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Cryopreservation of Canine Ovaries by Vitrification OAK
ISHIJIMA, Takako; KOBAYASHI, Yoshiyasu; LEE, Dong-Soo; UETA, Yoshiko Yanagimoto; MATSUI, Motozumi; LEE, Jung-Youn; SUWA, Yoshinori; MIYAHARA, Kazuro; SUZUKI, Hiroshi; 松井, 基純; 宮原, 和郎; 鈴木, 宏志; 古林, 与志安.
The cryopreservation of ovarian tissues is a technology with significant potential for the preservation of the genetic resource materials of working dogs, including guide dogs for the blind. However, no attempt has been reported on cryopreservation of the canine ovary. Thus, we evaluated a vitrification method for cryopreservation of canine ovaries and determined the potential functionality of vitrified-warmed canine ovaries by means of transplantation into non-obese diabetic-severe combined immunodeficiency (NOD-SCID) mice. All ovarian tissues cryopreserved by vitrification were morphologically normal in terms of histology. Cryopreserved ovaries were transplanted into the ovarian bursa of the NOD-SCID mice, and the xenografts were recovered from 23 of 23...
Palavras-chave: Canine; Ovary transfer; Transplantation; Vitrification; Xenograft.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3023
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Cryopreservation of the mangaba tree (Hancornia speciosa Gomes): a protocol for long-term storage Agronomy
Santos, Paulo Augusto Almeida; Paiva, Renato; Silva, Luciano Coutinho; Souza, Ana Cristina de; Santana, Marlucia Cruz de; Silva, Diogo Pedrosa Corrêa da.
The aim of this study was to evaluate the efficiency of vitrification and droplet vitrification for the cryopreservation of Hancornia speciosa shoot tips. The shoot tips were subjected to four different periods of exposure (15, 30, 45 and 60 min.) to plant vitrification solution 2 (PVS2) before plunging into liquid nitrogen. We evaluated the regrowth of H. speciosa shoot tips that were cryopreserved by the classical vitrification technique and by the droplet vitrification technique. Shoot tips were submitted to different periods of pre-culture (absence, 24 or 48h) in a medium containing 0.3 M sucrose prior to cryopreservation. With a PVS2 exposure period of 60 min., significant differences were observed between the two techniques used in this study with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.00.00.00-4 - ciências agrárias; 2.03.03.00-9 - fisiologia vegetal; Native species; In vitro conservation; Recalcitrant seeds; Plant genetic diversity conservation; Vitrification; Droplet vitrification Conservação in vitro; Criopreservação vegetal.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19573
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Cryotolerance and pregnancy rates after exposure of bovine in vitro-produced embryos to forskolin and linoleic acid before vitrification. Repositório Alice
MENEGHEL, M.; DALL ACQUA, P. C.; AMBROGI, M.; GOUVÊA, L. M.; ACHILLES, M. A.; LEÃO, B. C. S.; NOGUEIRA, E.; ROCHA-FRIGONI, N. A. S.; MINGOTI, G. Z..
The objective of the present study was to evaluate the effects of supplementation of in vitro culture (IVC) medium with drugs that stimulates the lipolysis (Forskolin: Forsk) and inhibit the lipogenesis (Linoleic Acid LA) on the intracytoplasmic lipid content and cryotolerance of bovine embryos (Experiment 1), as well as to evaluate the effect of treatment of embryos with Forsk on the pregnancy rates after transfer to synchronized recipients (Experiment 2).
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Linoleic acid; Reprodução animal; Criopreservação; Cryopreservation; Forskolin; Vitrification; Animal reproduction.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1055135
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Cryotop and development of vitrified immature bovine oocytes Arq. Bras. Med. Vet. Zootec.
Hajarian,H; Wahid,H; Rosnina,Y; Daliri,M; Dashtizad,M; Karamishabankareh,H; Abas Mazni,O.
The effectiveness of different cryodevices (open-pulled straw (OPS), electron microscopy grid (EMG), and Cryotop was evaluated for vitrification of immature bovine oocytes. Polar body, metaphase II stage (MII), survivability, and subsequent developmental rates were determined. Only oocytes with four or five layers of cumulus cells were used. Oocytes were equilibrated in two vitrification solutions - 1: 10% DMSO + 10% ethylene glycol (EG) for 30-45sec and 2: 20% DMSO + 20% EG +0.5M sucrose for 25sec -, mounted on one of the cryodevices and directly plunged into liquid nitrogen for 10 days. Immature vitrified oocytes using Cryotop showed the highest rates of polar body extrusion (PB) and nuclear maturity (MII); 41 and 58% respectively. Vitrified oocytes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Immature oocyte; Vitrification; Cryodevice.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000100011
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Designing ex-situ conservation strategies for seeds storage of Piper aduncum and P. hispidinervum through cryopreservation and low-temperature techniques. Repositório Alice
SILVA, T. L. da; GOMES, H. T.; SCHERWINSKI-PEREIRA, J. E..
bitstream/item/170598/1/Designing-ex-situ-conservation-strategies-for-seeds-storage-of-Piper-aduncum-and-P-hispidinervum-through-cryopreservation-and-low-temperature.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Desiccation; Piperaceae; Germplasm conservation; Cryopreservation; Vitrification.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1084611
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Effect of ß-Mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos. Repositório Alice
PRADIEÉ, J.; CARDOSO, T. F.; SILVA, E. F.; GONÇALVES, A. O.; GASTAL, G. D. A.; ROSA, C. E.; MONDADORI, R. G.; PEGORARO, L. M. C.; VIEIRA, A. D.; LUCIA JUNIOR, T..
2016
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Sheep embryos; Antioxidant; Reactive oxygen species; Frozen ram sperm; Vitrification.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1053502
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Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations Biol. Res.
MOZDARANI,HOSSEIN; MORADI,SHABNAM Z.
This study was desµgned to investµgate the effect of vitrification and post-thaw survival and chromosomal aberrations caused by vitrification of vitrified 8-cell mouse embryos in comparison with a controligroup. To this purpose the survival rate and the frequency of chromosomal aberrations were assessed in frozen-thawed 8-cell mouse embryos after various storage durations in the presence of ethyleneiglycol as cryoprotectant. eight-cell mouse embryos were obtained from NMRI mice 3 days after mating. Retrieved embryos were transferred to vitrification solution containing ethyleneiglycol as cryoprotectant, then transferred into a vitrification straw using standard technique, and vitrified in liquid nitrogen. Sixigroups of embryos according to storage duration...
Tipo: Journal article Palavras-chave: Cryopreservation; Vitrification; Mouse embryo; Viability; Chromosome abnormalities.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400004
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Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos Arq. Bras. Med. Vet. Zootec.
Pradieé,J.; Cardoso,T.F.; Silva,E.F.; Gonçalves,A.O.; Gastal,G.D.A.; Rosa,C.E.; Mondadori,R.G.; Pegoraro,L.M.C.; Vieira,A.D.; Lucia Jr.,T..
ABSTRACT The effects of β-mercaptoethanol (BME) and cysteine on the viability and oxidative activity of ram sperm after thawing and on development in vitro and viability of vitrified sheep embryos were evaluated. Ejaculates from four rams were pooled and extended, composing six treatments: no antioxidants; 2mM BME; 5mM BME; 2mM BME and 5mM cysteine; 5mM BME and 5mM cysteine; and 5mM cysteine. Sperm motility, membrane and acrosome integrity, mitochondrial functionality, production of reactive oxygen species and total antioxidant capacity were similar across treatments (P>0.05). A medium with no antioxidant presented cleavage and blastocyst development rates (60.3% and 33.6%, respectively) similar (P>0.05) to those of a medium with 50μM BME and 600μM...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sheep embryos; Antioxidant; Reactive oxygen species; Frozen ram sperm; Vitrification.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501309
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Effects of EGTA and cytochalasin-B during freezing and vitrification of immature and mature bovine and rhesus monkey (M. mulatta) oocytes. Repositório Alice
YOUNIS, A. I.; KESKINTEPE, L.; SIMPLICIO, A. A.; GOULD, K.; BRACKETT, B. G..
Cryopreservation procedures have been shown to be extremely disruptive to the plasma membrane and intracellular organization of mammalian oocyte. Cytochalasin-B (Cyt-B) or EGT A may stabilize oocyte cytoskeletal elements prior to Cryopreservation and make the plasma membrane less rigid and more elastic to avoid injury during the osmotic stresses of freezing. Experiments were conducted to assess the morphological viability and developmental potential of bovine and rhesus monkey oocytes roter exposure to Cyt-B or EGTA prior to freezing and vitrification. ln the bovine experiments: The methods of oocyte isolation, in vitro maturation (IVM), in vitro fertilization (IVF) , and embryonic development (IVC) were as described elsewhere (Biol Reprod. 55:333-339,...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Oocisto; Citoxilasina B; Vitrificação; Oocyte freezing; Cytochalasin B; Bovino; Congelamento; Cultura in vitro; Bovine; Oocyte preservation; In vitro culture; Vitrification.
Ano: 1997 URL: http://www.alice.cnptia.embrapa.br/handle/doc/514939
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Survival rates of mouse blastocyst vitrified in dimethylformamide based solutions associated with ethylene glicol or 1-2 propanediol Ciência Rural
Villamil,Paula Rodriguez; Ongaratto,Felipe Ledur; Silva,Daniela Scherer da; Rodrigues,Berenice de Avila; Rodrigues,Jose Luiz.
The aim of this study was to determine the effect of dimethylformamide (DF) associated with ethylene glycol (EG) or 1-2 propanediol (PROH) during vitrification, on the in vitro development of mouse blastocysts. Cryoprotectant toxicity was evaluated exposing embryos into three different equilibrium solutions (ES) composed by DF, EG or PROH mixtures (10% v/v of each) in mPBS + 0.5% PVA at different interval times (1, 3 and 10min). In a second experiment, embryos were exposed to the same ES (either 1 or 3min), following for the three respectively vitrification solutions (VS) (20% v/v of each) for 30s. After 72 hours of in vitro culture, embryo hatching and expansion rates were similar for the ES1 and ES2 equilibration solutions during the time interval of 1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mouse; Blastocyst; Vitrification; Dimethylformamide; Glass micro pipettes.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011001100022
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Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content R. Bras. Zootec.
Carvalho,Bruno Pena; Costa,Fernanda de Queirós; Detoni,Danielly; Rosa,Felipe Borges; Dias,Angelo José Burla.
ABSTRACT This study evaluated the effect of the addition of conjugated linoleic acid (CLA) to in vitro culture on viability, lipid content, and cryoresistance of bovine embryos at different in vitro culture times. Cumulus oocyte complexes (N = 974) were maturated in vitro for 22 h. In vitro fecundation ensued for 18 h. Viable zygotes were cultivated in vitro in medium supplemented with CLA (100 mM) in the first 72 h (CLA-F), last 72 h (CLA-L), or throughout the culture period (CLA-T). Control embryos (control) were cultivated with no CLA. Embryos were cryopreserved by vitrification for subsequent analysis after devitrification. Effect of CLA on cryoresistance was assessed by cultivating embryos in synthetic oviductal fluid containing 5% fetal bovine serum....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryotolerance; Polyunsaturated fatty acid; Vitrification.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100602
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Use of ethyleneglycol monomethyl ether as cryoprotectant in vitrification of IVP bovine embryos. Repositório Alice
MADEIRA, E. M.; MION, B.; SILVA, J. F.; PEGORARO, L. M. C.; VIEIRA, A. D.; JUNIOR LUCIA, T..
2015
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Cryoprotectants; Ethylene glycol monomethyl ether; Vitrification.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1023094
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Vacuum-cooled liquid nitrogen increases the developmental ability of vitrified-warmed bovine oocytes Ciência Rural
Santos,Rodrigo Marques dos; Barreta,Marcos Henrique; Frajblat,Marcel; Cucco,Diego Córdova; Mezzalira,Joana Claudia; Bunn,Silvério; Cruz,Fabiano Buss; Vieira,Arnaldo Diniz; Mezzalira,Alceu.
The objective of this study was to determine the effects of vacuum-cooled liquid nitrogen on the development of vitrified immature (germinal vesicle stage; GV) and mature (metaphase II; MII) bovine oocytes after re-warming. Liquid nitrogen was exposed to either atmospheric pressure or to a vacuum (300mm Hg for 45sec); the latter decreased the temperature of the liquid nitrogen to -200°C. Partially denuded oocytes were vitrified either just after selection (GV) or after 22 hours of in vitro maturation (MII) in TCM 199 medium + 10% of estrous mare serum. For vitrification, oocytes were firstly exposed to an intermediate solution (10% EG + 10% DMSO) for 30sec, followed by the vitrification solution (20% EG + 20% DMSO + 0.5M sucrose) for 20sec. Groups of three...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vitrification; Cryopreservation; Oocytes; Bovine; Vacuum.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000500024
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培養基組成份對康乃馨試管苗玻璃質化的影響 Taiwan Agricultural Research Institute
古新梅; 蔡新聲; Hsin-Mei Ku; Hsin-Sheng Tsay.
[[abstract]]利用康乃馨組織培養所繁殖的枝條,探討培養基組成份造成試管苗玻璃質化的影響,結果顯示在不同的洋菜種類及濃度條件下,以液體培養的康乃馨玻璃質化最嚴重,其次為水晶洋菜(gelrite),其餘則依洋菜(Difco agar)濃度的增加,而玻璃質化的程度漸減。在cytokinins的比較試驗中發現,玻璃質化的比例隨BA(6-benzylamino purine)的濃度增高而增加,kinetin(6-furfurylamino purine)亦有相同的趨勢,但結果較BA不明顯。氨態氮與硝酸態氮的比例試驗中發現,隨著氨態氮比例的增加而玻璃質化有增高的趨勢。綜合以上結果顯示含有0.9% Difco-agar、0.5 ppm BA、NO3-:NH4+=1:1~2:1及3% sucrose之1/2 Murashige & Skoog基本鹽類之培養基,可大量繁殖康乃馨並避免嚴重的玻璃質化。玻璃質化枝條之外觀具水浸狀肉質莖葉,而組織切片則顯示莖部特化組織較正常者不分化,皮層及髓部薄壁細胞排列較不整齊,葉肉較正常者缺乏柵狀組織及海綿組織之分化,其間有大且空的細胞。
Palavras-chave: 康乃馨 組織培養 玻璃質化 組織切片 Carnation (Dianthus caryophyllus L.); Tissue culture; Vitrification; Cytohistological examination [[classification]]6.
Ano: 1994
Registros recuperados: 14
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