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| Registros recuperados: 13 | |
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| SOUSA, M. M. de; ANDRIOLI, A.; PINHEIRO, R. R.; ALVES, F. S. F.; SANTOS, V. W. S. dos; DAMASCENO, E. M.; ARAÚJO, J. F.; SOUSA, A. L. M. de; VIEIRA, L. da S.. |
| Abstract: Caprine arthritis encephalitis virus (CAEV) is a retrovirus that infects goats. This study evaluated the prevalence of CAEV in breeder goats from the states of Maranhão, Ceará, Piauí, Alagoas, Sergipe, Rio Grande do Norte, and Paraíba. We collected a total of 531 serum samples from 251 properties. On average, two male breeder goats were examined from each farm. Results from western blotting demonstrated that the prevalence of CAEV was 6.2% (32/513). In each state, the following prevalence values were found: Piauí, 5.9% (7/119); Maranhão, 2.0% (01/48); Sergipe, 7.1% (03/42); Alagoas, 17.6% (03/17); Rio Grande do Norte, 4.7% (05/105); Paraíba, 2.1% (02/94); and Ceará, 12.5% (11/34). We also conducted a univariate analysis to determine the risk... |
| Tipo: Artigo de periódico |
Palavras-chave: Artrite Encefalite Caprina; CAE; Caprino; Doença Animal; Lentivirus; Western blotting; Immunoblotting; Risk factors; Goat diseases; Goats; Disease prevalence. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1112530 |
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| Szargiki,Regiane; Castro,Edilene Alcântara de; Luz,Ennio; Kowalthuk,Wolodymir; Machado,Ângela Maria; Thomaz-Soccol,Vanete. |
| We evaluated the effectiveness of serological and parasitological methods for cutaneous leishmaniasis (CL) diagnosis in patients from the central region of Paraná state, southern Brazil. Five groups were compared: clinical diagnosis, parasitological diagnosis, communicants, inhabitants of a non-endemic area and carriers of other etiologies. Two antigens were prepared from promastigotes of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis for indirect immunofluorescence assay, ELISA and immunoblotting. The parasitological approaches detected 79.3% of the patients with a clinical diagnosis; the parasites were identified by PCR as L. (V.) braziliensis. Serological methods showed 95% sensitivity for homologous antigens. Immunoblotting... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cutaneous leishmaniasis; Immunodiagnosis; Antigen; Western blotting. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000100011 |
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| Gonzalez,E.T.; Oliva,G.A.; Norimine,J.; Cid de la Paz,V.; Echeverría,M.G.. |
| A western blotting (WB) procedure has been developed for detecting antibodies to bovine leukosis virus (BLV) in cattle sera. Two hundred and thirty three serum samples from naturally infected cattle with BLV virus and serial bleedings from experimentally BLV infected cows were used. An agar gel immunodiffusion test (AGID) was used for comparing with the results obtained by WB. The AGID positive sera showed a different degree of reactivity by WB test against the two most important viral antigens (gp51 and p24), or against one of them. Other proteins (gp30, p15, p12 and p10) were not detected with any AGID positive sera, being observed occasionally three bands corresponding to the p24 protein. Using sera obtained by BLV experimental inoculation, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine leukosis; Western blotting; Diagnosis. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400002 |
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| Xue,Deming; Xue,Yang; Niu,Zhipeng; Guo,Xueqiang; Xu,Cunshuan. |
| Abstract 14-3-3 proteins play a vital part in the regulation of cell cycle and apoptosis as signaling integration points. During liver regeneration, the quiescent hepatocytes go through hypertrophy and proliferation to restore liver weight. Therefore, we speculated that 14-3-3 proteins regulate the progression of liver regeneration. In this study, we analyzed the expression patterns of 14-3-3 proteins during liver regeneration of rat to provide an insight into the regenerative mechanism using western blotting. Only four isoforms (γ, ε, σ and τ/θ) of the 14-3-3 proteins were expressed in regenerative liver after partial hepatectomy (PH). The dual effects, the significant down-regulation of 14-3-3ε and the significant up-regulation of 14-3-3τ/θ at 2 h after... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Liver regeneration; 14-3-3 proteins; Western blotting. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500855 |
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| Shen,Cong-Cong; Kang,Yu-Huan; Yu,Lin; Cui,Dan-Dan; He,Yi; Yang,Jin-Liang; Gou,Lan-Tu. |
| BACKGROUND: Testis-expressed sequence 101 (TEX101) was found to be highly expressed in testis and involved in acrosome reaction in previous studies. Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been reported. In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed. RESULTS: Immunohistochemistry demonstrated intense staining of TEX101 in human testis tissues; however, its expression in 27 other types of normal human organs, including the ovary, was negligible. Higher expression of TEX101 was observed in the spermatocytes and spermatids of the testis, but relatively lower staining was detected in... |
| Tipo: Journal article |
Palavras-chave: Immunohistochemistry; TEX101; Seminoma; Testis; Western blotting. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100075 |
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| SOUTO, B. M.; VERZA, N. C.; OLIVEIRA, P. E. F.; BITTENCOURT, D. M. de C.; MADEIRA, L. M.; ANDRADE, A. C.; SILVA, F. R. da; LEWIS, R. V.; RECH FILHO, E. L.. |
| Aranhas produzem uma variedade de sedas que possuem extraordinárias propriedades moleculares e mecânicas. Utilizando seqüências expressas nas glândulas da seda, fomos capazes de identificar quatro proteínas produzidas pelas glândulas ampoladas (maior e menor), flageliforme e tubiliforme da espécie de aranha brasileira Nephilengys cruentata (Araneae: Nephilidae). As novas proteínas ídentificadas mostraram grande similaridade com outras sedas previamente descritas. Dentre essas sedas, a produzida pela glândula flageliforme (NCFlag-like) é extremamente elástica, o que facilita a captura de presas sem que ocorra o rompimento do fio. |
| Tipo: Parte de livro |
Palavras-chave: Ampola; Spider; Nephilengys cruentata; Glándulas de seda; Telarañas; Fibras animales; Proteínas de la seda; NCFlag-like; Ingeniería de proteínas; Propiedades mecánicas; Engenharia Genética; Aranha; Biologia Animal; Fibra Animal; Seda; Proteína; Propriedade Mecânica; Protein engineering; Araneae; Animal fibers; Silk glands; Webs; Mechanical properties; Silk proteins; Western blotting. |
| Ano: 2007 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/505926 |
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| Peron,G.; Fernandes,F.F.; Landgraf,T.N.; Martinez,R.; Panunto-Castelo,A.. |
| Paracoccidioides brasiliensis and P. lutzii are fungi that cause paracoccidioidomycosis (PCM), the most prevalent systemic mycosis in South America. For serological diagnosis, although 43-kDa glycoprotein (gp43) is regarded as highly specific for PCM, the occurrence of false negative reactions in sera from patients infected with P. lutzii suggests that preparation with only one antigen is not recommended. Heat shock proteins are feasible alternatives as a second antigen because they are often highly immunogenic. In this study, we evaluated the usefulness of recombinant 60-kDa heat shock protein from P. brasiliensis (rPbHsp60) for the serological diagnosis of PCM. Using western blotting assay, we observed that 77.3% of the sera from PCM patients were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paracoccidioidomycosis; Heat shock protein; Serodiagnosis; ELISA; Western blotting. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400705 |
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| NASCIMENTO, D. R.; SOLON, P. E.; BARROSO, M. L. da S.; PINHEIRO, R. R.; ELOY, A. M. X.. |
| A artrite encefalite caprina (CAE) é uma virose disseminada nos rebanhos caprinos, assemelha-se ao vírus da imunodeficiência humana e não possui cura ou vacina. Um dos métodos para prevenção está no diagnóstico precoce com a utilização de testes como Western Blotting (WB) e Nested-PCR (nPCR). No entanto, estes testes podem apresentar resultados falsos negativos, pois o vírus possui alta taxa de mutação, além apresentar latência. Objetivou-se validar o teste zimográfico através das metaloproteinases-2 (MMP-2) e metaloproteinases-9 (MMP-9), em animais leiteiros do Nordeste através da comparação com o WB e nPCR. |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: MMP 2; MMP 9; Artrite encefalite-caprina; CAE; Técnica zimográfica; Caprino; Doença Animal; Virose Animal; Diagnostico; Western blotting; Caprine arthritis-encephalitis; Diagnostic techniques. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102260 |
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| PEIXOTO, R. M.; SOUSA, A. L. M. de; ARAÚJO, J. F.; PINHEIRO, R. R.. |
| Background: Small ruminant lentivirus (SRLV) belong to genus Lentivirus, family Retroviridae. These viruses cause caprine arthritis encephalitis (CAE) and maedi visna (MV), infectious diseases that cause economic, production, and reproductive losses. There are no effective treatments or vaccines for these diseases. Thus, early detection via serology has great importance for control of SRLV. Therefore, the objective of this review is to demonstrate the potential of the western blot (WB) test as an immunodiagnostic test for SRLV. Review: In general, immunodiagnosis of SRLV is performed via agar gel immunodiffusion (AGID) and indirect enzyme linked immunosorbent assay (ELISA), which can detect antibodies in several different biological samples but is used... |
| Tipo: Artigo de periódico |
Palavras-chave: Diagnóstico sorológico; Lentivirose; Serological diagnosis; Lentiviruses; LVPR; SRLV; Ovino; Caprino; Imunoglobulina; Doença Animal; Sheep diseases; Goat diseases; Goats; Immunoglobulins; Serodiagnosis; Disease diagnosis; Western blotting; Caprine arthritis-encephalitis; Visna maedi virus. |
| Ano: 2021 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137467 |
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| Registros recuperados: 13 | |
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