| Registro completo |
| Provedor de dados: |
Rev Salud Anim.
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| País: |
Cuba
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| Título: |
Evaluation of simplified DNA extraction methods for Streptococcus suis typing
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| Autores: |
Espinosa,Ivette
Báez,M
Percedo,María Irian
Martínez,Siomara
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| Data: |
2013-04-01
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| Ano: |
2013
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| Palavras-chave: |
Streptococcus suis
Direct colony PCR
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| Resumo: |
Streptococcus suis is a gram-positive bacterium that causes serious diseases in pigs and in humans with occupational risk. The DNA extraction methods for amplification of gene fragments by PCR for typing S.suis may be complex, and expensive chemical reagents and time consuming. The aim of this study was to evaluate a method for the rapid release of the genomic DNA from S.suis colonies by using a physical method based on heating and freezing; in this case, temperatures of 100ºC and 95ºC were tested. The results showed that DNA extraction directly from colonies by heating at 100ºC could be useful for an easy genotyping of S.suis strains in a short time, while 95ºC was not sufficient for DNA release. The detection limit of the PCR assay using DNA obtained by chemical purification was 0.5ng; considering the size of S.suis genome, it is possible to estimate that an adequate amount of cells are in a single S.suis colony to ensure the sensitivity of the PCR assay.
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| Tipo: |
Journal article
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| Idioma: |
Inglês
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| Identificador: |
http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2013000100009
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| Editor: |
Centro Nacional de Sanidad Agropecuaria
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| Formato: |
text/html
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| Fonte: |
Revista de Salud Animal v.35 n.1 2013
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