Registro completo |
Provedor de dados: |
Agronomy
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País: |
Brazil
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Título: |
QTLs identification for characteristics of the root system in upland rice through DNA microarray
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Autores: |
Terra, Thiago Gledson Rios
Rodrigues, Haroldo Silva
Rangel, Paulo Hideo Nakano
Tomaz, Rafael Simões
Cruz, Cosme Damião
Borém, Aluízio
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Data: |
2016-09-02
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Ano: |
2016
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Palavras-chave: |
5.01.03.05-9 genomic
Oryza sativa L.
DNA chip
QTL mapping
Abiotic stress Melhoramento Vegetal
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Resumo: |
The aim of this work is the construction of a genetic map and identification of quantitative trait loci (QTLs) that control characteristics of the root system of rice. We evaluated a F2:3 population composed of 150 families from the cross between the varieties IAC 165 × BRS Primavera. Genotyping was performed in the F2 population using 3,742 SNP (Single Nucleotide Polymorphism) markers. The evaluation of the root system in the F3 population was performed through a large-scale phenotyping method based on image generation with a CI-600 root scanner and on quantification through the WinRhizo® software. The experiment was arranged in a randomized block design with three replications performed under greenhouse. The variables analyzed were root length, root surface area and root volume at the depths of 5 to 25 cm and 25 to 45 cm. The SNP markers analysis allowed the construction of the genetic map with a full length of 1424 cM. The linkage group with the largest coverage area was number 3 with 270 cM (100 SNPs), followed by linkage group 1 with 249 cM (170 SNPs) and linkage group 2 with 163 cM (99 SNPs). The genetic analysis allowed the detection of QTLs for all the characteristics.
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Tipo: |
Info:eu-repo/semantics/article
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Idioma: |
Inglês
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Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/30534
10.4025/actasciagron.v38i4.30534
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Editor: |
Universidade Estadual de Maringá
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Relação: |
http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/30534/pdf
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Formato: |
application/pdf
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Fonte: |
Acta Scientiarum. Agronomy; v. 38, n. 4 (2016); 457-466
Acta Scientiarum. Agronomy; v. 38, n. 4 (2016); 457-466
1807-8621
1679-9275
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Direitos: |
Direitos autorais 2016 Acta Scientiarum. Agronomy
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