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Avian Paramyxoviruses: Detection by Transmission Electron Microscopy Techniques 77
Catroxo,M. H. B; Martins,A. M. C. P. F; Petrella,S; Milanelo,L; Aschar,M; Souza,F; Nastari,B.D.B; Souza,R.B.
Diseases caused by avian paramyxovirus (APMV) occur in commercial, captive and wild birds worldwide, demonstrating the significant economic and ecological importance of these agents. Paramyxoviruses belong to the Paramyxoviridae family, Paramyxovirinae subfamily and Avulavirus genus. During the period 2000 to 2011, stool and small intestine samples of 1647 birds species were sent to the Laboratory of Electron Microscopy, Biological Institute of São Paulo, Brazil, for diagnosis of viral agents. The samples were processed by negative staining (rapid preparation) and resin embedding techniques. Under the transmission electron microscope by negative staining technique, in 294 (17.8%) samples of 1647 were visualized paramyxovirus particles pleomorphic, roughly...
Tipo: Journal article Palavras-chave: Paramyxoviruses; Avian; Transmission Electron Microscopy.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200062
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Diagnosis to Detect Porcine Transmissible Gastroenteritis Virus (TGEV) by Optical and Transmission Electron Microscopy Techniques 77
Martins,A. M. C. R. P. F; Bersano,J. G; Ogata,R; Amante,G; Nastari,B. D. B; Catroxo,M. H. B.
The porcine transmissible gastroenteritis is a highly severe contagious disease, caused by virus of the Coronaviridae family, genus Coronavirus. Its epizootic shape can reach a rate of up to 100% mortality in piglets under two weeks of age as a result of severe dehydration. In this study fragments of small intestine and stool samples were collected from 75 autopsied pigs from properties. The samples of the fragments were frozen and sent to the Laboratory of Electron Microscopy, Instituto Biológico, SP, Brazil, for histological and transmission electron microscopic analyses. According to histological H&E technique, atrophy, villous necrosis and destruction of the enterocytes were observed in 35 (46.6%) out of the 75 fragments of the small intestine...
Tipo: Journal article Palavras-chave: Porcine Transmissible Gastroenteritis; Coronavirus; Histopathology; Transmission Electron Microscopy.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200059
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Ultrastructural Study of Bovine Papillomavirus During Outbreaks in Brazil 77
Catroxo,M. H. B; Martins,A. M. C. R. P. F; Petrella,S; Souza,F; Nastari,B.D.B.
Papillomaviruses, belonging to the Papillomaviridae family, are small oncogenic viruses, causing papillomas and fibropapillomas in the mucosal and cutaneous epithelia of several animals. In bovine species, thirteen types (BPV 1-13) were characterized to date. In this study, the occurrence of papillomatosis in four outbreaks in cattle herds, coming from Brazilian states were registered. The papillomatous lesions were found located in the teats, udders, head and neck. Under the transmission electron microscope, by the negative staining technique, it was possible to visualized rounded-format papillomavirus, with icosahedral symmetry, characterized as "full" and "empty" particles, measuring on average 60 nm in diameter, in all the 40 samples observed of skin...
Tipo: Journal article Palavras-chave: Papillomavirus; Cattle; Transmission Electron Microscopy.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200068
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Ultrastructural study of the mycelial phase of clinical isolates of Sporothrix schenckii obtained from feline, canine and human cases of sporotrichosis 58
Madrid,Isabel Martins; Mattei,Antonella Souza; Soares,Mauro Pereira; Nobre,Márcia de Oliveira; Meireles,Mário Carlos Araújo.
Using transmission electron microscopy, we studied the presence of melanin and cell wall thickness of clinical isolates of Sporothrix schenckii obtained from cats, dogs and humans as compared to reference strains. We detected differences regarding presence of the melanin among the clinical isolates of S. schenckii and a correlation between presence of melanin and cell wall thickness.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conidial; Melanin; Sporothrix schenckii; Transmission Electron Microscopy.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300037
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