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Registros recuperados: 41
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Activity of the antiarrhythmic drug amiodarone against Leishmania (L.) infantum: an in vitro and in vivo approach J. Venom. Anim. Toxins incl. Trop. Dis.
Pinto,Erika G.; Tempone,Andre G..
Abstract Background: Considering the high toxicity and limited therapies available for treating visceral leishmaniasis (VL), the drug repositioning approach represents a faster way to deliver new therapies to the market. Methods: In this study, we described for the first time the activity of a potent antiarrhythmic, amiodarone (AMD), against L. (L.)infantum and its in vitro and in vivo activity. Results: The evaluation against promastigotes has shown that amiodarone presents leishmanicidal effect against the extracellular form, with an IC50 value of 10 μM. The activity was even greater against amastigotes in comparison with promastigotes with an IC50 value of 0.5 μM. The selectivity index in relation to the intracellular form demonstrated that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmania; Visceral leishmaniasis; Amiodarone; In vitro; In vivo.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100320
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American visceral leishmaniasis: disease control strategies in dracen microregion in alta paulista, SP, Brazil J. Venom. Anim. Toxins incl. Trop. Dis.
D'Andrea,LAZ; Camargo-Neves,VLF; Sampaio,SMP; Kronka,SN; Sartor,IF.
Despite measures adopted to control American visceral leishmaniasis (AVL), the disease is spreading in a fast and worrying way throughout western São Paulo state. The aim of this work was to study the variables involved in the disease cycle as well as the effectiveness of controlling measures. The study was carried out in the microregion of Dracena, which is composed of twelve cities and belongs to Alta Paulista, a region of western São Paulo. The necessary data were provided by the Superintendence for Endemic Disease Control and Adolfo Lutz Institute, Regional Laboratory of Presidente Prudente. From August 2005 to January 2008, the following factors were observed: detection of phlebotomine sandflies in the cities and periods in which dogs or humans were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Visceral leishmaniasis; Seroprevalence; Control strategies; São Paulo; Brazil.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200012
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Anti-leishmania antibodies in cerebrospinal fluid from dogs with visceral leishmaniasis BJMBR
Lima,V.M.F.; Gonçalves,M.E.; Ikeda,F.A.; Luvizotto,M.C.R.; Feitosa,M.M..
Visceral leishmaniasis in Brazil is caused by Leishmania (Leishmania) chagasi and the dog is its most important reservoir. The clinical features in dogs include loss of weight, lymphadenopathy, renal failure, skin lesions, fever, hypergammaglobulinemia, hepatosplenomegaly, anemia, and, rarely, neurological symptoms. Most infected animals develop active disease, characterized by high anti-leishmania antibody titers and depressed lymphoproliferative ability. Antibody production is not primarily important for protection but might be involved in the pathogenesis of tissue lesions. An ELISA test was used to determine if there is an association between neurological symptoms and the presence of anti-L. chagasi antibodies in cerebrospinal fluid (CSF). Thirty serum...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Leishmania (Leishmania) chagasi; Visceral leishmaniasis; Cerebrospinal fluid; Dogs; Antibodies.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400010
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Application of direct agglutination test and fast agglutination screening test for serodiagnosis of visceral leishmaniasis in endemic area of Minas Gerais, Brazil OAK
Silva, E. S.; Schalig, H. D. F. H.; Kroon, N. C. M.; Schoone, G. J.; Gontijo, C. M. F.; Pacheco, Raquel S.; Brazil, R. P..
We have tested an indirect fluorescence technique (IFAT), fast agglutination screening test (FAST) and direct agglutination test (DAT) for the detection of anti-Leishmania antibodies in serum and blood samples from patients with visceral and cutaneous leishmaniasis in Brazil. For visceral leishmaniasis the results obtained with DAT showed a sensitivity and specificity of 100% with a cut-off value of 1:800. The blood and serum samples could also be clearly read in FAST using a 1:100 dilution with the same high sensitivity. Both DAT and FAST were not able to detect significant amounts of antibodies in samples from cutaneous leishmaniasis patients.
Palavras-chave: Visceral leishmaniasis; Diagnosis; DAT; FAST.
Ano: 2001 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/140
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Are opossums a relevant factor associated with asymptomatic Leishmania infection in the outskirts of the largest Brazilian cities? BJID
Carranza-Tamayo,César Omar; Werneck,Guilherme Loureiro; Romero,Gustavo Adolfo Sierra.
Abstract A population survey was conducted to explore the prevalence and factors associated with Leishmania infection in the Fercal region of the Federal District. The Fercal region is a group of neighborhoods in Brasília in which the first cases of visceral leishmaniasis were described. Leishmania infection was established by a positive leishmanin test. Although other tests were performed in the study (an immunochromatographic assay (Kalazar detect®) and a molecular assay), only the leishmanin skin test provided sufficient results for the measurement of the disease prevalence. Data on the epidemiological, clinical and environmental characteristics of individuals were collected along with the diagnostic tests. After sampling and enrollment, seven hundred...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Visceral leishmaniasis; Brazil; Didelphis albiventris; Leishmanin.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000200119
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Canine Visceral Leishmaniasis in a Doberman OAK
Sasani, F.; Jamshidi, Sh.; Mohebbali, M..
Palavras-chave: Dog; Doberman; Visceral leishmaniasis.
Ano: 1999 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/323
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CD4+ T cells participate in the nephropathy of canine visceral leishmaniasis BJMBR
Costa,F.A.L.; Guerra,J.L.; Silva,S.M.M.S.; Klein,R.P.; Mendonça,I.L.; Goto,H..
Renal involvement in visceral leishmaniasis (VL) is very frequent. The renal lesions of humans and dogs are similar but their pathogenesis has not been clearly elucidated. There is growing evidence that the cellular immune response is involved in the pathogenesis of immunologically mediated glomerulonephritis. Since T cells could participate in the pathogenesis of nephropathy, in the present study we investigated the possible involvement of CD4+ and CD8+ T cells in the nephropathy of canine VL. Six dogs naturally infected with Leishmania (Leishmania) chagasi from the endemic area in the Northeast of Brazil, the town of Teresina in the State of Piauí, were studied. An expressive inflammatory infiltrate of CD4+ T cells both in glomeruli and in interstitium...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Visceral leishmaniasis; Dog; Leishmania (Leishmania) chagasi; Nephropathy; Immunoglobulin; T cells.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200009
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Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice J. Venom. Anim. Toxins incl. Trop. Dis.
Machado,JG; Hoffmann,JL; Krause,VLK; da Silva,AV; Dias-Melicio,LA; Langoni,H.
Leishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-γ, IL-2, IL-4 and IL-10) by sandwich ELISA. Parasite loads in the spleen and liver were determined by means of culture microtitration. Immunosuppressed groups showed statistically lower spleen weight and CD4-cell percentage in blood on the day of infection and produced Th1 and Th2 cytokines on other days of the study. The other infected groups, weather immunosupressed or not, also produced Th1 and Th2 cytokines. Parasite loads in the spleen and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Visceral leishmaniasis; Immunosuppression; Immunopathology; Mice; Interleukins.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100013
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Clinical and histopathological features of myocarditis in dogs with visceral leishmaniasis Arq. Bras. Med. Vet. Zootec.
Santos,F.P. dos; Pascon,J.P.E.; Pereira,D.T.P.; Anjos,B.L.; Mistieri,M.L.A.; Silveira,I.D.; Porciuncula,M.L..
Visceral leishmaniasis (VL) is a cosmopolitan parasitic zoonosis that can promote myocarditis and heart rate changes in canine and human hosts. Thus, histopathological aspects of the myocardium and clinical, hematological, biochemical, radiological and electrocardiographic data were evaluated in a group of 36 dogs naturally infected with VL (VLG) and compared to data from 15 non-infected dogs (CG=Control Group). A prevalence of asymptomatic dogs was present in the CG (100%) and polysymptomatic dogs in the VLG (66%). In addition, two dogs in the VLG demonstrated systolic murmurs in the mitral valve region: one with a II/VI intensity and the other with a III/VI intensity. The mean values of RBC, hemoglobin and hematocrit were lower in dogs in VLG and were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Electrocardiography; Leishmania sp.; Visceral leishmaniasis; Dogs.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601519
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Clinical presentation and renal evaluation of human visceral leishmaniasis (kala-azar): a retrospective study of 57 patients in Brazil BJID
Daher,Elizabeth F.; Evangelista,Leandro F.; Silva Júnior,Geraldo B.; Lima,Rafael S.A.; Aragão,Eveline B.; Arruda,Germana A.J.C.; Galeano,Noemi M.F.; Mota,Rosa M.S.; Oliveira,Rodrigo A.; Silva,Sônia L..
Visceral leishmaniasis is an endemic disease caused by various species of Leishmania. We made a retrospective study of 57 consecutive patients with visceral leishmaniasis in Brazil. Patients with visceral leishmaniasis were identified using the registries of the São José Infectious Diseases Hospital. The sample was divided into two groups: patients with serum creatinine (Scr) <1.3mg/dL and Scr > 1.3mg/dL. We compared these two groups for differences in clinical manifestations and laboratory features. Patients' mean age was 28 ± 18 years old; 74% were male. The main clinical symptoms and signs presented in the initial evaluation were: fever (97%), splenomegaly (96.4%), weight loss (95.5%), pallor (93.6%), cough (89.7%), hepatomegaly (87.2%), asthenia...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Visceral leishmaniasis; Kala-azar; Symptoms; Acute renal failure.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400015
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Comparison of conventional methods for diagnosis of visceral leishmaniasis in children of the Center-West Region of Brazil BJID
Brustoloni,Yvone M.; Cunha,Rivaldo V.; Dorval,Maria Elizabeth; Oshiro,Elisa T.; Pontes,Elenir R.J.C.; Oliveira,Ana Lúcia L.; Hillebrand,Lilian; Ribeiro,Luis Felipe.
In Brazil, sophisticated techniques currently employed for diagnosis of visceral leishmaniasis, such as polymerase chain reaction-based assays, are only available in major research centers, whereas conventional methods are still used in many areas where the disease occurs. In the state of Mato Grosso do Sul, in the country's Center-West Region, visceral leishmaniasis has recently emerged in many cities, and duration of the disease, from the onset of symptoms to diagnosis, has been short. Considering that results of diagnostic tests may depend on the phase of the disease, we compared direct examination of bone marrow aspirates (BMAs), BMA culture, and serology by Indirect Immunofluorescence Antibody Test (IFAT) for diagnosis in children, according to time...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Visceral leishmaniasis; Kala-azar; Diagnosis; Direct microscopy; Immunological tests.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100023
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Comparison of different diagnostic tests in dogs uninfected and naturally infected with visceral leishmaniasis J. Venom. Anim. Toxins incl. Trop. Dis.
Sassaki,CY; Colodel,MM; Ferreira,I; Nogueira,FS; Lucheis,SB; Langoni,H; Rocha,NS.
Uninfected dogs (n = 10) and those naturally infected with leishmaniasis (n = 10) were subjected to several diagnostic tests, namely: hemoculture, polymerase chain reaction (PCR) of hemoculture, indirect immunofluorescence (RIFI), cytological examination of lymph node aspirate, culture of lymph node aspirate and PCR of lymph node aspirate. RIFI - followed by PCR of lymph node aspirate culture - presented more positive results in infected dogs than in uninfected ones. In infected animals, RIFI was more effective than PCR of lymph node aspirate culture. There was no statistical difference in positivity between RIFI and hemoculture; lymph node aspirate culture/cytological examination of lymph node aspirate and PCR of hemoculture; and between PCR of lymph node...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diagnosis; Dogs; Visceral leishmaniasis.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300015
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Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review J. Venom. Anim. Toxins incl. Trop. Dis.
Paiva-Cavalcanti,M; Regis-da-Silva,CG; Gomes,YM.
In recent years, the polymerase chain reaction (PCR) technique has significantly advanced towards expanding its use and versatility by working with quantitative real-time PCR (qPCR). Data from the literature show that both methods present interesting characteristics for the diagnosis of visceral leishmaniasis. The benefits of qPCR in relation to conventional PCR include speed, reproducibility and quantitative ability. In addition to operational advantages, qPCR is more sensitive and reproducible and may replace conventional PCR in diagnostic routines. Regarding visceral leishmaniasis, the possibility of deployment of real-time PCR in highly complex diagnoses (reference services) in endemic areas will facilitate a swift and safe return for patients....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diagnosis; Molecular diagnosis; Visceral leishmaniasis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400004
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Congenitally transmitted visceral leishmaniasis: report of two brazilian human cases BJID
Mescouto-Borges,Myrlena Regina Machado; Maués,Érika; Costa,Dorcas Lamounier; Pranchevicius,Maria Cristina da Silva; Romero,Gustavo Adolfo Sierra.
Visceral leishmaniasis is a relevant public health problem worldwide. Most of the reported cases in Latin America are from Brazil. Herein we report two human cases of congenitally transmitted visceral leishmaniasis in two patients who developed symptoms during pregnancy. The diagnosis was made by visual examination of Leishmania parasites in bone marrow aspirates of the mothers and by detecting parasite kDNA in bone marrow samples of the newborn children using polymerase chain reaction.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Congenital transmission; Visceral leishmaniasis; Leishmania infantum; Pregnancy.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200024
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Detection of immunoglobulin G in the lung and liver of hamsters with visceral leishmaniasis BJMBR
Mathias,R.; Costa,F.A.L.; Goto,H..
Several organs are affected in visceral leishmaniasis, not only those rich in mononuclear phagocytes. Hypergammaglobulinemia occurs during visceral leishmaniasis; anti-Leishmania antibodies are not primarily important for protection but might be involved in the pathogenesis of tissue lesions. The glomerulonephritis occurring in visceral leishmaniasis has been attributed to immune complex deposition but in other organs the mechanism has not been studied. In the current study we demonstrated the presence of IgG in the lung and liver of hamsters with visceral leishmaniasis. Hamsters were injected intraperitoneally with 2 x 10(7) amastigotes of Leishmania (Leishmania) chagasi and the presence of IgG in the liver and lung was evaluated at 7, 15, 30, 45, 80 and...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Leishmania (Leishmania) chagasi; Visceral leishmaniasis; Immunopathology; Immunoglobulin G; Liver; Lung.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400015
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Detection of specific IgE antibodies in parasite diseases BJMBR
Souza-Atta,M.L.B.; Araújo,M.I.; D'Oliveira Júnior,A.; Ribeiro-de-Jesus,A.; Almeida,R.P.; Atta,A.M.; Carvalho,E.M..
Activation of Th1 or Th2 cells is associated with production of specific immunoglobulin isotypes, offering the opportunity to use antibody measurement for evaluation of T cell function. Schistosomiasis and visceral leishmaniasis are diseases associated with Th2 activation. However, an IgE response is not always detected in these patients. In the present study we evaluated specific IgE antibodies to S. mansoni and L. chagasi antigens by ELISA after depletion of serum IgG with protein G immobilized on Sepharose beads or RF-absorbent (purified sheep IgG antibodies anti-human IgG). In schistosomiasis patients, specific IgE to SWAP antigen was demonstrable in only 10 of 21 patients (48%) (mean absorbance ± SD = 0.102 ± 0.195) when unabsorbed serum was used....
Tipo: Info:eu-repo/semantics/other Palavras-chave: IgE antibodies; Immunoassays; Schistosomiasis mansoni; Visceral leishmaniasis; Kala-azar.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900008
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Electroantennographic responses of the Lutzomyia (Lutzomyia) longipalpis (Lutz &amp; Neiva) (Diptera: Psychodidae) to 1-octen-3-ol Neotropical Entomology
SANT'ANA,ADSON L.; EIRAS,ALVARO E.; CAVALCANTE,REGINALDO R..
Octenol (1-octen-3-ol) is a kairomone used by haematophagous insects to locate their vertebrate hosts. However, effect of 1-octen-3-ol on Lutzomyia (Lutzomyia) longipalpis (Lutz &amp; Neiva) has never been studied. The present work evaluated the electrophysiological (EAG) responses of female L. (Lutzomyia) longipalpis. Air current, air pulse and solvent (hexane) pulse were used as control stimuli. The logarithmic concentrations of 1-octen-3-ol 10 a 10(6) etag/50µl of solvent were tested. Significant olfactory responses were observed in the concentration of 1-octen-3-ol from 10³ etag/50ul with the greatest response at concentration of 10(6) etag/50ul (-3,33mV). Dose-dependency was observed, as the concentration increased, so did the electrophysiological...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Chemoreception; Olfaction; Semiochemicals; Visceral leishmaniasis.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2002000100002
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Epidemiological aspects of the first human autochthonous visceral leishmaniosis cases in Porto Alegre, Brazil BJID
Mahmud,Ibrahim Clós; Piassini,Letícia de Araujo Saraiva; Motta,Fabrizio; Behar,Paulo Renato Petersen; Souza,Getúlio Dornelles.
ABSTRACT Human visceral leishmaniasis is a growing anthropozoonosis in Brazil, and particularly in the southern region of the country. It is an infectious disease transmitted to humans, dogs and other animals in urban and rural areas of the Americas, mainly due to the bite of Lutzomya longipalpis infected with Leishmania infantum. This article aims to portray the current epidemiological situation of the human visceral leishmaniasis arrival in Porto Alegre city, located in the southern region of Brazil. It is a descriptive study, a case series and a critical review. Six human cases with human visceral leishmaniasis were notified by the date of conclusion of the study, all human visceral leishmaniasis cases were diagnosed at late stage, leading to four deaths.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Visceral leishmaniasis; Fever caused by sandflies; Communicable diseases; Leishmania infantum.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000200124
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Epidemiological profile of leishmaniasis at a reference service in the state of Alagoas, Brazil, from january 2000 to september 2008 BJID
Nunes,Willian da Silva; Araújo,Sócrates Rios; Calheiros,Cláudia Maria Lins.
Leishmaniasis is a parasitic disease found in the continents of Europe, Asia, Africa and the Americas. In Brazil, the disease is an important public health problem, occurring in most states, but mainly in the Northeast region of the country, with the state of Alagoas having a significant number of confirmed cases. The present study establishes and discusses the epidemiological profile of cases of leishmaniases treated at the Reference Center of Alagoas, Hospital Escola Hélvio de Farias Auto, between January 2000 and October 2008. During thisperiod, records of 2,104 patients notified to the hospital Epidemiological Surveillance Center were reviewed. The epidemiology of leishmaniasis, both visceral and tegumentary, has shown a profile that has changed over...
Tipo: Info:eu-repo/semantics/article Palavras-chave: American tegumentary leishmaniasis; Visceral leishmaniasis; Epidemiology.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000400005
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Fatores de risco associados à ocorrência da leishmaniose visceral na área urbana do município de Campo Grande/MS. Repositório Alice
TELES, A. P. S.; HERRERA, H. M.; AYRES, F. M.; BRAZUNA, J. C. M.; ABREU, U. G. P. de.
A Leishmaniose Visceral (LV) é considerada uma enfermidade dinâmica, dadas as alterações em seu padrão de transmissão e plasticidade do vetor. O objetivo deste estudo foi associar fatores de risco à ocorrência da LV no perímetro urbano do município de Campo Grande/MS utilizando-se de análises estatísticas e espaciais. Observou-se uma correlação positiva entre os casos de Leishmaniose Visceral Canina (LVC) e Leishmaniose Visceral Humana (LVH), e entre a LVC e a área do bairro. Uma correlação negativa foi observada entre a densidade da rede de esgotamento sanitário e a LVC. A álgebra de mapas espacializou as áreas de risco para a ocorrência da LV. As análises mostram que existem condições favoráveis para a transmissão dessa zoonose e os riscos estão...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Saúde ambiental; Análise espacial; Leishmaniose; Doença animal; Visceral leishmaniasis; Public health.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1032924
Registros recuperados: 41
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