Adaptive capacity is widely held as a key property of resilient and transformative social-ecological systems. However, current knowledge of the term does not yet address key questions of how to operationalize this system condition to address sustainability challenges through research and policy. This paper explores temporal and agency dimensions of adaptive capacity in practice to better understand how system conditions and attributes enable adaptation. An institutional dynamics lens is employed to systemically examine empirical cases of change in urban water management. Comparative analysis of two Australian cities' drought response is conducted using institutional analysis and qualitative system dynamics mapping techniques. The study finds that three...
Tipo: Peer-Reviewed Reports Palavras-chave: Adaptive capacity; Institutions; Resilience; Transformation; Water governance.
Ano: 2015
Registros recuperados: 65
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