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Análise da expressao gênica do VEGF (vascular Endothelial growth factor) em placentas bovinas de animais clonados, produzidos in vitro e in vivo, Pelo método de rt-pcr semiquantitativo Repositório Alice
LANDIM, JR, L. P.; FERREIRA, C. R.; PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; MIGLINO, M. A.; GARCIA, J. M..
O sucesso da produção de embriões por fecundação in vitro ou transferência nuclear depende de uma variedade de fatores, dos quais a formação de uma incompleta vascularização placentária representa uma das principais causas de perda gestacional precoce. Neste trabalho, avaliou-se a expressão relativa de Fator de Crescimento Endotélio-vascular (VEGF), um potente agente vasculogênico, em placentas de bovinos clonados por transferência nuclear (NT), produzidos por fecundação in vitro (FIV), ou por monta natural (MN). Verificou-se um decréscimo altamente significativo de expressão de VEGF nas placentas dos animais produzidos in vitro, quando comparados aos animais MN (1,32a; 0,21b; 0,31b, respectivamente para MN, FIV e NT, p<0,05; teste de Duncan), sugerindo...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Análise de expressão génica; Semiquantitativo; Placentas Bovinas; VEGF; Clone; RT-PCR.
Ano: 2006 URL: http://www.alice.cnptia.embrapa.br/handle/doc/256248
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Análise Dopplerfluxométrica e angiogênica de tumores mamários caninos Arq. Bras. Med. Vet. Zootec.
Navarro,D.M.; Silva,D.M.F.; Costa,F.S.; Wischral,A..
RESUMO Foi avaliado o comportamento de índices Doppler e a expressão de genes relacionados à neovascularização tumoral, visando caracterizar a vascularização das massas neoplásicas. Foram utilizadas 27 cadelas, com diagnóstico histopatológico de neoplasia mamária, sendo submetidas à avaliação Dopplerfluxométrica tumoral e à coleta de fragmentos neoplásicos para análise de expressão gênica de VEGF, FLT-1, FLK-1 e ATR1. Foram encontrados 22 tumores de origem epitelial (carcinomas) e cinco de origem mesenquimal (sarcomas). Observou-se correlação positiva entre o FLT-1 e as variáveis PS, PI e RI. O FLK-1 apresentou correlação igualmente positiva com os parâmetros PS e PI e uma tendência para RI, enquanto o VEGF retratou correlação positiva apenas com IP. O...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cães; Neoplasia; Doppler; ATR1; VEGF.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401099
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Angiogenesis and Tumor Progression Inhibition of Cyclooxygenase-2 Selective Inhibitor Celecoxib Associated with Poly (lactic-co-glycolic acid) in Tumor Cell Line Resistant to Chemotherapy International Journal of Morphology
Roa,Ignacio; Cantín,Mario; Vilos,Cristian; Rosas,Carlos; Lemus,David.
Although, antineoplastic therapies have now been developed reduction of tumor progression,itis necessarytofind new therapeutic alternatives to suppress angiogenesis.Thus celecoxib (Cx) has been used for its antiangiogenic action in combination with certain polymeric compounds such as poly (lactic co-glycolic acid) (PLGA) acid, which help to improve the bioavailability and avoid effects of long drug administrations. For this purpose we used a murine tumor modelinduced by mammary adenocarcinoma cells resistant to chemotherapy (TA3-MTXR). CX/PLGA inhibits the microvascular density, VEGF expression and cell proliferationinaddition to increased apoptosis (P <0.0001). Cx reduces tumor progression in a concentration of 1000 ppm associated with PLGA, reducing...
Tipo: Journal article Palavras-chave: Angiogenesis; Cancer; Celecoxib; PLGA; Apoptosis; VEGF; CellProliferation.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200055
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Atuação do Vegf na placenta de ratas diabéticas induzidas por aloxana MV&Z
S. Farias, Priscilla; dos S. Souza, Karine; T. Fioretto, Emerson; B. Aires, Marlúcia.
A diabetes mellitus gestacional ocorre em cerca de 7% de todas as gestações,resultando em mais de 200.000 casos/ano. Os distúrbios hiperglicêmicosassociados a essa condição levam a alterações da estrutura e função da placenta o que acarreta problemas na transferência de nutrientes entre mãe e feto. O objetivo do trabalho foi avaliar o perfil glicêmico, ganho de peso materno, e expressão de VEGF em placentas de ratas Wistar diabéticas. Foram utilizadas ratas no 8º dg para aplicação de aloxana ou solução salina. O peso corporal e a glicemia foram avaliados no 8º dg e 17º dg. No 17º dg os animais foram anestesiados e laparotomizados para coleta das placentas. Foi realizada análise histológica e imunocitoquímica para VEGF. A aplicação de aloxana foi capaz de...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diabetes; Gestação; Imunocitoquímica; VEGF; Placenta.
Ano: 2014 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/23719
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Development of VEGF-loaded PLGA matrices in association with mesenchymal stem cells for tissue engineering BJMBR
Rosa,A.R.; Steffens,D.; Santi,B.; Quintiliano,K.; Steffen,N.; Pilger,D.A.; Pranke,P..
The association of bioactive molecules, such as vascular endothelial growth factor (VEGF), with nanofibers facilitates their controlled release, which could contribute to cellular migration and differentiation in tissue regeneration. In this research, the influence of their incorporation on a polylactic-co-glycolic acid (PLGA) scaffold produced by electrospinning on cell adhesion and viability and cytotoxicity was carried out in three groups: 1) PLGA/BSA/VEGF; 2) PLGA/BSA, and 3) PLGA. Morphology, fiber diameter, contact angle, loading efficiency and controlled release of VEGF of the biomaterials, among others, were measured. The nanofibers showed smooth surfaces without beads and with interconnected pores. PLGA/BSA/VEGF showed the smallest water contact...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Emulsion electrospinning; VEGF; Stem cells; PLGA; Biomaterials.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900601
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Efectos de la restricción alimentaria sobre el desarrollo de los vasos sanguíneos placentarios en cabras InVet
Coniglio,MV; Merkis,CI; Diaz,T; Romanini,MC; Turiello,MP; Bozzo,AA; Cots,DS; Rolando,AN.
El objetivo fue estudiar los efectos de la restricción alimentaria sobre la angiogénesis placentaria en cabras Anglo nubian. Se utilizaron 19 placentas a término de tres grupos de estudios: control, restringido y restringido con monensina. Los cortes placentarios se procesaron con técnicas histológicas de rutina e inmunomarcaron con anticuerpo anti-VEGF y se cuantificó el área y perímetro de los vasos sanguíneos. El perímetro de los vasos de los grupos restringidos fue mayor estadísticamente con respecto al control. Mientras que el área de los mismos no mostró diferencias estadísticamente significativas en ninguno de los tres grupos. El VEGF se localizó en las células epiteliales trofoblásticas de los grupos control y restringido, además en éstos últimos...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Placenta; Angiogénesis; VEGF; Cabra.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982016000100009
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Effects of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) release from polylactide-poly (ethylene glycol)-polylactide (PELA) microcapsule-based scaffolds on bone BJMBR
Ren,Q.; Cai,M.; Zhang,K.; Ren,W.; Su,Z.; Yang,T.; Sun,T.; Wang,J..
Multiple growth factors can be administered to mimic the natural process of bone healing in bone tissue engineering. We investigated the effects of sequential release of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) from polylactide-poly (ethylene glycol)-polylactide (PELA) microcapsule-based scaffolds on bone regeneration. To improve the double emulsion/solvent evaporation technique, VEGF was encapsulated in PELA microcapsules, to which BMP-2 was attached. The scaffold (BMP-2/PELA/VEGF) was then fused to these microcapsules using the dichloromethane vapor method. The bioactivity of the released BMP-2 and VEGF was then quantified in rat mesenchymal stem cells (rMSCs). Immunoblotting analysis showed that BMP-2/PELA/VEG...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bone tissue engineering; BMP-2; VEGF; PELA; Scaffold.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200603
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Effects of oxysterols on cell viability, inflammatory cytokines, VEGF, and reactive oxygen species production on human retinal cells: cytoprotective effects and prevention of VEGF secretion by resveratrol Inra
Dugas, B.; Charbonnier, S.; Baarine, M.; Ragot, K.; Delmas, D.; Menetrier, F.; Lherminier, J.; Malvitte, L.; Khalfaoui, T.; Bron, A.; Creuzot Garcher, C.; Latruffe, N.; Lizard, G..
Background and aimsOxysterols are assumed to play important roles in age-related macular degeneration, a major cause of blindness. So we characterized the cytotoxic, oxidative, inflammatory, and angiogenic activities of oxysterols (7β-hydroxycholesterol (7β-OH), 7-ketocholesterol (7KC), 25-hydroxycholesterol (25-OH)) in human retinal ARPE-19 cells, and evaluated the protective effects of resveratrol (Rsv: 1 μM), a polyphenol from red wine.MethodsARPE-19 cells were treated with 7β-OH, 7KC, or 25-OH (5–40 μg/mL; 24–48 h) without or with Rsv. Cell viability was determined using trypan blue and the MTT assay. Cell death was characterized by electron microscopy and in situ detection of activated caspases with fluorochrome-labeled inhibitors of caspases....
Tipo: Journal Article Palavras-chave: CELLULES RÉTINIENNES; DEGENERESCENCE MACULAIRE; DMLA ARPE-19 CELLS; CASPASE-INDEPENDENT CELL DEATH; INFLAMMATORY CYTOKINES; OXYSTEROLS; PHOSPHOLIPIDOSIS; RESVERATROL; REACTIVE OXYGEN SPECIES; VEGF.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201135a1139e&uri=/notices/prodinra1/2011/06/
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Expression of VEGF and CD68 in the Placenta of Gestational Diabetic Mothers (Immunohistochemistry and Ultrastructural Study) International Journal of Morphology
Yavuz,D; Balsak,D; Ekinci,C; Tahaoglu,A. E; Togrul,C; Görük,N; Aktas,A; Karaman,E.
Placental angiogenesis, is essential for embryonic and fetal development. In this study, 18 gestational diabetes mellitus and 22 control pregnancies were included. Gestational diabetes mellitus (GDM) groups compared to the control group significantly higher values were detected (p<0.01). The following histological results were assessed; villous immaturity, chorangiosis, presence of, sncytial knots,mononuclear cell infiltration ischemia and fibrinoid necrosis. To evaluate and compare the placental histology of normal and GDM pregnancies. placentas of pregnant women with gestational diabetes also in terms of angiogenesis and macrophages and ultratructural revealed by examining the possible relationship between fetal complications were investigated.
Tipo: Journal article Palavras-chave: Gestational Diabetes (GDM); VEGF; CD68; Ultrastructure.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200018
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Identificação imuno-histoquímica de VEGF e IGF-1 em ovários de cadelas no anestro e estro Arq. Bras. Med. Vet. Zootec.
Almeida,M.V.D.; Castro,D.S.; Rial,L.C.; Lustosa,J.P.; Bicalho,A.L.F.; Ortega,H.H.; Salvetti,N.R.; Caliari,M.V.; Vieira,F.G.; Valle,G.R..
Com o objetivo de verificar a presença de VEGF e IGF-1 nos ovários de cadelas, foram realizadas análises imuno-histoquímicas do estroma cortical; teca e granulosa de folículos secundários, terciários e terciários pré-ovulatórios luteinizados; e ovócitos de folículos primários, secundários e terciários de ovários de cinco cadelas em anestro (Anest) e cinco em estro (Est). A identificação das fases do ciclo estral foi realizada por citologia vaginal associada a dosagem plasmática de progesterona. Os ovários foram submetidos a tratamento imuno-histoquímico para identificação de VEGF (anticorpo primário PU 360-UP, Biogenex, USA; diluição 1:30) e IGF-1 (anticorpo primário PabCa, Gro-Pep, Austrália; diluição 1:100). Determinou-se um índice de imunomarcação (IM),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cadela; Ovário; Ovócito; VEGF; IGF-1.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200334
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In ovo administration of human recombinant leptin shows dose dependent angiogenic effect on chicken chorioallantoic membrane Biol. Res.
Manjunathan,Reji; Ragunathan,Malathi.
BACKGROUND: Leptin, the cytokine produced by white adipose tissue is known to regulate food energy homeostasis through its hypothalamic receptor. In vitro studies have demonstrated that leptin plays a major role in angiogenesis through binding to the receptor Ob-R present on ECs by stimulating and initiating new capillary like structures from ECs. Various in vivo studies indicate that leptin has diverse effect on angiogenesis. A few reports have showed that leptin exerts pro angiogenic effects while some suggested that it has antiangiogenic potential. It is theoretically highly important to understand the effect of leptin on angiogenesis to use as a therapeutic molecule in various angiogenesis related pathological conditions. Chicken chorio allantoic...
Tipo: Journal article Palavras-chave: Angiogenesis; HRL; Human Recombinant Leptin; CAM; Chorioallantoic membrane; ECs; Endothelial cells; VEGF; Vascular endothelial growth factor; MMP; Matrix metalloproteinase; Blood vessels; CD34.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100029
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Kaempferol inhibited VEGF and PGF expression and in vitro angiogenesis of HRECs under diabetic-like environment BJMBR
Xu,X.H.; Zhao,C.; Peng,Q.; Xie,P.; Liu,Q.H..
Diabetic retinopathy (DR) is one of the common and specific microvascular complications of diabetes. This study aimed to investigate the anti-angiogenic effect of kaempferol and explore its underlying molecular mechanisms. The mRNA expression level of vascular endothelial growth factor (VEGF) and placenta growth factor (PGF) and the concentrations of secreted VEGF and PGF were measured by qTR-PCR and ELISA assay, respectively. Human retinal endothelial cells (HRECs) proliferation, migration, and sprouting were measured by CCK-8 and transwell, scratching wound, and tube formation assays, respectively. Protein levels were determined by western blot. High glucose (25 mM) increased the mRNA expression levels of VEGF and PGF as well as the concentrations of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diabetic retinopathy; Kaempferol; VEGF; PGF; Anti-angiogenic.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300605
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lncRNA CCAT1 promotes cell proliferation, migration, and invasion by down-regulation of miR-143 in FTC-133 thyroid carcinoma cell line BJMBR
Yang,Tianzheng; Zhai,Hongyan; Yan,Ruihong; Zhou,Zhenhu; Gao,Lei; Wang,Luqing.
Thyroid cancer is a common malignant tumor. Long non-coding RNA colon cancer-associated transcript 1 (lncRNA CCAT1) is highly expressed in many cancers; however, the molecular mechanism of CCAT1 in thyroid cancer remains unclear. Hence, this study aimed to investigate the effect of CCAT1 on human thyroid cancer cell line FTC-133. FTC-133 cells were transfected with CCAT1 expressing vector, CCAT1 shRNA, miR-143 mimic, and miR-143 inhibitor, respectively. After different treatments, cell viability, proliferation, migration, invasion, and apoptosis were measured. Moreover, the regulatory relationship of CCAT1 and miR-143, as well as miR-143 and VEGF were tested using dual-luciferase reporter assay. The relative expressions of CCAT1, miR-143, and VEGF were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thyroid cancer; CCAT1; MiR-143; VEGF; PI3K/AKT pathway; MAPK pathway.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600615
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Mechanism of hif-1α mediated hypoxia-induced permeability changes in bladder endothelial cells BJMBR
Liu,C.; Shui,C.L.; Wang,Q.; Luo,H.; Gu,C.G..
This study aimed to investigate the mechanism of hypoxia-inducible factor-1 alpha (HIF-1α) mediated hypoxia-induced permeability changes in bladder endothelial cells. Models of in vitro hypoxic cell culture of bladder cancer, bladder cancer cells with low HIF-1α expression and HIF-1α RNA interference (RNAi) expression vector were established. Western blot and reverse transcription polymerase chain reaction (RT-PCR) were used to detect the expression of HIF-1α and vascular endothelial growth factor (VEGF) in each group. Bladder cell permeability was determined. Results showed that protein and mRNA expression of HIF-1α and VEGF at 3 and 12 h of hypoxia were significantly higher than normal control (P<0.05), and peaked at 12 h. HIF-1α and VEGF expression...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bladder cancer; Hypoxia inducible factor-1α; VEGF; Cell permeability; YC-1.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200616
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Silencing HIF-1α reduces the adhesion and secretion functions of acute leukemia hBMSCs BJMBR
Dong-Feng,Zeng; Ting,Liu; Cheng,Chang; Xi,Zhang; Xue,Liang; Xing-Hua,Chen; Pei-Yan,Kong.
Hypoxia inducible factor-1α (HIF-1α) is an important transcription factor, which plays a critical role in the formation of solid tumor and its microenviroment. The objective of the present study was to evaluate the expression and function of HIF-1α in human leukemia bone marrow stromal cells (BMSCs) and to identify the downstream targets of HIF-1α. HIF-1α expression was detected at both the RNA and protein levels using real-time PCR and immunohistochemistry, respectively. Vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1α (SDF-1α) were detected in stromal cells by enzyme-linked immunosorbent assay. HIF-1α was blocked by constructing the lentiviral RNAi vector system and infecting the BMSCs. The Jurkat cell/BMSC co-cultured system...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HIF-1α; Human bone marrow stromal cells (hBMSCs); Leukemia; RNAi; SDF-1α; VEGF.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000004
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The Effects of Polymeric Zinc Propylen-Bis-Dithiocarbamate (Propineb) on Tooth Development in Rats International Journal of Morphology
Mahmut,Koparal; Faruk,Er; Engin,Deveci; Dilek,Yavuz.
Dithiocarbamate propinebs are organometal fungicides that are widely used for the control of diseases in plants. In this study, pregnant female rats received 400 ppm propineb concentrations in 5 ml distilled water for 16 days of gestation, and then infant rats were obtained by cesarean section. In the histological analysis on the frontal sections, the use of propineb was found effective on odontoblast cell hyperplasia, cell infiltration in the dental papilla, and degeneration in the mesenchymal cells of the outer enamel. The expression of MMP-2 (Matrix Metalloproteinase-2) and VEGF (Endothelial cell growth factor) in the connective tissue was evaluated by immunohistochemistry. The drinking water given to the mothers in propineb tooth bud, enamel and...
Tipo: Journal article Palavras-chave: Propineb; MMP2; Tooth buds; VEGF.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100014
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The role of KDR in intrauterine adhesions may involve the TGF-β1/Smads signaling pathway BJMBR
Chen,Jian Xia; Yi,Xi Juan; Gu,Pei Ling; Gao,Shan Xia.
The aim of this study was to investigate the role of kinase-insert domain-containing receptor (KDR) in intrauterine adhesions (IUA) and its mechanism. The Case group consisted of 92 patients diagnosed with IUA, and the Control group included 86 patients with uterine septum who had normal endometrium verified with an uteroscope. In addition, 50 rats were randomly assigned into Control, Sham, Model, NC-siRNA, and KDR-siRNA groups. Rats in the Model, NC-siRNA, and KDR-siRNA groups were induced by uterine curettage and lipopolysaccharide (LPS) treatment to establish the IUA model. Then, immunohistochemistry was applied for detection of VEGF and KDR expression, HE staining was used for observation of the endometrial morphology and gland counting, Masson...
Tipo: Info:eu-repo/semantics/article Palavras-chave: KDR; Intrauterine adhesions; TGF-β1/Smads; MMP9; VEGF.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000607
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Ventilation-induced changes correlate to pulmonary vascular response and VEGF, VEGFR-1/2, and eNOS expression in the rat model of postnatal hypoxia BJMBR
Figueira,R.L.; Gonçalves,F.L.; Prado,A.R.; Ribeiro,M.C.; Costa,K.M.; Silva,O. Castro e; Sbragia,L..
Neonatal asphyxia occurs due to reduction in oxygen supply to vital organs in the newborn. Rapid restoration of oxygen to the lungs after a long period of asphyxia can cause lung injury and decline of respiratory function, which result from the activity of molecules that induce vascular changes in the lung such as nitric oxide (NO) and vascular endothelial growth factors (VEGF). In this study, we evaluated the pulmonary and vascular morphometry of rats submitted to the model of neonatal asphyxia and mechanical ventilation, their expression of pulmonary VEGF, VEGF receptors (VEGFR-1/VEGFR-2), and endothelial NO synthase (eNOS). Neonate Sprague-Dawley rats (CEUA #043/2011) were divided into four groups (n=8 each): control (C), control submitted to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neonatal hypoxia; Mechanical ventilation; VEGF; VEGF receptors; ENOS enzyme.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001100608
Registros recuperados: 18
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