| Registro completo |
| Provedor de dados: |
Agronomy
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| País: |
Brazil
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| Título: |
Ultrastructural and cytochemical analysis of physic nut callus tissue in response to different combinations of growth regulators
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| Autores: |
Santos, Dalilhia Nazaré dos
Nunes, Claudinéia Ferreira
Soares, Joyce Dória Rodrigues
Alves, Eduardo
Labory, Cláudia Regina Contijo
Pasqual, Moacir
Pio, Leila Aparecida Salles
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| Data: |
2015-08-03
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| Ano: |
2015
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| Palavras-chave: |
Jatropha curcas L.
Embryogenesis
Microscopy
Cytochemistry
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| Resumo: |
This study aimed to induce callus formation in Jatropha curcas L. and to evaluate the ultrastructure and cytochemical behavior of the calli. Calluses were induced with 2,4-D, picloram-PIC, kinetin (Kin) and BAP: (1) control; (2) 4.52 µM 2,4-D; (3) 9.04 µM 2,4-D; (4) 4.14 µM PIC; (5) 8.28 µM PIC; (6) 4.52 µM 2,4-D + 2.32 µM KIN; (7) 9.04 µM 2,4-D + 4.64 µM KIN; (8) 4.14 µM PIC + 2.32 µM KIN; (9) 8.28 µM PIC + 4.64 µM KIN; (10) 4.52 µM 2,4-D + 2.22 µM BAP; (11) 9.04 µM 2,4-D + 4.44 µM BAP; (12) 4.14 µM PIC + 2.22 µM BAP and (13) 8.28 µM PIC + 4.44 µM BAP. It was evaluated the percent coverage of the explants by callus (% CEC) and performed scanning electron microscopy (SEM) and acetocarmine/Evans blue double staining to analyze the embryogenic potential of the calli. As shown by scanning electron microscopy and acetocarmine/Evans blue staining, we found that J. curcas callus formation was optimal with 4.52 µM of 2,4-D.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19745
10.4025/actasciagron.v37i3.19745
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| Editor: |
Universidade Estadual de Maringá
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| Relação: |
http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19745/pdf_86
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| Formato: |
application/pdf
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| Fonte: |
Acta Scientiarum. Agronomy; v. 37, n. 3 (2015); 355-359
Acta Scientiarum. Agronomy; v. 37, n. 3 (2015); 355-359
1807-8621
1679-9275
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