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| SILVA, G. F. da; SANTOS, A. K. A.; ECHEVERRI, L. M. S.; SOUSA, B. A. de; VICENTE, M. C. de; NASCIMENTO, S. C. do; SILVA, F. N. da; BOGO, A.; LAU, D.; FERREIRA JÚNIOR, A.. |
| Abstract: In Brazil, wheat (Triticum aestivum) is cultivated mainly in the southern part. The soilborne wheat mosaic disease (SBWMD), caused by the wheat stripe mosaic virus (WhSMV), is one of the most important viruses in the region. Its diagnosis is based mainly on symptoms since molecular techniques are expensive, especially for RNA genomes viruses. However, serological tests using IgY immunoglobulins are highly efective in identifying viruses. This study aimed to produce and characterize a polyclonal antibody capable of interacting with the WhSMV capsid protein. The gene encoding the virus capsid protein was inserted into the expression vector pET-15b; the transformation and expression occurred in Escherichia coli BL21::DE3 in vitro. The recombinant... |
| Tipo: Artigo de periódico |
Palavras-chave: Benyviridae; WhSMV; Capsid protein; Diagnosis; IgY; Criação de frango; Trigo; Mosaico; Diagnostico; Vírus; Triticum aestivum WIS-2 virus; Chickens. |
| Ano: 2022 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1139239 |
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