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Registros recuperados: 89
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A bovine herpesvirus 5 recombinant defective in the thymidine kinase (TK) gene and a double mutant lacking TK and the glycoprotein E gene are fully attenuated for rabbits BJMBR
Silva,S.C.; Brum,M.C.S.; Weiblen,R.; Flores,E.F.; Chowdhury,S.I..
Bovine herpesvirus 5 (BoHV-5), the agent of herpetic meningoencephalitis in cattle, is an important pathogen of cattle in South America and several efforts have been made to produce safer and more effective vaccines. In the present study, we investigated in rabbits the virulence of three recombinant viruses constructed from a neurovirulent Brazilian BoHV-5 strain (SV507/99). The recombinants are defective in glycoprotein E (BoHV-5gEΔ), thymidine kinase (BoHV-5TKΔ) and both proteins (BoHV-5gEΔTKΔ). Rabbits inoculated with the parental virus (N = 8) developed neurological disease and died or were euthanized in extremis between days 7 and 13 post-infection (pi). Infectivity was detected in several areas of their brains. Three of 8 rabbits inoculated with the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: BoHV-5; GE and TK deletion mutants; Pathogenesis; Virulence; Vaccine candidate.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200005
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A fast and simple assay to quantify bacterial leukotoxin activity Electron. J. Biotechnol.
Oppermann,Tobias; Schwarz,Stefan; Busse,Nadine; Czermak,Peter.
Background: Mannheimia haemolytica is the primary bacterial pathogen in causing bovine respiratory disease with tremendous annual losses in the cattle industry. The leukotoxin from M. haemolytica is the predominant virulence factor. Several leukotoxin activity assays are available but not standardized regarding sample preparation and cell line. Furthermore, these assays suffer from a high standard error, a prolonged time consumption and often complex sample pretreatments, which is important from the bioprocess engineering point of view. Results: Within this study, an activity assay based on the continuous cell line BL3.1 combined with a commercial available adenosine triphosphate viability assay kit was established. The leukotoxin activity was found to be...
Tipo: Journal article Palavras-chave: ATP assay; Bacterial pathogen; Bovine respiratory disease; Cattle industry; Critical parameters; Lipopolysaccharides; Mannheimia haemolytica; Microbial biotechnology; Polymyxin B; Quantification; Virulence.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600006
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A preliminary survey of M. hyopneumoniae virulence factors based on comparative genomic analysis Genet. Mol. Biol.
Ferreira,Henrique Bunselmeyer; Castro,Luiza Amaral de.
Mycoplasma hyopneumoniae is the etiological agent of porcine enzootic pneumonia (PEP), a major problem for the pig industry. The mechanisms of M. hyopneumoniae pathogenicity allow to predict the existence of several classes of virulence factors, whose study has been essentially restricted to the characterization of adhesion-related and major antigenic proteins. The now available complete sequences of the genomes of two pathogenic and one non-pathogenic strain of M. hyopneumoniae allowed to use a comparative genomics approach to putatively identify virulence genes. In this preliminary survey, we were able to identify 118 CDSs encoding putative virulence factors, based on specific criteria ranging from predicted cell surface location or variation between...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Porcine enzootic pneumoniae; Virulence; Host-pathogen interaction; Antigen; Adhesin.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200012
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An Overview on Marek’s Disease Virus Evolution and Evidence for Increased Virulence in Brazil Rev. Bras. Ciênc. Avic.
Torres,ACD; Marin,SY; Costa,CS; Martins,NRS.
ABSTRACT Marek’s disease virus (MDV) has been shown to be evolving to higher virulence. One of the genetic sites involved in virulence which enables such characterization is the 339-amino acid Meq protein encoding gene (meq). The reemergence of clinical Marek’s disease (MD) in vaccinated flocks can be associated to changes in meq. Our studies have shown the presence of very virulent MDV strains in the Brazilian industrial and free-range poultry. We present an overview of MD increasing severity and indicate the necessity of using phylogenetic tools for best accompanying MDV evolution.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gallid herpesvirus 2; Marek’s disease virus; Gallus gallus domesticus; Meq gene; Virulence.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100325
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Antracnose (Colletotrichum graminicola): resistência genética em sorgo e diversidade fenotípica no patógeno. Repositório Alice
CASELA, C.R.; FERREIRA, A.S.; SANTOS, F.G..
1998
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Sorgo; Antracnose; Resistencia; Genetica; Variabilidade; Virulencia; Sorghum bicolor; Colletotrichum graminicola; Resistance; Genetic; Variability; Virulence.
Ano: 1998 URL: http://www.alice.cnptia.embrapa.br/handle/doc/480589
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Associações de virulência de Colletotrichum graminicola a resistência genética em sorgo. Repositório Alice
CASELA, C.R.; FERREIRA, A.S.; SANTOS, F.G..
x
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Sorgo; Antracnose; Variabilidade; Analise; Virulencia; Resistencia; Sorghum bicolor; Colletotrichum graminicola; Disease; Anthracnose; Variability; Analysis; Virulence; Resistance.
Ano: 1997 URL: http://www.alice.cnptia.embrapa.br/handle/doc/477171
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Associações de virulência em Colletotrichum graminicola agente causal da antracnose em sorgo. Repositório Alice
CASELA, C.R.; FERREIRA, A.S..
Analisou-se, por dois anos, a estrutura de virulencia na populacao de Colletotrichum graminicola, agente causal da antracnose do sorgo, atraves das frequencias esperadas e observadas de virulencia a todos os pares possiveis de uma serie diferencial formada por dez cultivares de sorgo. Calculou-se tambem, para cada par, um coeficiente de associacao de patogenicidade (CAP) e um coeficiente de associacao de virulencia (CAV). Associacao negativa de virulencia foi observada apenas em relacao a duas cultivares Tx378 e SC748-5. Tal fato e indicativo de que individuos na populacao de patogeno com virulencia a estes genotipos possuem uma menor agressividade. Um grande numero de pares analisados apresentou um alto valor de CAP e um baixo valor de CAV. Combinacoes de...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Sorgo; Antracnose; Variabilidade; Analise; Virulencia; Resistencia; Sorghum bicolor; Colletotrichum graminicola; Anthracnose; Variability; Analysis; Virulence; Resistance.
Ano: 1995 URL: http://www.alice.cnptia.embrapa.br/handle/doc/477179
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Avaliação da virulência de isolados de Corynebacterium pseudotuberculosis em camundongos BALB/c Infoteca-e
ROSINHA, G. M. S.; MELO, P. R. de; SANTOS, L. R. dos; CARVALHO, C. E. G.; ALVARENGA, L. C. O.; ANDRADE, G. B.; SOARES, C. O..
Corynebacterium pseudotuberculosis é uma bactéria Gram-positiva, agente etiológico da linfadenite caseosa (LC) em pequenos ruminantes e causadora de piogranulomas, linfangite ulcerativa, mastite e dermatite ulcerativa em bovinos, que são doenças de grande relevância médica e veterinária. Esta doença é de difícil controle e a vacinação é a maneira mais eficiente de preveni-la nos rebanhos. Objetivou-se neste estudo selecionar cepas virulentas de C. pseudotuberculosis, a partir de cinco isolados obtidos de caprinos e ovinos, para uso em estudos de desafio em testes de novas vacinas contra linfadenite caseosa em camundongos. Primeiramente, os isolados foram caracterizados bioquimicamente com o kit API Coryne (APIbioMerieux, Inc., La Balme les Grottes, France)...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Corynebacterium Pseudotuberculosis; Histopatologia; Linfadenite Caseosa; Patogenicidade; Caseous lymphadenitis; Virulence; Histopathology.
Ano: 2018 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1106896
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Biological and molecular characterization of two Anticarsia gemmatalis multiple nucleopolyhedrovirus clones exhibiting contrasting virulence. Repositório Alice
FERREIRA, B. C.; MELO, F. L.; SILVA, A. M. R.; SANCHES, M. M.; MOSCARDI, F.; RIBEIRO, B. M.; SOUZA, M. L. de.
bitstream/item/196817/1/1-s2.0-S0022201118301769-main.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: AgMNPV; In vitro clones; Pyrosequencing; Anticarsia Gemmatalis; Baculovirus; Virulence.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1108589
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Bioprotective Effect of Lactococcus piscium CNCM I-4031 Against Listeria monocytogenes Growth and Virulence ArchiMer
Saraoui, Taous; Leroi, Francoise; Chevalier, Frederique; Cappelier, Jean-michel; Passerini, Delphine; Pilet, Marie-france.
Listeria monocytogenes is a Gram-positive pathogen occurring in many refrigerated ready-to-eat foods. It is responsible for foodborne listeriosis, a rare but severe disease with a high mortality rate (20–30%). Lactococcus piscium CNCM I-4031 has the capacity to prevent the growth of L. monocytogenes in contaminated peeled and cooked shrimp and in a chemically defined medium using a cell-to-cell contact-dependent mechanism. To characterize this inhibition further, the effect of L. piscium was tested on a collection of 42 L. monocytogenes strains. All strains were inhibited but had different sensitivities. The effect of the initial concentration of the protective and the target bacteria revealed that the inhibition always occurred when L. piscium had reached...
Tipo: Text Palavras-chave: Biopreservation; Lactococcus piscium; Listeria monocytogenes; Co-culture; Cell ratio; Scanning electron microscopy; Virulence.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00449/56065/57588.pdf
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Biotyping and genotypic diversity among oral Candida albicans strains from caries-free and caries-active healthy children BJM
Mardegan,Rita de Cássia; Klein,Marlise Inêz; Golvea,Magda Baglione; Rodrigues,Janaina Aparecida Oliveira; Gonçalves,Reginaldo Bruno; Höfling,José Francisco.
Candida albicans oral strains collected from caries-free and caries-active healthy children ranging from 24 to 36 months old, were studied. The aim of the study was to determine proteinase and phospholipase activities produced by Candida albicans in the two groups and to determine the phenotypic diversity of these enzymes based on genetic polymorphism using the AP-PCR method. Strains identified by morphological and fermentation tests as C. albicans were grown in proteinase and phospholipase agar media at 37ºC for 7 and 4 days, respectively. After the incubation period, the enzyme activity of the proteinase and phospholipase positive strains was measured. All strains were subjected to AP-PCR, using the arbitrary primer AP-3. The enzymatic analysis showed no...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Genotyping; Proteinase; Phospholipase; Virulence.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100005
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Candida albicans morphologies revealed by scanning electron microscopy analysis BJM
Staniszewska,M.; Bondaryk,M.; Swoboda-Kopec,E.; Siennicka,K.; Sygitowicz,G.; Kurzatkowski,W..
Scanning electron microscope (SEM) observations were used to analyze particular morphologies of Candida albicans clinical isolate (strain 82) and mutants defective in hyphae-promoting genes EFG1 (strain HLC52) and/ or CPH1 (strains HLC54 and Can16). Transcription factors Efg1 and Cph1 play role in regulating filamentation and adhesion of C. albicans' morphologies. Comparative analysis of such mutants and clinical isolate showed that Efg1 is required for human serum-induced cell growth and morphological switching. In the study, distinct differences between ultrastructural patterns of clinical strain's and null mutants' morphologies were observed (spherical vs tube-like blastoconidia, or solid and fragile constricted septa vs only the latter observed in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Pleomorphism; Ultrastructure; Virulence.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300023
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Carbapenem-resistant Pseudomonas aeruginosa: association with virulence genes and biofilm formation BJM
Gonçalves,Iara Rossi; Dantas,Raquel Cristina Cavalcanti; Ferreira,Melina Lorraine; Batistão,Deivid William da Fonseca; Gontijo-Filho,Paulo Pinto; Ribas,Rosineide Marques.
Abstract Pseudomonas aeruginosa is an opportunistic pathogen that causes frequently nosocomial infections, currently becoming more difficult to treat due to the various resistance mechanisms and different virulence factors. The purpose of this study was to determine the risk factors independently associated with the development of bacteremia by carbapenem-resistant P. aeruginosa, the frequency of virulence genes in metallo-β-lactamases producers and to evaluate their ability to produce biofilm. We conducted a case–control study in the Uberlândia Federal University – Hospital Clinic, Brazil. Polymerase Chain Reaction was performed for metallo-β-lactamases and virulence genes. Adhesion and biofilm assays were done by quantitative tests. Among the 157 strains...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Resistance; Metallo-β-lactamases; Virulence; Biofilm.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200211
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Carum copticum and Thymus vulgaris oils inhibit virulence in Trichophyton rubrum and Aspergillus spp BJM
Khan,Mohd Sajjad Ahmad; Ahmad,Iqbal; Cameotra,Swaranjit Singh.
Emergence of drug-resistant strains has demanded for alternative means of combating fungal infections. Oils of Carum copticum and Thymus vulgaris have long been used in ethnomedicine for ailments of various fungal infections. Since their activity has not been reported in particular against drug-resistant fungi, this study was aimed to evaluate the effects of oils of C. copticum and T. vulgaris on the growth and virulence of drug-resistant strains of Aspergillus spp. and Trichophyton rubrum. The gas chromatography-mass spectrometry analysis revealed thymol constituting 44.71% and 22.82% of T. vulgaris and C. copticum, respectively. Inhibition of mycelial growth by essential oils was recorded in the order of thymol > T. vulgaris > C. copticum against...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anti-elastase activity; Arthroconidia; Synergy; Thymol; Virulence.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200021
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Characterization and comparison of Serratia marcescens isolated from edible cactus and from silkworm for virulence potential and chitosan susceptibility BJM
Li,Bin; Yu,Rongrong; Liu,Baoping; Tang,Qiaomei; Zhang,Guoqing; Wang,Yanli; Xie,Guanlin; Sun,Guochang.
Representative strains of Serratia marcescens from an edible cactus plant and silkworms were characterized and a comparison based on their cellular fatty acid composition, 16S rRNA and groE gene sequence analysis as well as silkworm virulence and chitosan susceptibility was carried out. Results from this study indicate that there are no significant differences between the phenotypic and molecular characterization, virulence and chitosan susceptibility of the S. marcescens strains from the cactus plant and silkworms. Silkworms inoculated with S. marcescens from either plant or silkworm resulted in nearly 100% mortality. Chitosan solution exhibited strong antibacterial activity against S. marcescens. This activity increased with the increase of chitosan...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Serratia marcescens; Cactus; Silkworm; Characterization; Virulence; Chitosan susceptibility.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100013
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Characterization of Nomuraea rileyi strains using polymorphic DNA, virulence and enzyme activity BABT
Vargas,Lúcia Rosane Bertholdo; Rossato,Marcelo; Ribeiro,Rute Terezinha da Silva; Barros,Neiva Monteiro de.
The characterization of entomopathogenic microorganisms is important for the selection of more effective strains for use in integrated pest-control programs. Five Nomuraea rileyi strains (SA86101, GU87401, SR86151, CG128 and VA9101) were characterized using random amplified polymorphic DNA (RAPD) analysis, virulence studies and assessment of chitinolytic and proteolytic activity. RAPD analysis divided the strains into two groups with a similarity coefficient of 0,76%, group 1 consisting of strains SA86101, GU87401 and SR86151 and group 2 of strains CG128 and VA9101. The LT50 varied from 165h with strain VA9101 to 246h with strain GU87401. Chitinolytic and proteolytic activity of the fungi after 144h growth in minimal medium were tested using colloidal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nomuraea rileyi; Anticarsia gemmatalis; Chitinase; Protease; RAPD; Virulence.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100003
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Characterization of the virulence, growth temperature and antibiotic resistance of the Campylobacter jejuni IAL 2383 strain isolated from humans BJM
Fonseca,B.B.; Rossi,D.A.; Maia,C.A.; Nalevaiko,P.C.; Melo,R.T.; Cuccato,L.P.; Beletti,M.E..
The objective of this study was to characterize the C. jejuni IAL2383 strain isolated from humans in Brazil. Transcripts for the racR, dnaJ and ciaB genes were found and flaA, plda and cadF genes were present in the genome and bacteria was sensitive to most of the important antimicrobials used to treat humans. C. jejuni IAL2383 is a good experimental model to analyze the interactions with cells.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Virulence; Experimental model; Thermotolerance; Campylobacter jejuni.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100039
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Community-associated methicillin-resistant Staphylococcus aureus in non-outbreak skin infections BJM
Bonesso,Mariana Fávero; Marques,Silvio Alencar; Camargo,Carlos Henrique; Fortaleza,Carlos Magno Castelo Branco; Cunha,Maria de Lourdes Ribeiro de Souza da.
The aim of this study was to determine the prevalence of Staphylococcus aureus and risk factors for the acquisition of MRSA (Methicillin Resistant Staphylococcus aureus) as the main cause of skin and soft tissue infections. S. aureus were characterized for the presence of PVL, TSST-1 and mecA genes. SCCmec typing was carried out in mecA positive strains and PFGE was performed only in these strains. During the study period, 127 outpatients attending a dermatology clinical the Botucatu Medical School, a regional tertiary hospital in Botucatu, Sao Paulo, Brazil, were diagnosed with active skin infections. A total 66 (56.9%) S. aureus strains were isolated. The methicillin resistance gene mecA was detected in seven (10.6%) S. aureus strains. The SCCmec types...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Resistance; Skin infections; Virulence; Epidemiology.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400034
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Comparative analysis of Vibrio splendidus-related strains isolated during Crassostrea gigas mortality events ArchiMer
Le Roux, Frédérique; Gay, Melanie; Lambert, Christian; Waechter, Magali; Poubalanne, Saravanne; Chollet, Bruno; Nicolas, Jean-louis; Berthe, Franck.
French mollusc production is based mainly on the Pacific cupped oyster, Crassostrea gigas. Since 1991, annual mass mortality of juveniles has been reported during summer months. These recurring episodes concern professionals who fear that like Portugese oyster, C. angulata, C gigas could in turn disappear following one of these epizooties. Previously, bacteriological analysis of moribund oyster juveniles yielded an isolate of a Vibrio splendidus biovar H strain, named TNEMF6. This isolate was demonstrated to be pathogenic to Crassostrea gigas spat by experimental challenge. To study the association between summer oyster mortality and presence of TNEMF6 cluster strains, Vibrionaceae fauna were isolated from infected spat along the French Atlantic coast...
Tipo: Text Palavras-chave: Vibrio splendidus; Virulence; Epidemiology; PCR RFLP; Oyster mortality.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-565.pdf
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Comparative genomics of pathogenic lineages of Vibrio nigripulchritudo identifies virulence-associated traits ArchiMer
Goudenege, David; Labreuche, Yannick; Krin, Evelyne; Ansquer, Dominique; Mangenot, Sophie; Calteau, Alexandra; Medigue, Claudine; Mazel, Didier; Polz, Martin F.; Le Roux, Frederique.
Vibrio nigripulchritudo is an emerging pathogen of farmed shrimp in New Caledonia and other regions in the Indo-Pacific. The molecular determinants of V. nigripulchritudo pathogenicity are unknown; however, molecular epidemiological studies have suggested that pathogenicity is linked to particular lineages. Here, we performed high-throughput sequencing-based comparative genome analysis of 16 V. nigripulchritudo strains to explore the genomic diversity and evolutionary history of pathogen-containing lineages and to identify pathogen-specific genetic elements. Our phylogenetic analysis revealed three pathogen-containing V. nigripulchritudo clades, including two clades previously identified from New Caledonia and one novel clade comprising putatively...
Tipo: Text Palavras-chave: Horizontal gene transfer; Nigritoxin; Phylogeny; Shrimp; Virulence; Vibrio.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00160/27117/28369.pdf
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