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Biolistic-mediated transient gene expression in shoot apical meristems of the prickly-pear (Opuntia ficus-indica) BABT
Llamoca-Zárate,Romulo Marino; Ponte,Luiz Ferreira Aguiar; Landsmann,Joerg; Campos,Francisco de Assis Paiva.
We have demonstrated the transient expression of the GUS gene in cells of the meristematic apical dome of Opuntia ficus-indica. DNA delivery into the cells was achieved using a biolistic PDS-1000He instrument from Bio-Rad Laboratories. The transforming DNA was coated in tungsten particles with diameter of 1.3 m m and the distance between the flying disk and the target tissue was 7.5cm and the shooting pressure was adjusted to 1200 psi. This is the first demonstration that the biolistic transformation system can be used to express a transgene in a member of the Cactaceae.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Opuntia ficus-indica; Prickly-pear; Cactaceae; Transient gene expression; Particle bombardment.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300005
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Expression of GUS-gene in monocot temperate grasses by an improved particle gun OAK
HORIKAWA, Yoh; OHI, Hiroyuki; KAKUTA, Hideo; 堀川, 洋; 大井, 弘幸; 角田, 英男.
http://www.obihiro.ac.jp/~library/kenkyu.html
Palavras-chave: Particle gun; Transient gene expression; GUS (β-Gulcuronidase); Dactylisglomerata L.; Phleum pratense L..
Ano: 1993 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3764
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Gene delivery into plant cells and magnetic separation using magnetite particles OAK
Yoshizumi, Takeshi; Horikawa, Yoh; Kakuta, Hideo; 吉積, 毅; 堀川, 洋; 角田, 英男.
Palavras-chave: Β-glucruronidase (GUS) gene; Magnetite; Magnetic separation; Particle bombardment; Transient gene expression; 一過的遺伝子発現; 磁気分離; 磁性体; 微粒子撃ち込み; Β-グルクロニダーゼ(GUS)遺伝子.
Ano: 1996 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1343
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Improving and assessing viral vectors for recombinant protein production in plants. Repositório Alice
LACORTE, C..
Chapter 1 - Introduction; Chapter 2 - PVX and TMV -based viral vectors compatible with the GatewayTM cloning technology; Chapter 3 - Expression and localization of Chicken anemia virus (CA V) proteins in plant cells; Chapter 4 - High expression of recombinant human erythropoietin in plants using TMV and PVX-based viral vectors; Chapter 5 - Genetic stability and spatial distribution ofTobacco mosaic virus-based vectors in Nicotiana benthamiana plants; Chapter 6 - The nucleoprotein of Tomato spotted wilt virus as protein tag for easy purification and enhanced production of recombinant proteins in plants; Chapter 7 - General discussion; References. Summary. Samenvatting. About the author. Acknowledgements. Appendix (colou r figures).
Tipo: Tese/dissertação (ALICE) Palavras-chave: Viral vectors; Transient gene expression; Plant-made proteins; Erythropoietin; Gene fusion; Chicken anemia virus.
Ano: 2006 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/188426
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Isolation and regeneration of transiently transformed protoplasts from gametophytic blades of the marine red alga Porphyra yezoensis Electron. J. Biotechnol.
Takahashi,Megumu; Uji,Toshiki; Saga,Naotsune; Mikami,Koji.
Despite the recent progress of transient gene expression systems in a red alga Porphyra yezoensis by particle bombardment, a stable transformation system has yet to establish in any marine red macrophytes. One of the reasons of the difficulty in genetic transformation in red algae is the lack of systems to select and isolate transformed cells from gametophytic blades. Thus, toward the establishment of the stable transformation system in P. yezoensis, we have developed a procedure by which transiently transformed gametophytic cells were prepared from particle bombarded-gametophytic blade as regeneratable protoplasts. Using mixture of marine bacterial enzymes, yield of protoplasts was high as reported elsewhere; however, these protoplasts did not develop. In...
Tipo: Journal article Palavras-chave: Allantoin; Protoplast; P. yezoensis; Regeneration; Transient gene expression.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200008
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Optimization of polyethylenimine-mediated transient transfection using response surface methodology design Electron. J. Biotechnol.
Fang,Qiangyi; Shen,Bingqian.
Response surface methodology was undertaken to optimize the polyethylenimine-mediated transient transfection of suspension cultured HEK 293-F cells. A total of 15 combinations were designed according to Box-Behnken design to identify the effects of DNA concentration, polyethylenimine concentration and incubation time on transient transfection efficiency. The highest integral optic density of green fluorescent protein presenting r-protein yield was accessed using a DNA concentration of 1.75 µg/mL, a polyethylenimine concentration of 10.5 µg/mL, and an incubation time of 11.8 min. Analysis of variance demonstrated that the experimental values fit well with a quadratic model. The RSM-optimized transient transfection resulted in greater production of human...
Tipo: Journal article Palavras-chave: Bioreactor; Human tissue kallikrein; Transient gene expression.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500010
Registros recuperados: 6
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