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Provedor de dados: |
Agronomy
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País: |
Brazil
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Título: |
Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841
Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841
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Autores: |
Vieira, Viviane Cristina; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista
Alves, Pedro Luís da Costa Aguiar; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista
Picchi, Simone Cristina; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista
Lemos, Manoel Victor Franco; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista
Sena, Janete Apparecida Desidério; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista
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Data: |
2010-04-29
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Ano: |
2010
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Palavras-chave: |
Digitaria
PsbA gene
RAPD
Sequencing
Triazine
Weed Digitaria
PsbA gene
RAPD
Sequencing
Triazine
Weed
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Resumo: |
Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction – Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psb A gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn.
Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction – Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psb A gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn.
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Idioma: |
Português
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Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841
10.4025/actasciagron.v32i2.3841
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Editor: |
Universidade Estadual de Maringá
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Formato: |
application/pdf
application/pdf
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Fonte: |
Acta Scientiarum. Agronomy; v. 32, n. 2 (2010); 255-261
Acta Scientiarum. Agronomy; v. 32, n. 2 (2010); 255-261
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Direitos: |
DECLARATION OF ORIGINALITY AND CESSATION OF COPYRIGHTI Declare that current article is original and has not been submitted for publication, in part or in whole, to any other national or international journal. I also declare that once published in the Acta Scientiarum. Agronomy, a publication of the Universidade Estadual de Maringá, it will not be submitted by me or by any co-author to any other scientific communication mean. In my name and in the name of co-authors, I shall cede the copyright of the above mentioned article to the Universidade Estadual de Maringá and I declare that I know that the non-observance of this norm may make me liable for the penalties contemplated in the Law for the Protection of Authors’ Rights (Act 9609 of the 19th February 1998).
DECLARACIÓN DE ORIGINALIDAD Y CESIÓN DE DERECHOS DE AUTOR Declaro que el presente artículo es original, no fue sometido a la publicación en cualquier otro periódico nacional o internacional, sea en parte o en su totalidad. Declaro, aun, que una vez publicado en la revista Acta Scientiarum. Agronomy, editado por la Universidad Estadual de Maringá, éste jamás será sometido por mí o por cualquier uno de los demás coautores a cualquier otro periódico. A través de este instrumento, en mi nombre y en nombre de los demás coautores, por ventura existentes, cedo los derechos de autor del referido artículo a la Universidad Estadual de Maringá y declaro estar informado de que la no observancia de este compromiso someterá al infractor las sanciones y penas previstas en la Ley de Protección de Derechos de Autor (Nº9609, de 19/02/98).
DECLARAÇÃO DE ORIGINALIDADE E CESSÃO DE DIREITOS AUTORAISDeclaro que o presente artigo é original, não tendo sido submetido à publicação em qualquer outro periódico nacional ou internacional, quer seja em parte ou em sua totalidade. Declaro, ainda, que uma vez publicado na revista Acta Scientiarum. Agronomy, editada pela Universidade Estadual de Maringá, o mesmo jamais será submetido por mim ou por qualquer um dos demais co-autores a qualquer outro meio de divulgação científica. Através deste instrumento, em meu nome e em nome dos demais co-autores, porventura existentes, cedo os direitos autorais do referido artigo à Universidade Estadual de Maringá e declaro estar ciente de que a não observância deste compromisso submeterá o infrator a sanções e penas previstas na Lei de Proteção de Direitos Autorias (Nº9609, de 19/02/98).
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