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Registros recuperados: 90
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A retrospective PCR investigation of avian Orthoreovirus, chicken infectious anemia and fowl Aviadenovirus genomes contamination in commercial poultry vaccines in Brazil Arq. Bras. Med. Vet. Zootec.
Barrios,P.R.; Marin,S.Y.; Rios,R.L.; Pereira,C.G.; Resende,M.; Resende,J.S.; Martins,N.R.S..
Vacinas avícolas vivas comerciais produzidas entre 1991 e 2005 foram examinadas para a presença de genomas dos vírus da anemia infecciosa das galinhas (Gyrovirus CAV), da hepatite por corpúsculo de inclusão (Aviadenovirus FAdV) e da artrite viral/síndrome da má absorção (Orthoreovirus aviário ARV). Vinte e seis partidas de vacinas vivas liofilizadas de oito fabricantes com lacre original foram examinadas. As extrações de DNA e PCR de CAV e FAdV, e de RNA e RT-PCR para ARV, foram descritas previamente. Contaminações triplas de ARV, CAV e FAdV foram detectadas em vacinas de mesmo fabricante, produzidas em 1991 e 1992 contra a doença de Newcastle (DN), e para a encefalomielite aviária, produzida em 1994. ARV e CAV em co-infecção foram encontrados em vacinas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken; Vaccine; Avian Orthoreovirus; Aviadenovirus; Gyrovirus.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000100035
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A single strain of Mycobacterium bovis bacillus Calmette-Guérin (BCG) grown in two different media evokes distinct humoral immune responses in mice BJMBR
Petricevich,V.L.; Ueda,C.; Alves,R.C.B.; da Silva,M.A.; Moreno,C.; Melo,A.R.; Dias da Silva,W..
Two attenuated bacillus Calmette-Guérin (BCG) preparations derived from the same Moreau strain, Copenhagen but grown in Sauton medium containing starch and bacto-peptone (onco BCG, O-BCG), or asparagine (intradermal BCG, ID-BCG), exhibited indistinguishable DNA sequences and bacterial morphology. The number of viable bacilli recovered from spleen, liver and lungs was approximately the same in mice inoculated with the vaccines and was similarly reduced (over 90%) in mice previously immunized with either BCG vaccine. The humoral immune response evoked by the vaccines was, however, distinct. Spleen cell proliferation accompanying the growth of bacilli in tissue was significantly higher in mice inoculated with O-BCG. These cells proliferated in vitro upon...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus Calmette-Guérin; BCG; Tuberculosis; Mycobacterium bovis; Vaccine.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100010
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Adaptation de la méthode peptide-spot à la recherche de sites, de la protéine RNA2 du nodavirus, reconnus par différents sérums de loup (Dicentrarchus labrax). ArchiMer
Coeurdacier, Jean-luc.
Spot-Synthesis technique was applied to study protein frame or to develop antibodies with specific characteristics for use in diagnostic or research on human or mammals. In this work this technique was used to find sites on RNA2 protein of Nodavirus which are immunogenic for sea bass (Dicentrarchus labrax). It aimed to achieve synthetic peptide vaccine production against this virus. One hundred and nine overlapping peptides, of 15 amino acids frameshifted by three residues, representing the complete RNA2 protein sequence of Sb1 and Sb2 nodavirus were synthesized on a cellulose membrane by the Spot-Technique. The set of membrane bound peptides was probed by incubation with sea-bass or rabbit serum. Anti sea bass or anti rabbit alkaline phosphatase...
Tipo: Text Palavras-chave: Peptides synthétiques; Vaccin; Peptid-spot; Séquence immunogène; Dicentrarchus labrax; Synthetic peptides; Vaccine; Immunogenicity; Dicentrarchus labrax...
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00157/26810/24919.pdf
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Alzheimer's disease: is a vaccine possible? BJMBR
Alves,R.P.S.; Yang,M.J.; Batista,M.T.; Ferreira,L.C.S..
The cause of Alzheimer's disease is still unknown, but the disease is distinctively characterized by the accumulation of β-amyloid plaques and neurofibrillary tangles in the brain. These features have become the primary focus of much of the research looking for new treatments for the disease, including immunotherapy and vaccines targeting β-amyloid in the brain. Adverse effects observed in a clinical trial based on the β-amyloid protein were attributed to the presence of the target antigen and emphasized the relevance of finding safer antigen candidates for active immunization. For this kind of approach, different vaccine formulations using DNA, peptide, and heterologous prime-boost immunization regimens have been proposed. Promising results are expected...
Palavras-chave: Alzheimer's disease; Β-amyloid; Vaccine; Active immunization.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600438
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Antibodies against rabies virus in dogs with and without history of vaccination in Santa Maria - RS - Brazil Ciência Rural
Fernandes,Karina Gonzalez; Martins,Mathias; Amaral,Bruna Portolan; Cargnelutti,Juliana Felipetto; Weiblen,Rudi; Flores,Eduardo Furtado.
ABSTRACT: The present study investigated the frequency and magnitude of neutralizing antibodies to rabies virus (RABV) in dogs with and without historic of vaccination in Santa Maria/RS. Group A included serum samples from 440 dogs with recent historic of vaccination against rabies, obtained during the 2015 rabies vaccination campaign. Group B included 300 serum samples from dogs submitted to the Veterinary Hospital of the Universidade Federal de Santa Maria in 2015, whose historic of rabies vaccination was unknown. Serum samples were submitted to the rapid fluorescent focus inhibition test (RFFIT) to detect neutralizing antibodies against RABV. In group A, 70.6% (310/440) of the samples had neutralizing antibody titers ≥0.5 international units per...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine; Immunity; RABV; Serological response; Vaccine.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001100452
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Assessment of immunity against avian colibacillosis induced by an aroA mutant containing increased serum survival gene in broilers BJM
Salehi,Taghi Zahraei; Tabatabaei,Saeid; Karimi,Vahid; Fasaei,Bahar Nayeri; Derakhshandeh,Abdollah; Jahromi,Omid Ali Nekoui.
Colibacillosis is an important disease in the poultry industry which causes serious economic damages. As it is suggested that vaccination is one of the means to control colibacillosis, we tried to investigate the vaccine potential of a ΔaroA derivative of an O78:K80 avian pathogenic Escherichia coli containing increased serum survival gene. 490 chicks were selected as follows: For assessment of virulence of ΔaroA mutant, 30 chicks were divided into three groups and injected with 0.5ml of PBS or bacterial suspension containing either10(7)colony forming units (CFU) of mutant or parent strains via subcutaneous route. Macroscopic lesions and mortality rate were recorded in different groups during the week after challenge. For assessment of safety and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chickens; Avian pathogenic Escherichia coli; ΔaroA mutant; Vaccine.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100043
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Avaliação genética das vacinas contra a brucelose bovina comercializadas no Brasil. Repositório Alice
BASTOS, R.; SOARES, C. O.; ELISEI, C.; MUNHOZ, A. L. R.; BEZERRA, N. L.; CAITANO, M. A. B.; ROSINHA, G. M. S..
Objetivou-se com este estudo caracterizar genotipicamente as amostras vacinais B19 e RB51 comercializadas no Brasil.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Brucella abortus; Brucellosis; Vaccine; B19; RB51; Genotyping; Genetic quality.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/937237
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Avaliação hematológica de ovinos imunizados com glicoproteina instestinal de Haemonchus contortus. Repositório Alice
MATOS, A. F. M.; SANTOS, T. L. B. A.; FONTENELE, J. A.; SIEBRA, P. S. C.; ALBUQUERQUE, F. H. M. A. R. de.; TEIXEIRA, M..
2014
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Raça Santa Inês; Nematóide; Helminto gastrintestinal; Hematologia; Vacina; Parasitismo; Haemonchus contortus; Ovino; Imunização; Vaccine; Sheep; Parasitism; Nematoda; Hematology; Immunization.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1009946
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Can vaccines against canine visceral leishmaniasis interfere with the serological diagnostics recommended by the Brazilian Ministry of Health? Ciência Rural
Campos,Monique Paiva de; Luca,Paula Mello De; Renzetti,Alinne Rangel dos Santos; Souza,Sara Maria Marques de; Mendes Júnior,Artur Augusto Velho; Barros,Renata Simões; Figueiredo,Fabiano Borges.
ABSTRACT: The objective of the current research was to assess seroconversion in dogs immunized with Leishmune® and Leish Tec® vaccines using rapid chromatographic immunoassay DPP® (Dual Path Platform) (DPP CVL) and enzyme immunoassay (EIE) up to one year after the vaccination protocol. The study sample comprised 28 dogs divided into two groups, each group immunized with an anti-CVL vaccine and clinically monitored for one year through clinical evaluation and laboratory tests. 22 (78.5%) dog were monitored. During the evaluation time (T1-30 days, T2-6 months, and T3-1 year after vaccination) the results for all dogs were negative for CVL, except for one animal vaccinated with Leish tec® that seroconverted in the DPP CVL test at T2. Subsequent examinations...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine visceral leishmaniasis; Vaccine; Diagnosis; Seroconversion.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000400503
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Candidacy of liver stage antigen-1 for Plasmodium falciparum vaccine development OAK
Taylor-Robinson, Andrew W.; Heal, Karen G..
Malaria is the world's most important parasitic disease, imposing a massive health burden on people living in the tropics, often in the poorest countries. The vast majority of deaths in humans from malaria are caused by one species of the hemoprotozoan, Plasmodium falciparum., against which effective control measures are urgently needed. The global situation has deteriorated in recent times due to increased resistance of the anopheline mosquitoes that transmit P. falciparum to insecticides, and of the parasites themselves to drug therapy. An efficacious and cost-effective vaccine against this parasite is considered a public health priority. A vaccine that targets pre-erythrocytic parasites in the liver could potentially prevent clinical disease by blocking...
Palavras-chave: Malaria; Plasmodium falciparum; Liver stage; Vaccine; Cellular immunity.
Ano: 2001 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/133
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Carriage prevalence of Neisseria meningitidis in the Americas in the 21st century: a systematic review BJID
Santos-Neto,José Francisco; Ferreira,Viviane Matos; Feitosa,Caroline Alves; Martinez-Silveira,Martha Silvia; Campos,Leila Carvalho.
Abstract Neisseria meningitidis is a bacterium that colonizes the human nasopharynx and is transmitted by respiratory droplets from asymptomatic or symptomatic carriers. Occasionally, the pathogen invades the mucosa and enters the bloodstream, causing invasive meningococcal disease, a life-threatening infection. While meningococcal colonization is the first step in the development of invasive disease, the risk factors that predict progression from asymptomatic to symptomatic status are not well-known. The present report aimed to describe the prevalence of N. meningitidis carriers throughout the Americas, emphasizing the risk factors associated with carrier status, as well as the most prevalent serogroups in each studied population. We conducted a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neisseria meningitidis; Carrier; America; Epidemiology; Vaccine.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000400254
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Cepas atenuadas de Babesia bovis e Babesia bigemina e de Anaplasma centrale como imunogenos no controle da Tristeza parasitaria bovina. Repositório Alice
SACCO, A. M. S.; KESSLER, R. H.; MADRUGA, C. R..
Neste trabalho foram avaliadas cepas atenuadas de Babesia bovis e B. bigemina e Anaplasma centrale como imunogenos a serem utilizados no controle da Tristeza Parasitaria Bovina. O processo de imunizacao demonstrou ser inocuo. imunogenico e eficiente, pelo menos no que diz respeito as babesias, pois protegeu os animais vacinados frente ao desafio com cepas heterologas virulentas a campo, que provocou doenca clinica e morte nos animais do grupo controle. O desafio a campo pelo Anaplasma marginale nao se mostrou muito virulento ou patogenico, nao sendo possivel concluir que a imunizacao com A.centrale proteja os animais contra anaplasmose.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Anaplasmosse; Tickborne disease; Vaccine; Babesia Bigemina; Babesia Bovis; Babesiose; Bovino; Imunologia; Sanidade Animal; Tristeza Parasitaria; Vacina; Anaplasma centrale; Anaplasmosis; Animal health; Babesiosis; Cattle; Immunology.
Ano: 2001 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/324413
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Clinical and bacteriological characteristics of invasive pneumococcal disease after pneumococcal 10-valent conjugate vaccine implementation in Salvador, Brazil BJID
Leite,Carolina Regis; Azevedo,Jailton; Galvão,Vivian Santos; Moreno-Carvalho,Otávio; Reis,Joice Neves; Nascimento-Carvalho,Cristiana.
Abstract Invasive pneumococcal disease is a relevant public health problem in Brazil, especially among children and the elderly. In July/2010 a 10-valent pneumococcal conjugate vaccine was introduced to the immunization schedule of Brazilian children under two years of age. Between July/2010 and December/2013 we conducted a case-series study on invasive pneumococcal disease in Salvador, Brazil to describe the clinical and bacteriological profile of invasive pneumococcal disease cases during the post-implementation period. Eighty-two cases were eligible. Mean age was 31 years (interquartile range, 3–42); 17.1% and 30.5% were under 2 years and 5 years, respectively. Pneumococcal meningitis (n = 64, 78.1%), bacteraemic pneumococcal pneumonia (n = 12, 14.6%)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptococcus pneumoniae; Vaccine; Epidemiology.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000100056
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Comparative sequence analysis of the P-, M- and L-coding region of the measles virus CAM-70 live attenuated vaccine strain BJMBR
Santos,P.R.; Azevedo,M.L.B.; Borges,M.B.J.; Freire,M.S.; Nascimento,J.P.; Moraes,M.T.B..
Measles virus is a highly contagious agent which causes a major health problem in developing countries. The viral genomic RNA is single-stranded, nonsegmented and of negative polarity. Many live attenuated vaccines for measles virus have been developed using either the prototype Edmonston strain or other locally isolated measles strains. Despite the diverse geographic origins of the vaccine viruses and the different attenuation methods used, there was remarkable sequence similarity of H, F and N genes among all vaccine strains. CAM-70 is a Japanese measles attenuated vaccine strain widely used in Brazilian children and produced by Bio-Manguinhos since 1982. Previous studies have characterized this vaccine biologically and genomically. Nevertheless, only...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Measles virus; Vaccine; Nucleotide sequence; RNA sequencing.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100005
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Construction of Recombinant Feline Herpesvirus Type 1 Expressing Toxoplasma gondii SRS1 OAK
Mishima, Masayuki; Xuan, Xuenan; Takeiri, Akira; Makala, Levi H. C; Igarashi, Ikuo; Fujisaki, Kozo; Nagasawa, Hideyuki; Mikami, Takeshi.
Palavras-chave: Toxoplasma; Vaccine; SRS1; FHV.
Ano: 2000 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/343
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Control of Newcastle Disease in Village Chickens AgEcon
Centre for International Economics.
Between 1983 and 1992, the Australian Centre for International Agricultural Research (ACIAR) invested A$3 million in research to find a vaccine that could provide protection from Newcastle disease in chickens and be applied in village environments in developing countries. A further $160 000 was invested in follow up projects which ended in 1996. Village chickens often provide the only source of protein to poor villagers living in remote areas and Newcastle disease frequently devastates unvaccinated village flocks. The ACIAR-sponsored research was highly successful in developing a heat resistant vaccine (HRV4) which could be readily used in the field by coating it onto chicken feed. The vaccine was commercialised by an Australian company which subsequently...
Tipo: Book Palavras-chave: Newcastle disease; Chickens; Poultry; Vaccine; Malaysia; Vietnam; ACIAR; Australia; Developing country; Village; Africa; Farm Management; Food Consumption/Nutrition/Food Safety; Food Security and Poverty; International Development; Livestock Production/Industries; Research and Development/Tech Change/Emerging Technologies; Research Methods/ Statistical Methods.
Ano: 1998 URL: http://purl.umn.edu/47190
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Development and evaluation of a novel subunit vaccine for Mycoplasma gallisepticum Arq. Bras. Med. Vet. Zootec.
Moura,L.; Dohms,J.; Almeida,J.M.; Ferreira,P.S.; Biffi,C.P.; Backes,R.G..
Adhesion proteins from Mycoplasma gallisepticum (MG) encoded by cytadhesion genes mgc1 and mgc2 were cloned into plasmid vectors and transformed into E. coli. Seventeen groups of specific-pathogen free (SPF), birds at four weeks of age were used to inoculate these two proteins (MGC1 and MGC2) mixed into an oil emulsion creating a novel MG vaccine. Six different protein concentrations (50, 100, 200, 400, 800, and 1000µg/bird) were tested with two equal concentration doses at four and seven weeks of age. In addition, many control groups were needed such as bacterin, membrane, no vaccine or challenge, oil emulsion alone, and no vaccine but challenged. Three weeks following the second vaccination, 50% of the birds in each treatment group were challenged with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Evaluation; Development; Mycoplasmas; Poultry; Vaccine.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000600024
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Double-dose hepatitis B vaccination in cirrhotic patients on a liver transplant waiting list BJID
Bonazzi,Patricia R.; Bacchella,Telesforo; Freitas,Angela C.; Osaki,Karina T.; Lopes,Marta H.; Freire,Maristela P.; Machado,Marcel C.C.; Abdala,Edson.
Development of immunity to hepatitis B virus in cirrhotic patients waiting for liver transplantation is highly desirable. Though a double-dose regimen is available, little is know about its effectiveness. We examined the efficacy of double-dose hepatitis B virus vaccination in cirrhotic patients waiting for liver transplantation. We studied 43 patients who were waiting for liver transplantation. They were vaccinated with three doses of 40 mg hepatitis B vaccine at 0, 1 and 6 months; the normal dose is 20 mg. Efficacy was measured based on seroconversion of anti-HBs. Global response to the primary vaccination scheme was 67.5% (29 patients). Forty-one per cent of responders had anti-HBs titers above 1,000 IU/mL. No factors were associated with response,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vaccine; Cirrhotic; Hepatitis B; Liver transplant.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400009
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Effect of Oligomannose-Coated Liposome-Based Vaccine on Rodent Babesiosis OAK
Ishigame, T; Takabatake, N; Iseki, H; Ota, N; Koyama, A; Igarashi, I; Nishikawa, Y; Ikehara, Y; Kojima, N; Yokoyama, N; 五十嵐, 郁男; 西川, 義文; 横山, 直明.
Oligomannose-coated liposome (OML)-based vaccines have been reported to induce Th1-based immunity against entrapped antigens in immunized animals and to show protective effects against several protozoal diseases. In the present study, we produced a recombinant Babesia rodhaini ribosomal phosphoprotein P0 (rBrP0) that showed immunological cross reactivity with another rodent Babesia parasite, B. microti. We evaluated the efficacy of vaccination with OML-entrapped rBrP0 on B. rodhaini and B. microti infections in mice. Prior immunization with the OML-based rBrP0 vaccine, or with a Freund’s adjuvant-based rBrP0 vaccine, failed to demonstrate any protective effect against lethal infection with B. rodhaini, but the OML-based vaccine did induce protective...
Palavras-chave: Oligomannose-coated liposomes (OML); Vaccine; Babesiosis; Babesia rodhaini; Babesia microti; P0.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2383
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Effect of vitamin E levels on the cell-mediated immunity of broilers vaccinated against coccidiosis Rev. Bras. Ciênc. Avic.
Silva,ICM da; Ribeiro,AML; Canal,CW; Vieira,MM; Pinheiro,CC; Gonçalves,T; de Moraes,ML; Ledur,VS.
Studies on the relationships between animal nutrition and immunity have sought reliable methodologies to measure responses. Cell-mediated immune response is similarly studied in humans. The cutaneous basophil hypersensitivity test (CBH) is one of the methods to measure that response and consists in the infiltration of inflammatory cells, particularly of lymphocytes and basophils, as result of the application of substances capable of inducing cell proliferation in determined sites, such as wings, wattle, and interdigital space in birds. CBH is considered a simple and fast method and can be applied in birds of different ages. In immunocompetence studies with poultry, phytohemagglutinin-P (PHA-P) is a commonly used substance, despite the variability of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broiler; Coccidiosis; Cell-mediated immune response; Newcastle disease; Vaccine; Vitamin E.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2011000100008
Registros recuperados: 90
Primeira ... 12345 ... Última
 

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