|
|
|
|
| |
|
|
| SOUSA, A. L. M.; PINHEIRO, R. R.; ARAÚJO, J. F.; SANTOS, V. W. S.; AZEVEDO, D. A. A.; PEIXOTO, R. M.; SOUZA, V. de; ANDRIOLI, A.; DAMASCENO, E. M.; DANTAS, T. V. M.; TEIXEIRA, M. F. S.. |
| Abstract: The aim of this study was to evaluate in vitro and in vivo the effect of sodium dodecyl sulfate (SDS) on the caprine lentivirus (CLV) in colostrum and milk. This was performed to develop a practical and efficient method of blocking the lactogenic transmission of the virus. In the in vitro experiment, colostrum and milk were treated with 0.25%; 0.50% and 1% SDS. Then, somatic cells of colostrum and milk were submitted to co-culture with caprine synovial membrane cells (CSM). In the in vivo test, goats were fed with colostrum and milk provided from CLV-positive goats treated with SDS in the same concentrations used in the in vitro experiment. Animals were tested by nested polymerase chain reaction (nPCR) and Western blot (WB) assays. In the in... |
| Tipo: Artigo de periódico |
Palavras-chave: Lentivirose; Sistema monociticofagocitário; Inativação viral; Surfactante; Lentiviroses de pequenos ruminantes; Caprino; Goats; Lentivirus; Animal diseases; Colostrum; Virology; Viral antibodies; Virus transmission; Disease transmission; Caprine arthritis encephalitis virus. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1095441 |
| |
|
|
| AMARAL, G. P.; MOREIRA, A. C. G. C.; FERREIRA, A. B. M.; DAMASCENO, M. S.; ARAÚJO, J. F.; ANDRIOLI, A.. |
| A Artrite Encefalite Caprina (CAE) é uma enfermidade crônica, causada por vírus pertencente ao gênero Lentivirus e família Retroviridae, que ocasiona grandes perdas econômicas à caprinocultura. A transmissão pela via intrauterina do lentivírus de pequenos ruminantes (LVPR), foi conjecturada em 11% dos animais recém-nascidos de matrizes ovinas infectadas, sendo o sangue coletado antes dos recém-nascidos receberem o colostro. Em estudo com células de cordão umbilical de ovinos constatou-se permissividade à multiplicação do LVPR em cultivo. Desta forma, a transmissão vertical necessita de mais estudos, pois interfere nos programas de controle da CAE. O objetivo deste trabalho foi avaliar a presença de DNA pró-viral nas células de cordão umbilical de cabritos... |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: Cultivo celular; Transmissão vertical; Ovino; Sheep diseases; Lentivirus; Virus transmission; Brazil. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1116485 |
| |
|
|
| SOUZA, T. S. de; PINHEIRO, R. R.; LIMA, C. C. V. de; BRITO, R. L. L. de; AZEVEDO, D. A. A. de; DIAS, R. P.; SANTOS, V. W. S. dos; ANDRIOLI, A.; COSTA, J. N.. |
| Abstract: The objective of this study was to demonstrate iatrogenic transmission of small ruminant lentivirus (SRLV) from goats to sheep and horizontal transmission between sheep. The study was conducted on a farm with separate goat and sheep rearing, and animals were monitored for lentivirus occurrence by clinical examination and testing by immunoblotting (IB), agar gel immunodiffusion (AGID), and nested polymerase chain reaction (nPCR). Positive results had not been observed in the sheep flock until this study. Conversely, virus positive dairy goats were known. For this reason, the farm performed the caprine arthritis-encephalitis (CAE) control program. The study was designed with a sheep group that presented positive animals for SRLV by nPCR. It was... |
| Tipo: Artigo de periódico |
Palavras-chave: Lentivirose; Infecção cruzada; Artrite Encefalite Caprina; CAE; SRLV; Maedi-Visna; Ovino; Caprino; Infecção; Transmissão de Doença; Sheep diseases; Ovine lentivirus; Visna maedi virus; Caprine arthritis encephalitis virus; Cross infection; Virus transmission; Disease transmission. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1096947 |
| |
|
|
| CHEN, W.; HASEGAWA, D. K.; KAUR, N.; KLIOT, A.; PINHEIRO, P. V.; LUAN, J.; STENSMYR, M. C.; ZHENG, Y.; LIU, W.; SUN, H.; XU, Y.; LUO, Y.; KRUSE, A.; YANG, X.; KONTSEDALOV, S.; LEBEDEV, G.; FISHER, T. W.; NELSON, D. R.; HUNTER, W. B.; BROWN, J. K.; JANDER, G.; CILIA, M.; DOUGLAS, A. E.; GHANIM, M.; SIMMONS, A. M.; WINTERMANTEL, W. M.; LING, K.-S.; FEI, Z.. |
| Background: The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is among the 100 worst invasive species in the world. As one of the most important crop pests and virus vectors, B. tabaci causes substantial crop losses and poses a serious threat to global food security. Results: We report the 615-Mb high-quality genome sequence of B. tabaci Middle East-Asia Minor 1 (MEAM1), the first genome sequence in the Aleyrodidae family, which contains 15,664 protein-coding genes. The B. tabaci genome is highly divergent from other sequenced hemipteran genomes, sharing no detectable synteny. A number of known detoxification gene families, including cytochrome P450s and UDP-glucuronosyltransferases, are significantly expanded in B. tabaci. Other expanded gene families,... |
| Tipo: Artigo de periódico |
Palavras-chave: Polyphagy; Whitefly; Draft genome; Bemisia tabaci; Mosca branca; Praga de planta; Plant pests; Virus transmission; Insecticide resistance. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1058708 |
| |
|
|
| LIMA, C. C. V. de; AYRES, M. C. C.; PINHEIRO, R. R.; COSTA, N. C.; SOUZA, T. S. de; ANDRIOLI, A.; AZEVEDO, D. A. A.; SANTOS, V. W. S. dos. |
| Abstract: This study was conducted to evaluate caprine arthritis encephalitis virus (CAEV) transmission among sheep using 15 lambs that were distributed in 2 experimental groups. The exposed group consisted of 10 lambs that remained with their mothers, who were experimentally infected with CAEV. The non-exposed group was characterized as the control group and was comprised of 5 lambs that remained with their CAEV-negative mothers. Blood samples were collected monthly from birth until 1 year of life. To evaluate the transmission, an agar gel immunodiffusion test (AGID), enzyme immunoassay (ELISA), immunoblotting (IB), and nested polymerase chain reaction (nPCR) techniques were used. The non-exposed group was negative in all of the tests throughout the whole... |
| Tipo: Artigo de periódico |
Palavras-chave: Lentivirose; CAEV; LVPR; MVV; Transmissão intra-espécie; Intra-species transmission; Ovino; Doença Animal; Transmissão de Doença; Cordeiro; Sheep diseases; Lambs; Disease transmission; Caprine arthritis encephalitis virus; Virus transmission; Ovine lentivirus; Maedi-visna disease. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1096081 |
| |
|
|
| PINHEIRO-LIMA, B.; PEREIRA-CARVALHO, R. C.; ALVES-FREITAS, D. M. T.; KITAJIMA, E. W.; VIDAL, A. H.; LACORTE, C.; GODINHO, M. T.; FONTENELE, R. S.; FARIA, J. C.; ABREU, E. F. M.; VARSANI, A.; RIBEIRO, S. G.; MELO, F. L.. |
| Tipo: Artigo de periódico |
Palavras-chave: Common bean; Whitefly; Vector; Virus evolution; Phaseolus Vulgaris; Bemisia Tabaci; Cytorhabdovirus; Virus transmission. |
| Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125482 |
| |
|
|
|
