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Registros recuperados: 7
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Comparative analysis of deformed wing virus (DWV) RNA in Apis mellifera and Varroa destructor Inra
Tentcheva, D.; Gauthier, L.; Bagny, L.; Fievet, J.; Dainat, B.; Cousserans, F.; Colin, M. E.; Bergoin, M..
A two step quantitative RT-PCR assay was validated to monitor the deformed wing virus (DWV) RNA loads in Apis mellifera L. and Varroa destructor. A pair of primers hybridising in a conserved domain of the putative DWV RNA polymerase gene region was designed. These primers amplified a 69-nucleotide fragment which was quantified using the SYBR-green chemistry. The experimental validation of the method showed that the RNA extraction and cDNA synthesis steps were responsible for the greatest variability in the results while assays repeated on different PCR plates were reproducible. Quantitative RTPCR analysis on drone bee prepupae showed that DWV RNA loads were higher in cells parasitized by several mother mites. In workers, DWV prevalence was directly...
Tipo: Journal Article Palavras-chave: DEFORMED WING VIRUS DWV; BEE VIRUS; VARROA DESTRUCTOR; QUANTITATIVE PCR.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200885085151&uri=/notices/prodinra1/2009/12/
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Differential gene expression of the honey bee Apis mellifera associated with Varroa destructor infection Inra
Navajas, M.; Migeon, A.; Alaux, C.; Martin-Magniette, M.L.; Robinson, G.E.; Evans, J.D.; Cros-Arteil, S.; Crauser, D.; Le Conte, Y..
Background: The parasitic mite, Varroa destructor, is the most serious pest of the western honey bee, Apis mellifera, and has caused the death of millions of colonies worldwide. This mite reproduces in brood cells and parasitizes immature and adult bees. We investigated whether Varroa infestation induces changes in Apis mellifera gene expression, and whether there are genotypic differences that affect gene expression relevant to the bee's tolerance, as first steps toward unravelling mechanisms of host response and differences in susceptibility to Varroa parasitism. Results: We explored the transcriptional response to mite parasitism in two genetic stocks of A. mellifera which differ in susceptibility to Varroa, comparing parasitized and non-parasitized...
Tipo: Journal Article Palavras-chave: ABEILLE DOMESTIQUE; APIS MELLIFERA; ACARIEN PARAISITE; VARROA DESTRUCTOR.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20095dd5dfa6&uri=/notices/prodinra1/2010/12/
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Mites and ticks (Acari) Inra
Navajas Navarro, M.; Migeon, A.; Estrada-Peña, A.; Mailleux, A.C.; Servigne, P.; Petanović, R..
The inventory of the alien Acari of Europe includes 96 species alien to Europe and 5 cryptogenic species. Among the alien species, 87 are mites and 9 tick species. Besides ticks which are obligate ectoparasites, 14 mite species belong to the parasitic/predator regime. Among these species, some invaded Europe with rodents (8 spp.) and others are parasitic to birds (2 spp). Th e remaining 77 mite species are all phytophagous and among these 40% belong to the Eriophyidae (37 spp.) and 29% to the Tetranychidae (27 spp.) families. Th ese two families include the most signifi cant agricultural pest. Th e rate of introductions has exponentially increased within the 20th century, the amplifi cation of plant trade and agricultural commodities movements being the...
Tipo: Book Chapter Palavras-chave: ALIEN; MITE; TICK; ACARI; ERIOPHYIDAE; ; TETRANYCHIDAE; ; BIOLOGICAL CONTROL; TETRANYCHUS EVANSI; OLIGONYCHUS PERSEAE; POLYPHAGOTARSONEMUS LATUS; BREVIPALPUS CALIFORNICUS; ACERIA SHELDONI; ACULOPS PELEKASSI; DERMATOPHAGOIDES; EVANSI; VARROA DESTRUCTOR.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20102ae85b9&uri=/notices/prodinra1/2011/01/
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Parasitism in the social bee Apis mellifera: quantifying costs and benefits of behavioral resistance to Varroa destructor mites Inra
Vandame, R.; Morand, S.; Colin, M.E.; Belzunces, L.P..
Tipo: Journal Article Palavras-chave: INSECTE UTILE; INSECTE SOCIAL; APIS MELLIFERA; APIDAE; HYMENOPTERA; PARASITISME; ECTOPARASITE; ACARI; MESOSTIGMATA; VARROA DESTRUCTOR; RESISTANCE COMPORTEMENTALE; COUT DE LA RESISTANCE; BENEFICE DE LA RESISTANCE.
Ano: 2002 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0300027650095209&uri=/notices/prodinra1/2009/12/
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Polymerase Chain Reaction detection of deformed wing virus (DWV) in Apis mellifera and Varroa destructor Inra
Tentcheva, D.; Gauthier, L.; Jouve, S.; Canabady-Rochelle, L.; Dainat, B.; Cousserans, F.; Colin, M. E.; Ball, B.V.; Bergoin, M..
We have developed a specific assay for the detection of deformed wing virus (DWV) in Apis mellifera L. and Varroa destructor based on the reverse transcriptase polymerase chain reaction (RT-PCR) technology. Primers were designed from the sequence of a 4700 nucleotides cDNA fragment located in the 3'-end of the DWV genome. This fragment encodes a single open reading frame of 1565 amino acids showing similarity to viral RNA dependent RNA polymerase consensus motif. RT-PCR assays from DWV infected individual mite or bee produced a 395 nucleotide DNA fragment clearly identifiable by agarose gel electrophoresis. The signal in bees having deformed wings was significantly higher than in normal ones. A search for DWV in 40 colonies showed that DWV is broadly...
Tipo: Journal Article Palavras-chave: APIS MELLIFERA; VARROA DESTRUCTOR.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008c877c0ed&uri=/notices/prodinra1/2009/12/
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Prevalence and seasonal variations of six bee viruses in Apis mellifera L. and Varroa destructor mite populations in France Inra
Tentcheva, D.; Gauthier, L.; Zappulla, N.; Dainat, B.; Cousserans, F.; Colin, M. E.; Bergoin, M..
A survey of six bee viruses on a large geographic scale was undertaken by using seemingly healthy bee colonies and the PCR technique. Samples of adult bees and pupae were collected from 36 apiaries in the spring, summer, and autumn during 2002. Varroa destructor samples were collected at the end of summer following acaricide treatment. In adult bees, during the year deformed wing virus (DWV) was found at least once in 97% of the apiaries, sacbrood virus (SBV) was found in 86% of the apiaries, chronic bee paralysis virus (CBPV) was found in 28% of the apiaries, acute bee paralysis virus (ABPV) was found in 58% of the apiaries, black queen cell virus (BQCV) was found in 86% of the apiaries, and Kashmir bee virus (KBV) was found in 17% of the apiaries. For...
Tipo: Journal Article Palavras-chave: DEFORMED WING VIRUS; APIS MELLIFERA L.; VARROA DESTRUCTOR.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008cf3349ed&uri=/notices/prodinra1/2009/03/
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Viral load estimation in asymptomatic honey bee colonies using the quantitative RT-PCR technique Inra
Gauthier, L.; Tentcheva, D.; Tournaire, M.; Dainat, B.; Cousserans, F.; Colin, M. A.; Bergoin, A..
Honey bee (Apis mellifera L.) colonies are subjected to many persistent viral infections that do not exhibit clinical signs. The identification of criteria that could identify persistent or latent infections in bee colonies is a challenging task for field investigators and beekeepers. With this aim in view, we developed a molecular method to estimate the viral loads for six different RNA viruses in bee and mite individuals collected from seemingly healthy colonies (360 colonies). The data showed very large viral titres in some samples (> 10(9) copies per bee or mite). Discrepancies between adults and pupae viral RNA loads and, in several instances, significant seasonal variations among viruses were observed. The high titres of some RNA viruses recorded...
Tipo: Journal Article Palavras-chave: APIS MELLIFERA; VARROA DESTRUCTOR; QUANTITATIVE PCR; VIRAL LOAD.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20085337d0c4&uri=/notices/prodinra1/2009/12/
Registros recuperados: 7
Primeira ... 1 ... Última
 

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