| Registro completo |
| Provedor de dados: |
Trop. Plant Pathol.
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| País: |
Brazil
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| Título: |
Transgenic passionfruit expressing RNA derived from Cowpea aphid-borne mosaic virus is resistant to passionfruit woodiness disease
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| Autores: |
Alfenas,Poliane F.
Braz,Antonio Sérgio K.
Torres,Leonardo B.
Santana,Enilton N.
Nascimento,Ana Verônica S. do
Carvalho,Murilo G. de
Otoni,Wagner C.
Zerbini,F. Murilo
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| Data: |
2005-02-01
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| Ano: |
2005
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| Palavras-chave: |
Passionfruit woodiness virus
PWV
Potyvirus
Resistance
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| Resumo: |
Sixteen transgenic yellow passionfruit (Passiflora spp.) plants (R0) were obtained which express a non-translatable transgenic RNA corresponding to the 3' region of the NIb gene and the 5' region of the CP gene, derived from the genome of a Brazilian isolate of Cowpea aphid-borne mosaic virus (CABMV). The transgenic plants were propagated by stem cuttings and challenged by sap inoculation with isolates CABMV-MG1 and CABMV-PE1. One transgenic plant (TE5-10) was resistant to the isolate CABMV-MG1, but susceptible to CABMV-PE1. The remaining transgenic plants developed systemic symptoms, equal to non-transformed plants, when inoculated with either isolate. The absence of virus in TE5-10 plants was confirmed by indirect ELISA. Transcription analysis of the transgene demonstrated that the TE5-10 plant did not accumulate transgenic mRNA, even before inoculation. After inoculation, viral RNA was only detected in plants inoculated with CABMV-PE1. These results confirm that the transgenic plant TE5-10 is resistant to isolate CABMV-MG1, and suggest that the resistance mechanism is post-transcriptional gene silencing, which is already activated in the transgenic plants before virus inoculation.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000100006
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| Editor: |
Sociedade Brasileira de Fitopatologia
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| Relação: |
10.1590/S0100-41582005000100006
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| Formato: |
text/html
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| Fonte: |
Fitopatologia Brasileira v.30 n.1 2005
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| Direitos: |
info:eu-repo/semantics/openAccess
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