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| Registros recuperados: 62 | |
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| Pollard, Sharon; The Association for Water and Rural Development; sharon@award.org.za; Biggs, Harry; SANParks; Harry.Biggs@sanparks.org; Du Toit, Derick R; The Association for Water and Rural Development; derick@award.org.za. |
| We aimed to contribute to the field of natural resource management (NRM) by introducing an alternative systemic context-based framework for planning, research, and decision making, which we expressed practically in the development of a decision-making “tool” or method. This holistic framework was developed in the process of studying a specific catchment area, i.e., the Sand River Catchment, but we have proposed that it can be generalized to studying the complexities of other catchment areas. Using the lens of systemic resilience to think about dynamic and complex environments differently, we have reflected on the development of a systemic framework for understanding water and livelihood security under transformation in postapartheid... |
| Tipo: Peer-Reviewed Reports |
Palavras-chave: Complexity; Decision making; Dynamic; Governance; IWRM; Livelihood security; Resilience; SES; Social-ecological systems; Transdisciplinarity; Transformation. |
| Ano: 2014 |
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| Carvalho,Carlos Henrique S.; Zehr,Usha B.; Gunaratna,Nilupa; Anderson,Joseph; Kononowicz,Halina H.; Hodges,Thomas K.; Axtell,John D.. |
| The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agrobacterium tumefaciens; GUS; Transformation; Sorghum. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200022 |
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| Tu,Zhiming; He,Guangyuan; Li,Kexiu X.; Chen,Mingjie J.; Chang,Junli; Chen,Ling; Yao,Qing; Liu,Dongping P.; Ye,Huan; Shi,Jiantao; Wu,Xuqian . |
| This paper describes an efficient bacterial transformation system that was established for the preparation of competent cells, plasmid preparation, and for the storage in bacterial stocks in our laboratory. Using this method, a number of different plasmids have been amplified for further experiments. Competent cells for bacterial transformation were prepared by the calcium chloride method with an optimum concentration of 75 mM. Three different strains of Escherichia coli that were tested are DH5α, TG1 and XL1 blue, and the most efficient strain being XL1 blue. The optimal optical density (OD600) range for competent cell preparation varied for each of the strains investigated, and for XL1 blue it was 0.15-0.45; for TG1 it was 0.2-0.5; and for... |
| Tipo: Journal article |
Palavras-chave: Competent cells; E. coli; Plasmid; Storage; Transformation. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100014 |
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| ANDRADE, S. R. M.; GLORIA, B. A.; LABATE, M. T. V.; GOMES, D. G.; LABATE, C. A.. |
| Phaseolus vulgaris L. e considerada recalcitrante a transformacao por Agrobacterium tumefaciens. Contudo, alteracoes no meio de co-cultivo, utilizacao de linhagens hipervirulentas de Agrobacterium e de vetores binarios contendo genes vir demostraram que o feijoeiro e susceptivel a essa bacteria. O objetivo do presente trabalho foi estudar o efeito da sonificacao nos tecidos vegetais de feijoeiro, bem como a penetracao da Agrobacterium nas camadas subepidermicas do tecido vegetal, usando a metodologia SAAT ("Sonification-Assisted Agrobacterium-mediated Transformation"). A variedade de feijoeiro utilizada foi a Olathe Pinto, a linhagem de A. tumefaciens foi LBA4404:pTOK.Os embrioes de feijao foram pre-tratados po 14 dias em meio de multibrotacao e, entao... |
| Tipo: Folhetos |
Palavras-chave: Transformation; SAAT; Common bean; Biotecnologia: transgenico; Bactéria; Cerrado; Feijão; Genética Vegetal; Infecção; Phaseolus Vulgaris; Plant genetics. |
| Ano: 2001 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567067 |
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| Lockwood, Michael; Geography and Environmental Studies, School of Land & Food, University of Tasmania; Michael.Lockwood@utas.edu.au; Mitchell, Michael; Geography and Environmental Studies, School of Land & Food, University of Tasmania; Michael.Mitchell@utas.edu.au; Moore, Susan A.; School of Veterinary and Life Sciences, Murdoch University ; S.Moore@murdoch.edu.au; Clement, Sarah; School of Veterinary and Life Sciences, Murdoch University ; S.Clement@murdoch.edu.au. |
| Biodiversity conservation continues to be a challenging task for societies worldwide. We undertook a resilience assessment to address the following question: What are the ramifications of social-ecological system dynamics for biodiversity governance of a nationally significant landscape? Resilience assessment offers promise for guiding response strategies, potentially enabling consideration of ecological, social, economic, and governance influences on biodiversity-related activities. Most resilience assessments have, however, struggled to effectively incorporate governance influences. We applied a modified version of the Resilience Alliance workbook approach to explicitly address governance influences at each stage of an assessment of internationally... |
| Tipo: Peer-Reviewed Reports |
Palavras-chave: Adaptive cycle; Biodiversity; Climate change; Governance; Resilience assessment; Transformation. |
| Ano: 2014 |
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| TRONCOZO,MARÍA I.; FIGOLI,CECILIA B.; FRANCO,MARIO E.E.; MIRÍFICO,MARÍA V.; BOSCH,ALEJANDRA; RAJCHENBERG,MARIO; BALATTI,PEDRO A.; SAPARRAT,MARIO C. N.. |
| Abstract Grape pomace from Vitis labrusca is an important sub-product of the “American table wine” industry. It is recalcitrant to degradation, and its accumulation is a serious problem with negative environmental impacts. We analyzed the ability of five white-rot fungi to transform this residue in-vitro. Mass loss and phenol removal in grape pomace treated with each fungus were compared after 30-day solid-state fermentation. Since Peniophora albobadia isolate LPSC 285 was the fungus that showed the highest degradative ability and the lowest free phenol levels in the residue transformed, we selected this fungus to monitor its effect on this residue after 30, 60, and 90 days of incubation. We analyzed mass loss of the residue caused by the fungus activity... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fungi; Grape pomace; Peniophora albobadia; Transformation; Vitis labrusca. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100704 |
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| Abel, Nick; Land and Water, Commonwealth Scientific and Industrial Research Organisation ; nick.abel@csiro.au; Wise, Russell M.; Land and Water, Commonwealth Scientific and Industrial Research Organisation; russell.wise@csiro.au; Colloff, Matthew J.; Land and Water, Commonwealth Scientific and Industrial Research Organisation; Matt.Colloff@csiro.au; Walker, Brian H.; Land and Water, Commonwealth Scientific and Industrial Research Organisation; brian.walker@csiro.au; Butler, James R. A.; Land and Water, Commonwealth Scientific and Industrial Research Organisation; james.butler@csiro.au; Ryan, Paul; Australian Resilience Centre; paulryan@internode.on.net; Norman, Chris; Goulburn Broken Catchment Management Authority; chrisn@gbcma.vic.gov.au; Langston, Art; Land and Water, Commonwealth Scientific and Industrial Research Organisation; art.langston@csiro.au; Anderies, John M.; Arizona State University; m.anderies@asu.edu; Gorddard, Russell; Land and Water, Commonwealth Scientific and Industrial Research Organisation; Russell.Gorddard@csiro.au; Dunlop, Michael; Land and Water, Commonwealth Scientific and Industrial Research Organisation; michael.dunlop@csiro.au; O'Connell, Deborah; Land and Water, Commonwealth Scientific and Industrial Research Organisation; deborah.o'connell@csiro.au. |
| Climate change and its interactions with complex socioeconomic dynamics dictate the need for decision makers to move from incremental adaptation toward transformation as societies try to cope with unprecedented and uncertain change. Developing pathways toward transformation is especially difficult in regions with multiple contested resource uses and rights, with diverse decision makers and rules, and where high uncertainty is generated by differences in stakeholders’ values, understanding of climate change, and ways of adapting. Such a region is the Murray-Darling Basin, Australia, from which we provide insights for developing a process to address these constraints. We present criteria for sequencing actions along adaptation pathways: feasibility... |
| Tipo: Peer-Reviewed Insight |
Palavras-chave: Adaptation pathways; Climate change; Collective action; Domain shift; Equity; Irrigation; Resilience; Social conflict; Transformation; Wetlands. |
| Ano: 2016 |
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| Pahl-Wostl, Claudia; Institute for Environmental Systems Research, University of Osnabrueck; pahl@usf.uni-osnabrueck.de. |
| In recent years recurring political, economic, and environmental crises require questioning and re-evaluating dominant pathways of human development. However, political and economic frameworks seem to encompass deeply rooted resistance to fundamental changes (e.g., global financial crisis, climate change negotiations). In an effort to repair the system as fast as possible, those paradigms, mechanisms, and structures that led into the crisis are perpetuated. Instead of preserving conventional patterns and focusing on continuity, crises could be used as an opportunity for learning, adapting, and entering onto more sustainable pathways. However, there are different ways not only of arguing for sustainable pathways of development but also of conceptualizing... |
| Tipo: Peer-Reviewed Reports |
Palavras-chave: Adaptation; Institutions; Persistence; Resilience; Transformation; Water governance. |
| Ano: 2012 |
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| Tsay,Hsin-Sheng; Ho,Hui-Min; Gupta,Sushim K; Wang,Chang-Sheng; Chen,Pei-Tsu; Chen,Emily Chin-Fun. |
| In the present study, a novel plant transformation system for Doritaenopsis and Phalaenopsis has been developed. The pollen-mediated activation tagging system was established by artificial pollination. The pollens, co-cultured with Agrobacterium tumefaciens strain EHA105 harbouring an activation tagging vector (pTAG-8), were used for pollination. In order to optimize the transformation efficiency, several factors (concentration of A. tumefaciens, concentration of acetosyringone during co-cultivation and the duration of co-cultivation) known to influence Agrobacterium-mediated DNA transfer were examined. A concentration of 0.5-1 x 10(8) CFU/ml for A. tumefaciens, 0.1 mM acetosyringone, and 6 hrs of co-culture period were found to be the optimal condition... |
| Tipo: Journal article |
Palavras-chave: Activation tagging; Agrobacterium tumefaciens; Doritaenopsis cultivars; Phalaenopsis amabilis; Transformation. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400009 |
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| Tuvendal, Magnus; Department of Systems Ecology, Stockholm University, Sweden; Stockholm Resilience Centre, Stockholm University, Sweden; magnus.tuvendal@ecology.su.se; Elmqvist, Thomas; Department of Systems Ecology, Stockholm University, Sweden; Stockholm Resilience Centre, Stockholm University, Sweden; thomase@ecology.su.se. |
| Tipo: Peer-Reviewed Reports |
Palavras-chave: Adaptation; Brownification; Coping; Ecosystem service; Governance; Resilience; Response strategies; Social-ecological system; Transformation. |
| Ano: 2011 |
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| The persistence of current societal problems has given rise to a quest for transformative social innovations. As social innovation actors seek to become change makers, it has been suggested that they need to play into impactful macrodevelopments or “game-changers”. Here, we aim to deepen the understanding of the social innovation agency in these transformation games. We analyze assumptions about the game metaphor, invoking insights from actor-network theory. The very emergence of transformation games is identified as a crucial but easily overlooked issue. As explored through the recent electricity blackout threat in Belgium, some current transformation games are populated with largely passive players. This illustrative case demonstrates... |
| Tipo: Peer-Reviewed Synthesis |
Palavras-chave: Actor network theory; Electricity grid; Enrolment; Game-changers; Social innovation; Transformation. |
| Ano: 2016 |
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| Bettini, Yvette; University of Queensland, Institute for Social Science Research; Monash Water for Liveability Centre, Cooperative Research Centre for Water Sensitive Cities, Monash University; y.bettini@uq.edu.au; Brown, Rebekah R; School of Social Sciences, Monash Water for Liveability Centre, Cooperative Research Centre for Water Sensitive Cities, Monash University; Rebekah.Brown@monash.edu; de Haan, Fjalar J; School of Social Sciences, Monash Water for Liveability Centre, Cooperative Research Centre for Water Sensitive Cities, Monash University; Fjalar.dehaan@monash.edu. |
| Adaptive capacity is widely held as a key property of resilient and transformative social-ecological systems. However, current knowledge of the term does not yet address key questions of how to operationalize this system condition to address sustainability challenges through research and policy. This paper explores temporal and agency dimensions of adaptive capacity in practice to better understand how system conditions and attributes enable adaptation. An institutional dynamics lens is employed to systemically examine empirical cases of change in urban water management. Comparative analysis of two Australian cities' drought response is conducted using institutional analysis and qualitative system dynamics mapping techniques. The study finds that three... |
| Tipo: Peer-Reviewed Reports |
Palavras-chave: Adaptive capacity; Institutions; Resilience; Transformation; Water governance. |
| Ano: 2015 |
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| Registros recuperados: 62 | |
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