| Registro completo |
| Provedor de dados: |
Agronomy
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| País: |
Brazil
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| Título: |
Cryopreservation of the mangaba tree (Hancornia speciosa Gomes): a protocol for long-term storage
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| Autores: |
Santos, Paulo Augusto Almeida
Paiva, Renato
Silva, Luciano Coutinho
Souza, Ana Cristina de
Santana, Marlucia Cruz de
Silva, Diogo Pedrosa Corrêa da
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| Data: |
2015-08-03
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| Ano: |
2015
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| Palavras-chave: |
5.00.00.00-4 - ciências agrárias
2.03.03.00-9 - fisiologia vegetal
Native species
In vitro conservation
Recalcitrant seeds
Plant genetic diversity conservation
Vitrification
Droplet vitrification Conservação in vitro
Criopreservação vegetal
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| Resumo: |
The aim of this study was to evaluate the efficiency of vitrification and droplet vitrification for the cryopreservation of Hancornia speciosa shoot tips. The shoot tips were subjected to four different periods of exposure (15, 30, 45 and 60 min.) to plant vitrification solution 2 (PVS2) before plunging into liquid nitrogen. We evaluated the regrowth of H. speciosa shoot tips that were cryopreserved by the classical vitrification technique and by the droplet vitrification technique. Shoot tips were submitted to different periods of pre-culture (absence, 24 or 48h) in a medium containing 0.3 M sucrose prior to cryopreservation. With a PVS2 exposure period of 60 min., significant differences were observed between the two techniques used in this study with respect to regrowth rates; however, both cryopreservation methods allowed for a shoot tip regrowth rate of over 70%. Use of a preculture step increased the regrowth rate of cryopreserved shoot tips. Shoot tip cryopreservation techniques employing vitrification and droplet vitrification proved to be viable for the long-term storage of mangaba tree samples.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19573
10.4025/actasciagron.v37i3.19573
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| Editor: |
Universidade Estadual de Maringá
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| Relação: |
http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19573/pdf_78
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| Formato: |
application/pdf
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| Fonte: |
Acta Scientiarum. Agronomy; v. 37, n. 3 (2015); 289-296
Acta Scientiarum. Agronomy; v. 37, n. 3 (2015); 289-296
1807-8621
1679-9275
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