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A Blower-like approach to predict the effectiveness of vaccines in a TB dynamic. Repositório Alice
FRONZA, C. F.; ANSELMO, J.; MARQUES, J. L. B.; PINTARELLI, G. B.; CASTRO, A. de..
In this paper we present an extension of an automata approach proposed by S. Blower (1998) to describe the tuberculosis progression in a bi-dimensional space. In our extended model, the vaccination was included as an inhibitory variable in order to study its influence on the behavior of the tuberculosis spread. Our simulations showed that the earlier the vaccine is administered in the population, the lower the number of infected individuals, as expected for an in vivo system. However, our results also indicated that although the usual vaccination processes help reducing the strength of infection, the disease is not extinct, remaining the endemic state at low levels. These results strongly suggest that further actions are needed to increase the...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Simulação; Cellular automata; Tuberculose; Tuberculosis; Computer simulation.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/990390
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A lung image reconstruction from computed radiography images as a tool to tuberculosis treatment control J. Venom. Anim. Toxins incl. Trop. Dis.
Oliveira,Marcela de; Duarte,Sergio Barbosa; Giacomini,Guilherme; Pereira,Paulo Câmara Marques; Souza,Lenice do Rosário de; Miranda,José Ricardo de Arruda; Pina,Diana Rodrigues de.
Abstract Background: Background: Tuberculosis (TB) is an infectious lung disease with high worldwide incidence that severely compromises the quality of life in affected individuals. Clinical tests are currently employed to monitor pulmonary status and treatment progression. The present study aimed to apply a three-dimensional (3D) reconstruction method based on chest radiography to quantify lung-involvement volume of TB acute-phase patients before and after treatment. In addition, these results were compared with indices from conventional clinical exams to show the coincidence level. Methods: A 3D lung reconstruction method using patient chest radiography was applied to quantify lung-involvement volume using retrospective examinations of 50 patients who...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; Quantification; Lung involvement; Serum tests; Pulmonary function tests.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100302
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A single strain of Mycobacterium bovis bacillus Calmette-Guérin (BCG) grown in two different media evokes distinct humoral immune responses in mice BJMBR
Petricevich,V.L.; Ueda,C.; Alves,R.C.B.; da Silva,M.A.; Moreno,C.; Melo,A.R.; Dias da Silva,W..
Two attenuated bacillus Calmette-Guérin (BCG) preparations derived from the same Moreau strain, Copenhagen but grown in Sauton medium containing starch and bacto-peptone (onco BCG, O-BCG), or asparagine (intradermal BCG, ID-BCG), exhibited indistinguishable DNA sequences and bacterial morphology. The number of viable bacilli recovered from spleen, liver and lungs was approximately the same in mice inoculated with the vaccines and was similarly reduced (over 90%) in mice previously immunized with either BCG vaccine. The humoral immune response evoked by the vaccines was, however, distinct. Spleen cell proliferation accompanying the growth of bacilli in tissue was significantly higher in mice inoculated with O-BCG. These cells proliferated in vitro upon...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus Calmette-Guérin; BCG; Tuberculosis; Mycobacterium bovis; Vaccine.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100010
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A study of iga antibody response to different mycobacterium tuberculosis antigens in the diagnosis and monitoring of pulmonary tuberculosis BJID
Bezerra,Janaina Miranda; Beck,Sandra Trevisan; Kanunfre,Kelly Aparecida; Leite,Olavo Munhoz; Ferreira,Antônio Walter.
We evaluated the performance of the ELISA technique in the detection of IgA antibodies against different Mycobacterium tuberculosis antigenic preparations in serum samples from 49 patients with pulmonary tuberculosis collected before and after the start of specific treatment. The controls consisted of serum samples from healthy patients without any prior contact with the bacteria and serum samples from patients with other pneumopathies. Glycolipid antigen gave the best diagnostic performance, with a sensitivity of 88% and specificities varying from 88 to 100% in the control groups. These antigens constitute a powerful tool for the diagnosis and monitoring of patients with pulmonary tuberculosis.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; IgA; Serological diagnosis; ELISA; Glycolipid antigen.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000100012
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A two-step strategy for the complementation of M: tuberculosis mutants Genet. Mol. Biol.
Movahedzadeh,Farahnaz; Frita,Rosangela; Gutka,Hiten J..
The sequence of Mycobacterium tuberculosis, completed in 1998, facilitated both the development of genomic tools, and the creation of a number of mycobacterial mutants. These mutants have a wide range of phenotypes, from attenuated to hypervirulent strains. These phenotypes must be confirmed, to rule out possible secondary mutations that may arise during the generation of mutant strains. This may occur during the amplification of target genes or during the generation of the mutation, thus constructing a complementation strain, which expresses the wild-type copy of the gene in the mutant strain, becomes necessary. In this study we have introduced a two-step strategy to construct complementation strains using the Ag85 promoter. We have constitutively...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; Mutagenesis; Constitutive expression; Complementation.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200020
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Antigen-presenting cells transfected with Hsp65 messenger RNA fail to treat experimental tuberculosis BJMBR
Rocha,C.D.; Trombone,A.P.F.; Lorenzi,J.C.C.; Almeida,L.P.; Gembre,A.F.; Padilha,E.; Ramos,S.G.; Silva,C.L.; Coelho-Castelo,A.A.M..
In the last several years, the use of dendritic cells has been studied as a therapeutic strategy against tumors. Dendritic cells can be pulsed with peptides or full-length protein, or they can be transfected with DNA or RNA. However, comparative studies suggest that transfecting dendritic cells with messenger RNA (mRNA) is superior to other antigen-loading techniques in generating immunocompetent dendritic cells. In the present study, we evaluated a new therapeutic strategy to fight tuberculosis using dendritic cells and macrophages transfected with Hsp65 mRNA. First, we demonstrated that antigen-presenting cells transfected with Hsp65 mRNA exhibit a higher level of expression of co-stimulatory molecules, suggesting that Hsp65 mRNA has immunostimulatory...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; Therapeutic immunization; CD; MHSP65; DNA vaccine; APCs.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200011
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Anti-tuberculosis drug resistance in strains of Mycobacterium tuberculosis isolated from patients in a tertiary hospital in Bahia BJID
Matos,Eliana Dias; Lemos,Antônio Carlos Moreira; Bittencourt,Carolina; Mesquita,Cristiane Leite.
Anti-tuberculosis drug resistance, particularly multiresistance, is a crucial issue in the control of tuberculosis (TB). This study estimated the prevalence of primary and acquired anti-tuberculosis drug resistance in strains of Mycobacterium tuberculosis isolated from hospitalized patients, to identify the risk factors for resistance, and to evaluate the its impact on hospital mortality for tuberculosis. Strains of Mycobacterium tuberculosis from 217 patients hospitalized for TB were analyzed. Subjects were recruited sequentially at a TB reference hospital in Salvador, Bahia, Brazil from July 2001 to July 2003. Multiresistant (MR) strains were defined as strains resistant to rifampicin and isoniazid. Of a total of 217 strains isolated, 41 (19.0%, 95%CI:...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium tuberculosis; Tuberculosis; Resistance; Multiresistance; Hospitals; Bahia-Brazil.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300007
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Assessing new strategies for TB diagnosis in low- and middle-income countries BJID
Kritski,Afrânio; Fujiwara,Paula I.; Vieira,Maria Armanda; Netto,Antonio Ruffino; Oliveira,Martha Maria; Huf,Gisele; Squire,S. Bertel.
In this manuscript, we report the current situation of tuberculosis globally and in Brazil, the need for new strategies toward tuberculosis control, focusing on new diagnostic technologies. Critical comments are given on the state of the art regarding the evaluation of new health technologies, degree of scientific evidence needed, evaluation of clinical impact, cost-effectiveness of incorporation into the health system and the social impact.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; Diagnostics; Impact; Evidence; Policy; Innovation; Regulatory agency.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200015
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Assessment of the effectiveness of a home-based care program for patients coinfected with tuberculosis and human immunodeficiency virus after discharge from a reference hospital in South-Eastern Brazil BJID
Bento,Carlos Alessandro Plá; Pedroso,Enio Roberto Pietra.
The effectiveness of tuberculosis treatment delivered by a home-based care program to patients coinfected with HIV was compared with that of a service provided by outpatient departments. A retrospective study was made of a cohort of coinfected patients discharged from hospital between January 1998 and December 2002 who had been followed-up for one year within one of these programs. Two-hundred-and-forty-three patients who met the inclusion criteria were grouped according to their treatment program (group 1 received home-based care; group 2 attended outpatient departments) and then paired one-to-one across the groups by gender, age and level of education. Only 48 patients from each group could be paired. Apart from the duration of HIV infection, there were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AIDS; Tuberculosis; Treatment outcome; Adherence to treatment; Home-based care.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600008
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Association between toll-like receptor2 Arg677Trp and 597T/C gene polymorphisms and pulmonary tuberculosis in Zahedan, Southeast Iran BJID
Naderi,Mohammad; Hashemi,Mohammad; Hazire-Yazdi,Leylisadat; Taheri,Mohsen; Moazeni-Roodi,Abdolkarim; Eskandari-Nasab,Ebrahim; Bahari,Gholamreza.
BACKGROUND: It is well known that toll-like receptor 2 (TLR2) mediates responses of both innate and adaptive immunity to microbial pathogen, including mycobacteria. Single-nucleotide polymorphisms (SNPs) in the TLR2 gene that impair its function may be associated with the development of pulmonary tuberculosis (PTB). The aim of this study was to evaluate the possible association between TLR2 Arg677Trp and 597T/C polymorphisms and PTB in a sample of Iranian population. MATERIALS AND METHODS: This case-;control study was performed on 174 PTB and 177 healthy subjects. Tetra amplification refractory mutation system-polymerase chain reaction (TARMS-PCR) was used to detect the SNPs. RESULTS: There was no significant difference in the polymorphism of Arg677Trp of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; TLR2; Single nucleotide polymorphism.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000500002
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Association of genetic polymorphisms of CISH with the risk of pulmonary tuberculosis in Zahedan, Southeast Iran BJID
Naderi,Mohammad; Hashemi,Mohammad; Safdari,Abolhassan; Bahari,Gholamreza; Taheri,Mohsen.
Abstract Background In the current study we aimed to find out the impact of cytokine-inducible Src homology 2 domain protein (CISH) gene polymorphisms on the risk of pulmonary tuberculosis (PTB) in a sample of Iranian population. Materials and methods Polymorphisms of CISH rs2239751, rs414171, and rs6768300 were determined in 200 PTB patients and 200 healthy subjects using T-ARMS-PCR or PCR-RFLP method. Results The results showed that rs414171 A>T genotypes significantly decreased the risk of PTB (OR = 0.16, 95% CI = 0.10–0.27, p < 0.0001, AT vs AA; OR = 0.31, 95% CI = 0.14–0.68, p < 0.0001, TT vs AA; OR = 0.19, 95% CI = 0.12–0.29, p < 0.0001, AT+TT vs AA; OR = 0.29, 95%CI = 0.20–0.42, p < 0.0001, T vs A). For rs6768300, the findings...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; CISH; Polymorphism.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000400379
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Bronchoalveolar lavage (BAL) in tapirs (Tapirus terrestris) with Mycobacterium tuberculosis Arq. Bras. Med. Vet. Zootec.
Biava,J.S.; Gonçalves,R.C.; Javorouski,M.L.; Bonat,M.; Lacerda,O.; Murakami,P.S.; Vilani,R.G.D.C.; Biondo,A.W..
This paper describes the in vivo Bronchoalveolar lavage (BAL) technique by endoscopy in tapirs (Tapirus terrestris) with clinical signs of tuberculosis. The technique was performed in two tapirs, male and female, from Curitiba Zoo, Paraná, Brazil. A flexible endoscope and a polyethylene catheter were used after the chemical restraint of the animals. For BAL technique, 60mL of saline 0.9% were infused with a polyethylene catheter, introduced by the endoscope's working channel, and 15mL of BAL were recovered, analyzed and submitted to cytocentrifugation. Slides were stained by Papanicolaou, periodic acid-Schiff (PAS) and Ziehl-Neelsen methods contained high quantity of inflammatory cells on light microscopy (macrophages 27.5%, lymphocytes 0.5%, neutrophis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tapirs; Diagnosis; Endoscopy; Tuberculosis.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601643
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Brucelose e tuberculose bovina: epidemiologia, controle e diagnóstico. Infoteca-e
ALMEIDA, R. F. C. de; SOARES, C. O.; ARAÚJO, F. R. de.
Cap. 1: Brucelose bovina: epidemiologia e controle. Cap. 2: Brucelose bovina: diagnóstico. Cap. 3: Tuberculose bovina: epidemiologia e controle. Cap. 4: Tiberculose bovina: diagnóstico.
Tipo: Livro técnico (INFOTECA-E) Palavras-chave: Sanidade animal; Bovino; Doença animal; Brucelose; Tuberculose; Epidemiologia; Diagnóstico; Controle; Animal health; Cattle; Animal diseases; Brucellosis; Tuberculosis; Diagnosis; Control; Epidemiology.
Ano: 2004 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/325789
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Case report of lymph nodal, hepatic and splenic tuberculosis in an HIV-positive patient BJID
Barone,Bianca; Kreuzig,Patrícia Lago; Gusmão,Patricia Medeiros; Chamié,Daniel; Bezerra,Sabrina; Pinheiro,Pedro; Coscarelli,Pedro; Paiva,Daurita; Fonseca,Leila; Marsico,Anna; Cirigliano,André; Perez,Mário.
We describe a case of a male patient, 38 years old, HIV-positive (most recent CD4 count about 259/mm³), with abdominal pain, nausea, vomiting, anorexia, weight loss, and vespertine high fever with chills. His hemogram showed normocytic and normochromic anemia, with a high erythrocyte sedimentation rate (ESR) and gross granulations in the neutrophils. Transaminases were normal. Bone marrow biopsy evidenced a chronic disease anemia pattern and a lack of infectious agents. Abdominal ultrasound examination showed a normal-size spleen, which exhibited heterogeneous parenchyma and multiple small hypoechoic images, together with small ascites, peripancreatic and para-aortic lymphadenopathy. These findings were confirmed by abdominal CT. The liver was normal in...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Tuberculosis; HIV; Treatment.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000200014
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CD4+ cell counts in patients with different clinical manifestations of tuberculosis BJID
Davoudi,Setareh; Rasoolinegad,Mehrnaz; Younesian,Masoud; Hajiabdolbaghi,Mahboubeh; Soudbakhsh,Abdolreza; Jafari,Sirous; EmadiKouchak,Hamid; Mehrpouya,Morteza; Lotfi,Hossein.
Tuberculosis is the prototype of infections that require a cellular immune response for their control. It has been shown that CD4+ T-lymphocytes are most important in the protective response against Mycobacterium tuberculosis. CD8+ T-lymphocytes are also important for effective T-cell immune response. This study compares CD4+ and CD8+ baseline values in patients with different manifestations of tuberculosis. CD4+ and CD8+ in three groups of patients with tuberculosis (pulmonary, lymphadenitis, meningitis/milliary involvement) and a group of healthy volunteers were enumerated using flowcytometry. Twenty-six patients with pulmonary tuberculosis, 10 with adenitis, 16 with meningitis or milliary tuberculosis and 16 healthy volunteers entered the study. Mean...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; Mycobacterium tuberculosis; CD4.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600008
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Cellular profile of mares colostrum: a preliminary study MV&Z
Simon, Bruna Busianov Zaharov; Roncati, Neimar Vanderlei; Hoge, Alma Yasodhara Alexandra; Porto, Ana Carolina Rusca Correa.
There are few reports on which leukocytes are present in the colostrum and milk of mares. Recent studies in farm animals suggest a role for colostral leukocytes in the transfer of passive immunity from dam to neonate. The aim of this study was to characterize the population of leukocytes presentin colostrum from clinically healthy mares, using the light microscopy. Colostrum samples were collected from 20 mares,12 Mangalarga Marchador and 8 Quarter Horse. Macrophages were the predominant cell type in most samples studied followed by neutrophils and lymphocytes. These results may be a starting point for future studies investigating the role of colostral leukocytes in the transfer of immunity from mare to foal.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; Zoonoses; Human health; Mycobacterium bovis Colostro; Potros; Transferência de imunidade passiva; Leucócitos.
Ano: 2013 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/253
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Challenges in the development of drugs for the treatment of tuberculosis BJID
Shehzad,Adeeb; Rehman,Gauhar; Ul-Islam,Mazhar; Khattak,Waleed Ahmad; Lee,Young Sup.
Tuberculosis infection is a serious human health threat and the early 21st century has seen a remarkable increase in global tuberculosis activity. The pathogen responsible for tuberculosis is Mycobacterium tuberculosis, which adopts diverse strategies in order to survive in a variety of host lesions. These survival mechanisms make the pathogen resistant to currently available drugs, a major contributing factor in the failure to control the spread of tuberculosis. Multiple drugs are available for clinical use and several potential compounds are being screened, synthesized, or evaluated in preclinical or clinical studies. Lasting and effective achievements in the development of anti-tuberculosis drugs will depend largely on the proper understanding of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibiotics; Drug resistance; Tuberculosis; Pathogen.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000100012
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Challenging Mycobacterium tuberculosi s dormancy mechanisms and their immunodiagnostic potential BJID
Chaves,Alexandre Silva; Rodrigues,Michele Fernandes; Mattos,Ana Márcia Menezes; Teixeira,Henrique Couto.
ABSTRACT Mycobacterium tuberculosis is the etiologic agent of tuberculosis, one of the world's greatest cause of morbidity and mortality due to infectious disease. Many evolutionary mechanisms have contributed to its high level of adaptation as a host pathogen. Prior to become dormant, a group of about 50 genes related to metabolic changes are transcribed by the DosR regulon, one of the most complex and important systems of host-pathogen interaction. This genetic mechanism allows the mycobacteria to persist during long time periods, establishing the so-called latent infection. Even in the presence of a competent immune response, the host cannot eliminate the pathogen, only managing to keep it surrounded by an unfavorable microenvironment for its growth....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; Latency; DosR antigens; Diagnosis.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600636
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Characterization of Mycobacterium tuberculosis complex isolated from iranian and afghani patients by spoligotyping method BJM
Ramazanzadeh,Rashid; Farnia,Parisa; Amirmozafari,Nour.
Designing newer drugs, vaccines, and diagnostic techniques is dependent on better understanding of M. tuberculosis virulence mechanism. In this study the prevalence of pcaA gene was determined in M. tuberculosis strains typed by spoligotyping. The associated risk factors among patients with different nationalities residing in Iran were also determined. The isolated M. tuberculosis strains have been characterized by performing susceptibility tests against four first-line antituberculosis drugs and were then subjected to spoligotyping characterization. PCR was used for detection of pcaA gene and its nucleotide sequence was also determined. Spoligotyping of M. tuberculosis strains resulted in 140 different patterns. One hundred twenty two (87.1%) of these...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; Resistance; Drugs; Spoligotyping; PcaA.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200019
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Chronic symptoms and pulmonary dysfunction in post-tuberculosis Brazilian patients BJID
Nihues,Simone de Sousa Elias; Mancuzo,Eliane Viana; Sulmonetti,Nara; Sacchi,Flávia Patussi Correia; Viana,Vanessa de Souza; Martins Netto,Eduardo; Miranda,Silvana Spindola; Croda,Julio.
ABSTRACTBACKGROUND: Questionnaire and spirometry were applied to post-tuberculosis indigenous and non-indigenous individuals from Dourados, Brazil, to investigate the prevalence of chronic respiratory symptoms and pulmonary dysfunction.METHODS:This was a cross-sectional study in cured tuberculosis individuals as reported in the National System on Reportable Diseases (SINAN) from 2002 to 2012.RESULTS:One hundred and twenty individuals were included in the study and the prevalence of chronic respiratory symptoms was 45% (95% CI, 34-59%). Respiratory symptoms included cough (28%), sputum (23%), wheezing (22%) and dyspnea (8%). These symptoms were associated with alcoholism, AOR: 3.1 (1.2-8.4); less than 4 years of schooling, AOR: 5.0 (1.4-17.7); and previous...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazil; Dysfunction; Spirometry; Tuberculosis.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000500492
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